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Production of bio-fertilizer from Ascophyllum nodosum and Sargassum muticum(Phaeophyceae) 预览
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作者 SILVA Luís Daniel BAHCEVANDZIEV Kiril PEREIRA Leonel 《海洋湖沼学报(英文)》 SCIE CAS CSCD 2019年第3期918-927,共10页
Oceans and seas form a large body of water that contains a natural biodiversity.For humans,represents a resource,which makes this a point of interest,from researches to improve the economy.Seaweeds produce many compou... Oceans and seas form a large body of water that contains a natural biodiversity.For humans,represents a resource,which makes this a point of interest,from researches to improve the economy.Seaweeds produce many compounds and secondary metabolites that can be used in different fi elds of industry such as food,agricultural,pharmaceutical and health.Even though seaweeds are ancestral resources,recently it was notorious a global interest in knowing more about its potentials,where biotechnology plays an important role in research.Studies showed that seaweed has many bioactive compounds benefi cial to plant development,giving them a great potential as an agricultural fertilizer.Adding seaweeds to the soil provides organic matter,minerals,trace elements,growth plant regulator,metabolites,vitamins,and amino acids and it can work as a soil conditioner.In Portugal,the use of seaweeds for agriculture is important since long time ago.In the past,populations that lived near coastal zone depended on the seaweeds as a family subsistence but,throughout the years,synthetic fertilizers replaced seaweeds.Our work aimed to assess the potential of the extracts obtained from Ascophyllum nodosum and from Sargassum muticum as an agricultural fertilizer.This evaluation was carried out with rice plants(Oryza sativa)and lettuce(Lactuca sativa),in germination bioassays,the culture of rice and lettuce plants in pots,and culture of lettuce plants in hydroponics.For that,seaweed liquid extracts were used in different concentrations in different bioassays.Results show that extracts obtained from two seaweeds,A.nodosum and S.muticum,can be promissory plant biofertilizer at a concentration of 25% and had a positive effect on seed germination,plant development,and production. 展开更多
关键词 FERTILIZER bioactive compounds Ascophyllum nodosum SARGASSUM muticum Oryza SATIVA LACTUCA SATIVA
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Protective Effects of Tribulus terrestris,Avena sativa,and White Ginseng Powder on Bone Mineral Density in Hypercholesterolemic Rats 预览
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作者 Mehmet Akdogan Huri Tilla Ilce +1 位作者 Ali Bilgili Basak Hanedan 《药剂与药理学:英文版》 2019年第6期286-292,共7页
This study investigated the effects of the herbal compounds Tribulus terrestris(TT),Avena sativa(AS),white ginseng(WG),and a triple combination(TC)powder on the serum total cholesterol,low-density lipoprotein choleste... This study investigated the effects of the herbal compounds Tribulus terrestris(TT),Avena sativa(AS),white ginseng(WG),and a triple combination(TC)powder on the serum total cholesterol,low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),triglyceride,and the tibial bone mineral density(BMD)levels in rats fed a high-cholesterol diet.This study comprised Group I(normal pellet feed),Group II(pellet feed with 2%cholesterol),Group III(2%cholesterol plus 15%TT),Group IV(2%cholesterol plus 7.5%AS),Group V(2%cholesterol plus 5%WG)and Group VI(2%cholesterol plus 7.5 TT%+3.75%AS,2.5%WG).The serum total cholesterol,LDL-C,HDL-C,triglyceride,and tibial BMD(g/cm2)levels were measured.Significant decrease in the serum HDL-C levels in Group II than Group I,and significant increase in the serum LDL-C levels in Group II than Groups I and VI were determined.The tibial BMD levels were significantly lower in Groups II and IV than Group I.It was determined that WG and TC significantly prevented the serum total cholesterol increase;TC significantly prevented the serum LDL-C increase;and TT,WG,and TC were non-significantly effective in the improvement of tibial BMD levels. 展开更多
