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Effects of Low Temperature and Low Light on Physiology of Tomato Seedlings 认领
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作者 Yubo Yang Lingdi Dong +5 位作者 Linqi Shi Jinghua Guo Yonggang Jiao Haizheng Xiong Ryan William Dickson Ainong Shi 《美国植物学期刊(英文)》 2020年第2期162-179,共18页
The physiological changes and the mechanism of stress tolerance in tomato were studied under low temperature and low light conditions. Two growth chamber experiments evaluated three temperatures regimes under standard... The physiological changes and the mechanism of stress tolerance in tomato were studied under low temperature and low light conditions. Two growth chamber experiments evaluated three temperatures regimes under standard and relatively low illumination levels with three tomato genotypes. Both experiments used a completely randomized split-plot design (CRD), with temperature regime as the main plot and tomato genotype as the split-plot. The three tomato varieties were “Fenyan No.1”, “SV0313TG”, and “Ousa”. In both experiments, activity of superoxide dismutase (SOD) and peroxidases (POD) in tomato seedlings decreased under low temperature regime and the combination of low temperature and low light. Decreasing temperature had the greatest effect on the increase in enzyme activity. Decrease in POD activity was the greatest under low light and low temperature. The concentration of malondialdehyde (MDA) in plant tissue also decreased under low temperature (20°C/10°C day/night) compared to the standard temperature control (25°C/16°C day/night), but increased at 15°C/5°C day/night temperatures in both experiments and was the greatest under the lowest light and temperature conditions. In both experiments, proline concentrations were the greatest under the standard light intensity (30,000 lux), and proline concentrations increased as temperature decreased. The content of soluble sugar decreased under only low temperature stress but increased under double stresses. The relative value of osmotic potential increased a little under low temperature stress but decreased under double stresses. 展开更多
关键词 Enzyme Activity MALONDIALDEHYDE OSMOTIC Potential PEROXIDASES PROLINE Physiological Condition Soluble Sugar Stress Tolerance Superoxide DISMUTASE
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Structure and function of a novel cold regulated cold shock domain containing protein from an obligate psychrophilic yeast,Glaciozyma antarctica 认领
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作者 Jennifer CHARLES Makdi MASNODDIN +1 位作者 Farhan NAZAIE Nur Athirah YUSOF 《极地科学进展:英文版》 CSCD 2020年第2期137-145,共9页
Cold shock domain(CSD)-containing proteins are one of the groups of the evolutionarily conserved nucleic acid-binding proteins in all three domains of life consisting of an ancient beta-barrel fold that serves to bind... Cold shock domain(CSD)-containing proteins are one of the groups of the evolutionarily conserved nucleic acid-binding proteins in all three domains of life consisting of an ancient beta-barrel fold that serves to bind nucleic acids.The c DNA of a novel protein-coding gene containing CSD was cloned from Glaciozyma antarctica designated as Ga16676.The full length of Ga16676 gene with the size of 1335 bp encodes for an N-terminal CSD with conserved nucleic acids binding motif RNP1 and RNP2.The Ga16676 gene was cloned in p ET30 Ek/LIC,sequenced,expressed and its resistance towards cold was characterized.Recombinant protein expression of Ga16676 showed overexpressed soluble expression in both supernatant and pellet forms at 20℃.The effects of recombinant CSD protein overexpression on colony formation shows that E.coli cells were able to grow at 37℃and 20℃but not at 4℃while E.coli_Ga16676 cells were able to grow at all temperatures tested.In addition,E.coli_Ga16676 cells showed higher growth rate compared to empty E.coli cells at 10℃.Structural analysis of Ga16676 reveals some interesting findings such as more aromatic interactions for efficient binding in low energy environment,a longer loop that may contribute to structural flexibility and clustering of charged amino acids on the protein surface that is important for protein stability and flexibility. 展开更多
关键词 cold shock protein(CSP) cold adaptation cold stress stress response stress tolerance Glaciozyma antarctica psychrophilic yeast
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耐盐胁迫相关离子转运蛋白与作物耐盐育种研究的进展 认领
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作者 Lu HUANG De-zhi WU Guo-ping ZHANG 《浙江大学学报:B卷英文版》 SCIE CAS CSCD 2020年第6期426-441,共16页
植物组织特别是地上部钠离子的积累是影响耐盐性的关键因素,这一过程涉及到一些离子转运蛋白,如SOS1、HKT和NHX等。不同植物种和同一物种不同品种之间的耐盐性差异很大,这种差异与耐盐胁迫相关离子转运蛋白基因表达有关。分子标记辅助... 植物组织特别是地上部钠离子的积累是影响耐盐性的关键因素,这一过程涉及到一些离子转运蛋白,如SOS1、HKT和NHX等。不同植物种和同一物种不同品种之间的耐盐性差异很大,这种差异与耐盐胁迫相关离子转运蛋白基因表达有关。分子标记辅助育种、诱变技术、遗传转化和基因编辑技术为耐盐作物育种提供了高效有力的技术支撑,特别是迅速发展的基因编辑技术,将从非转基因的技术手段有效地改良作物的耐盐性。 展开更多
关键词 盐害 渗透胁迫 离子胁迫