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诱导多能干细胞体外分化为雄性生殖细胞的研究进展 认领
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作者 毋姗姗 范文娟 +1 位作者 李瑞玲 邓锦波 《中华生殖与避孕杂志》 北大核心 2021年第1期89-94,共6页
男性不育是影响生殖健康的重要问题,由于致病因素和发病机制复杂,临床上针对非梗阻性无精子症等不明原因的男性不育症往往缺乏特异性治疗措施。随着生殖细胞体外诱导培养的技术的发展,研究者们希望利用无精子症患者源性的多能干细胞体... 男性不育是影响生殖健康的重要问题,由于致病因素和发病机制复杂,临床上针对非梗阻性无精子症等不明原因的男性不育症往往缺乏特异性治疗措施。随着生殖细胞体外诱导培养的技术的发展,研究者们希望利用无精子症患者源性的多能干细胞体外培养出自身具有功能性的精子,从而治疗男性不育症。体外培养生殖细胞的策略,通常是先将诱导多能干细胞分化为原始生殖样细胞,然后进一步诱导分化为单倍体精子样细胞,其培养的难点在于将二倍体生殖细胞减数分裂为单倍体的精细胞,但是目前这个问题还没有得到解决。我们对诱导多能干细胞诱导雄性配子的技术进展和原理进行综述,希望能帮助我们更好地理解体外精子发生的机制,为体外受精的临床应用提供参考。 展开更多
关键词 多能干细胞 雄性生殖细胞 精子发生 诱导分化
基于生物信息学方法的小鼠精子发生关键基因筛选 认领
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作者 李欣 田佳 +6 位作者 脱征军 邵怀峰 张伟新 李委奇 王琨 徐寒 温万 《中国畜牧杂志》 CAS 北大核心 2021年第2期84-89,共6页
为探索精子发生的分子机制,寻找精子变形期间的关键基因,本研究从公共基因表达数据库下载小鼠精细胞的转录组测序数据,利用R软件Ballgown程序包筛选差异表达基因,并对差异表达基因进行Gene ontology(GO)和Kyoto Encyclopedia of Genes a... 为探索精子发生的分子机制,寻找精子变形期间的关键基因,本研究从公共基因表达数据库下载小鼠精细胞的转录组测序数据,利用R软件Ballgown程序包筛选差异表达基因,并对差异表达基因进行Gene ontology(GO)和Kyoto Encyclopedia of Genes and Genomes(KEGG)富集分析后,再对筛选出的关键基因进行分析。结果显示:筛选出8个相关性较强的关键通路,将其所包含的基因进行FPKM值从大到小排序,取前18名的基因进行功能分析。其中,Txnrd3和Tcp1与繁殖活动相关;Gpx4、Dkkl1、Dbil5与发育相关;Prm2、Selenof、Tnp1、Tnp2、Ropn1l、Pafah1b2、Spink2和Prm1等8个基因参与精子发生;Spa17、Ldhc、Hsp90aa1、Gstm5和Csnk2b参与鞭毛和纤毛的形成。以上基因对精子发生具有重要作用,深入发掘这些基因的功能特点可为进一步揭示精子变形机制提供理论依据。 展开更多
关键词 精子发生 生物信息学分析 差异基因
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人工驯养树鼩精子发生过程中MCM7蛋白的表达 认领
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作者 何光勇 唐健 +3 位作者 杨芳 钱忠义 杨榆玲 罗兰 《昆明医科大学学报》 CAS 2021年第1期1-5,共5页
目的探讨MCM7蛋白在人工驯养树鼩精子发生过程中的表达特点及其表达是否存在季节性差异,为以树鼩作为动物模型进行生殖研究提供基础性实验数据。方法对全年12组不同月份的成年人工驯养树鼩睾丸组织进行HE染色及MCM7蛋白的免疫组化检测... 目的探讨MCM7蛋白在人工驯养树鼩精子发生过程中的表达特点及其表达是否存在季节性差异,为以树鼩作为动物模型进行生殖研究提供基础性实验数据。方法对全年12组不同月份的成年人工驯养树鼩睾丸组织进行HE染色及MCM7蛋白的免疫组化检测。结果 1~12月份人工驯养树鼩睾丸组织中,MCM7蛋白在曲精小管中主要表达于精原细胞和部分初级精母细胞,其阳性细胞率在2月份和3月份较全年其他月份均有显著性差异(P <0.05)。结论人工驯养树鼩精子发生过程中,MCM7蛋白可能在精原细胞和初级精母细胞的DNA复制过程中发挥作用,其蛋白表达的活跃程度存在季节性差异。 展开更多
关键词 人工驯养树鼩 精子发生 MCM7蛋白
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白消安处理消融猪内源精原干细胞的效果及外源精原干细胞移植 认领
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作者 赵鑫 邢萍萍 +2 位作者 张恒 杨化强 吴珍芳 《华南农业大学学报》 CAS 北大核心 2021年第1期1-11,共11页
【目的】研究猪睾丸组织注射白消安消融猪内源精原干细胞(Spermatogonial stem cell,SSC)的效果,以及猪SSC同种异体移植后对内源性SSC消融受体生殖能力恢复的影响。【方法】采用3 mg/kg剂量的白消安对9头6周龄大白公猪进行睾丸注射,另外... 【目的】研究猪睾丸组织注射白消安消融猪内源精原干细胞(Spermatogonial stem cell,SSC)的效果,以及猪SSC同种异体移植后对内源性SSC消融受体生殖能力恢复的影响。【方法】采用3 mg/kg剂量的白消安对9头6周龄大白公猪进行睾丸注射,另外3头注射2 mL二甲基亚砜作为对照。3周后,对试验组公猪以相同剂量进行第2次睾丸注射。第2次注射3周后,采集试验组和对照组公猪睾丸进行相关检测,评估内源性SSC消融情况。第2次白消安注射1个月后,以两步酶消化法处理5~7日龄大白仔猪睾丸分离得到睾丸单细胞悬液,并用明胶差速贴壁法进行纯化,纯化后以免疫荧光和流式细胞术分析SSC的纯度。异体移植SSC 4个月后,用微卫星标记检测受体公猪精液以及睾丸组织中供体来源SSC的存在。【结果】以3 mg/kg剂量的白消安处理大白公猪2次后,睾丸组织苏木精−伊红染色以及免疫组织化学染色结果显示,试验组睾丸曲细精管中各级生精细胞消融但其支持细胞结构完好,可以支持外源性SSC的定植及发育。免疫荧光以及流式细胞术结果表明,分离得到的睾丸单细胞悬液经纯化后UCHL-1阳性细胞占比由差速贴壁前的16.3%提高到了50.8%。苏木精−伊红染色以及免疫组织化学染色结果显示,猪SSC移植4个月后,移植组睾丸组织生精细胞恢复,在受体睾丸曲细精管基底膜上可检测到UCHL-1阳性SSC。受体睾丸组织的微卫星标记分析显示了供体SSC的存在,表明移植进入受体睾丸中的供体SSC可以在受体睾丸中定植存活超过4个月;精液微卫星标记未检测到供体来源的精子。【结论】以3 mg/kg剂量的白消安注射公猪睾丸能有效消融内源性SSC,可以用来制备SSC移植的受体猪。两步酶消化及明胶差速贴壁法可成功分离纯化猪SSC。猪SSC经同种异体移植后可以在受体睾丸中定植存活超过4个月。 展开更多