关键词 LIPID and LIPOPROTEINS Tribulus terrestris AVENA SATIVA WHITE GINSENG bone mineral density
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Postharvest responses of hydroponically grown lettuce varieties to nitrogen application rate 预览
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作者 Bevly M.Mampholo Martin Maboko +1 位作者 Puffy Soundy Dharini Sivakumar 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第10期2272-2283,共12页
Limited information is available on the influence of preharvest N application rates on postharvest quality of different lettuce genotypes. Two green leafy lettuce (Multigreen 1 and Multigreen 3) and red leafy lettuce ... Limited information is available on the influence of preharvest N application rates on postharvest quality of different lettuce genotypes. Two green leafy lettuce (Multigreen 1 and Multigreen 3) and red leafy lettuce (Multired 4) were grown in gravel film technique and fertigated with five different N application rates: 60, 90, 120, 150 and 180 mg L^-1. The 120 mg L^-1 N application is commercially recommended for lettuce. After harvest, lettuce samples were packed in a bioriented poly propylene packaging (5% O2 and 5% CO2) and held at 5°C and 85% RH for 3, 6, 9 and 12 days. The genotypes, preharvest N application rates and storage time affected the leaf colour coordinates, phenolic acids (dicaffeoyltataric acid, caffeoyl tartaric acid, 3-caffeoylquinic acid and 3,4-dihydroxycinnamic acid) and browning enzyme activities (phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD)). Lower rates of N application at preharvest stage showed higher weight loss with the storage time increasing in Multigreen 3. In Multigreen 1, colour coordinate b* value decreased remarkably with N application rates from 60 to 120 mg L^–1 due to the onset of browning during storage. While in Multigreen 3 and N application higher than 60 mg L^–1 influenced the decrease in b* value. Browning occurred due to the increased activity of PAL enzyme and the availability of the substrates caftaric, chlorogenic, caffeic acids, PPO activity and production of browning pigments due to the activity of POD. Higher, N application rates (>120 mg L^–1) influenced the browning mechanism and showed brownish red leaves in Multired 4 during storage. Higher ascorbic acid concentration played a role in reducing the onset of browning in the fresh cuts leaves of Mulitired 4 and Multigreen 3 fertilized with lower preharvest lower N application rates (<120 mg L^–1). Preharvest N application at 90 mg L^–1 retained the colour, ascorbic acid content and the phenolic acid components and extended the shelf life of Multired 4 lettuce up t 展开更多
关键词 LACTUCA sativa ascorbic acid PHENYLALANINE ammonia-lyase POLYPHENOL oxidase tissue BROWNING
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OsBGLU19 and OsBGLU23 regulate disease resistance to bacterial leaf streak in rice 预览
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作者 LI Bei-bei LIU Ying-gao +4 位作者 WU Tao WANG Ji-peng XIE Gui-rong CHU Zhao-hui DING Xin-hua 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第6期1199-1210,共12页
β-Glucosidase belongs to the glycoside hydrolase I family,which is widely present in multiple species and responds to various biotic and abiotic stresses.In rice,whetherβ-glucosidase is involved in the interaction b... β-Glucosidase belongs to the glycoside hydrolase I family,which is widely present in multiple species and responds to various biotic and abiotic stresses.In rice,whetherβ-glucosidase is involved in the interaction between plants and microorganisms is not clear.In this study,we found that the expression of several genes encodingβ-glucosidases,including OsBGLU19 and OsBGLU23,were upregulated after inoculation with Xanthomonas oryzae pv.oryzicola(Xoc)and downregulated after inoculation with X.oryzae pv.oryzae(Xoo).The respective insertion mutants of OsBGLU19 and OsBGLU23,bglu19 and bglu23,were more susceptible to Xoc infection.The expression of OsAOS2,a key gene in the jasmonic acid signal pathway,was dramatically downregulated after inoculation with Xoc in the bglu19 and bglu23 mutants.Simultaneously,the expression of downstream disease resistance-related genes,such as OsPR1a,OsPR5 and a key transcription factors OsWRKY72 were obviously downregulated.The resistance mediated by OsBGLU19 and OsBGLU23 to bacterial leaf streak is related to disease resistance-related genes above mentioned. 展开更多
