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不锈钢锰磷化膜重测量的改进研究 预览
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作者 穆俊宏 刘晓燕 朱建国 《广州化工》 CAS 2019年第13期135-137,150共4页
重量法测量锰磷化膜重时,常规测量过程中使用铬酐易造成对操作者健康的损害和对环境的污染,本文用乙二胺四乙酸二钠和氢氧化钠的组合试剂替代铬酐。研究表明,当乙二胺四乙酸二钠浓度为150~170g/L,氢氧化钠浓度为150~170g/L,与常规方法... 重量法测量锰磷化膜重时,常规测量过程中使用铬酐易造成对操作者健康的损害和对环境的污染,本文用乙二胺四乙酸二钠和氢氧化钠的组合试剂替代铬酐。研究表明,当乙二胺四乙酸二钠浓度为150~170g/L,氢氧化钠浓度为150~170g/L,与常规方法的相对测量误差在5%以内,符合膜重测量数据的准确度要求,且无需加热、操作简便、环境友好。 展开更多
关键词 锰磷化 膜重测量 替代 铬酐 乙二胺四乙酸二钠和氢氧化钠
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Decreased numbers of circulating endothelial progenitor cells are associated with hyperglycemia in patients with traumatic brain injury 预览
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作者 Hui-Jie Wei Li Liu +7 位作者 Fang-Lian Chen Dong Wang Liang Wang Zeng-Guang Wang Rong-Cai Jiang Jing-Fei Dong Jie-Li Chen Jian-Ning Zhang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期984-990,共7页
Hyperglycemia reduces the number of circulating endothelial progenitor cells,accelerates their senescence and impairs their function.However,the relationship between blood glucose levels and endothelial progenitor cel... Hyperglycemia reduces the number of circulating endothelial progenitor cells,accelerates their senescence and impairs their function.However,the relationship between blood glucose levels and endothelial progenitor cells in peripheral blood of patients with traumatic brain injury is unclear.In this study,101 traumatic brain injury patients admitted to the Department of Neurosurgery,Tianjin Medical University General Hospital or the Department of Neurosurgery,Tianjin Huanhu Hospital,China,were enrolled from April 2005 to March 2007.The number of circulating endothelial progenitor cells and blood glucose levels were measured at 1,4,7,14 and 21 days after traumatic brain injury by flow cytometry and automatic biochemical analysis,respectively.The number of circulating endothelial progenitor cells and blood sugar levels in 37 healthy control subjects were also examined.Compared with controls,the number of circulating endothelial progenitor cells in traumatic brain injury patients was decreased at 1 day after injury,and then increased at 4 days after injury,and reached a peak at 7 days after injury.Compared with controls,blood glucose levels in traumatic brain injury patients peaked at 1 day and then decreased until 7 days and then remained stable.At 1,4,and 7 days after injury,the number of circulating endothelial progenitor cells was negatively correlated with blood sugar levels(r=?0.147,P<0.05).Our results verify that hyperglycemia in patients with traumatic brain injury is associated with decreased numbers of circulating endothelial progenitor cells.This study was approved by the Ethical Committee of Tianjin Medical University General Hospital,China(approval No.200501)in January 2015. 展开更多
关键词 nerve REGENERATION endothelial progenitor cells VASCULAR repair VASCULAR remodeling angiogenesis NEOVASCULARIZATION blood glucose HYPERGLYCEMIA traumatic BRAIN injury MOBILIZATION suppression senescence alternative therapy BRAIN damage neural REGENERATION
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Chenopodium ambrosioides induces an endothelium-dependent relaxation of rat isolated aorta
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作者 Asmae Assaidi Ikram Dib +9 位作者 Monique Tits Luc Angenot Said Bellahcen Nourelhouda Bouanani Abdelkhaleq Legssyer Mohammed Aziz Hassane Mekhfi Mohammed Bnouham Michel Frederich Abderrahim Ziyyat 《结合医学学报:英文版》 CAS CSCD 2019年第2期115-124,共10页