关键词 猪 精原干细胞 白消安 细胞移植 精子发生
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SP1被鉴定为miR-122-5p的一个转录因子 认领
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作者 沈建云 朱茂英 +1 位作者 费路敏 陈德宇 《中华男科学杂志》 CAS 北大核心 2021年第1期11-16,共6页
目的:miR-122-5p跟精子发生相关,本研究主要是探究miR-122-5p在睾丸内的转录因子。方法:采用生物信息学方法预测到miR-122-5p启动子的可能转录因子为SP1和GATA4,然后分别构建双荧光素酶pGL3-miR-122-5p promoter载体、pcDNA3.1(+)-SP1... 目的:miR-122-5p跟精子发生相关,本研究主要是探究miR-122-5p在睾丸内的转录因子。方法:采用生物信息学方法预测到miR-122-5p启动子的可能转录因子为SP1和GATA4,然后分别构建双荧光素酶pGL3-miR-122-5p promoter载体、pcDNA3.1(+)-SP1表达载体和pcDNA3.1(+)-GATA4表达载体,再将pcDNA-SP1+pGL3-basic混合质粒和pcDNA-SP1+pGL3-miR-122-5p promoter混合质粒,以及pcDNA-GATA4+pGL3-basic混合质粒和pcDNA-GATA4+pGL3-miR-122-5p promoter混合质粒分别转入293T细胞,最后用双荧光素酶检测系统检测酶活性。结果:pcDNA3.1+pGL3-miR-122 promoter组的荧光值为0.0362±0.0004,显著高于pcDNA3.1+pGL3-basic组(P<0.05),表明已成功构建小鼠miR-122-5p启动子荧光素酶报告质粒。pcDNA-SP1+pGL3-miR-122-5p promoter组荧光值显著高于pcDNA-SP1+pGL3-basic(P<0.05),结果提示转录因子SP1能够促进miR-122的转录。pcDNA-GATA4+pGL3-basic混合质粒转染组的荧光值与pcDNA-GATA4+pGL3-miR-122-5p promoter混合质粒转染组之间差异不显著,表明GATA4不能增加miR-122-5p转录能力。结论:转录因子SP1能够促进miR-122-5p的转录,GATA4对miR-122-5p没有转录增强能力。 展开更多
关键词 miR-122-5p SP1 转录因子 精子发生
The testis-specifically expressed gene Trim69 is not essential for fertility in mice 认领
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作者 Xi He Wenxiu Xie +4 位作者 Huiling Li Yiqiang Cui Ya Wang Xuejiang Guo Jiahao Sha 《生物医学研究杂志:英文版》 CAS 2021年第1期47-60,共14页
Protein ubiquitination is essential for diverse cellular functions including spermatogenesis.The tripartite motif(TRIM)family proteins,most of which have E3 ubiquitin ligase activity,are highly conserved in mammals.Th... Protein ubiquitination is essential for diverse cellular functions including spermatogenesis.The tripartite motif(TRIM)family proteins,most of which have E3 ubiquitin ligase activity,are highly conserved in mammals.They are involved in important cellular processes such as embryonic development,immunity,and fertility.Our previous studies indicated that Trim69,a testis-specific expressed TRIM family gene,potentially participates in the spermatogenesis by mediating testicular cells apoptosis.In this study,we investigated the biological functions of Trim69 in male mice by established Trim69 knockout mice with CRISPR/Cas9 genomic editing technology.Here,we reported that the male Trim69 knockout mice had normal fertility.The adult knockout mice have shown that the appearance of testes,testis/body weight ratios,testicular histomorphology,and the number and quality of sperm were consistent with wild-type mice.These results indicated that the E3 ubiquitin ligase protein Trim69 was not essential for male mouse fertility,and it might be compensated by other TRIM family members such as Trim58 in Trim69-deficiency testis.This study would help to elucidate the functions of tripartite motif protein family and the regulation of spermatogenesis. 展开更多
关键词 Trim69 gene knockout SPERMATOGENESIS E3 ubiquitin ligase male fertility
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雄性生殖系统中瘦素表达及功能的研究进展 认领
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作者 金明昊 黄文一 +3 位作者 张梦旖 张一苇 刘悦 丁之德 《国际生殖健康/计划生育杂志》 CAS 2021年第1期38-43,共6页
