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文章速递Role of PV-Powered Vehicles in Low-Carbon Society and Some Approaches of High-Efficiency Solar Cell Modules for Cars 认领
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作者 Masafumi Yamaguchi Taizo Masuda +11 位作者 Kenji Araki Daisuke Sato Kan-Hua Lee Nobuaki Kojima Tatsuya Takamoto Kenichi Okumura Akinori Satou Kazumi Yamada Takashi Nakado Yusuke Zushi Mitsuhiro Yamazaki Hiroyuki Yamada 《能源与动力工程(英文)》 2020年第6期375-395,共21页
Development of highly-efficient photovoltaic (PV) modules and expanding its application fields are significant for the further development of PV technologies and realization of innovative green energy infrastructure b... Development of highly-efficient photovoltaic (PV) modules and expanding its application fields are significant for the further development of PV technologies and realization of innovative green energy infrastructure based on PV. Especially, development of solar-powered vehicles as a new application is highly desired and very important for this end. This paper presents the impact of PV cell/module conversion efficiency on reduction in CO</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> emission and increase in driving range of the electric based vehicles. Our studies show that the utilization of a highly-efficient (higher than 30%) PV module enables the solar-powered vehicle to drive 30 km/day without charging in the case of light weig</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">h</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">t cars with elec</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">t</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ric mileage of 17</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">km/kWh under solar irrad</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">i</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">a</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">t</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ion of 3.7</span></span></span><span><span><span style="font-family:""> </span></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">kWh/m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;">/day, which means that the majority of the family cars in Japan can run only by the sunlight without supplying fossil fuels. Thus, it is essential to develop high-efficiency as well as low-cost solar cells and modules for automotive applications. The analytical results developed by the authors for conversion efficiency potential of various solar cells for choosing candidates of the PV modules for automotive applications are shown. Then we overview the conversion efficiency potential and recent progress of various Si tandem solar cells, such as III-V/Si, II-VI/Si, chalcopyrite/Si, and perovskite/Si tandem solar cells. The III-V/Si tandem solar cells are expected to have a high potential for various applications because of its high conversion efficiency of larger than 36% for dual-junction and 42% for triple-junction solar cells under 1-sun AM1.5 G illumination, lightweight and low-cost potentials. The analysis show</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> that III-V based multi-junction and Si based tandem solar cells are considered to be promising candidates for the automotive application. Finally, we report recent results for our 28.2% efficiency and Sharp’s 33% mechanically stacked InGaP/GaAs/Si triple-junction solar cell. In addition, new approaches which </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">are</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> suitable for automotive applications by using III-V triple-junction, and static low concentrator PV modules are also presented. 展开更多
关键词 Solar Cell Powered Vehicle Applications High-Efficiency Solar Cells Multi-Junction Solar Cells Tandem Solar Cells Modules
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文章速递Proteomic profiling of various human dental stem cells-a systematic review 认领
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作者 Jagadish Hosmani Khalil Assiri +7 位作者 Hussain Mohammed Almubarak Master Luqman Mannakandath Ahmed Al-Hakami Shankargouda Patil Deepa Babji Sachin Sarode Anantharam Devaraj Harish C Chandramoorthy 《世界干细胞杂志:英文版(电子版)》 SCIE CAS 2020年第10期1214-1236,共23页
BACKGROUND The proteomic signature or profile best describes the functional component of a cell during its routine metabolic and survival activities.Additional complexity in differentiation and maturation is observed ... BACKGROUND The proteomic signature or profile best describes the functional component of a cell during its routine metabolic and survival activities.Additional complexity in differentiation and maturation is observed in stem/progenitor cells.The role of functional proteins at the cellular level has long been attributed to anatomical niches,and stem cells do not deflect from this attribution.Human dental stem cells(hDSCs),on the whole,are a combination of mesenchymal and epithelial coordinates observed throughout craniofacial bones to pulp.AIM To specify the proteomic profile and compare each type of hDSC with other mesenchymal stem cells(MSCs)of various niches.Furthermore,we analyzed the characteristics of the microenvironment and preconditioning changes associated with the proteomic profile of hDSCs and their influence on committed lineage differentiation.METHODS Literature searches were performed in PubMed,EMBASE,Scopus,and Web of Science databases,from January 1990 to December 2018.An extra inquiry of the grey literature was completed on Google Scholar,ProQuest,and OpenGrey.Relevant MeSH terms(PubMed)and keywords related to dental stem cells were used independently and in combination.RESULTS The initial search resulted in 134 