关键词 Β-GLUCOSIDASE OsBGLU19 OsBGLU23 ORYZA SATIVA bacterial leaf STREAK
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广谱抗稻瘟病基因Pi9的共显性功能标记的开发与利用
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作者 李永聪 匡博文 +5 位作者 周小龙 刘芝妤 林晋军 肖应辉 刘雄伦 刘金灵 《分子植物育种》 CAS CSCD 北大核心 2019年第16期5342-5346,共5页
稻瘟病是水稻最严重的真菌病害之一。利用抗病基因培育抗病品种是防治稻瘟病最有效的策略。抗稻瘟病基因Pi9是第一个克隆的广谱抗稻瘟病基因,对世界多个国家和地区的稻瘟菌小种都表现高水平抗性,有着重要的应用价值。为了针对拟改良的... 稻瘟病是水稻最严重的真菌病害之一。利用抗病基因培育抗病品种是防治稻瘟病最有效的策略。抗稻瘟病基因Pi9是第一个克隆的广谱抗稻瘟病基因,对世界多个国家和地区的稻瘟菌小种都表现高水平抗性,有着重要的应用价值。为了针对拟改良的不同遗传背景的水稻材料,开发Pi9基因特异性功能分子标记,用于分子标记辅助选择育种,本研究开发设计了1个新的Pi9基因共显性功能分子标记。通过对23份育种核心材料的多态性检测和育种改良杂交F1代、BC6F2代群体的鉴定,表明该分子标记能有效的对Pi9功能基因进行鉴定,并对回交育种后代进行高效选择,为培育Pi9基因抗病品种提供了新的分子标记辅助选择技术。 展开更多
关键词 水稻(Oryza sativa) 稻瘟病 共显性功能 Pi9
Physiological and genome-wide gene expression analyses of cold-induced leaf rolling at the seedling stage in rice (Oryza sativa L.) 预览
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作者 Jingfang Dong Junliang Zhao +6 位作者 Shaohong Zhang Tifeng Yang Qing Liu Xingxue Mao Hua Fu Wu Yang Bin Liu 《作物学报:英文版》 CAS CSCD 2019年第4期431-443,共13页
Leaf rolling and discoloration are two chilling-injury symptoms that are widely used as indicators for the evaluation of cold tolerance at the seedling stage in rice. However, the difference in cold-response mechanism... Leaf rolling and discoloration are two chilling-injury symptoms that are widely used as indicators for the evaluation of cold tolerance at the seedling stage in rice. However, the difference in cold-response mechanisms underlying these two traits remains unknown. In the present study, a cold-tolerant rice cultivar, Lijiangxintuanheigu, and a cold-sensitive cultivar, Sanhuangzhan-2, were subjected to low-temperature treatments and physiolog-ical and genome-wide gene expression analyses were conducted. Leaf rolling occurred at temperatures lower than 11℃, whereas discoloration appeared at moderately low temperatures such as 13℃. Chlorophyll contents in both cultivars were significantly decreased at 13℃, but not altered at 11℃. In contrast, the relative water content and relative electrolyte leakage of both cultivars decreased significantly at 11℃, but did not change at 13℃. Expression of genes associated with calcium signaling and abscisic acid (ABA) degradation was significantly altered at 11℃ in comparison with 25℃ and 13℃. Numerous genes in the DREB, MYB, bZIP, NAC, Zinc finger, bHLH, and WRKY gene families were differentially expressed. Many aquaporin genes and the key genes in trehalose and starch synthesis were down regulated at 11℃ in comparison with 25℃ and 13℃. These results suggest that the two chilling injury symptoms are temperature-specific and are controlled by different mechanisms. Cold-induced leaf rolling is associated with calcium and ABA signaling pathways and is regulated by multiple transcriptional regulators. The suppression of aquaporin genes and reduced accumulation of soluble sugars under cold stress results in a reduction in cellular water potential and consequently leaf rolling. 展开更多
关键词 Rice(Oryza sativa L.) Cold-induced LEAF yellowing Cold-induced LEAF rolling PHYSIOLOGICAL analysis GENOME-WIDE expression profiling
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Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice
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作者 Bin Ren Lang Liu +6 位作者 Shaofang Li Yongjie Kuang Jingwen Wang Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou 《分子植物:英文版》 SCIE CAS CSCD 2019年第7期1015-1026,共12页