男性生育力呈下降趋势,阐明其发生机制有助于男性不育症的精准医疗。瘦素是一种主要由脂肪组织产生的激素,在调节机体能量代谢、参与炎性反应、促进生殖系统发育及维持其正常功能等方面具有重要的作用。瘦素及其受体在哺乳动物下丘脑-垂... 男性生育力呈下降趋势,阐明其发生机制有助于男性不育症的精准医疗。瘦素是一种主要由脂肪组织产生的激素,在调节机体能量代谢、参与炎性反应、促进生殖系统发育及维持其正常功能等方面具有重要的作用。瘦素及其受体在哺乳动物下丘脑-垂体-性腺(HPG)轴的内分泌器官、睾丸、生殖道、附属生殖腺以及精子中均有广泛表达,其表达异常与雄性生殖系统发育迟缓或功能障碍相关。阐明瘦素在雄性生殖系统中的表达及作用机制可为临床上治疗男性不育等相关疾病提供重要的理论基础。 展开更多
关键词 瘦素 受体 瘦素 泌尿生殖系统 生育力 精子发生 下丘脑-垂体-性腺轴
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文章速递NC1-peptide derived from collagenα3(IV)chain is a blood-tissue barrier regulator:lesson from the testis 认领
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作者 Shi-Wen Liu Hui-Tao Li +1 位作者 Ren-Shan Ge C Yan Cheng 《亚洲男性学杂志:英文版》 SCIE CAS CSCD 2021年第2期123-128,共6页
Collagenα3(IV)chains are one of the major constituent components of the basement membrane in the mammalian testis.Studies have shown that biologically active fragments,such as noncollagenase domain(NC1)-peptide,can b... Collagenα3(IV)chains are one of the major constituent components of the basement membrane in the mammalian testis.Studies have shown that biologically active fragments,such as noncollagenase domain(NC1)-peptide,can be released from the C-terminal region of collagenα3(IV)chains,possibly through the proteolytic action of metalloproteinase 9(MMP9).NC1-peptide was shown to promote blood-testis barrier(BTB)remodeling and fully developed spermatid(e.g.,sperm)release from the seminiferous epithelium because this bioactive peptide was capable of perturbing the organization of both actin-and microtubule(MT)-based cytoskeletons at the Sertoli cell-cell and also Sertoli-spermatid interface,the ultrastructure known as the basal ectoplasmic specialization(ES)and apical ES,respectively.More importantly,recent studies have shown that this NC1-peptide-induced effects on cytoskeletal organization in the testis are mediated through an activation of mammalian target of rapamycin complex 1/ribosomal protein S6/transforming retrovirus Akt1/2 protein(mTORC1/rpS6/Akt1/2)signaling cascade,involving an activation of cell division control protein 42 homolog(Cdc42)GTPase,but not Ras homolog family member A GTPase(RhoA),and the participation of end-binding protein 1(EB1),a microtubule plus(+)end tracking protein(+TIP),downstream.Herein,we critically evaluate these findings,providing a critical discussion by which the basement membrane modulates spermatogenesis through one of its locally generated regulatory peptides in the testis. 展开更多
关键词 collagenα3(IV)chain F-actin microtubules noncollagenase domain(NC1)-peptide spermatogenesis testis
精子发生研究进展 认领
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作者 朱文倩 蔡宁宁 +2 位作者 杨蕊 石建忠 张学明 《生命科学》 CSCD 北大核心 2020年第10期1021-1028,共8页
精子发生(spermatogenesis)是遗传信息传递和物种延续的重要环节,受诸多因素的调控。精子发生作为组织胚胎学、分子生物学、遗传学等多学科的交叉领域,是生殖生物学的研究热点之一。本文评述了精子发生相关研究的最新进展,介绍了生精细... 精子发生(spermatogenesis)是遗传信息传递和物种延续的重要环节,受诸多因素的调控。精子发生作为组织胚胎学、分子生物学、遗传学等多学科的交叉领域,是生殖生物学的研究热点之一。本文评述了精子发生相关研究的最新进展,介绍了生精细胞的分子调控、睾丸细胞对精子发生的影响以及精子发生重建技术的应用,以期为探索精子发生机制提供新的思路。 展开更多
关键词 精子发生 睾丸体细胞 精子发生重建 表观遗传
A Qualitative Model of the Interaction of Sexual Behavior and Hormone Gene Transcription in Male Blue Gourami during Reproduction 认领