articles.Of the 134 full-texts assessed,96 articles were excluded and 38 articles that met the eligibility criteria were reviewed.The overall assessment of hDSCs and other MSCs suggests that differences in the proteomic profile can be due to stem cellular complexity acquired from varied tissue sources during embryonic development.However,our comparison of the proteomic profile suffered inconsistencies due to the heterogeneity of various hDSCs.We believe that the existence of a heterogeneous population of stem cells at a given niche determines the modalities of regeneration or tissue repair.Added prominences to the differences present between various hDSCs have been reasoned out.CONCLUSION Systematic review on proteomic studies of various hDSCs are promising as an eye-opener for revisiting the proteomic profile and in-depth analysis to elucidate more refined mechanisms of hDSC functionalities. 展开更多
关键词 Apical papilla stem cells Dental follicle stem cells Dental pulp stem cells Periodontal ligament stem cells Proteomics
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文章速递Identification of a Native Novel Oncolytic Immunoglobulin on Exfoliated Colon Epithelial Cells: A Bispecific Heterodimeric Chimera of IgA/IgG* 认领
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作者 George P. Albaugh Sudhir K. Dutta +14 位作者 Vasantha Iyengar Samina Shami Althaf Lohani Eduardo Sainz George Kessie Prasanna Nair Sara Lagerholm Alka Kamra J.-H. Joshua Chen Shilpa Kalavapudi Rakesh Vinayek Robert Shores Laila E. Phillips Ram Nair Padmanabhan P. Nair 《预防医学期刊(英文)》 2020年第6期126-150,共25页
Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (... Understanding the nature of cell surface markers on exfoliated colonic cells is a crucial step in establishing criteria for a normally functioning mucosa. We have found that colonic cells isolated from stool samples (SCSR-010 Fecal Cell Isolation Kit, NonInvasive Technologies, Elkridge, MD), preserved at room temperature for up to one week, with viability of >85% and low levels of apoptosis (8% - 10%) exhibit two distinct cell size subpopulations, in the 2.5 μM - 5.0 μM and 5.0 μM - 8.0 μM range. In addition to IgA, about 60% of the cells expressed a novel heterodimeric IgA/IgG immunoglobulin that conferred a broad-spectrum cell mediated cytotoxicity against tumor cells. In a cohort of 58 subjects the exclusive absence of this immunoglobulin in two African-Americans was suggestive of a germline deletion. Serial cultures in stem cell medium retained the expression of this heterodimer. Since a majority of the cystic cells expressed the stem cell markers Lgr5 and Musashi-1 we termed these cells as gastrointestinal progenitor stem cells (GIP-C**). CXCR-4, the cytokine co-receptor for HIV was markedly expressed. These cells also expressed CD20, IgA, IgG, CD45, and COX-2. We assume that they originated from mature columnar epithelium by dedifferentiation. Our observations indicate that we have a robust noninvasive method to study mucosal pathophysiology and a direct method to create a database for applications in regenerative medicine. 展开更多
关键词 Colon Epithelial Cells CXCR-4 IgA/IgG Chimeric Immunoglobulin Heterodimer COX-2 LGR-5 Musashi-1 Dedifferentiation Cellular Engraftment Oncoly-sis Gastrointestinal Progenitor Stem Cells (GIP-C)
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Deterioration of Dry Skin in Arthritis Model Mice via Stress-Induced Changes in Immune Cells in the Thymus and Spleen 认领
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作者 Kenji Goto Keiichi Hiramoto +1 位作者 Ion Takada Kazuya Ooi 《生物科学与医学(英文)》 2020年第1期23-37,共15页
Objective: Skin dryness is a characteristic of rheumatoid arthritis model mice. A previous study reported that the stress hormone glucocorticoid (i.e., corticosterone) is related to the induction of dry skin in arthri... Objective: Skin dryness is a characteristic of rheumatoid arthritis model mice. A previous study reported that the stress hormone glucocorticoid (i.e., corticosterone) is related to the induction of dry skin in arthritic mice. However, the mechanism through which stress induces dry skin in these mice is still unclear. Therefore, in this study, we examined the relationship between stress and induction of dry skin in arthritic mice. Methods: Physical stress load in mice with DBA/1JJmsSlc collagen-induced arthritis was treated with water immersion stress, and transepidermal water loss and the expression of markers associated with allergic reactions and inflammation was evaluated. Results: Deterioration of skin dryness was observed in stressed arthritic mice compared with that in unstressed arthritic mice. Moreover, plasma levels of interleukin-6 and corticosterone were increased in stressed arthritic mice compared with those in unstressed arthritic mice. We also observed decreased regulatory T cell numbers and increased T helper type 2 cell numbers in the thymus of stressed arthritic mice compared with those in unstressed arthritic mice. Conclusion: These results suggested that abnormalities in the immune system were related to deterioration of dry skin in stressed arthritic mice. Thus, reduction of stress may prevent deterioration of dry skin in mice with arthritis. 展开更多
关键词 ARTHRITIS Dry Skin STRESS HELPER T CELLS Regulatory T CELLS
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In-Host Analysis of Malaria Dynamics in Humans 认领
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作者 Kodwo Annan 《应用科学(英文)》 2020年第5期191-209,共19页