Objective: This study aims to evaluate the vasodilatory effect of Chenopodium ambrosioides on the isolated rat aorta, and to explore its mechanism of action. Methods: The vasorelaxant effect and the mode of action of ... Objective: This study aims to evaluate the vasodilatory effect of Chenopodium ambrosioides on the isolated rat aorta, and to explore its mechanism of action. Methods: The vasorelaxant effect and the mode of action of various extracts from the leaves of C. ambrosioides were evaluated on thoracic aortic rings isolated from Wistar rats. In addition, ethyl acetate and methanol fractions were analyzed, using thin-layer chromatography and high-performance liquid chromatography techniques, for their polyphenolic con tent. Results: The various active extracts of C. ambrosioides at four concentrations (10 3, 10 2, 10 1* and 1 mg/mL) relaxed the con traction elicited by phe ny lephri ne, in a concentration-depende nt manner. This effect seems to be endothelium-dependent, since the vasodilatory effect was entirely absent in denuded aortic rings. The vasorelaxant effect of the methanol fraction (MF) of C. ambrosioides at 1 mg/mL was also inhibited by atropine and tetraethylammonium. This effect remained unchanged by Nw-nitro-L-arginine methyl ester hydrochloride and glibenclamide. The preliminary phytochemical analysis showed that the leaves of C. ambrosioides are rich in phenolic and flavonoid derivatives. Conclusion: These results suggest that the MF of C. ambrosioides produces an endothelium-dependent relaxation of the isolated rat aorta, which is thought to be mediated mainly through stimulation of the muscarinic receptors, and probably involving the opening of Ca^2+-activated potassium channels. 展开更多
关键词 CHENOPODIUM ambrosioides CHENOPODIACEAE VASORELAXATION Ca^2+-activated potassium channels THIN-LAYER CHROMATOGRAPHY High-performance liquid CHROMATOGRAPHY Plant Complementary and alternative medicine
Blood-Letting Therapy for Hypertension: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
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作者 XIONG Xing-jiang WANG Peng-qian LI Sheng-jie 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第2期139-146,共8页
Objective:To evaluate the efficacy and safety of blood-letting therapy(BLT)in treatment of hypertension.Methods:A comprehensive electronic and manual bibliographic searches were performed in Cochrane Central Register ... Objective:To evaluate the efficacy and safety of blood-letting therapy(BLT)in treatment of hypertension.Methods:A comprehensive electronic and manual bibliographic searches were performed in Cochrane Central Register of Controlled Trials,Excerpt Medica Database(EMBASE),PubMed,China National Knowledge Infrastructure,Chinese Scientific Journal Database,Chinese Biomedical Literature Database,and Wanfang Database to identify randomized controlled trials(RCTs)in which hypertensive patients were treated with BLT or BLT plus antihypertensive drugs(BPAD)against placebo,no treatment or antihypertensive drugs.The Cochrane Risk Assessment Tool was used to assess the methodological quality of trials.The Review Manager 5.3 software was used for meta-analysis.Results:A total of 7 RCTs with 637 hypertensive patients from 1989 to 2017 were identified.Compared with antihypertensive drugs,blood pressure was significantly reduced by BLT(RR=1.21,95%CI:1.01 to 1.44,P=0.03;heterogeneity:P=0.06,I^2=60%)and BPAD(RR=1.25,95%CI,1.02 to 1.53,P=0.03;heterogeneity:P=0.01,I^2=71%).Moreover,a significant improvement in Chinese medicine syndrome by BLT(RR=1.32;95%CI:1.14 to 1.53,P=0.0002;heterogeneity:P=0.53,I^2=0%)and BPAD(RR=1.47;95%CI:1.06 to 2.04,P=0.02;heterogeneity:P=0.13,I^2=56%)was identified.The reported adverse effects were well tolerated.Conclusions:Although some positive findings were identified,no definite conclusions regarding the efficacy and safety of BLT as complementary and alternative approach for treatment of hypertension could be drew due to the generally poor methodological design,significant heterogeneity,and insufficient clinical data.Further rigorously designed trials are warranted to confirm the results. 展开更多