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作者 Gad Degani 《生物物理化学(英文)》 2020年第4期43-50,共8页
In the present study, a model is suggested to describe hormone control in male blue gourami (<i><span style="font-family:Verdana;">Trichogaster trichopterus</span></i><span style=&... In the present study, a model is suggested to describe hormone control in male blue gourami (<i><span style="font-family:Verdana;">Trichogaster trichopterus</span></i><span style="font-family:Verdana;">) along the gonadotropic brain</span><span style="font-size:10pt;font-family:Verdana;">-</span><span style="font-size:10pt;font-family:Verdana;">pituitary</span><span style="font-size:10pt;font-family:Verdana;">- </span><span style="font-size:10pt;font-family:Verdana;">gonad axis (BPG axis) and the hypothalamic-pituitary-somatotropic axis (HPS axis). This model is based on the cloning</span><span style="font-size:10.0pt;font-family:""><span style="font-family:Verdana;"> and transcription of genes encoding hormones of the two axes involved in spermatogenesis during blue gourami reproduction. Gene transcription is affected by environmental, biological, </span><span style="font-family:Verdana;">and behavioral factors. Mature males were examined in two different stages—nonreproductive in high-density habitats and reproductive in low-density </span><span style="font-family:Verdana;">habitats. Based on gene transcription, gonadotropin-releasing hormone 1 (GnRH1) was involved in controlling spermatogenesis (spermatogonia to spermatids) via the BPG axis in nonreproductive and reproductive stages by controlling follicle-stimulating hormone (FSH), 11-ketotestosterone (11KT) and 17</span><i><span style="font-family:Verdana;">β</span></i><span style="font-family:Verdana;">-estradiol (E</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;">). However, GnRH3 had a larger effect during the reproductive stage via the BPG axis (spermatids to sperm) on luteinizing hormone (LH), 11KT, and 17</span><i><span style="font-family:Verdana;">α</span></i></span><span style="font-size:10pt;font-family:Verdana;">-</span><span style="font-size:10pt;font-family:Verdana;">hydroxyprogesterone (17P). At the same time, the HPS axis was involved in spermatogenesis via pituitary adenylate cyclase-activating 展开更多
关键词 Qualitative Model Anabantidae HORMONE Gene Trichogaster SPERMATOGENESIS SPERM GNRH 11-Ketotestosterone Gonadotropic Brain Pituitary Gonad Axis
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Gonadotropin treatment for male partial congenital hypogonadotropic hypogonadism in Chinese patients 认领
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作者 Ming Hao Min Nie +7 位作者 Bing-Qing Yu Yin-Jie Gao Xi Wang Wan-Lu Ma Qi-Bin Huang Rui Zhang Jiang-Feng Mao Xue-Yan Wu 《亚洲男性学杂志:英文版》 SCIE CAS CSCD 2020年第4期390-395,共6页