Human malaria infection poses a major global health threat worldwide. Yet, no sophisticated mathematical model exists to study the complex dynamics and interactions between the parasites and host immune response at th... Human malaria infection poses a major global health threat worldwide. Yet, no sophisticated mathematical model exists to study the complex dynamics and interactions between the parasites and host immune response at the blood and liver stages. In this paper, an in-host mathematical model of Plasmodium falciparum malaria dynamics and interactions in an infected host cells are studied at the liver stage by incorporating the red blood cells and the immune system. Numerical simulations are applied to investigate the interactions between the host immune response, the parasite dynamics, and the disease dynamics at both the blood and liver stages. Results show that immunity has a significant impact in clearing infected red blood cells. Furthermore, the infected erythrocytes and hence the severity of malaria tend to increase with increasing density of merozoites in the blood. The result revealed that intervention during malaria infection should focus on minimizing merozoite invasion rate on healthy erythrocytes and the density of merozoites in circulation. 展开更多
关键词 MALARIA Red BLOOD CELLS MATHEMATICAL Modeling MEROZOITE IMMUNE CELLS
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Current status and future prospects of stem cell therapy in Alzheimer’s disease 认领
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作者 Fu-Qiang Zhang Jin-Lan Jiang +3 位作者 Jing-Tian Zhang Han Niu Xue-Qi Fu Lin-Lin Zeng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期242-250,共9页
Alzheimer’s disease is a common progressive neurodegenerative disorder, pathologically characterized by the presence of β-amyloid plaques and neurofibrillary tangles. Current treatment approaches using drugs only al... Alzheimer’s disease is a common progressive neurodegenerative disorder, pathologically characterized by the presence of β-amyloid plaques and neurofibrillary tangles. Current treatment approaches using drugs only alleviate the symptoms without curing the disease, which is a serious issue and influences the quality of life of the patients and their caregivers. In recent years, stem cell technology has provided new insights into the treatment of neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. Currently, the main sources of stem cells include neural stem cells, embryonic stem cells, mesenchymal stem cells, and induced pluripotent stem cells. In this review, we discuss the pathophysiology and general treatment of Alzheimer’s disease, and the current state of stem cell transplantation in the treatment of Alzheimer’s disease. We also assess future challenges in the clinical application and drug development of stem cell transplantation as a treatment for Alzheimer’s disease. 展开更多
关键词 Alzheimer's disease β-amyloid drug development embryonic STEM CELLS induced PLURIPOTENT STEM CELLS mesenchymal STEM CELLS nerve REGENERATION NEURAL REGENERATION NEURAL STEM CELLS NEURODEGENERATIVE disorders STEM cell therapy
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Role of stem cell therapies in treating chronic wounds:A systematic review 认领
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作者 Anjali C Raghuram Roy P Yu +4 位作者 Andrea Y Lo Cynthia J Sung Melissa Bircan Holly J Thompson Alex K Wong 《世界干细胞杂志:英文版(电子版)》 SCIE CAS 2020年第7期659-675,共17页
BACKGROUND The impairment of cutaneous wound healing results in chronic,non-healing wounds that are caused by altered wound environment oxygenation,tissue injury,and permissive microbial growth.Current modalities for ... BACKGROUND The impairment of cutaneous wound healing results in chronic,non-healing wounds that are caused by altered wound environment oxygenation,tissue injury,and permissive microbial growth.Current modalities for the treatment of these wounds inadequately address the complex changes involved in chronic wound pathogenesis.Consequently,stem cell therapies have emerged as a potential therapeutic modality to promote cutaneous regeneration through trophic and paracrine activity.AIM To investigate current literature regarding use of stem cell therapies for the clinical treatment of chronic,non-healing wounds.METHODS PubMed,EMBASE,Cochrane Library,Web of Science,and Scopus were queried with combinations of the search terms“mesenchymal stem cells,”“adult stem cells,”“embryonic stem cells,”“erythroid precursor cells,”“stem cell therapies,”and“chronic wounds”in order to find relevant articles published between the years of 2000 and 2019 to review a 20-year experience.Reference lists from the articles were reviewed to identify additional pertinent articles.Retrieved manuscripts(reviews,case reports/series,retrospective/prospective studies,and clinical trials)were evaluated by the authors for their depiction of clinical stem cell therapy use.Data were extracted from the articles using a standardized collection tool.RESULTS A total of 43 articles describing the use of stem cell therapies for the treatment of chronic wounds were included in this review.While stem cell therapies have been explored in in vitro and in vivo applications in the past,recent efforts are geared towards assessing their clinical role.A review of the literature revealed that adipose-derived stem cells,bone marrow-derived stem cells,bone marrowderived mononuclear cells,epidermally-derived mesenchymal stem cells,fibroblast stem cells,keratinocyte stem cells,placental mesenchymal stem cells,and umbilical cord mesenchymal stem cells have all been employed in the treatment of chronic wounds of various etiologies.Most recently,embry 展开更多
关键词 Mesenchymal stem cells Adult stem cells Embryonic stem cells Erythroid precursor cells Stem cell therapies Chronic wounds
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Printing photovoltaics by electrospray 认领
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作者 Xinyan Zhao Weiwei Deng 《光电进展(英文)》 2020年第6期18-39,共22页