关键词 blood-letting THERAPY PHLEBOTOMY BLOOD pressure Chinese MEDICINE COMPLEMENTARY and alternative MEDICINE systematic review META-ANALYSIS randomized controlled trials
黄河中游(渭南-郑州段)全/多氟烷基化合物的分布及通量
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作者 李琦路 程相会 +5 位作者 赵祯 郭萌然 袁梦 华夏 方祥光 孙红文 《环境科学》 EI CAS CSCD 北大核心 2019年第1期228-238,共11页
本研究收集黄河中游(渭南-郑州段)表层水样品,利用高效液相色谱质谱串联的方法分析了水相和颗粒相中的28种全氟和多氟烷基化合物(PFASs).结果表明,水相和颗粒相中∑28PFASs的含量分别为18.4~56.9 ng·L^-1和26.8~164 ng·g^-1(... 本研究收集黄河中游(渭南-郑州段)表层水样品,利用高效液相色谱质谱串联的方法分析了水相和颗粒相中的28种全氟和多氟烷基化合物(PFASs).结果表明,水相和颗粒相中∑28PFASs的含量分别为18.4~56.9 ng·L^-1和26.8~164 ng·g^-1(以干重计).水相和颗粒相中以全氟己酸(PFHxA)为主要污染物,分别占总含量的27%和16%,且3H-全氟-3-(3-甲氧基丙氧基)丙酸(ADONA)、氯代多氟醚基磺酸(6:2和8:2 Cl-PFESA)在颗粒相均有检出,表明PFASs替代品的生产和使用逐渐增多.PFASs在水相-颗粒相中的lgKd变化范围为2.95±0.553(PFPeA)~3.85±0.237(8:2 FTUCA),颗粒物吸附氟调聚羧酸(FTCAs)和不饱和氟调聚羧酸(FTUCAs)的能力随碳链长度的增长而增加,全氟烷基磺酸(PFSAs)较全氟烷基羧酸(PFCAs)更容易被颗粒物吸附.黄河郑州-渭南段PFASs的通量呈现先降低后增加的趋势,表明该河段接纳了来自上游及支流的污染输入.此外,结果表明水相中的PFASs通量大于颗粒相. 展开更多
关键词 全/多氟烷基化合物(PFASs) 黄河 替代品 分配系数 通量
Deep Learning Deepens the Analysis of Alternative Splicing
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作者 Xudong Zou Xin Gao Wei Chen 《基因组蛋白质组与生物信息学报:英文版》 CAS CSCD 2019年第2期219-221,共3页
The ever-increasing high-volume and high-dimensional geno-mics data on the one hand challenge traditional data analysis approaches,and on the other hand provide ample opportuni-ties for developing novel analytic strat... The ever-increasing high-volume and high-dimensional geno-mics data on the one hand challenge traditional data analysis approaches,and on the other hand provide ample opportuni-ties for developing novel analytic strategies. 展开更多
关键词 DEEP LEARNING Deepens the ANALYSIS ALTERNATIVE SPLICING
Alternative Assessment in an EFL Project-based Learning Context:Per.ceptions and Correlations 预览
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作者 韦启卫 皮拉萨·西里约丁 安德鲁·彼得·莱恩 《海外英语》 2019年第12期272-277,共6页
This study aims to explore Chinese university EFL learners'perceptions toward alternative assessment in a context of a project-based learning digital storytelling presentation in Speaking Course.It also seeks to c... This study aims to explore Chinese university EFL learners'perceptions toward alternative assessment in a context of a project-based learning digital storytelling presentation in Speaking Course.It also seeks to compare the relationship between alternative assessment and teacher assessment.The findings showed that a strong correlation between alternative assessment and teacher assessment occurred.Alternative assessment activities are viewed by students as"authentic"assessments,as they mimic how the student will be using their knowledge in the future.Alternative assessment as a form of formative assessment can be a powerful day-to-day tool for teachers and students.Alternative assessment is an enabler of process of learning.The study suggests that alternative assessment can encourage learners to become more fully responsible for their learning and can result in more and better learning.Alternative assessment can thus be used as a golden key to the"deaf and dumb"phenomenon for Chinese university EFL learners. 展开更多
关键词 ALTERNATIVE assessment EFL PROJECT-BASED learning digital storytelling speaking course
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XEN gel stent: a total delayed-onset postoperative hyphema