Partial congenital hypogonadotropic hypogonadism(PCHH)is caused by an insufficiency in,but not a complete lack of,gonadotropin secretion.This leads to reduced testosterone production,mild testicular enlargement,and pa... Partial congenital hypogonadotropic hypogonadism(PCHH)is caused by an insufficiency in,but not a complete lack of,gonadotropin secretion.This leads to reduced testosterone production,mild testicular enlargement,and partial pubertal development.No studies have shown the productivity of spermatogenesis in patients with PCHH.We compared the outcomes of gonadotropin-induced spermatogenesis between patients with PCHH and those with complete congenital hypogonadotropic hypogonadism(CCHH).This retrospective study included 587 patients with CHH who were treated in Peking Union Medical College Hospital(Beijing,China)from January 2008 to September 2016.A total of 465 cases were excluded from data analysis for testosterone or gonadotropin-releasing hormone treatment,cryptorchidism,poor compliance,or incomplete medical data.We defined male patients with PCHH as those with a testicular volume of≥4 ml and patients with a testicular volume of<4 ml as CCHH.A total of 122 compliant,noncryptorchid patients with PCHH or CCHH received combined human chorionic gonadotropin and human menopausal gonadotropin and were monitored for 24 months.Testicular size,serum luteinizing hormone levels,follicle-stimulating hormone levels,serum total testosterone levels,and sperm count were recorded at each visit.After gonadotropin therapy,patients with PCHH had a higher spermatogenesis rate(92.3%)than did patients with CCHH(74.7%).During 24-month combined gonadotropin treatment,the PCHH group took significantly less time to begin producing sperm compared with the CCHH group(median time:11.7 vs 17.8 months,P<0.05).In conclusion,after combined gonadotropin treatment,patients with PCHH have a higher spermatogenesis success rate and sperm concentrations and require shorter treatment periods for sperm production. 展开更多
关键词 gonadotropin treatment partial congenital hypogonadotropic hypogonadism SPERMATOGENESIS
Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2)depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis 认领
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作者 Bin Lei Li-Xia Xie +5 位作者 Shou-Bo Zhang Bo Wan Li-Ren Zhong Xu-Ming Zhou Xiang-Ming Mao Fang-Peng Shu 《亚洲男性学杂志:英文版》 SCIE CAS CSCD 2020年第5期493-499,共7页
Phosphoribosyl-pyrophosphate synthetase 2(PRPS2)is a rate-limiting enzyme and plays an important role in purine and pyrimidine nucleotide synthesis.Recent studies report that PRPS2 is involved in male infertility.Howe... Phosphoribosyl-pyrophosphate synthetase 2(PRPS2)is a rate-limiting enzyme and plays an important role in purine and pyrimidine nucleotide synthesis.Recent studies report that PRPS2 is involved in male infertility.However,the role of PRPS2 in hypospermatogenesis is unknown.In this study,the relationship of PRPS2 with hypospermatogenesis and spermatogenic cell apoptosis was investigated.The results showed that PRPS2 depletion increased the number of apoptotic spermatogenic cells in vitro.PRPS2 was downregulated in a mouse model of hypospermatogenesis.When PRPS2 expression was knocked down in mouse testes,hypospermatogenesis and accelerated apoptosis of spermatogenic cells were noted.E2F transcription factor 1(E2F1)was confirmed as the target gene of PRPS2 and played a key role in cell apoptosis by regulating the P53/Bcl-xl/Bcl-2/Caspase 6/Caspase 9 apoptosis pathway.Therefore,these data indicate that PRPS2 depletion contributes to the apoptosis of spermatogenic cells and is associated with hypospermatogenesis,which may be helpful for the diagnosis of male infertility. 展开更多