Solution processible photovoltaics(PV)are poised to play an important role in scalable manufacturing of low-cost solar cells.Electrospray is uniquely suited for fabricating PVs due to its several desirable characteris... Solution processible photovoltaics(PV)are poised to play an important role in scalable manufacturing of low-cost solar cells.Electrospray is uniquely suited for fabricating PVs due to its several desirable characteristics of an ideal manufacturing process such as compatibility with roll-to-roll production processes,tunability and uniformity of droplet size,capability of operating at atmospheric pressure,and negligible material waste and nano structures.This review begins with an introduction of the fundamentals and unique properties of electrospray.We put emphasis on the evaporation time and residence time that jointly affect the deposition outcome.Then we review the efforts of electrospray printing polymer solar cells,perovskite solar cells,and dye sensitized solar cells.Collectively,these results demonstrate the advantages of electrospray for solution processed PV.Electrospray has also exhibited the capability of producing uniform films as well as nanostructured and even multiscale films.So far,the electrospray has been found to improve active layer morphology,and create devices with efficiencies comparable with that of spin-coating.Finally,we discuss challenges and research opportunities that enable electrospray to become a mainstream technique for industrial scale production. 展开更多
关键词 photovoltaics ELECTROSPRAY solution process polymer solar cells perovskite solar cells dye sensitized solar cells
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Conjugated Polymers as Hole Transporting Materials for Solar Cells 认领
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作者 Dan Ti Kun Gao +1 位作者 Zhi-Pan Zhang Liang-Ti Qu 《高分子科学:英文版》 SCIE CAS CSCD 2020年第5期449-458,I0005,共11页
In principle,conjugated polymers can work as electron donors and thus as low-cost p-type organic semiconductors to transport holes in photovoltaic devices.With the booming interests in high-efficiency and low-cost sol... In principle,conjugated polymers can work as electron donors and thus as low-cost p-type organic semiconductors to transport holes in photovoltaic devices.With the booming interests in high-efficiency and low-cost solar cells to tackle global climate change and energy shortage,hole transporting materials(HTMs)based on conjugated polymers have received increasing attention in the past decade.In this perspective,recent advances in HTMs for a range of photovoltaic devices including dye-sensitized solar cells(DSSCs),perovskite solar cells(PSCs),and silicon(Si)/organic heterojunction solar cells(HSCs)are summarized and perspectives on their future development are also presented. 展开更多
关键词 Conjugated polymers Hole transporting materials Dye-sensitized solar cells Perovskite solar cells Silicon/organic heterojunction solar cells
Mesenchymal stromal cells as potential immunomodulatory players in severe acute respiratory distress syndrome induced by SARSCoV- 2 infection 认领
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作者 Panagiotis Mallis Efstathios Michalopoulos +1 位作者 Theofanis Chatzistamatiou Catherine Stavropoulos-Giokas 《世界干细胞杂志:英文版(电子版)》 SCIE CAS 2020年第8期731-751,共21页
Severe acute respiratory syndrome coronavirus-2 and the related coronavirus disease-19(COVID-19)is a worldwide emerging situation,which was initially reported in December 2019 in Wuhan,China.Currently,more than 725884... Severe acute respiratory syndrome coronavirus-2 and the related coronavirus disease-19(COVID-19)is a worldwide emerging situation,which was initially reported in December 2019 in Wuhan,China.Currently,more than 7258842 new cases,and more than 411879 deaths have been reported globally.This new highly transmitted coronavirus is responsible for the development of severe acute respiratory distress syndrome.Due to this disorder,a great number of patients are hospitalized in the intensive care unit followed by connection to extracorporeal membrane oxygenation for breath supporting and survival.Severe acute respiratory distress syndrome is mostly accompanied by the secretion of proinflammatory cytokines,including interleukin(IL)-2,IL-6,IL-7,granulocyte colony-stimulating factor(GSCF),interferon-inducible protein 10(IP10),monocyte chemotactic protein-1(MCP1),macrophage inflammatory protein 1A(MIP1A),and tumor necrosis factor alpha(TNF-α),an event which is known as“cytokine storm”.Further disease pathology involves a generalized modulation of immune responses,leading to fatal multiorgan failure.Currently,no specific treatment or vaccination against severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has been developed.Mesenchymal stromal cells(MSCs),which are known for their immunosuppressive actions,could be applied as an alternative co-therapy in critically-ill COVID-19 patients.Specifically,MSCs can regulate the immune responses through the conversion of Th1 to Th2,activation of M2 macrophages,and modulation of dendritic cells maturation.These key immunoregulatory properties of MSCs may be exerted either by produced soluble factors or by cell-cell contact interactions.To date,several clinical trials have been registered to assess the safety,efficacy,and therapeutic potential of MSCs in COVID-19.Moreover,MSC treatment may be effective for the reversion of ground-glass opacity of damaged lungs and reduce the tissue fibrosis.Taking into account the multifunctional properties of MSCs,the proposed stem-cell-base 展开更多
关键词 SARS-CoV-2 COVID-19 Respiratory syndrome Cytokine storm Mesenchymal stromal cells IMMUNOREGULATION Lungs Th2 response Dendritic cells Natural killer cells
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Recent progress of thin-film photovoltaics for indoor application 认领