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作者 Amina Rezkallah Thibaud Mathis +1 位作者 Philippe Denis Laurent Kodjikian 《国际眼科杂志:英文版》 SCIE CAS 2019年第7期1224-1226,共3页
Dear Editor,Minimally invasive glaucoma surgery (MIGS) is a lessinvasive surgical alternative to filtering surgery for reducing intraocular pressure (IOP). This technique has been shown to offer long-term outcomes equ... Dear Editor,Minimally invasive glaucoma surgery (MIGS) is a lessinvasive surgical alternative to filtering surgery for reducing intraocular pressure (IOP). This technique has been shown to offer long-term outcomes equivalent to filtering surgery[1]. The XEN gel implant (Allergan Inc., CA, USA) is one of MIGS procedures. This implant consists of a 6-mm tube of collagen-derived gelatin cross-linked with glutaraldehyde, preloaded in an injector. 展开更多
关键词 MINIMALLY invasive GLAUCOMA surgery(MIGS) lessinvasive surgical alternative reducing INTRAOCULAR pressure(IOP)
Cinobufacini injection for moderate and advanced primary liver cancer: A systematic review and meta-analysis
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作者 Zhiyong Dong Xiantu Qiu +2 位作者 Stacy A. Kujawa S.M. Shariful Islam Jie Zhang 《中国药学:英文版》 CAS CSCD 2019年第4期264-275,共12页
Cinobufocini, a compound extracted from bufo toad, is a traditional Chinese medicine (TCM) that has been shown to have efficacy in cancer treatment. The cinobufacini injection is widely used in patients with moderate ... Cinobufocini, a compound extracted from bufo toad, is a traditional Chinese medicine (TCM) that has been shown to have efficacy in cancer treatment. The cinobufacini injection is widely used in patients with moderate and advanced liver cancer in China. In the present study, we aimed to determine the effects of cinobufacini injection for this specific category of patients. A search for randomized control trials (RCTs) using cinobufacini was conducted in the PubMed, EMBASE, CENTRAL, and the other four major Chinese databases. The systematic review was performed according to the recommendations of the Cochrane collaboration, and the RevMan 5.3. software was used for statistical analysis. A random-effects model was used to perform the data. Risk ratios (RR) with corresponding 95% confide nee interval were calculated according to Cochrane handbook. A total of 11 RCTs consisting of 728 patients were included. All of the trials demonstrated significantly improved total response rates, total response rates of Kamofsky Performanee Score (KPS), 1- to 2-year survival rates, and quality of life in the intervention groups injected with cinobufacini. There was no statistically significant difference between the groups (cinobufacini versus no cinobufacini;cinobufacini plus transcatheter arterial chemoembolization (TACE) versus TACE only) in terms of the 6-month survival rate, clinical benefit rate and clinical benefit rate of KPS. This systematic review demonstrated the beneficial effects of cinobufacini injection in terms of total response rate and the 1- to 2-year survival rate in patients with moderate and advanced liver cancer. The efficacy for the cinobufacini injection group might be better than that of the control group for treatment of moderate and advanced liver cancer. Given that the majority of the trials were of low quality, more high-quality prospective RCTs with strict design in accordance with CONSORT for TCM are needed. 展开更多
关键词 CINOBUFACINI injection Liver cancer META-ANALYSIS Chinese HERB medicine ALTERNATIVE THERAPIES
A test method for evaluating the thermal environment of underground coal mine refuge alternatives 预览
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作者 D.S.Yantek L.Yan +2 位作者 N.W.Damiano M.A.Reyes J.R.Srednicki 《矿业科学技术学报:英文版》 CSCD 2019年第3期343-355,共13页