关键词 hypospermatogenesis male infertility molecular marker phosphoribosyl-pyrophosphate synthetase 2 SPERMATOGENESIS
十一酸睾酮原位凝胶植入剂避孕效果及对雄性大鼠生殖系统的影响 认领
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作者 张晓伟 金瑛 +4 位作者 易东旭 杨立群 张翀 刘丹华 孟胜男 《医药导报》 CAS 北大核心 2020年第11期1496-1501,共6页
目的制备十一酸睾酮原位凝胶植入剂(TU-ISFI),考察其在大鼠体内避孕效果及对雄性大鼠生殖系统的影响。方法以聚己内酯丙交酯为凝胶骨架材料,N-甲基吡咯烷酮为有机溶剂,制备TU-ISFI。以市售十一酸睾酮注射液为阳性对照,大鼠皮下注射植入T... 目的制备十一酸睾酮原位凝胶植入剂(TU-ISFI),考察其在大鼠体内避孕效果及对雄性大鼠生殖系统的影响。方法以聚己内酯丙交酯为凝胶骨架材料,N-甲基吡咯烷酮为有机溶剂,制备TU-ISFI。以市售十一酸睾酮注射液为阳性对照,大鼠皮下注射植入TU-ISFI,考察大鼠血浆药物浓度、精子计数、精子活动力、生殖能力和睾丸、附睾等生殖器官变化。结果体内外药物释放结果表明,TU-ISFI 3个月内能够稳定释放,大鼠体内血药浓度平稳,无明显波谷现象。皮下植入1个月后,TU-ISFI各剂量组大鼠附睾精子计数和精子活动力均明显下降。给药3个月后,TU-ISFI大、中剂量组(600,300 mg)生殖配对,大鼠完全丧失生殖能力,TU-ISFI小剂量组(150 mg)部分大鼠保留繁殖能力,子代个数明显低于对照组。结论TU-ISFI具有平稳药物缓控释能力,对雄性大鼠避孕效果优良,是一种具有广阔应用前景的激素补充制剂和男性避孕制剂。 展开更多
关键词 十一酸睾酮 原位凝胶植入剂 避孕 精子发生 药物缓释
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睾丸优势表达的Boule蛋白影响果蝇发育 认领
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作者 夏文娟 肖天衡 +1 位作者 黄娟 徐宇君 《中国科学:生命科学》 CSCD 北大核心 2020年第4期438-445,共8页
boule基因是屈指可数的高度保守、横跨后生动物的调控多个物种精子发生所不可或缺的基因.在果蝇中,boule基因突变体bol1可导致雄性不育,而boule基因的大片段缺失突变体boule40却表现了致死表型,提示boule可能在胚胎发育过程中也起到一... boule基因是屈指可数的高度保守、横跨后生动物的调控多个物种精子发生所不可或缺的基因.在果蝇中,boule基因突变体bol1可导致雄性不育,而boule基因的大片段缺失突变体boule40却表现了致死表型,提示boule可能在胚胎发育过程中也起到一定的作用.为了验证这一假设,首先,本团队利用RT-PCR检测了boule基因在不同组织中的表达情况,并构建了GFP敲入突变体检测Boule蛋白在不同组织中的分布,结果表明boule mRNA及Boule蛋白在发育过程及成蝇的头、胸、腹中均存在.其次,构建了boule基因完全缺失突变体,发现在不同发育阶段均有致死发生.为了探寻这种影响是来自boule基因产生的蛋白还是非编码RNA或是内含子产生的未发现的基因,本团队又构建了boule基因单碱基缺失的移码突变体,发现同样有致死现象.最后,全身性过表达Boule蛋白也可导致果蝇发育过程中的逐渐死亡.以上结果都说明,果蝇体细胞中的Boule蛋白在发育过程中起重要作用,并需要进行精确调控.这一睾丸以外的作用目前只在昆虫中发现. 展开更多
关键词 boule 果蝇 睾丸 精子发生 体细胞 发育
PCB118暴露对青春期雄性ICR小鼠精子发生的影响及机制 认领
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作者 沈文敏 陶钰荣 +3 位作者 张琳 孟令娇 刘丽 刘树真 《中国药理学与毒理学杂志》 CAS 北大核心 2020年第11期839-846,共8页
目的观察青春期雄性ICR小鼠暴露PCB118对精子发生的损害作用,探究其与睾酮合成过程相关的作用机制。方法36只4周龄雄性ICR小鼠随机分为3组,每组12只,每日ig给予PCB118(50和500μg·kg^(-1))或溶媒(玉米油),连续4周。给药结束后计算... 目的观察青春期雄性ICR小鼠暴露PCB118对精子发生的损害作用,探究其与睾酮合成过程相关的作用机制。方法36只4周龄雄性ICR小鼠随机分为3组,每组12只,每日ig给予PCB118(50和500μg·kg^(-1))或溶媒(玉米油),连续4周。给药结束后计算脏器系数;HE染色观察睾丸组织病理变化,统计曲细精管直径、生精上皮厚度和精原细胞相对数目;检测附睾精子数目、精子活力和精子畸形率;用放射免疫法检测血清睾酮水平;RT-qPCR法检测睾丸中与睾酮合成相关的酶和雄激素受体(Ar)mRNA水平;Western印迹法和免疫组织化学法检测AR蛋白和增殖细胞核抗原(PCNA)蛋白的表达。结果与溶媒对照组相比,PCB118500μg·kg^(-1)组肝系数明显下降(P<0.01),PCB1182个剂量组睾丸、附睾、肾和脾系数均无显著变化。PCB1182个剂量组睾丸组织中出现精原细胞缺失、曲细精管空泡化,曲细精管直径、生精上皮厚度和精原细胞相对数目均显著下降(P<0.05,P<0.01);附睾中精子数量显著减少,畸形率显著增加(P<0.05),但精子活力无显著变化;血清睾酮水平显著下降(P<0.05);睾丸中Ar及与睾酮合成相关的酶类固醇合成急性调节酶、胆固醇侧链裂解酶、3β-羟基类固醇脱氢酶、17-20裂解酶和17β-羟基类固醇脱氢酶的mRNA表达水平显著下降(P<0.05);AR和PCNA蛋白表达显著下降(P<0.05)。结论PCB118导致生精细胞增殖抑制,精子质量下降,该作用与其降低血清睾酮水平、下调睾酮合成相关酶mRNA水平、降低AR蛋白和mRNA表达以及降低PCNA蛋白表达有关。 展开更多
关键词 2 3’ 4 4’ 5-五氯联苯 精子发生 睾酮合成 雄激素受体
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精子发生相关基因SPATA功能的研究进展 认领