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作者 Nanfu Yan Chaowei Zhao +2 位作者 Shengyong You Yuefeng Zhang Weiwei Li 《中国化学快报:英文版》 SCIE CAS CSCD 2020年第3期643-653,共11页
Indoor photovoltaics have attracted increasing attentions owing to their great potential in supplying energy for low power devices under indoor light in our daily life.The third generation thin-film solar cells,includ... Indoor photovoltaics have attracted increasing attentions owing to their great potential in supplying energy for low power devices under indoor light in our daily life.The third generation thin-film solar cells,including dye-sensitized solar cells,perovskite solar cells and organic solar cells,have made rapid progress from the aspect of materials design to photovoltaic performance.This review provides an overview on the recent advances in the development of indoor photovoltaic technologies based on the third generation solar cells.The design principles of advanced thin-film indoor photovoltaics were also summarized according to the characteristics of indoor light and the advantages of the third generation solar cells.Finally,after summarizing the current research progress,the perspective on this topic is provided. 展开更多
关键词 INDOOR photovoltaics DYE-SENSITIZED SOLAR CELLS Perovskites SOLAR CELLS ORGANIC SOLAR CELLS Power CONVERSION efficiency
GFAP expression in the optic nerve and increased H2S generation in the integration centers of the rainbow trout (Oncorhynchus mykiss) brain after unilateral eye injury 认领
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作者 Evgeniya V.Pushchina Anatoly A.Varaksin +1 位作者 Dmitry K.Obukhov Igor M.Prudnikov 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第10期1867-1886,共20页
Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular disorders.However,there are few reports on the effects of H2S in the central nervous system during stress or injury.Previous studies on ... Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular disorders.However,there are few reports on the effects of H2S in the central nervous system during stress or injury.Previous studies on goldfish have shown that astrocytic response occurs in the damaged and contralateral optic nerves.Glial fibrillary acidic protein(GFAP)concentration in the optic nerves of rainbow trout has not been measured previously.This study further characterized the astrocytic response in the optic nerve and the brain of a rainbow trout(Oncorhynchus mykiss)after unilateral eye injury and estimated the amount of H2S-producing enzyme cystathionineβ-synthase(CBS)in the brain of the rainbow trout.Within 1 week after unilateral eye injury,a protein band corresponding to a molecular weight of 50 kDa was identified in the ipsi-and contralateral optic nerves of the rainbow trout.The concentration of GFAP in the injured optic nerve increased compared to the protein concentration on the contralateral side.The results of a quantitative analysis of GFAP+cell distribution in the contralateral optic nerve showed the largest number of GFAP+cells and fibers in the optic nerve head.In the damaged optic nerve,patterns of GFAP+cell migration and large GFAP+bipolar activated astrocytes were detected at 1 week after unilateral eye injury.The study of H2S-producing system after unilateral eye injury in the rainbow trout was conducted using enzyme-linked immunosorbent assay,western blot analysis,and immunohistochemistry of polyclonal antibodies again st CBS in the integrative centers of the brain:telencephalon,optic tectum,and cerebellum.Enzyme-linked immunosorbent assay results showed a 1.7-fold increase in CBS expression in the rainbow trout brain at 1 week after unilateral eye injury compared with that in intact animals.In the ventricular and subventricular regions of the rainbow trout telencephalon,CBS+radial glia and neuroepithelial cells were identified.After unilateral eye injury,the number of CBS+neuroepithelial cells in the 展开更多
关键词 astrocyte-like CELLS GLIAL fibrillary acidic protein hydrogen sulfide NEUROEPITHELIAL CELLS neuroprotection radial GLIAL CELLS rainbow trout(Oncorhynchus mykiss) reactive oxygen species UNILATERAL eye injury
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Outgrowth endothelial cells form a functional cerebral barrier and restore its integrity after damage 认领
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作者 Rais Reskiawan Abdulkadir Mansour Alwjwaj +2 位作者 Othman Ahmad Othman Kamini Rakkar Ulvi Bayraktutan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第6期1071-1078,共8页
Breakdown of blood-brain barrier,formed mainly by brain microvascular endothelial cells(BMECs),represents the major cause of mortality during early phases of ischemic strokes.Hence,discovery of novel agents that can e... Breakdown of blood-brain barrier,formed mainly by brain microvascular endothelial cells(BMECs),represents the major cause of mortality during early phases of ischemic strokes.Hence,discovery of novel agents that can effectively replace dead or dying endothelial cells to restore blood-brain barrier integrity is of paramount importance in stroke medicine.Although endothelial progenitor cells(EPCs)represent one such agents,their rarity in peripheral blood severely limits their adequate isolation and therapeutic use for acute ischemic stroke which necessitate their ex vivo expansion and generate early EPCs and outgrowth endothelial cells(OECs)as a result.Functional analyses of these cells,in the present study,demonstrated that only OECs endocytosed DiI-labelled acetylated low-density lipoprotein and formed tubules on matrigel,prominent endothelial cell and angiogenesis markers,respectively.Further analyses by flow cytometry demonstrated that OECs expressed specific markers for