CRISPR technologies enabling precise genome manipulation are valuable for gene function studies and molecular crop breeding. However, the requirement of a protospacer adjacent motif (PAM)y such as NGG and TTN, for Cas... CRISPR technologies enabling precise genome manipulation are valuable for gene function studies and molecular crop breeding. However, the requirement of a protospacer adjacent motif (PAM)y such as NGG and TTN, for Cas protein recognition restricts the selection of targetable genomic loci in practical applications of CRISPR technologies. Recently Cas9-NG, which recognizes a minimal NG PAM, was reported to expand the targeting space of genome editing in human cells, but it remains unclear whether this Cas9 variant can be used in plants. In this study, we evaluated the nuclease activity of Cas9-NG toward various NGN PAMs by targeting endogenous genes in transgenic rice. We found that Cas9-NG edits all NGG, NGA, NGT, and NGC sites with impaired activity, while the gene-edited plants were dominated by monoallelic mutations. Cas9-NG-engineered base editors were then developed and used to generate O s B Z R I gainof- function plants that can not be created by other available Cas9-engineered base editors. Moreover, we showed that a Cas9-NG-based transcriptional activator efficiently upregulated the expression of endogenous target genes in rice. In addition, we discovered that Cas9-NG recognizes NAC, NTG, NTT, and NCG apart from NG PAM. Together, these findings demonstrate that Cas9-NG can greatly expand the targeting scope of genome-editing tools, showing great potential for targeted genome editing, base editing, and genome regulation in plants. 展开更多
关键词 CRISPR Cas9-NG PAM gene EDITING base EDITING ORYZA SATIVA L
Wx^lv、the Ancestral Allele of Rice Waxy Gene
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作者 Changquan Zhang Jihui Zhu +9 位作者 Shengjie Chen Xiaolei Fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu Chuandeng Yi Shuzhu Tang Minghong Gu Qiaoquan Liu 《分子植物:英文版》 SCIE CAS CSCD 2019年第8期1157-1166,共10页
In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking quality.The effects of several Wx alleles on amylose content and the taste of cooked ... In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking quality.The effects of several Wx alleles on amylose content and the taste of cooked rice have been elucidated.However,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain unclear.Here we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose molecules.We demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional sites.These data indicate that the Wx^lv allele played an important role in artificial selection and domestication.The findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice. 展开更多
关键词 ORYZA SATIVA WAXY EATING and COOKING quality allelic variation artificial selection
水稻稻瘟病抗性研究与展望 预览
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作者 赵夏夏 王旭明 +6 位作者 许飘 赵丽娜 胡燕 陈景阳 黄永相 李伟 郭建夫 《湖北农业科学》 2019年第11期5-9,共5页
稻瘟病是造成水稻(Oryza sativa L.)减产危害最严重的病害之一。为了发掘、鉴定和筛选水稻抗性种质资源,有效利用抗病的水稻品种来抑制稻瘟病的发生,解决水稻生产的障碍并掌握抗性种质资源的抗瘟基因及其分布,从水稻抗稻瘟病基因的抗性... 稻瘟病是造成水稻(Oryza sativa L.)减产危害最严重的病害之一。为了发掘、鉴定和筛选水稻抗性种质资源,有效利用抗病的水稻品种来抑制稻瘟病的发生,解决水稻生产的障碍并掌握抗性种质资源的抗瘟基因及其分布,从水稻抗稻瘟病基因的抗性鉴定、抗性资源的筛选与利用、抗性遗传规律及抗病基因定位4个方面,对水稻稻瘟病抗性的研究进展进行了综述,为水稻抗稻瘟病资源的收集、选育和抗稻瘟病基因鉴定,加快抗性育种进程提供了理论依据。 展开更多
关键词 水稻(Oryza SATIVA L.) 稻瘟病 抗性基因 基因定位 抗性鉴定
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Research Advances of Root Rot in Medicago sativa L. 预览
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作者 Luo Yinghua Jin Chenghao +1 位作者 Xu Meihua Liu Xiangping 《Plant Diseases and Pests(植物病虫害研究:英文版)》 CAS 2019年第1期15-17,27共4页
As the most famous perennial leguminous forage in the world,Medicago sativa L.ranks the first in both yield and nutritional value,and can be used for many years once planted in field production.However,root rot has be... As the most famous perennial leguminous forage in the world,Medicago sativa L.ranks the first in both yield and nutritional value,and can be used for many years once planted in field production.However,root rot has become a very important reason for yield decrease and plant decline of alfalfa due to long service life.According to the research progress at home and abroad,the main pathogen distribution,harms and control methods of root rot in M.sativa at home and abroad were systematically expounded,in order to provide a theoretical basis for further research of the disease. 展开更多
