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阿尔茨海默病药物治疗的中英指南对比及解析 认领
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作者 张小林 曾嘉炜 +1 位作者 陈孝 元刚 《中国全科医学》 CAS 北大核心 2021年第12期1454-1458,共5页
阿尔茨海默病(AD)的临床症状多样且以认知功能损害为主,给患者和家庭带来了巨大的精神压力和沉重的经济负担。目前尚无根治AD的药物,临床上常采用胆碱酯酶抑制剂和美金刚改善AD症状,但其疗效不一且安全性和成本效果性存在差异。为给患... 阿尔茨海默病(AD)的临床症状多样且以认知功能损害为主,给患者和家庭带来了巨大的精神压力和沉重的经济负担。目前尚无根治AD的药物,临床上常采用胆碱酯酶抑制剂和美金刚改善AD症状,但其疗效不一且安全性和成本效果性存在差异。为给患者选择安全、有效、经济的用药方案,中国和英国分别在2018年更新发表了AD药物治疗相关的指南。中国发布《2018中国痴呆与认知障碍诊治指南(二):阿尔茨海默病诊治指南》在AD的诊断、药物治疗和临床疗效进行了阐述,英国国家卫生与临床优化研究所(NICE)发布的Donepezil,galantamine,rivastigmine and memantine for the treatment of Alzheimer's disease进行AD常用治疗药物的临床效果和药物经济学方面的分析。本文重点比较两个指南在药物方面的异同之处,旨在为临床医生和基层医生开具AD治疗药物时提供更多参考。 展开更多
关键词 阿尔茨海默病 治疗 药物疗法 指南 解读
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A targeting black phosphorus nanoparticle based immune cells nanoregulator for photodynamic/photothermal and photo-immunotherapy 认领
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作者 Xiaoge Zhang Junjie Tang +3 位作者 Chao Li Yao Lu Lili Cheng Jie Liu 《生物活性材料》 SCIE 2021年第2期472-489,共18页
Photo-immunotherapy is a novel therapeutic approach against malignant tumors with minimal invasiveness.Herein,a targeting multifunctional black phosphorus(BP)nanoparticle,modified by PEGylated hyaluronic acid(HA),was ... Photo-immunotherapy is a novel therapeutic approach against malignant tumors with minimal invasiveness.Herein,a targeting multifunctional black phosphorus(BP)nanoparticle,modified by PEGylated hyaluronic acid(HA),was designed for photothermal/photodynamic/photo-immunotherapy.In vitro and in vivo assays indicated that HA-BP nanoparticles possess excellent biocompatibility,stability,and sufficient therapeutic efficacy in the combined therapy of photothermal therapy(PTT)and photodynamic therapy(PDT)for cancer therapy.Moreover,the results of in vitro showed that HA-BP down-regulated the expression of CD206(M2 macrophage marker)by 42.3%and up-regulated the ratio of CD86(M1 macrophage marker)by 59.6%,indicating that HABP nanoparticles have functions in remodeling tumor associated macrophages(TAMs)phenotype(from protumor M2 TAMs to anti-tumor M1 macrophages).Fluorescence(FL)and photoacoustic(PA)multimodal imaging confirmed the selective accumulation of HA-BP in tumor site via both CD44^+mediated active targeting and passive EPR effect.In vitro and in vivo studies suggested that the combined therapy of PDT,PTT and immunotherapy using HA-BP could not only significantly inhibit original tumor but also induce immunogenic cell death(ICD)and release Damage-associated molecular patterns(DAMPs),which could induce maturation of dendritic cells(DCs)and activate effector cells that robustly evoke the antitumor immune responses for cancer treatment.This study expands the biomedical application of BP nanoparticles and displays the potential of modified BP as a multifunctional therapeutic platform for the future cancer therapy. 展开更多
关键词 Black phosphorus Tumor associated macrophages Photothermal therapy Photodynamic therapy IMMUNOTHERAPY
Photosensitizer Nanoparticles Boost Photodynamic Therapy for Pancreatic Cancer Treatment 认领
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作者 Huanyu Yang Renfa Liu +2 位作者 Yunxue Xu Linxue Qian Zhifei Dai 《纳微快报:英文版》 SCIE EI CAS 2021年第2期246-261,共16页
Patients with pancreatic cancer(PCa)have a poor prognosis apart from the few suitable for surgery.Photodynamic therapy(PDT)is a minimally invasive treatment modality whose efficacy and safety in treating unresectable ... Patients with pancreatic cancer(PCa)have a poor prognosis apart from the few suitable for surgery.Photodynamic therapy(PDT)is a minimally invasive treatment modality whose efficacy and safety in treating unresectable localized PCa have been corroborated in clinic.Yet,it suffers from certain limitations during clinical exploitation,including insufficient photosensitizers(PSs)delivery,tumor-oxygenation dependency,and treatment escape of aggressive tumors.To overcome these obstacles,an increasing number of researchers are currently on a quest to develop photosensitizer nanoparticles(NPs)by the use of a variety of nanocarrier systems to improve cellular uptake and biodistribution of photosensitizers.Encapsulation of PSs with NPs endows them significantly higher accumulation within PCa tumors due to the increased solubility and stability in blood circulation.A number of approaches have been explored to produce NPs co-delivering multi-agents affording PDT-based synergistic therapies for improved response rates and durability of response after treatment.This review provides an overview of available data regarding the design,methodology,and oncological outcome of the innovative NPs-based PDT of PCa. 展开更多
关键词 Photodynamic therapy PHOTOSENSITIZER NANOPARTICLE Pancreatic cancer Combined therapy