Since 2009,the Mine Safety and Health Administration(MSHA)has required mines to install refuge alternatives(RAs)in underground coal mines.One of the biggest concerns with occupied RAs is the possible severity of the r... Since 2009,the Mine Safety and Health Administration(MSHA)has required mines to install refuge alternatives(RAs)in underground coal mines.One of the biggest concerns with occupied RAs is the possible severity of the resulting thermal environment.In 30 CFR 7.504,the maximum allowable apparent temperature(AT)for an occupied RA is specified as 35℃(95°F).Manufacturers must conduct heat/humidity tests to demonstrate that their RAs meet the 35℃(95°F)AT limit.For these tests,heat input devices are used to input the metabolic heat of actual miners.A wide variety of test methods,sensors,and heat input devices could be used when conducting such tests.Since 2012,the National Institute for Occupational Safety and Health(NIOSH)has conducted over thirty 96-hour heat/humidity tests on four different RAs.This paper discusses the test equipment and procedures used during these investigations.This information is useful for RA manufacturers conducting RA heat/humidity tests,for other researchers investigating RA heat/humidity buildup,and for those who need to assess the thermal environment of any confined space where people may be trapped or are seeking refuge. 展开更多
关键词 REFUGE CHAMBER REFUGE ALTERNATIVE CONFINED space THERMAL environment Test method Temperature
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Situating Mandarin Wh-indefinites in the Typology of Modal Indefinites
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作者 刘明明 崔延燕 《外国语》 CSSCI 北大核心 2019年第3期26-37,共12页
This paper discusses the non-interrogative existential construal of Mandarin wh-items.Different from previous works that mainly focus on distribution of these items (Li, 1992;Lin, 1998;Xie, 2007), the paper emphasizes... This paper discusses the non-interrogative existential construal of Mandarin wh-items.Different from previous works that mainly focus on distribution of these items (Li, 1992;Lin, 1998;Xie, 2007), the paper emphasizes their interpretation.A wh-indefinite is shown to trigger distinct modal inferences in different contexts.By making comparisons with Spanish algún (Alonso-Ovalle & Menéndez-Benito, 2010), the paper situates Mandarin wh-indefinites in the typology of modal indefinites across languages. 展开更多
关键词 wh-items MODAL indefinites alternative semantics
花卉无土栽培泥炭基质的替代研究 预览
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作者 周惠民 何丽斯 +3 位作者 李畅 刘晓青 肖政 苏家乐 《江苏林业科技》 2019年第3期45-48,共4页
根据国内外花卉无土栽培中泥炭基质的替代需求,概述了泥炭基质替代研究的进展。并结合我国国情,详细介绍了近20a国内利用几种典型的有机废弃物(菇渣、园林绿化废弃物、城市污泥、秸秆、生物质炭)开发花卉固体基质的研究成果与发展前景,... 根据国内外花卉无土栽培中泥炭基质的替代需求,概述了泥炭基质替代研究的进展。并结合我国国情,详细介绍了近20a国内利用几种典型的有机废弃物(菇渣、园林绿化废弃物、城市污泥、秸秆、生物质炭)开发花卉固体基质的研究成果与发展前景,并进一步指出影响国内泥炭替代基质发展的主要原因,提出相应的发展对策。 展开更多
关键词 花卉 无土栽培 泥炭基质 替代 秸秆
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Dek42 encodes an RNA-binding protein that affects alternative pre-mRNA splicing and maize kernel development^FA
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作者 Yi Zuo Fan Feng +1 位作者 Weiwei Qi Rentao Song 《植物学报:英文版》 SCIE CAS CSCD 2019年第6期728-748,共21页