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作者 张英 翟晓娜 《河南大学学报:自然科学版》 CAS 2020年第6期684-690,共7页
SPATA基因是一类与精子发生相关的基因.精子发生依赖于一系列相关基因的正常表达与调控,SPATA基因在这个过程中起着一定的作用.本文通过参考大量SPATA基因相关文献,从SPATA基因在细胞增殖、细胞凋亡中的作用以及SPATA基因对多种相关疾... SPATA基因是一类与精子发生相关的基因.精子发生依赖于一系列相关基因的正常表达与调控,SPATA基因在这个过程中起着一定的作用.本文通过参考大量SPATA基因相关文献,从SPATA基因在细胞增殖、细胞凋亡中的作用以及SPATA基因对多种相关疾病产生的影响和发病机理等多个方面,综述了精子发生过程中该类基因功能的研究进展,对于今后研究精子发生过程中有关特异基因的表达和细胞增殖、凋亡机理提供新思路;为研究精子发生的调控机制提供参考途径和方法;对于了解精子发生过程中产生的各种相关疾病的病因具有重要指导意义. 展开更多
关键词 精子发生 SPATA基因 睾丸发育 细胞增殖 细胞凋亡
孕期非那雄胺暴露致子代雄性小鼠生殖器官发育异常 认领
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作者 范霞 李飞 黄璜 《中国病理生理杂志》 CAS CSCD 北大核心 2020年第2期323-328,共6页
目的:探讨孕期非那雄胺暴露对子代雄性小鼠生殖器官发育的影响。方法:CD-1小鼠在受孕后0~17 d给予非那雄胺处理,通过宏观观察、解剖分析与组织形态学染色观察子代雄性小鼠生殖器官的发育情况;通过免疫荧光染色分析子代雄性小鼠精子发生... 目的:探讨孕期非那雄胺暴露对子代雄性小鼠生殖器官发育的影响。方法:CD-1小鼠在受孕后0~17 d给予非那雄胺处理,通过宏观观察、解剖分析与组织形态学染色观察子代雄性小鼠生殖器官的发育情况;通过免疫荧光染色分析子代雄性小鼠精子发生情况。结果:宏观观察结果显示,孕期非那雄胺暴露可导致子代雄性小鼠外生殖器官畸形,表现为阴囊未完全融合及阴茎畸形;此外,还观察到小鼠肛门与生殖器的距离显著缩短(P<0.01)。解剖分析结果显示,孕期非那雄胺暴露可导致子代雄性小鼠睾丸不同程度的不完全下降及长度显著缩短(P<0.01)。组织形态学结果显示,各阶段阴茎长度均显著缩短(P<0.01);睾丸生精小管密度和生精小管管腔成熟精子数均显著降低(P<0.01),生精小管管腔和睾丸间质间隙均显著增大(P<0.01)。免疫荧光染色结果显示,睾丸中支持细胞、睾丸间质细胞和精原细胞的密度均显著降低(P<0.01);生精小管细胞的caspase-3荧光强度显著增加(P<0.01),Ki67与沙漠刺猬因子(desert hedgehog,Dhh)荧光强度均显著降低(P<0.01)。结论:孕期非那雄胺暴露可导致子代小鼠生殖器官发育异常并影响精子发生。 展开更多
关键词 非那雄胺 孕期暴露 性发育障碍 生殖器官 精子发生
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Possible links between COVID-19 and male fertility 认领
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作者 Roland Eghoghosoa Akhigbe Moses Agbomhere Hamed 《亚太生殖杂志:英文版》 2020年第5期211-214,共4页
The coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)may have a ripple effect that puts men at a risk of infertility.This article reviews the possible lin... The coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)may have a ripple effect that puts men at a risk of infertility.This article reviews the possible link between SARS-CoV-2 infection and male reproduction following speculations that the single-stranded RNA viruses could directly invade the testes.SARS-CoV-2 enters the human lung cells via angiotensin converting enzyme 2(ACE2).ACEs,its products,angiotensin-(1-7),and its receptor,MAS receptor,are expressed in the testes.Although the binding of SAR-CoV-2 to ACE2 could lead to excess angiotensinⅡwith possible enhanced inflammation,angiotensinⅡcould also promote sperm motility.In addition,the pathophysiology of SAR-CoV-2,especially in relation to male fertility,is yet to be fully understood;the suppression of androgen observed in COVID-19 infected men calls for the need for andrological assessment in infected male. 展开更多
关键词 SARS-CoV-2 COVID-19 Angiotensin converting enzyme Steroidogenesis SPERMATOGENESIS
Recent advances in mammalian reproductive biology 认领
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作者 Lu Wen Qiang Liu +11 位作者 Jingjing Xu Xixi Liu Chaoyi Shi Zuwei Yang Yili Zhang Hong Xu Jiang Liu Hui Yang Hefeng Huang Jie Qiao Fuchou Tang Zi-Jiang Chen 《中国科学:生命科学英文版》 SCIE CAS CSCD 2020年第1期18-58,共41页
