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极危物种——南川木波罗遗传多样性ISSR分析 认领 被引量:2

ISSRanalysis of the genetic diversity of Artocarpus nanchuanensis, a critically endangered species, in Nanchuan District, Chongqing
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摘要 本研究利用ISSR-PCR分子标记技术分析研究了重庆市七个地区野生生长的46株南川木波罗(Artocarpus nanchuanensis)样品的遗传多样性.利用筛选出的12条UBC引物共扩增出了117条DNA片段,其中多态性片段为113条,多态条带百分率为96.58%.NTSYS~pc2.1软件分析表明:七个地区南川木波罗之间的遗传相似性介于0.062~0.989之间,并用UPGMA法构建了系统树.结果表明该市野生生长的南川木波罗既有相同的遗传背景,又存在较明显的差异.46份材料划分为4类,聚类基本符合地理来源相近的材料聚为一类,呈现出一定的地域性分布规律. Genetic diversity of 46 strainswild Artocarpus nanchuanensis from seven areas of Chongqing was preliminary analyzed using inter-simple sequence repeat (ISSR) PCR molecular marker technology. The selected 12 pairs of UBC primers were used in this research, and a total of 117 DNA fragments were obtained, of which 113 were polymorphic fragments, and the percentage of polymorphic bands was 96.58%. The analysis by NTSYS pc2.1 software showed that the genetic similarity among Artocarpus nanchuanensis from the seven areas was ranged from 0.062~0.989, and the dendrogram was constructed through UPGMA method. The results revealed that these Artocarpus heterophyllus Lam. have a similar genetic background with obvious differences. These Artocarpus heterophyllus Lam. were obviously divided into four groups, and the cluster is in line with the similar geographical origin of materials, showing a certain regional distribution.
作者 胡连清 王清明 丁显平 谢依婷 张顺 包善飞 陈祖翼 HU Lian-Qing1, WANG Qing-Ming1, DING Xian-Ping1,2, XIE Yi-Ting1 ZHANG Shun 1, BAO Shan-Fei2, CHEN Zu-Yi1,2 (1. College of Life Science Sichuan University, Bio-resource Research and Utilization Joint Key Laboratory of Sichuan-Chongqing, Chengdu 610064, China; 2. Biotechnology academy of Nanchuan, Nanchuan 408400, China)
出处 《四川大学学报:自然科学版》 CAS CSCD 北大核心 2018年第4期865-872,共8页 Journal of Sichuan University (Natural Science Edition)
基金 重庆市科委科技研发平台专项(cstc2017yfpt-xxyfig0088)
关键词 南川木波罗 ISSR 遗传多样性 遗传相似系数 遗传距离 Artocarpu sheterophyllus Lam. Inter-simple sequence repeat (ISSR) Genetic diversity Genetic simiarity coefficent Genetic distance
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