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The secretome of endothelial progenitor cells: a potential therapeutic strategy for ischemic stroke 认领
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作者 Mansour Alwjwaj Rais Reskiawan AKadir Ulvi Bayraktutan 《中国神经再生研究:英文版》 SCIE CAS 2021年第8期1483-1489,共7页
Ischemic stroke continues to be a leading cause of mortality and morbidity in the world. Despite recent advances in the field of stroke medicine, thrombolysis with recombinant tissue plasminogen activator remains as t... Ischemic stroke continues to be a leading cause of mortality and morbidity in the world. Despite recent advances in the field of stroke medicine, thrombolysis with recombinant tissue plasminogen activator remains as the only pharmacological therapy for stroke patients. However, due to short therapeutic window(4.5 hours of stroke onset) and increased risk of hemorrhage beyond this point, each year globally less than 1% of stroke patients receive this therapy which necessitate the discovery of safe and efficacious therapeutics that can be used beyond the acute phase of stroke. Accumulating evidence indicates that endothelial progenitor cells(EPCs), equipped with an inherent capacity to migrate, proliferate and differentiate, may be one such therapeutics. However, the limited availability of EPCs in peripheral blood and early senescence of few isolated cells in culture conditions adversely affect their application as effective therapeutics. Given that much of the EPC-mediated reparative effects on neurovasculature is realized by a wide range of biologically active substances released by these cells, it is possible that EPC-secretome may serve as an important therapeutic after an ischemic stroke. In light of this assumption, this review paper firstly discusses the main constituents of EPC-secretome that may exert the beneficial effects of EPCs on neurovasculature, and then reviews the currently scant literature that focuses on its therapeutic capacity. 展开更多
关键词 ANTIOXIDANTS cell-based therapy cell-free therapy endothelial progenitor cells inflammatory cytokines regenerative medicine SECRETOME STROKE vasodegeneration vasorepair
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脊髓损伤再生修复中的问题与挑战 认领
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作者 田婷 李晓光 《中国组织工程研究》 CAS 北大核心 2021年第19期3039-3048,共10页
背景:脊髓损伤导致严重的感觉、运动功能障碍,反射异常以及自主神经功能紊乱。目前脊髓损伤尚无有效的临床治疗措施。近年来,研究人员开发出多种新型治疗方案,为临床治疗脊髓损伤开辟了新途径。目的:探讨脊髓损伤病理生理学过程,总结脊... 背景:脊髓损伤导致严重的感觉、运动功能障碍,反射异常以及自主神经功能紊乱。目前脊髓损伤尚无有效的临床治疗措施。近年来,研究人员开发出多种新型治疗方案,为临床治疗脊髓损伤开辟了新途径。目的:探讨脊髓损伤病理生理学过程,总结脊髓损伤再生修复研究进展,分析临床转化现状与挑战,展望脊髓损伤治疗未来发展趋势。方法:检索PubMed数据库2003至2020年发表的相关文章,检索词为“spinal cord injury,neuroplasticity,functional recovery,therapeutic strategy,combinatory therapies,clinical translation”。初检文章398篇,经过筛选、整理,最终纳入116篇文章进行分析、总结。结果与结论:脊髓损伤的病理生理过程包括原发性损伤和继发性损伤。针对脊髓损伤的病理生理过程,脊髓损伤的潜在治疗策略包括:①增强神经保护:减少继发性损伤;②促进再生修复:激活神经元内在再生能力、改善微环境、细胞移植、神经调控与康复训练、脑机接口、联合治疗。脊髓损伤的病理过程十分复杂,单一治疗措施不足以促进人类脊髓损伤后神经再生和功能恢复。联合治疗通过解决脊髓损伤的多个病理过程,已经证明比单一治疗更为有效。未来临床试验应该加强多学科交流与合作,以确定最佳组合方案,促进脊髓损伤患者功能恢复。 展开更多
关键词 干细胞 脊髓损伤 神经可塑性 功能 治疗 联合治疗 临床转化 综述
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Cost-effectiveness of endoscopic ultrasound-guided coils plus cyanoacrylate injection compared to endoscopic cyanoacrylate injection in the management of gastric varices 认领
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作者 Carlos Robles-Medranda Joao Autran Nebel +5 位作者 Miguel Puga-Tejada Roberto Oleas Jorge Baquerizo-Burgos Jesenia Ospina-Arboleda Manuel Valero Hannah Pitanga-Lukashok 《世界胃肠内镜杂志:英文版(电子版)》 CAS 2021年第1期13-23,共11页
BACKGROUND Cyanoacrylate(CYA)injection can be performed using a standard upper endoscopy technique or under endoscopic ultrasound(EUS)guidance alone or in combination with coils.There is little information available o... BACKGROUND Cyanoacrylate(CYA)injection can be performed using a standard upper endoscopy technique or under endoscopic ultrasound(EUS)guidance alone or in combination with coils.There is little information available on the economic impact of these treatment methods.AIM To compare the cost-effectiveness of treating gastric varices by CYA injection via upper endoscopy vs coils plus CYA guided by EUS.METHODS This was an observational,descriptive,and retrospective study.Patients were allocated into two groups:A CYA group and coils plus CYA group.The baseline characteristics were compared,and a cost analysis was performed.RESULTS Overall,36 patients were included(19 in the CYA group and 17 in the coils+CYA group).All patients in the CYA group had acute bleeding.They underwent a higher mean number of procedures(1.47 vs 1,P=0.025),and the mean volume of glue used was 2.15 vs 1.65 mL,P=0.133.The coils+CYA group showed a higher technical success rate(100%vs 84.2%),with a complication rate similar to the CYA group.The majority of CYA patients required hospitalization,and although the mean total per procedure cost was lower(United States$1350.29 vs United States$2978),the mean total treatment cost was significantly different(United States$11060.89 for CYA vs United States$3007.13 for coils+CYA,P=0.03).CONCLUSION The use of EUS-guided coils plus cyanoacrylate is more cost-effective than cyanoacrylate injection when the total costs are evaluated.Larger,randomized trials are needed to validate the cost-effectiveness of the EUS-guided approach to treat gastric varices. 展开更多