Reproductive biology is a uniquely important topic since it is about germ cells, which are central for transmitting genetic information from generation to generation. In this review, we discuss recent advances in mamm... Reproductive biology is a uniquely important topic since it is about germ cells, which are central for transmitting genetic information from generation to generation. In this review, we discuss recent advances in mammalian germ cell development,including preimplantation development, fetal germ cell development and postnatal development of oocytes and sperm. We also discuss the etiologies of female and male infertility and describe the emerging technologies for studying reproductive biology such as gene editing and single-cell technologies. 展开更多
关键词 PREIMPLANTATION fetal GERM cell OOGENESIS SPERMATOGENESIS female INFERTILITY male INFERTILITY gene editing SINGLE-CELL sequencing
Exploring the reproductive ecology of the tropical semifossorial snake Ninia atrata 认领
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作者 Teddy Angarita-Sierra Cesar Alejandro Lopez-Hurtado 《动物学研究》 SCIE CAS CSCD 2020年第2期157-171,共15页
Based on histological analyses and field studies,this research describes the reproductive ecology of a population of Ninia atrata snakes inhabiting an oil palm plantation.Furthermore,through a multivariate approach,we... Based on histological analyses and field studies,this research describes the reproductive ecology of a population of Ninia atrata snakes inhabiting an oil palm plantation.Furthermore,through a multivariate approach,we explored the main drivers of reproductive output in N.atrata.Results showed that prey abundance and food intake were crucial variables contributing to reproductive output.Multiple linear regression models showed that neonates had high sensitivity(R2=55.29%)to extreme changes in climate,which was strongly related to slug and snail abundance variability and microhabitat quality.Reproductive cycles were markedly different between the sexes,being continuous in males and cyclical in females.Despite this variation,reproductive cycles at the population level were seasonal semi-synchronous.Constant recruitment of neonates all year,multiple clutches,high mating frequency,and continuous sperm production characterized the reproductive phenology of N.atrata.In addition,a significant number of previtellogenic females presented oviductal sperm as well as uterine scars,suggesting a high precocity in the species.The main drivers of reproductive output also differed between the sexes.In females,clutch size and secondary follicle variability were highly related to stomach bolus volume,fat body area,and body mass.In males,height of piles of palm leaves and body mass,rather than intrinsic reproductive traits,were the main drivers of sperm production.Nevertheless,in both cases,the relationship between body mass,prey abundance,and food intake suggests that N.atrata follows the income breeding strategy to compensate for reproductive costs and to maximize fitness. 展开更多
关键词 Continuous male reproduction CLUTCH mass Income breeding Iteroparity SPERMATOGENESIS OOGENESIS REPRODUCTIVE EFFORT El Ni?o-Southern Oscillation(ENSO)
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