关键词 MEDICAGO SATIVA L. ROOT ROT FUSARIUM PATHOGEN
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Enclosed stigma contributes to higher spikelet fertility for rice(Oryza sativa L.) subjected to heat stress 预览
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作者 Chao Wu Kehui Cui +4 位作者 Qiuqian Hu Wencheng Wang Lixiao Nie Jianliang Huang Shaobing Peng 《作物学报:英文版》 CAS CSCD 2019年第3期335-349,共15页
With global warming, rice plants may be subjected to heat stress more regularly during the heatsensitive flowering stage, causing spikelet sterility and grain yield loss.Stigma exsertion is considered to increase poll... With global warming, rice plants may be subjected to heat stress more regularly during the heatsensitive flowering stage, causing spikelet sterility and grain yield loss.Stigma exsertion is considered to increase pollen reception and promote female reproductive success.The aim of this study was to investigate the role of stigma exsertion on spikelet fertility at high temperatures.Five rice cultivars(Liangyoupeijiu, Shanyou 63, Huanghuazhan, Nagina 22, and IR64) with differing degrees of stigma exsertion were cultivated and exposed to high temperature at anthesis.Heat-tolerant cultivars did not always show a high percentage of spikelets with exserted stigmas, and vice versa.Irrespective of the presence of more pollen grains on exserted stigmas, spikelets with exserted stigmas did not show greater spikelet fertility than spikelets with fewer exserted stigmas or hidden stigmas under heat stress.GA3 application augmented the percentage of spikelets with exserted stigmas;however, it did not increase spikelet fertility under heat stress.Spikelet fertility of whole panicles was negatively correlated with the percentage of spikelets with exserted stigmas, but positively with that with hidden stigmas.Viability of the hidden stigmas was less reduced than that of exserted stigmas under heat stress, suggesting that hidden stigmas have an advantage in maintaining viability.Heat stress delayed anther dehiscence and reduced the viabilities of both exserted stigmas and pollens, thereby causing low spikelet fertility.Together, these results suggest that high spikelet fertility does not depend on stigma exsertion and that enclosed stigma generally contributes to higher spikelet fertility and heat tolerance under high-temperature conditions during flowering in rice. 展开更多
关键词 Heat stress RICE (Oryza sativa L.) SPIKELET FERTILITY STIGMA exsertion STIGMA and POLLEN VIABILITY
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Genome-wide dissection of segregation distortion using multiple inter-subspecific crosses in rice
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作者 Guangwei Li Jiye Jin +5 位作者 Yan Zhou Xufeng Bai Donghai Mao Cong Tan Gongwei Wang Yidan Ouyang 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第4期507-516,共10页
Mendelian inheritance can ensure equal segregation of alleles from parents to offspring,which provides fundamental basis for genetics and molecular biology.Segregation distortion(SD)leads to preferential transmission ... Mendelian inheritance can ensure equal segregation of alleles from parents to offspring,which provides fundamental basis for genetics and molecular biology.Segregation distortion(SD)leads to preferential transmission of certain alleles from generation to generation.Such violation of Mendelian genetic principle is often accompanied by reproductive isolation and eventually speciation.Although SD is observed in a wide range of species from plants to animals,genome-wide dissection of such biased transmission of gametes is rare.Using nine inter-subspecific rice crosses,a genome-wide screen for SD loci is performed,which reveals 61 single-locus quantitative trait loci and 194 digenic interactions showing distorted transmission ratio,among which 24 new SD loci are identified.Biased transmission of alleles is observed in all nine crosses,suggesting that SD exists extensively in rice populations.72.13% distorted regions are repeatedly detected in multiple populations,and