sternness(CD34),immaturity(CD133)and endothelial cells(CD31)but not for hematopoietic cells(CD45).Like BMECs,OECs established an equally tight in vitro model of human BBB with astrocytes and pericytes,suggesting their capacity to form tight junctions.Ischemic injury mimicked by concurrent deprivation of oxygen and glucose(4 hours)or deprivation of oxygen and glucose followed by reperfusion(20 hours)affected both barrier integrity and function in a similar fashion as evidenced by decreases in transendothelial electrical resistance and increases in paracellular flux,respectively.Wound scratch assays comparing the vasculoreparative capacity of cells revealed that,compared to BMECs,OECs possessed a greater proliferative and directional migratory capacity.In a triple culture model of BBB established with astrocytes,pericytes and BMEC,exogenous addition of OECs effectively repaired the damage induced on endothelial layer in serum-free conditions.Taken together,these data demonstrate that OECs may effectively home to the site of vascular injury and re 展开更多
关键词 cell-based therapy ENDOTHELIAL progenitor CELLS ENDOTHELIUM ischemic stroke neurodegeneration novel therapeutics OUTGROWTH ENDOTHELIAL CELLS regenerative MEDICINE stem CELLS translational MEDICINE
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Transfer of mitochondria from mesenchymal stem cells derived from induced pluripotent stem cells attenuates hypoxia-ischemia-induced mitochondrial dysfunction in PC12 cells 认领
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作者 Yan Yang Gen Ye +5 位作者 Yue-Lin Zhang Hai-Wei He Bao-Qi Yu Yi-Mei Hong Wei You Xin Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期464-472,共9页
Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury.Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology,the mechanisms are not fully... Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury.Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology,the mechanisms are not fully understood.To address this issue,we first co-cultured 1.5×10^5 PC12 cells with mesenchymal stem cells that were derived from induced pluripotent stem cells at a ratio of 1:1,and then intervened with cobalt chloride(CoCl2)for 24 hours.Reactive oxygen species in PC12 cells was measured by Mito-sox.Mitochondrial membrane potential(ΔΨm)in PC12 cells was determined by JC-1 staining.Apoptosis of PC12 cells was detected by terminal deoxynucleotidal transferase-mediated dUTP nick end-labeling staining.Mitochondrial morphology in PC12 cells was examined by transmission electron microscopy.Transfer of mitochondria from the mesenchymal stem cells derived from induced pluripotent stem cells to damaged PC12 cells was measured by flow cytometry.Mesenchymal stem cells were induced from pluripotent stem cells by lentivirus infection containing green fluorescent protein in mitochondria.Then they were co-cultured with PC12 cells in Transwell chambers and treated with CoCl2 for 24 hours to detect adenosine triphosphate level in PC12 cells.CoCl2-induced PC12 cell damage was dose-dependent.Co-culture with mesenchymal stem cells significantly reduced apoptosis and restoredΔΨm in the injured PC12 cells under CoCl2 challenge.Co-culture with mesenchymal stem cells ameliorated mitochondrial swelling,the disappearance of cristae,and chromatin margination in the injured PC12 cells.After direct co-culture,mitochondrial transfer from the mesenchymal stem cells stem cells to PC12 cells was detected via formed tunneling nanotubes between these two types of cells.The transfer efficiency was greatly enhanced in the presence of CoCl2.More importantly,inhibition of tunneling nanotubes partially abrogated the beneficial effects of mesenchymal stem cells on CoCl2-induced PC12 cell injury.Mesenchymal stem cells reduced CoCl2-induced 展开更多
关键词 apoptosis brain injury HYPOXIA-ISCHEMIA INDUCED pluripotent STEM CELLS mesenchymal STEM CELLS MITOCHONDRIAL membrane potential MITOCHONDRIAL TRANSFER PC12 CELLS tunneling nanotubes
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Insights into stem cell therapy for diabetic retinopathy: a bibliometric and visual analysis 认领
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作者 Xiang-Jun Li Chun-Yan Li +1 位作者 Dan Bai Ying Leng 《中国神经再生研究:英文版》 SCIE CAS 2021年第1期172-178,共7页
Stem cells have been confirmed to be involved in the occurrence and development of diabetic retinopathy;however,the underlying mechanisms remain unclear.In this study,we used Citespace software to visually analyze 552... Stem cells have been confirmed to be involved in the occurrence and development of diabetic retinopathy;however,the underlying mechanisms remain unclear.In this study,we used Citespace software to visually analyze 552 articles exploring the stem cell-based treatment of diabetic retinopathy over the past 20 years,which were included in the Web of Science Core Collection.We found the following:(1)a co-citation analysis of the references cited by all 552 articles indicated 15 clusters.In cluster#0,representing the stem cell field,some highly cited landmark studies emerged between 2009–2013.For example,endothelial progenitor cells and diabetic retinopathy gradually received the full attention of scholars,in terms of their relationship and therapeutic prospects.Some researchers also verified the potential of adipose-derived stem cells to differentiate into stable retinal perivascular cells,using a variety of animal models of retinal vascular disease.All of these achievements provided references for the subsequent stem cell research.(2)An analysis of popular keywords among the 552 articles revealed that,during the past 20 years,a relative increase in basic research articles examining stem cells and endothelial progenitor cells for the treatment of diabetic retinopathy was observed.The contents of these articles primarily involved the expression of vascular endothelial growth factor,vascular regeneration,oxidative stress,and inflammatory response.(3)A burst analysis of keywords used in the 552 articles indicated that genetic and cytological research regarding the promotion of angiogenesis was an issue of concern from 2001 to 2012,including several studies addressing the expression of various growth factor genes;from 2014 to 2020,mouse models of diabetic retinopathy were recognized as mature animal models,and the most recent research has focused on macular degeneration,macular edema,neurodegeneration,and inflammatory changes in diabetic animal models.(4)Globally,the current authoritative studies have focused on basic r 展开更多
