Objective To study the preparation of tumor inhibiting polypeptides for injection （TIPI）. Methods The dissolvable collagen was extracted from the suckling pig liver by the biochemical approach, TIPI was prepared by the pepsin hydrolysis. The product was checked on the drafting quality criteria. The effect of TIPI on the HepA hepatoma, H22 hepatoma and S180 fibrosarcoma were investigated. Results The product＇s quality reached the drafting quality criteria showed that the production technological line of TIPI was suitable. The results showed that TIPI could apparently inhibit the growth of HepA hepatoma in the tumor-bearing mice with the inhibitory rate of 43.26%, 55.06%,74.71%,40.47% from the doses of 25, 50, 100, 200 mg/（kg· d） TIPI; and inhibit the growth of S180 fibrosarcoma with the inhibitory rate of 53.87%,76. 53%,58.20% from the respective doses of 50, 100,200mg/（kg· d） TIPI, the relative inhibitory rate of the positive adriamycin [2 mg/（kg· d）] control was 62.41%;TIPI could apparently inhibit the growth of H22 hepatoma transplanted in the mice in a dose dependant way with the inhibitory rate of 48.1%, 60.0%, 74.8% from the respective doses of 50, 100, 200 mg/（kg· d） TIPI, and the relative inhibitory rate of the positive adriamycin [20mg/（kg· d）] control was 63.7%. Conclusion TIPI produced by the biochemical approach is qualified for injection and a large scale production, TIPI can apparently inhibit the growth of tumor transplanted in the mice.
tumor inhibiting polypeptide