the most prevalent SD hotspot that observed in eight populations is mapped to chromosome 3.Xian alleles are transmitted at higher frequencies than geng alleles in inter-subspecific crosses,which change the genetic composition of the rice populations.Epistatic interaction contributes significantly to the deviation of Mendelian segregation at the whole-genome level in rice,which is distinct from that in animals.These results provide an extensive archive for investigating the genetic basis of SD in rice,which have significant implications in understanding the reproductive isolation and formation of inter-subspecific barriers during the evolution. 展开更多
关键词 Oryza sativa xian/indica geng/japonica SEGREGATION distortion REPRODUCTIVE isolation ALLELE frequency
Cannabis,cannabinoid receptors,and endocannabinoid system:yesterday,today,and tomorrow
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作者 Jie Wu 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第3期297-299,共3页
Cannabis sativa,is also popularly known as marijuana,has been cultivated and used for recreational and medicinal purposes for many centuries.The main psychoactive content in cannabis is Δ^9-tetrahydrocannabinol(THC).... Cannabis sativa,is also popularly known as marijuana,has been cultivated and used for recreational and medicinal purposes for many centuries.The main psychoactive content in cannabis is Δ^9-tetrahydrocannabinol(THC).In addition to plant cannabis sativa,there are two classes of cannabinoids—the synthetic cannabi-noids(e.g.,WIN55212–2)and the endogenous cannabinoids(eCB),anandamide(ANA)and 2-arachidonoylglycerol(2-AG). 展开更多
关键词 CANNABIS SATIVA popularly KNOWN MEDICINAL purposes
OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice 预览
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作者 Yahui Liu Yu Zhu +4 位作者 Xuding Xu Fan Sun Jingshui Yang Liming Cao Xiaojin Luo 《作物学报:英文版》 CAS CSCD 2019年第5期707-714,共8页
Rice(Oryza sativa L.)is a major food crop worldwide.Plant height and yield are important agronomic traits of rice.Several genes regulating plant height and/or yield have been cloned.However,the molecular mechanisms co... Rice(Oryza sativa L.)is a major food crop worldwide.Plant height and yield are important agronomic traits of rice.Several genes regulating plant height and/or yield have been cloned.However,the molecular mechanisms coordinating plant height and yield are not fully characterized.Here,we report a novel gene,OstMAPKKK5 that encodes a truncated variant of a mitogen-activated protein kinase kinase kinase 5(OsMAPKKK5)lacking an intact kinase domain.Transgenic plants overexpressing OstMAPKKK5 in indica cultivar 9311 showed increased plant height,grain length,grain width,1000-grain weight,grain number per main panicle,and yield.Real-time quantitative PCR showed that OstMAPKKK5 was widely expressed in various tissues and developmental stages.The increased plant height and yield were attributed to enlarged cell size.Overexpression of OstMAPKKK5 led to higher contents of various forms of endogenous gibberellin(GA),especially the most common active forms,GA1,GA3,GA4.We concluded that OstMAPKKK5 positively regulates plant height and yield in rice by affecting cell size,and that its underlying mechanism is based on increased endogenous GA content. 展开更多
关键词 OstMAPKKK5 Plant height and yield Cell size GIBBERELLIN Oryza sativa
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Analysis of Protein Characteristics of Selenite Transporter OsPT2 in Rice 预览
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作者 Biaojin ZHANG Qinglong CHEN +4 位作者 Tingcan DAI Yaomin ZHOU Yihua WEI Xiangxi ZHANG Linguang LUO 《农业生物技术:英文版》 CAS 2019年第1期13-16,共4页