关键词 COST-EFFECTIVENESS Endoscopic ultrasound-guided therapy Gastric varices Gastrointestinal bleeding Hemostasis THERAPY
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中国黑素瘤研究进展与新治疗策略 认领
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作者 高天文 郭伟楠 《中华皮肤科杂志》 CAS 北大核心 2021年第1期27-32,共6页
尽管黑素瘤发病率正快速增长,但其在中国所占比例低,肿瘤科医生关注少。黑素瘤患者首诊科室主要是皮肤科,皮肤科医生能综合分析临床诊断、病理诊断、外科治疗和药物治疗,对黑素瘤的诊治具有独特优势。中国学者近年在黑素瘤的细胞死亡调... 尽管黑素瘤发病率正快速增长,但其在中国所占比例低,肿瘤科医生关注少。黑素瘤患者首诊科室主要是皮肤科,皮肤科医生能综合分析临床诊断、病理诊断、外科治疗和药物治疗,对黑素瘤的诊治具有独特优势。中国学者近年在黑素瘤的细胞死亡调控、表观遗传学修饰、靶向药物耐药、肿瘤微环境和肿瘤免疫调控等方面已获得很大进展。中国独有的一类新药干扰素α-1b不仅可用于Ⅱ、Ⅲ期高危黑素瘤的辅助治疗,对Ⅳ期黑素瘤也有很好的疗效,不良反应远低于干扰素α-2b。与白色人种不同,亚洲人皮肤黑素瘤以肢端型及黏膜型为主。以干扰素α-1b为基础制定联合治疗策略,探索其与程序性死亡受体-配体1抑制剂、靶向药物或血管生成抑制剂等的联合治疗方案,正在为挽救Ⅳ期黑素瘤患者带来希望。 展开更多
关键词 痣和黑素瘤 治疗 干扰素Α 分子靶向治疗 程序性细胞死亡受体1 免疫检查点抑制剂
文章速递Intrauterine Adhesions (IUAs) or Asherman’s Syndrome (AS) and the Stem Cells Treatment: A Systemic Review and Meta-Analysis 认领
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作者 Sichen Zhang Ying Mao +4 位作者 Xiaodong Zhao Huiwen Zhang Min Ma Xingbo Long Shaowei Wang 《生物科学与医学(英文)》 2021年第1期105-118,共14页
<strong>Background:</strong> Intrauterine Adhesions (IUAs) or Asherman’s Syndrome (AS) usually contains symptoms such as decreased menstrual flow or even amenorrhea, chronic pelvic pain, recurrent abortio... <strong>Background:</strong> Intrauterine Adhesions (IUAs) or Asherman’s Syndrome (AS) usually contains symptoms such as decreased menstrual flow or even amenorrhea, chronic pelvic pain, recurrent abortion and infertility. The current treatment of IUAs includes hysteroscopic adhesiolysis, oral hormone and biological barriers, but each of them has limitation. Stem cell therapy may be an expanding field seeking for therapy in IUAs. <strong>Objective: </strong>We will discuss current advances in stem cell therapy as a treatment for endometrial pathophysiology. <strong>Materials and Methods:</strong> We search on PubMed, Embase and Cochrane library and select several keywords on researches, then review the cell biology theories and animal experiments, finally do meta-analysis in human clinical trials. <strong>Results: </strong>77 articles on PubMed, 71 articles on Embase and 17 articles on Cochrane Library, as a result, 37 articles are included under the criteria, which are intrauterine adhesions (IUAs), Asherman’s Syndrome (AS), cell therapy, stem cells, bone marrow stem cells, clinical trials, recent 10 years and human or animal experiments. The included criteria: original articles, cohort study, case control study, animal experiments, human clinical trials, high quality, 10 years recent. The excluded articles are case reports, meeting reports, low quality or more than 10 years ago. <strong>Conclusion:</strong> Stem cell may be a new therapeutic schedule for IUAs in the future clinical treatment, but it is necessary to compare it with traditional therapy such as oral hormone, also the development of random clinical tests should proceed. For clinical treatment on IUAs, stem cells could be a new choice. 展开更多
关键词 Intrauterine Adhesions IUA Asherman’s Syndrome Stem Cells Therapy Infertility Meta-Analysis
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文章速递Chordoma Sociodemographic, Clinical and Therapeutic Aspects in National Institute of Oncology Rabat Morocco: A Report of 9 Cases 认领
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作者 Gaël Kietga Patricia Agbanglanon +3 位作者 Bertrand Compaore Evrard Seka Amine Lachgar Noureddine Benjaafar 《癌症治疗(英文)》 2021年第1期47-56,共10页
<strong>Introduction:</strong> <span><span><span style="font-family:""><span style="font-family:Verdana;">Chordoma is a rare bone tumor, which develops main... <strong>Introduction:</strong> <span><span><span style="font-family:""><span style="font-family:Verdana;">Chordoma is a rare bone tumor, which develops mainly from the sacrum, the base of the skull, or the spine. Surgery + radiotherapy (if necessary) is the standard treatment. Data on chordoma are scarce in this re</span><span style="font-family:Verdana;">gion, and thus, here we summarized 9 patients with this tumor whom we</span><span style="font-family:Verdana;"> treated in this institute. </span><b><span style="font-family:Verdana;">Material: </span></b><span style="font-family:Verdana;">Nine chordoma patients were summarized, who were treated in National Institute of Oncology in Rabat between 2013 </span><span style="font-family:Verdana;">and 2018. We retrieved data from medical charts and analyzed the clinical</span><span style="font-family:Verdana;"> characteristics of this tumor. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">The average age was 49 years (range: 29 </span><span style="font-family:Verdana;">- 72), with male: female of 3:6. The manifestation-diagnosis time was 4</span><span style="font-family:Verdana;"> months (range: 2 - 14). Regarding the tumor location, lumbosacral spine;5, the skull;4. Mass was evident in 6. Signs of locoregional compressions (paraparesis or tetraparesis) were observed in 3. As for treatment, a partial tumor excision was performed in 8, with 3 patients undergoing a wide excision. Radiotherapy was done;3 patients with a dose of 46 Gy, 3 patients with 66 Gy, 1 patient with 50 Gy, and 1 patient with a 16 Gy gamma radio-knife in a single session. 4 patients with a dose of 46 Gy, 2 patients with 60 Gy, and 1 patient with a 16 Gy gamma radio-knife in a single session. Of 9, 4 patients had good locoregional control whereas 5 patients had local recurrence. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">Chordoma is a predominantly local aggressive tumor with low metastatic potential. The surgical excision remains the main prognostic factor. Advances in radiotherapy may improve local control. These data are of use in management of this tumor in Rabat (Morocco).</span></span></span></span> 展开更多
关键词 Chordoma Diagnosis Radiation Therapy Morocco
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文章速递Prenatal Diagnosis and Management of Fetal Goiter Treated Successfully with Intra-Amniotic Levothyroxine 认领
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作者 Valentina Corda Cristina Peddes +1 位作者 Ambra Iuculano Giovanni Monni 《妇产科期刊(英文)》 2021年第1期48-52,共5页
Goiter is an enlargement of the thyroid gland which can be associated with a number of complications both for the mother and the fetus. A 34-year-old pregnant woman with normal thyroid function was referred to our Dep... Goiter is an enlargement of the thyroid gland which can be associated with a number of complications both for the mother and the fetus. A 34-year-old pregnant woman with normal thyroid function was referred to our Department of Obstetrics and Gynecology at Microcitemico Pediatric Hospital, Cagliari, for suspected fetal goiter at 32 gestational weeks. The case was monitored regularly by ultrasound and treated successfully with intra-amniotic levothyroxine (L-T4) administration. Fetal goiter was observed to decrease after this treatment and the thyroid ultrasound findings were also normal both at birth and in subsequent follow-ups. Our case report confirms the feasibility of conservative treatment with L-T4, which can effectively prevent complications related to fetal goiter. 展开更多
关键词 Fetal Goiter Prenatal Diagnosis Prenatal Therapy Amniocentesis Fetal Thyroid Fetal Ultrasound Levothyroxine (L-T4)
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文章速递Importance of Pharmacotherapeutic Follow-Up in Odontopediatrics 认领
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作者 Cristina Janeth Montañez-Sosa César Gaitán-Fonseca +4 位作者 María Argelia López-Luna Carlos Bermúdez-Jiménez Jesús Luego-Ferreira Silverio Frausto-Esparza Luis Alejandro Aguilera-Galavíz 《口腔学期刊(英文)》 2021年第1期1-13,共13页
The most common cause of admission to the Pediatric Dentistry service is dental pain, active deafness and an oppressive sensation that is sometimes<span> throbbing and burning. Pharmacotherapy in Pediatric ... The most common cause of admission to the Pediatric Dentistry service is dental pain, active deafness and an oppressive sensation that is sometimes<span> throbbing and burning. Pharmacotherapy in Pediatric Dentistry must be effective, safe, and rational in neonates, infants, and children. The pharmacotherapeutic follow-up of pediatric patients from Integral Clinic of the Odontopediatric Specialty</span><span> </span><span>(CLIO) and Kindergarten Clinic (CLIJANI), Autonom</span><span>ous University of Zacatecas</span><span> </span><span>(UAZ) was carried out. Through an observa</span><span>tion</span><span>al, exploratory, and cross-sectional study, 23 patients from the Pediatric Dentistry Specialty Clinic (CLIO/UAO/UAZ) and 35 clinical records of patients </span><span>from CLIJANI, from August 2019-2020, were analyzed.</span><span> </span><span>Prior authorization</span><span> for </span><span>the patient, an interview, and pharmacotherapeutic follow-up were per</span><span>formed, recording data on the indicated drug, the dose, and the schedule of use.</span><span> </span><span>On carrying out the interview on pharmacotherapy with the parents or guardians who accompany the patient, 91.3% of the pediatric patients were not administered pharmacological treatment. It was observed that there is no adequate adherence to the pharmacological treatment in pediatric patients</span><span>.</span> 展开更多