RNA-binding proteins (RBPs) play an important role in post-transcriptional gene regulation. However, the functions of RBPs in plants remain poorly understood. Maize kernel mutant dek42 has small defective kernels and ... RNA-binding proteins (RBPs) play an important role in post-transcriptional gene regulation. However, the functions of RBPs in plants remain poorly understood. Maize kernel mutant dek42 has small defective kernels and lethal seedlings. Dek42 was cloned by Mutator tag isolation and further confirmed by an independent mutant allele and clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 materials. Dek42 encodes an RRM_RBM48 type RNA-binding protein that localizes to the nucleus. Dek42 is constitutively expressed in various maize tissues. The dek42 mutation caused a significant reduction in the accumulation of DEK42 protein in mutant kernels. RNA-seq analysis showed that the dek42 mutation significantly disturbed the expression of thousands of genes during maize kernel development. Sequence analysis also showed that the dek42 mutation significantly changed alternative splicing in expressed genes, which were especially enriched for the U12-type intron-retained type. Yeast two-hybrid screening identified SF3a1 as a DEK42-interacting protein. DEK42 also interacts with the spliceosome component U1-70K. These results suggested that DEK42 participates in the regulation of pre-messenger RNA splicing through its interaction with other spliceosome components. This study showed the function of a newly identified RBP and provided insights into alternative splicing regulation during maize kernel development. 展开更多
关键词 Dek42 ENCODES an RNA-BINDING protein that AFFECTS ALTERNATIVE pre-mRNA splicing and MAIZE kernel development^FA Dek42 ENCODES
红外电子额温计替代水银体温计的临床应用探索 预览
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作者 杨维秀 金花成 《中国继续医学教育》 2019年第14期175-177,共3页
目的调查传统水银体温计与红外电子额温计测量患者体温的差异,探索在患者日常体温监测时能否用红外电子额温计进行取代。方法以儿科为实验科室,以水银温度计的测量值划分体温正常组与发热组,每次同时使用两种方法分别测量患儿体温并进... 目的调查传统水银体温计与红外电子额温计测量患者体温的差异,探索在患者日常体温监测时能否用红外电子额温计进行取代。方法以儿科为实验科室,以水银温度计的测量值划分体温正常组与发热组,每次同时使用两种方法分别测量患儿体温并进行配对分析,一致性检验采用Kappa分析。结果水银体温计与红外电子额温计对体温正常者腋温、额温的测量结果比较,差异无统计学意义(t=1.491,P=0.136),但对发热患者腋温、额温的测量结果比较,差异具有统计学意义(t=9.177,P<0.001)。两种方法经Kappa一致性检验,Kappa值为0.815,灵敏度为71.72%,特异度为99.85%,说明水银体温计与红外电子额温计两种方法测量结果具有较好的一致性。结论对于体温正常者,可用更方便的红外电子额温计进行测量,提高护理人员工作效率。但对于发热者,还是要继续采用水银体温计的测量结果作为金标准。 展开更多
关键词 电子额温计 水银体温计 测量 体温值 发热 替代
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Medicine has always been "Modern" and “Scientific” from ancient times to the present day
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作者 Mikhail Teppone 《结合医学学报:英文版》 CAS CSCD 2019年第4期229-237,共9页
Throughout human history, doctors and healers have gathered and refined the knowledge inherited from the previous generations. Different methods of effective therapy have been designed during various historical period... Throughout human history, doctors and healers have gathered and refined the knowledge inherited from the previous generations. Different methods of effective therapy have been designed during various historical periods;when each was developed, it was considered "modern scientific medicine" for their time. Mankind has gone through natural and social disasters and survived;hence, history has proved there was no time when medical knowledge was eironeous or ineffective. Classic medicine has grown to be divided into narrow, specialized branches, causing it to lose its holistic approach and general view on health, sickness and therapeutic methods. Many of traditional medicine's effective methods have been forgotten and removed from the mainstream medicine. It would be good for modern medical education to incorporate the general knowledge of historically effective therapeutic modalities and study practical cases. Medical students should be taught how to choose a "good method" or "good medicine" independent of when that method or remedy was discovered. However, he has to keep in mind the primary goal of medicine:"I will use treatment to help the sick according to my ability and judgment..."(from Hippocratic Oath). 展开更多
关键词 INTEGRATIVE MEDICINE Alternative MEDICINE Traditional Chinese MEDICINE HOMEOPATHY Cellular therapy