OsPT2,which is a selenite transporter in rice,belongs to rice phosphate transporter family OsPT.In this study,the amino acid sequence information of OsPT family members was collected using NCBI database.Bioinformatics... OsPT2,which is a selenite transporter in rice,belongs to rice phosphate transporter family OsPT.In this study,the amino acid sequence information of OsPT family members was collected using NCBI database.Bioinformatics method was employed to analyze physical and chemical properties and protein structure of OsPT2 and its homologous relationship with other members of the family.The results indicated that OsPT2 was a stable alkaline hydrophobic protein embedded in the cell membrane.The protein contained 11 transmembrane domains,and consisted of 45.08%of alpha helix,16.48%of extended strand,10.80%of beta turn and 27.65%of random coil,forming a barrel-like three-dimensional structure.Members in OsPT family had high homology and could be divided into 5 subgroups.OsPT2 had closer relationship with OsPT1 and OsPT3.This study could provide a basis for further revealing of molecular mechanism of selenite transport in rice. 展开更多
关键词 Rice(Oryza SATIVA L.) SELENITE OsPT2 Protein CHARACTERISTICS BIOINFORMATICS
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Therapeutic Trials of Nigella sativa 预览
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作者 Aysenur Koc Nazan Guven +4 位作者 S1d1ka Buyukhelvac1gil Ozturk1 Burcak Deniz Dedeoglu Hale Feyza Buyukhelvacigil Ramazan Buyukhelvac1gil Beril Koparal 《药剂与药理学:英文版》 2019年第11期564-577,共14页
Nigella sativa (NS)(Ranunculaceae family) is generally utilized as a therapeutic plant all over the world. The seeds of the plant have a long history of use in different frameworks of medicines and food. N. sativa see... Nigella sativa (NS)(Ranunculaceae family) is generally utilized as a therapeutic plant all over the world. The seeds of the plant have a long history of use in different frameworks of medicines and food. N. sativa seeds reveal a broad spectrum of pharmacological activities including immunopotentiation and antihistaminic, antiseptic, antiallergic, anti-hypertensive, anti-inflammatory, and antimicrobial activities. It is known as a source of thymoquinone (TQ), thymohydroquinone, dithymoquinone, p-cymene, carvacrol, 4-terpineol, t-anethol, sesquiterpene longifolene, nigellicimine and nigellicimine-N-oxide,α-pinene and thymol etc. Additionally, this is uncovered that the majority of therapeutic properties of this plant are due to the presence of thymoquinone which is a major bioactive component of the essential oil. The incalculable medicinal properties and therapeutic uses of N. sativa prove its importance as a valuable medicinal plant. The aim of this review is to summarize some important pharmacological studies and phytochemical investigations on N. sativa and isolated principles which can be investigated further to get novel molecules in the search of novel herbal drugs. 展开更多
关键词 Nigella SATIVA MEDICINAL PLANT phytoconstituent PHARMACOLOGICAL ACTIVITY
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Mapping and genetic validation of a grain size QTL qGS7.1 in rice(Oryza sativa L.) 预览
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作者 XUE Pao ZHANG Ying-xin +7 位作者 LOU Xiang-yang ZHU Ai-ke CHEN Yu-yu SUN Bin YU Ping CHENG Shi-hua CAO Li-yong ZHAN Xiao-deng 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第8期1838-1850,共13页
Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight ... Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight in rice. QTLs were mapped using a BC4F4 population including 192 backcross inbred lines(BILs) derived from a backcross between Xiaolijing(XLJ) and recombinant inbred lines(RILs). The mapping population was planted in both Lingshui(Hainan, 2015) and Fuyang(Zhejiang, 2016), with the short-and long-day conditions, respectively. A total of 10 QTLs for grain length, four for grain width, four for the ratio of grain length to width, and 11 for grain weight were detected in at least one environment and were distributed across 11 chromosomes. The phenotypic variance explained ranged from 6.76–25.68%, 14.30–34.03%, 5.28–26.50%, and 3.01–22.87% for grain length, grain width, the ratio of grain length to width, and thousand grain weight, respectively. Using the sequential residual heterozygotes(SeqRHs) method, qGS7.1, a QTL for grain size and weight, was mapped in a 3.2-Mb interval on chromosome 7. No QTLs about grain size and weight were reported in previous studies in this region, providing a good candidate for functional analysis and breeding utilization. 展开更多