关键词 Tooth Pain Analgesics Antimicrobial Therapy
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文章速递Antibiotic Resistance Phenotypes of <i>Enterobacteriaceae</i>Isolated from Household Wastewater in Brazzaville, Republic of Congo 认领
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作者 Rachel Moyen Tarcisse Baloki Ngoulou +1 位作者 Etienne Nguimbi Gabriel Ahombo 《微生物学(英文)》 2021年第1期27-36,共10页
Household wastewater is a source of pollution and can present health risks when discharged into the environment. Thus, samples of household wastewater from a few neighborhoods in Brazzaville were analyzed for microbio... Household wastewater is a source of pollution and can present health risks when discharged into the environment. Thus, samples of household wastewater from a few neighborhoods in Brazzaville were analyzed for microbiological quality. The various samples were cultured for isolation on solid media using conventional microbiological methods. The bacteria isolated were identified by the <i>Enterobacter</i> System gallery. Sensitivity tests were performed using the standard antibiotic susceptibility test by diffusion on Mueller Hinton medium. At the end of the analysis, 51 <i>Enterobacteriaceae</i> were isolated and identified. They included: 8 (15.68%) <i>Escherichia coli</i>, 8 (15.68%) <i>Salmonella spp.</i>, 8 (15.68%) <i>Shigella spp.</i>, 8 (15.68%) <i>Klebsiella spp.</i>, 5 (9.80%) <i>Enterobacter aerogenes</i>, 8 (15.68%) <i>Enterobacter cloacae</i>, 3 (5.90%) <i>Arizona spp.</i>, 3 (5.90%) <i>Proteus spp</i>. The results obtained show that the bacteria tested showed total resistance to the following antibiotics: amoxicillin, amoxicillin + clavulanic acid, cloxacillin and nalidixic acid. On the other hand, imipenem, cefuroxime, cefotaxime, cefftriazone and kanamycin were the most active antibiotics with low levels of resistance. The low resistance rates observed for imipenem, cefotaxime, cefuroxime and cefftriazone show that these antibiotics can be used for the treatment of infections caused by household wastewater bacteria. 展开更多
关键词 Antibiotic Therapy Enterobacteriaceae Sewage Households
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文章速递Theoretical Studies of Photodynamic Therapy Properties of Azopyridine <i>δ</i>-OsCl<sub>2</sub>(Azpy)<sub>2</sub>Complex as a Photosensitizer by a TDDFT Method 认领
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作者 Wawohinlin Patrice Ouattara Kafoumba Bamba +7 位作者 Affi Sopi Thomas Fatogoma Diarrassouba Lamoussa Ouattara Massapihanhoro Pierre Ouattara Kouakou Nobel N'guessan Mamadou Guy Richard Kone Charles Guillaume Kodjo Nahossé Ziao 《计算化学(英文)》 2021年第1期64-84,共21页
Photochemical reactions have an important place in photodynamic treatments. A good use of this therapeutic method requires a good mastery of the mechanisms of the reactions involved. Therefore, we have explored in thi... Photochemical reactions have an important place in photodynamic treatments. A good use of this therapeutic method requires a good mastery of the mechanisms of the reactions involved. Therefore, we have explored in this work the photosensitization mechanism of an organometallic complex of azopyridine <em>δ</em>-OsCl<sub>2</sub>(Azpy)<sub>2</sub> through a calculation with the method of Time Dependent Density Functional Theory TDDFT. First, we evaluated the effect of polar and non-polar solvents on the triplet and singlet excited states of this complex. Then secondly, we highlighted the photosensitization mechanism to understand how the complex acts over the diseased cells. These investigations have shown that the <em>δ</em>-OsCl<sub>2</sub>(Azpy)<sub>2</sub> complex is likely to develop photodynamic activity according to two mechanisms: on one hand, it can generate damage to DNA bases or target tissues indirectly through the production of singlet oxygen in water and in DMSO. On the second hand, through the production of the anionic superoxide radical <img src="Edit_a1e628d6-dcd2-41c6-bf3c-7e3cad491857.png" alt="" />in water can act directly or indirectly on these substrates. In addition, polar solvents are assumed to better carry out the photochemical reactions of this azopyridine complex of osmium. 展开更多
关键词 Time Dependent-Density Functional Theory Azopyridine Excited States Photosensitization Photodynamic Therapy Osmium
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Combination of epidural electrical stimulation with ex vivo triple gene therapy for spinal cord injury:a proof of principle study 认领
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作者 Filip Olegovich Fadeev Farid Vagizovich Bashirov +9 位作者 Vahe Arshaluysovich Markosyan Andrey Alexandrovich Izmailov Tatyana Vyacheslavovna Povysheva Mikhail Evgenyevich Sokolov Maxim Sergeevich Kuznetsov Anton Alexandrovich Eremeev Ilnur Ildusovich Salafutdinov Albert Anatolyevich Rizvanov Hyun Joon Lee Rustem Robertovich Islamov 《中国神经再生研究:英文版》 SCIE CAS 2021年第3期550-560,共11页