关键词 DIABETES diabetic retinopathy epithelial cells MACULA progenitor cells RETINA stem cells visual analysis
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文章速递Neurons derived from human-induced pluripotent stem cells express mu and kappa opioid receptors 认领
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作者 Zhi-Hai Ju Xuan Liang +3 位作者 Yao-Yao Ren Luo-Wa Shu Yan-Hong Yan Xu Cui 《中国神经再生研究:英文版》 SCIE CAS CSCD 2021年第4期653-658,共6页
Neuroprotection studies have shown that induced pluripotent stem(iPS)cells have the possibility to transform neuroprotection research.In the present study,iPS cells were generated from human renal epithelial cells and... Neuroprotection studies have shown that induced pluripotent stem(iPS)cells have the possibility to transform neuroprotection research.In the present study,iPS cells were generated from human renal epithelial cells and were then differentiated into neurons.Cells in the iPScell group were maintained in stem cell medium.In contrast,cells in the iPS-neuron group were first maintained in neural induction medium and expansion medium containing ROCK inhibitors,and then cultivated in neuronal differentiation medium and neuronal maturation medium to induce the neural stem cells to differentiate into neurons.The expression of relevant markers was compared at different stages of differentiation.Immunofluorescence staining revealed that cells in the iPS-neuron group expressed the neural stem cell markers SOX1 and nestin on day 11 of induction,and neuronal markers TUBB3 and NeuN on day 21 of induction.Polymerase chain reaction results demonstrated that,compared with the iPS-cell group,TUBB3 gene expression in the iPS-neuron group was increased 15.6-fold.Further research revealed that,compared with the iPS-cell group,the gene expression and immunoreactivity of mu opioid receptor in the iPS-neuron group were significantly increased(38.3-fold and 5.7-fold,respectively),but those of kappa opioid receptor had only a slight change(1.33-fold and 1.57-fold increases,respectively).Together,these data indicate that human iPS cells can be induced into mu opioid receptor-and kappa opioid receptor-expressing neurons,and that they may be useful to simulate human opioid receptor function in vitro and explore the underlying mechanisms of human conditions. 展开更多
关键词 brain cells factor in vivo neural differentiation protein stem cells stroke
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文章速递Biosafety of a 3D-printed intraocular lens made of a poly (acrylamide-co-sodium acrylate) hydrogel in vitro and in vivo 认领
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作者 Jia-Wen Li Yi-Jian Li +4 位作者 Xi-Su Hu Yu Gong Ben-Bin Xu Hai-Wei Xu Zheng-Qin Yin 《国际眼科杂志:英文版》 SCIE CAS 2020年第10期1521-1530,共10页
AIM:To assess the biosafety of a poly(acrylamide-cosodium acrylate)hydrogel(PAH)as a 3D-printed intraocular lens(IOL)material.METHODS:The biosafety of PAH was first evaluated in vitro using human lens epithelial cells... AIM:To assess the biosafety of a poly(acrylamide-cosodium acrylate)hydrogel(PAH)as a 3D-printed intraocular lens(IOL)material.METHODS:The biosafety of PAH was first evaluated in vitro using human lens epithelial cells(LECs)and the ARPE19 cell line,and a cell counting kit-8(CCK-8)assay was performed to investigate alterations in cell proliferation.A thin film of PAH and a conventional IOL were intraocularly implanted into the eyes of New Zealand white rabbits respectively,and a sham surgery served as control group.The anterior segment photographs,intraocular pressure(IOP),blood parameters and electroretinograms(ERG)were recorded.Inflammatory cytokines in the aqueous humor,such as TNFαand IL-8,were examined by ELISA.Cell apoptosis of the retina was investigated by TUNEL assay,and macro PAHge activation was detected by immunostaining.RESULTS:PAH did not slow cell proliferation when cocultured with human LECs or ARPE19 cells.The implantation of a thin film of a 3 D-printed IOL composed of PAH did not affect the IOP,blood parameters,ERG or optical structure in any of the three experimental groups(n=3 for each).Both TNFαand IL-8 in the aqueous humor of PAH group were transiently elevated 1 wk post-operation and recovered to normal levels at 1 and 3 mo post-operation.Iba1+macroPAHges in the anterior chamber angle in PAH group were increased markedly compared to those of the control group;however,there was no significant difference compared to those in the IOL group.CONCLUSION:PAH is a safe material for 3D printing of personal IOLs that hold great potential for future clinical applications. 展开更多
关键词 cataract biosafety lens epithelial cells ARPE19 cells intraocular lens 3D printing poly hydrogel rabbit
文章速递Progress Update in Space Cell Mechano-biological Coupling 认领
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作者 LONG Mian SUN Shujin +2 位作者 LI Ning LÜDongyuan GAO Yuxin 《空间科学学报》 CAS CSCD 北大核心 2020年第5期935-936,共2页