RNA binding protein 24 deletion disrupts global alternative splicing and causes dilated cardiomyopathy
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作者 Jing Liu Xu Kong +2 位作者 Mengkai Zhang Xiao Yang Xiuqin Xu 《蛋白质与细胞:英文版》 SCIE CAS CSCD 2019年第6期405-416,共12页
RNA splicing contributes to a broad spectrum of posttranscriptional gene regulation during normal development, as well as pathological manifestation of heart diseases. However, the functional role and regulation of sp... RNA splicing contributes to a broad spectrum of posttranscriptional gene regulation during normal development, as well as pathological manifestation of heart diseases. However, the functional role and regulation of splicing in heart failure remain poorly understood. RNA binding protein (RBP), a major component of the splicing machinery, is a critical factor in this process. RNA binding motif protein 24 (RBM24) is a tissue-specific RBP which is highly expressed in human and mouse heart. Previous studies demonstrated the functional role of RBM24 in the embryonic heart development. However, the role of RBM24 in postnatal heart development and heart disease has not been investigated. In this paper, using conditional RBM24 knockout mice, we demonstrated that ablation of RBM24 in postnatal heart led to rapidly progressive dilated cardiomyopathy (DCM), heart failure, and postnatal lethality. Global splicing profiling revealed that RBM24 regulated a network of genes related to cardiac function and diseases. Knockout of RBM24 resulted in misregulation of these splicing transitions which contributed to the subsequent development of cardiomyopathy. Notably, our analysis identified RBM24 as a splice factor that determined the splicing switch of a subset of genes in the sacomeric Z-disc complex, including Titin, the major disease gene of DCM and heart failure. Together, this study identifies regulation of RNA splicing by RBM24 as a potent player in remodeling of heart during postnatal development, and provides novel mechanistic insights to the pathogenesis of DCM. 展开更多
关键词 RNA binding protein RBM24 DILATED CARDIOMYOPATHY alternative SPLICING heart failure
An Online Alternative
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作者 Ji Jing 《北京周报:英文版》 2019年第19期44-45,共2页
After runaway growth, Web films begin to focus on quality When Wang Guandi’s first film Championin Vilage was released last year, he was thankful for the new genre of moviest hat has made it possible for newcomers li... After runaway growth, Web films begin to focus on quality When Wang Guandi’s first film Championin Vilage was released last year, he was thankful for the new genre of moviest hat has made it possible for newcomers like him to realize their dream of making films. 展开更多
关键词 In AN ONLINE ALTERNATIVE WEB FILMS
Preparation and Spectroscopic of Vanadyl(Ⅱ) Vitamin D3 Amino Acid Mixed Complexes as Insulin Mimetic Drug 预览
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作者 Enas Aljuhani Amnah M.A.Alsuhaibani +3 位作者 A.M.El-Di damony N.Hassan Sameh Abo Taleb Moamen S.Refat 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2019年第7期2316-2324,共9页
A new six intraperitoneal injection insulin-mimetic vanadyl(Ⅱ) compounds [(VD3^-1)(VO^+2)(AAn^-1)](where(n=1~6);AA1=isoleucine, AA2=threonine, AA3=proline, AA4=phenylalanine, AA5=lysine and AA6=glutamine) were synthe... A new six intraperitoneal injection insulin-mimetic vanadyl(Ⅱ) compounds [(VD3^-1)(VO^+2)(AAn^-1)](where(n=1~6);AA1=isoleucine, AA2=threonine, AA3=proline, AA4=phenylalanine, AA5=lysine and AA6=glutamine) were synthesized by the chemical reactions between vitamin D3(VD3), VOSO4 and amino acids(AAn) with equal molar ratio 1∶1∶1 in neutralized