Despite emerging contemporary biotechnological methods such as gene-and stem cell-based therapy,there are no clinically established therapeutic strategies for neural regeneration after spinal cord injury.Our previous ... Despite emerging contemporary biotechnological methods such as gene-and stem cell-based therapy,there are no clinically established therapeutic strategies for neural regeneration after spinal cord injury.Our previous studies have demonstrated that transplantation of genetically engineered human umbilical cord blood mononuclear cells producing three recombinant therapeutic molecules,including vascular endothelial growth factor(VEGF),glial cell-line derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)can improve morpho-functional recovery of injured spinal cord in rats and mini-pigs.To investigate the efficacy of human umbilical cord blood mononuclear cells-mediated triple-gene therapy combined with epidural electrical stimulation in the treatment of spinal cord injury,in this study,rats with moderate spinal cord contusion injury were intrathecally infused with human umbilical cord blood mononuclear cells expressing recombinant genes VEGF165,GDNF,NCAM1 at 4 hours after spinal cord injury.Three days after injury,epidural stimulations were given simultaneously above the lesion site at C5(to stimulate the cervical network related to forelimb functions)and below the lesion site at L2(to activate the central pattern generators)every other day for 4 weeks.Rats subjected to the combined treatment showed a limited functional improvement of the knee joint,high preservation of muscle fiber area in tibialis anterior muscle and increased H/M ratio in gastrocnemius muscle 30 days after spinal cord injury.However,beneficial cellular outcomes such as reduced apoptosis and increased sparing of the gray and white matters,and enhanced expression of heat shock and synaptic proteins were found in rats with spinal cord injury subjected to the combined epidural electrical stimulation with gene therapy.This study presents the first proof of principle study of combination of the multisite epidural electrical stimulation with ex vivo triple gene therapy(VEGF,GDNF and NCAM)for treatment of spinal cord injury in rat mode 展开更多
关键词 adenoviral vector epidural electrical stimulation gene therapy glial cell-line derived neurotrophic factor human umbilical cord blood mononuclear cell neural cell adhesion molecule spinal cord injury vascular endothelial growth factor
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Induced pluripotent stem cell technology for spinal cord injury: a promising alternative therapy 认领
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作者 Yu Li Ping-Ping Shen Bin Wang 《中国神经再生研究:英文版》 SCIE CAS 2021年第8期1500-1509,共10页
Spinal cord injury has long been a prominent challenge in the trauma repair process. Spinal cord injury is a research hotspot by virtue of its difficulty to treat and its escalating morbidity. Furthermore, spinal cord... Spinal cord injury has long been a prominent challenge in the trauma repair process. Spinal cord injury is a research hotspot by virtue of its difficulty to treat and its escalating morbidity. Furthermore, spinal cord injury has a long period of disease progression and leads to complications that exert a lot of mental and economic pressure on patients. There are currently a large number of therapeutic strategies for treating spinal cord injury, which range from pharmacological and surgical methods to cell therapy and rehabilitation training. All of these strategies have positive effects in the course of spinal cord injury treatment. This review mainly discusses the problems regarding stem cell therapy for spinal cord injury, including the characteristics and action modes of all relevant cell types. Induced pluripotent stem cells, which represent a special kind of stem cell population, have gained impetus in cell therapy development because of a range of advantages. Induced pluripotent stem cells can be developed into the precursor cells of each neural cell type at the site of spinal cord injury, and have great potential for application in spinal cord injury therapy. 展开更多
关键词 axon regeneration cell therapy functional recovery induced pluripotent stem cell mesenchymal stem cell neural cells neural precursor cell neural stem cell REMYELINATION spinal cord injury stem cells
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Gene-modified leucoconcentrate for personalized ex vivo gene therapy in a mini pig model of moderate spinal cord injury 认领
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作者 Rustem R.Islamov Farid V.Bashirov +11 位作者 Mikhail E.Sokolov Andrei A.Izmailov Filip O.Fadeev Vage A.Markosyan Maria A.Davleeva Olga V.Zubkova Maxim M.Smarov Denis Yu.Logunov Boris S.Naroditskyi Ilnur I.Salafutdinov Albert A.Rizvanov Ramil G.Turaev 《中国神经再生研究:英文版》 SCIE CAS 2021年第2期357-361,共5页