Recent progresses in 2018–2019 from space experiments onboard SJ-10 recoverable satellite and on parabolic flight were summarized,mainly focusing on cell mechano-biological coupling under microgravity.In the meantime... Recent progresses in 2018–2019 from space experiments onboard SJ-10 recoverable satellite and on parabolic flight were summarized,mainly focusing on cell mechano-biological coupling under microgravity.In the meantime,technical pre-research and experimental system design for the biomechanics research platform on China Space Station was carried out and updated. 展开更多
关键词 Microgravity SJ-10 satellite Parabolic flight Endothelial cells Mesenchymal stem cells Hepatocytes Mechanotransduction China Space Station
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文章速递Secondary release of the peripheral nerve with autologous fat derivates benefits for functional and sensory recovery 认领
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作者 Natalia E.Krzesniak Anna Sarnowska +3 位作者 Anna Figiel-Dabrowska Katarzyna Osiak Krystyna Domanska-Janik Bartłomiej H.Noszczyk 《中国神经再生研究:英文版》 SCIE CAS CSCD 2021年第5期856-864,共9页
The reconstruction of nerve continuity after traumatic nerve injury is the gold standard in hand surgery.Immediate,tension-free,end-to-end nerve suture ensures the best prognosis.The recovery is mostly promising;howev... The reconstruction of nerve continuity after traumatic nerve injury is the gold standard in hand surgery.Immediate,tension-free,end-to-end nerve suture ensures the best prognosis.The recovery is mostly promising;however,in a few cases,insufficient outcomes in motor or sensory function are observed.Intra-and extra-fascicular scarring accompanies the nerve regeneration process and limits final outcomes.Secondary nerve release in those cases is recommended.Unfortunately,scarring recurrence cannot be eliminated after secondary revision and neurolysis.The supportive influences of mesenchymal stem cells in the process of nerve regeneration were observed in many preclinical studies.However,a limited number of studies in humans have analyzed the clinical usage of mesenchymal stem cells in peripheral nerve reconstruction and revisions.The objective of this study was to evaluate the effects of undifferentiated adipose-derived stromal/stem cell injection during a last-chance surgery(neurolysis,nerve release)on a previously reconstructed nerve.Three patients(one female,two males;mean age 59±4.5 years at the time of injury),who experienced failure of reconstructions of median and ulnar nerves,were included in this study.During the revision surgery,nerve fascicles were released,and adiposederived stromal/stem cells were administered through microinjections along the fascicles and around the adjacent tissues after external neurolysis.During 36 months of followup,patients noticed gradual signs of sensory and in consequence functional recovery.No adverse effects were observed.Simultaneous nerve release with adipose-derived stromal/stem cells support is a promising method in patients who need secondary nerve release after nerve reconstruction.This method can constitute an alternative procedure in patients experiencing recovery failure and allow improvement in cases of limited nerve regeneration.The study protocol was approved by the Institutional Review Board(IRB)at the Centre of Postgraduate Medical Education(No.62/PB/2016)on September 14,2016. 展开更多
关键词 nerve release neurolysis stem cells adipose-derived stem cells scar threshold sensation pain hypersensitivity forearm
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文章速递Inflammatory bowel disease:Therapeutic limitations and prospective of the stem cell therapy 认领
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作者 Rangnath Mishra Punita Dhawan +1 位作者 Anand S Srivastava Amar B Singh 《世界干细胞杂志:英文版(电子版)》 SCIE CAS 2020年第10期1050-1066,共17页
Inflammatory bowel disease(IBD),consisting primarily of ulcerative colitis and Crohn’s disease,is a group of debilitating auto-immune disorders,which also increases the risk of colitis-associated cancer.However,due t... Inflammatory bowel disease(IBD),consisting primarily of ulcerative colitis and Crohn’s disease,is a group of debilitating auto-immune disorders,which also increases the risk of colitis-associated cancer.However,due to the chronic nature of the disease and inconsistent treatment outcomes of current anti-IBD drugs(e.g.,approximately 30%non-responders to anti-TNFαagents),and related serious side effects,about half of all IBD patients(in millions)turn to alternative treatment options.In this regard,mucosal healing is gaining acceptance as a measure of disease activity in IBD patients as recent studies have correlated the success of mucosal healing with improved prognosis.However,despite the increasing clinical realization of the significance of the concept of mucosal healing,its regulation and means of therapeutic targeting remain largely unclear.Here,stemcell therapy,which uses hematopoietic stem cells or mesenchymal stem cells,remains a promising option.Stem cells are the pluripotent cells with ability to differentiate into the epithelial and/or immune-modulatory cells.The overreaching concept is that the stem cells can migrate to the damaged areas of the intestine to provide curative help in the mucosal healing process.Moreover,by differentiating into the mature intestinal epithelial cells,the stem cells also help in restoring the barrier integrity of the intestinal lining and hence prevent the immunomodulatory induction,the root cause of the IBD.In this article,we elaborate upon the current status of the clinical management of IBD and potential role of the stem cell therapy in improving IBD therapy and patient’s quality of life. 展开更多
关键词 Crohn′s disease Ulcerative colitis Mesenchymal stem cells Hematopoietic stem cells Mucosal healing
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