关键词 RICE (Oryza sativa L.) quantitative TRAIT LOCI GRAIN size GRAIN weight residual HETEROZYGOTE
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Effect of various crop rotations on rice yield and nitrogen use efficiency in paddy–upland systems in southeastern China 预览
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作者 Song Chen Shaowen Liu +7 位作者 Xi Zheng Min Yin Guang Chu Chunmei Xu Jinxiang Yan Liping Chen Danying Wang Xiufu Zhang 《作物学报:英文版》 CAS CSCD 2018年第6期576-588,共13页
To evaluate the effects of various rotation systems on rice grain yield and N use efficiency,a paddy–upland cropping experiment(2013–2016)was conducted in southeastern China.The experiment was designed using six dif... To evaluate the effects of various rotation systems on rice grain yield and N use efficiency,a paddy–upland cropping experiment(2013–2016)was conducted in southeastern China.The experiment was designed using six different rice––winter crop rotations:rice–fallow(RF),rice–wheat(RW),rice–potato with rice straw mulch(RP),rice–green manure(Chinese milk vetch;RC–G),rice–oilseed rape(RO),and rice–green manure crop(oilseed rape with fresh straw incorporated into soil at flowering;RO–G)and three N rates,N0(0 kg N ha^-1),N1(142.5 kg N ha^-1),and N2(202.5 kg N ha^-1).Average rice yields in the RF(5.93 t ha^-1)rotation were significantly lower than those in the rotations with winter crops(7.20–7.48 t ha^-1)under the N0 treatment,suggesting that incorporation of straw might be more effective for increasing soil N than winter fallow.The rice yield differences among the rotations varied by year with the N input.In general,the grain yields in the RP and RO–G rotations–were respectively 11.6–28.5%and 14.80–37.19%higher than those in the RF in plots with N applied.Increasing the N rate may have tended to minimize the average yield gap between the RF and the other rotations;the yield gaps were 18.55%,4.14%,and 0.23%in N0,N1,and N2,respectively.However,the N recovery efficiency in the RF was significantly lower than that in other rotations,except for 2015 under both N1 and N2 rates,a finding that implies a large amount of chemical N loss.No significant differences in nitrogen agronomic efficiency(NAE)and physiological efficiency(NPE)were found between the rotations with legume(RC–G)and non–legume(RO and RW)winter crops,a result that may be due partly to straw incorporation.For this reason,we concluded that the return of straw could reduce differences in N use efficiency between rotations with and without legume crops.The degree of synchrony between the crop N demand and the N supply was evaluated by comparison of nitrogen balance degree(NBD)values.The NBD values in the RP and RW were significant 展开更多
关键词 Rice(Oryza SATIVA L.) Paddy–upland ROTATION Nitrogen use efficiency Winter CROPS
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水杨酸对铬胁迫下紫花苜蓿幼苗生理特性的影响
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作者 杨建霞 刘肖 赵贵霞 《林业科技通讯》 2018年第9期26-29,共4页
以"甘农3号"紫花苜蓿(Medicago sativa L.)为材料,采用盆栽试验,研究外源SA对Cr6+胁迫下紫花苜蓿幼苗叶片中抗氧化酶SOD、POD及CAT的活性和渗透调节物质脯氨酸、丙二醛及叶绿素含量等逆境生理指标的影响。结果表明:1.5mmol/L和1.0... 以"甘农3号"紫花苜蓿(Medicago sativa L.)为材料,采用盆栽试验,研究外源SA对Cr6+胁迫下紫花苜蓿幼苗叶片中抗氧化酶SOD、POD及CAT的活性和渗透调节物质脯氨酸、丙二醛及叶绿素含量等逆境生理指标的影响。结果表明:1.5mmol/L和1.0mmol/L的Cr(6+)组合处理能显著提高幼苗中叶绿素的含量和SOD、POD及CAT的活性,而降低丙二醛和脯氨酸的含量。暗示一定浓度的外源SA可能通过激活抗氧化酶SOD、POD及CAT的活性,加快活性氧物质的清除速度,缓解重金属胁迫对幼苗叶片造成的氧化损伤,进而提高紫花苜蓿的抗逆性。 展开更多
关键词 水杨酸 铬胁迫 紫花苜蓿 MEDICAGO SATIVA L. 逆境生理指标
一个新的水稻斑马叶突变体的发现及初步研究 预览
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作者 尹建英 谭军 郭国强 《农业科技通讯》 2018年第7期97-101,共5页
从恢复系育种材料[R128//(R318/R1025)F1]F6中获得一个新的斑马叶突变体zebra1349,该突变体秧苗期如果不移栽,与野生型一样表现绿色,移栽后5 d新抽出的叶片包括叶鞘会呈现出与叶脉垂直的黄绿相间的条纹,移栽后30 d抽出的叶片又表现出... 从恢复系育种材料[R128//(R318/R1025)F1]F6中获得一个新的斑马叶突变体zebra1349,该突变体秧苗期如果不移栽,与野生型一样表现绿色,移栽后5 d新抽出的叶片包括叶鞘会呈现出与叶脉垂直的黄绿相间的条纹,移栽后30 d抽出的叶片又表现出正常绿色,成熟期主要农艺性状与野生型无明显差异。不同叶龄移栽试验表明,叶龄对zebra1349斑马叶性状的表达没有影响,不同播期移栽试验表明,温度对zebra1349斑马叶性状的表达有一定影响。对zebra1349与正常叶色品种杂交F1、F2代的遗传分析表明,该性状受1对隐性核基因调控。 展开更多
关键词 水稻(Oryza SATIVA L.) 斑马叶突变体 叶色转变特性 遗传分析
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