We previously demonstrated that gene-modified umbilical cord blood mononuclear cells overexpressing a combination of recombinant neurotrophic factors are a promising therapeutic approach for cell-mediated gene therapy... We previously demonstrated that gene-modified umbilical cord blood mononuclear cells overexpressing a combination of recombinant neurotrophic factors are a promising therapeutic approach for cell-mediated gene therapy for neurodegenerative diseases,neurotrauma,and stroke.In this study,using a mini pig model of spinal cord injury,we proposed for the first time the use of gene-modified leucoconcentrate prepared from peripheral blood in the plastic blood bag for personalized ex vivo gene therapy.Leucoconcentrate obtained from mini pig peripheral blood was transduced with a chimeric adenoviral vector(Ad5/35 F)that carried an enhanced green fluorescent protein(EGFP)reporter gene in the plastic blood bag.The day after blood donation,the mini pigs were subjected to moderate SCI and four hours post-surgery they were intravenously autoinfused with gene-modified leucoconcentrate.A week after gene-modified leucoconcentrate therapy,fluorescent microscopy revealed EGFP-expressing leucocytes in spinal cord at the site of contusion injury.In the spleen the groups of EGFP-positive cells located in the lymphoid follicles were observed.In vitro flow cytometry and fluorescent microscopy studies of the gene-modified leucoconcentrate samples also confirmed the production of EGFP by leucocytes.Thus,the efficacy of leucocytes transduction in the plastic blood bag and their migratory potential suggest their use for temporary production of recombinant biologically active molecules to correct certain pathological conditions.This paper presents a proof-of-concept of simple,safe and effective approach for personalized ex vivo gene therapy based on gene-modified leucoconcentrate autoinfusion.The animal protocols were approved by the Kazan State Medical University Animal Care and Use Committee(approval No.5)on May 27,2014. 展开更多
关键词 chimeric Ad5/35F virus enhanced green fluorescent protein gene-modified leucoconcentrate mini pig peripheral blood personalized ex vivo gene therapy plastic blood bag spinal cord injury
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Combined administration of platelet rich plasma and autologous bone marrow aspirate concentrate for spinal cord injury: a descriptive case series 认领
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作者 Joseph A.Shehadi Steven M.Elzein +2 位作者 Paul Beery M.Chance Spalding Michelle Pershing 《中国神经再生研究:英文版》 SCIE CAS 2021年第2期362-366,共5页
Administration of platelet rich plasma(PRP) and bone marrow aspirate concentrate(BMAC) has shown some promise in the treatment of neurological conditions;however, there is limited information on combined administratio... Administration of platelet rich plasma(PRP) and bone marrow aspirate concentrate(BMAC) has shown some promise in the treatment of neurological conditions;however, there is limited information on combined administration. As such, the purpose of this study was to assess safety and functional outcomes for patients administered combined autologous PRP and BMAC for spinal cord injury(SCI). This retrospective case series included seven patients who received combined treatment of autologous PRP and BMAC via intravenous and intrathecal administration as salvage therapy for SCI. Patients were reviewed for adverse reactions and clinical outcomes using the Oswestry Disability Index(ODI) for up to 1 year, as permitted by availability of follow-up data. Injury levels ranged from C3 through T11, and elapsed time between injury and salvage therapy ranged from 2.4 months to 6.2 years. Post-procedure complications were mild and rare, consisting only of self-limited headache and subjective memory impairment in one patient. Four patients experienced severe disability prior to PRP combined with BMAC injection, as evidenced by high(> 48/100) Oswestry Disability Index scores. Longitudinal Oswestry Disability Index scores for two patients with incomplete SCI at C6 and C7, both of whom had cervical spine injuries, demonstrated a decrease of 28–40% following salvage therapy, representing an improvement from severe to minimal disability. In conclusion, intrathecal/intravenous co-administration of PRP and BMAC resulted in no significant complications and may have had some clinical benefits. Larger clinical studies are needed to further test this method of treatment for patients with SCI who otherwise have limited meaningful treatment options. This study was reviewed and approved by the Ohio Health Institutional Review Board(IRB No. 1204946) on May 16, 2018. 展开更多
关键词 bone marrow aspirate concentrate cell-based therapy neural regeneration Oswestry Disability Index platelet rich plasma spinal cord injury stem cells