media. The structures of these complexes were elucidated by spectroscopic methods like, infrared and solid reflectance spectroscopes. Magnetic moments and electronic spectra reveal square pyramid geometrical structure of the complexes. The infrared spectra assignments of these complexes revealed that the chelation towards vanadyl(Ⅳ) ions existed via deprotonation of the hydroxyl group of VD3 drug ligand and so amino acids act as bidentate ligand via N-amino and O-carboxylate groups. The anti-diabetic efficiency of these complexes were evaluated against streptozotocin induced diabetic male albino rats. 展开更多
关键词 INSULIN alternative Diabetes DRUG VO^2+ ion Vitamin D3 Amino acid SPECTROSCOPIC
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逆全球化与豆粕替代方案研究
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作者 崔宁波 徐杭 刘姝君 《大豆科学》 CAS CSCD 北大核心 2019年第5期786-792,共7页
中国大豆市场需求构成主要来自大豆、豆油和豆粕,且进口大豆80%用于生产豆粕,在贸易摩擦紧张时期大豆的供给缺口直接导致豆粕的价格涨幅迅速,而近期的贸易摩擦缓和与大范围非洲猪瘟频发又导致豆粕走势较弱,豆粕价格的大幅波动一定程度... 中国大豆市场需求构成主要来自大豆、豆油和豆粕,且进口大豆80%用于生产豆粕,在贸易摩擦紧张时期大豆的供给缺口直接导致豆粕的价格涨幅迅速,而近期的贸易摩擦缓和与大范围非洲猪瘟频发又导致豆粕走势较弱,豆粕价格的大幅波动一定程度上限制了我国饲料及养殖行业的发展。因此本文对如何通过改变豆粕的市场占有量,降低豆粕价格波动对于相关行业的影响,寻找可以带来更高经济效益的低含量豆粕饲料替代产品进行研究。以生猪为例,结合其生长发育所需最低营养标准建立了28个约束条件,将最为关键的成本费用问题确定为目标函数,运用Lingo软件求最优解。通过分析建模,得到现有条件下最合理的替代选择方案,对模型进行了灵敏度分析,验证其有效性,并对该模型的可推广性进行分析。 展开更多
关键词 逆全球化 豆粕 替代方案 优化分析
Identification of novel mouse and rat CB1R isoforms and in silico modeling of human CB1R for peripheral cannabinoid therapeutics
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作者 Qing-Rong Liu Nicholas S.Huang +8 位作者 Hong Qu Jennifer F.O′Connell Isabel Gonzalez-Mariscal Sara Santa-Cruz-Calvo Maire E.Doyle Zheng-Xiong.Xi Yun Wang Emmanuel.S.Onaivi Josephine M.Egan 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第3期387-397,共11页
Targeting peripheral CB1R is desirable for the treatment of metabolic syndromes without adverse neuropsychiatric effects.We previously reported a human hCB1b isoform that is selectively enriched in pancreatic beta-cel... Targeting peripheral CB1R is desirable for the treatment of metabolic syndromes without adverse neuropsychiatric effects.We previously reported a human hCB1b isoform that is selectively enriched in pancreatic beta-cells and hepatocytes,providing a potential peripheral therapeutic hCB1R target.It is unknown whether there are peripherally enriched mouse and rat CB1R(mCB1 and rCB1,respectively)isoforms.In this study,we found no evidence of peripherally enriched rodent CB1 isoforms;however,some mCB1R isoforms are absent in peripheral tissues.We show that the mouse Cnr1 gene contains six exons that are transcribed from a single promoter.We found that mCB1A is a spliced variant of extended exon 1 and protein-coding exon 6;mCB1B is a novel spliced variant containing unspliced exon 1,intron 1,and exon 2,which is then spliced to exon 6;and mCB1C is a spliced variant including all 6 exons.Using RNAscope in situ hybridization,we show that the isoforms mCB1A and mCB1B are expressed at a cellular level and colocalized in GABAergic neurons in the hippocampus and cortex.RT-qPCR reveals that mCB1A and mCB1B are enriched in the brain,while mCB1B is not expressed in the pancreas or the liver.Rat rCB1R isoforms are differentially expressed in primary cultured neurons,astrocytes,and microglia.We also investigated modulation of Cnr1 expression by insulin in vivo and carried out in silico modeling of CB1R with JD5037,a peripherally restricted CB1R inverse agonist,using the published crystal structure of hCB1R.The results provide models for future CB1R peripheral targeting. 展开更多
关键词 CANNABINOIDS cell-and tissue-based therapy alternative SPLICING gene expression
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