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Galvanic replacement reaction for in situ fabrication of litchi-shaped heterogeneous liquid metal-Au nano-composite for radio-photothermal cancer therapy 认领
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作者 Zhenhu Guo Jingsong Lu +9 位作者 Dan Wang Wensheng Xie Yongjie Chi Jianzhong Xu Nonaka Takuya Junxin Zhang Wanling Xu Fei Gao Hong Wu Lingyun Zhao 《生物活性材料》 SCIE 2021年第3期602-612,共11页
With tremendous research advances in biomedical application,liquid metals(LM)also offer fantastic chemistry for synthesis of novel nano-composites.Herein,as a pioneering trial,litchi-shaped heterogeneous eutectic gall... With tremendous research advances in biomedical application,liquid metals(LM)also offer fantastic chemistry for synthesis of novel nano-composites.Herein,as a pioneering trial,litchi-shaped heterogeneous eutectic gallium indium-Au nanoparticles(EGaIn-Au NPs),served as effective radiosensitizer and photothermal agent for radio-photothermal cancer therapy,have been successfully prepared using in situ interfacial galvanic replacement reaction.The enhanced photothermal conversion efficiency and boosted radio-sensitization effect could be achieved with the reduction of Au nanodots onto the eutectic gallium indium(EGaIn)NPs surface.Most importantly,the growth of tumor could be effectively inhibited under the combined radio-photothermal therapy mediated by EGaIn-Au NPs.Inspired by this approach,in situ interfacial galvanic replacement reaction may open a novel strategy to fabricate LM-based nano-composite with advanced multi-functionalities. 展开更多
关键词 Au nanodots Gallium Galvanic replacement reaction Indium Radio-photothermal therapy
Redox responsive nanoparticle encapsulating black phosphorus quantum dots for cancer theranostics 认领
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作者 Haolin Chen Zhiming Liu +7 位作者 Bo Wei Jun Huang Xinru You Jingyang Zhang Zhiling Yuan Zhilie Tang Zhouyi Guo Jun Wu 《生物活性材料》 SCIE 2021年第3期655-665,共11页
Effective cancer treatment puts high demands for cancer theranostics.For cancer diagnostics,optical coherence tomography(OCT)technology(including photothermal optical coherence tomography(PT-OCT))has been widely inves... Effective cancer treatment puts high demands for cancer theranostics.For cancer diagnostics,optical coherence tomography(OCT)technology(including photothermal optical coherence tomography(PT-OCT))has been widely investigated since it induces changes in optical phase transitions in tissue through environmental changes(such as temperature change for PT-OCT).In this report,redox responsive nanoparticle encapsulating black phosphorus quantum dots was developed as a robust PT-OCT agent.Briefly,black phosphorus quantum dots(BPQDs)are incorporated into cysteine-based poly-(disulfide amide)(Cys-PDSA)to form stable and biodegradable nanoagent.The excellent photothermal feature allows BPQD/Cys-PDSA nanoparticles(NPs)as a novel contrast agent for high-resolution PT-OCT bioimaging.The Cys-PDSA can rapidly respond to glutathione and effectively release BPQDs and drugs in vitro and in vivo.And the obtained NPs exhibit excellent near-infrared(NIR)photothermal transduction efficiency and drug delivery capacity that can serve as novel therapeutic platform,with very low chemo drug dosage and side effects.Both of the polymer and BPQD are degradable,indicating this platform is a rare PT-OCT agent that is completely biodegradable.Overall,our research highlights a biodegradable and biocompatible black phosphorus-based nanoagent for both cancer diagnosis and therapy. 展开更多
关键词 Optical coherence tomography Black phosphorus THERANOSTIC PHOTOTHERMAL Cancer therapy
Role of argon plasma coagulation in treatment of esophageal varices 认领
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作者 Ying Song Yuan Feng +6 位作者 Li-Hui Sun Bo-Jiang Zhang Hong-Juan Yao Jing-Gui Qiao Shu-Fen Zhang Ping Zhang Bin Liu 《世界临床病例杂志》 SCIE 2021年第3期521-527,共7页
With the development of endoscopic therapy,argon plasma coagulation(APC)has been widely used by endoscopists.It has many advantages,such as simple to operate,low cost,and minimal invasiveness.Because of its capability... With the development of endoscopic therapy,argon plasma coagulation(APC)has been widely used by endoscopists.It has many advantages,such as simple to operate,low cost,and minimal invasiveness.Because of its capability of lesion ablation and hemostasis,APC has several indications in the gastrointestinal tract.One of them is esophageal varices.The aim of this review is to summarize the research on APC in this field to provide a reference for clinical practice. 展开更多
关键词 Esophageal varices Argon plasma coagulation Clinical practice Endoscopic therapy Gastrointestinal tract Minimally invasive
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