期刊文献+
共找到3,820篇文章
< 1 2 191 >
每页显示 20 50 100
细胞色素P4501B1在乳腺纤维腺瘤组织的表达及临床意义
1
作者 杨颖涛 段奇 +1 位作者 赤更 何晓燕 《中华实验外科杂志》 CAS CSCD 北大核心 2019年第7期1290-1291,共2页
目的探讨细胞色素P4501B1(CYP1B1)在乳腺纤维腺瘤中的表达及其与患者临床病理参数的作用机制.方法收集2014年3月至2016年11月郑州大学第五附属医院病理证实为乳腺纤维腺瘤患者40例(均为女性,中位年龄24岁),检测CYP1B1蛋白的表达,及40例... 目的探讨细胞色素P4501B1(CYP1B1)在乳腺纤维腺瘤中的表达及其与患者临床病理参数的作用机制.方法收集2014年3月至2016年11月郑州大学第五附属医院病理证实为乳腺纤维腺瘤患者40例(均为女性,中位年龄24岁),检测CYP1B1蛋白的表达,及40例正常组织中相关蛋白的表达,分析其与患者临床病理参数的关系.应用SPSS 19.0统计软件分析,CYP1B1的表达与乳腺纤维腺瘤的临床病理学参数之间采用x^2检验,两者相关性采用Spearman等级相关分析法.结果乳腺纤维腺瘤患者肿瘤组织中的CYP1B1蛋白表达(57.5%)明显高于正常组织(20.0%),差异有统计学意义(x^2=11.850,P<0.05),CYP1 B1蛋白表达与患者年龄大小差异有统计学意义(x^2=12.675,P<0.05),与肿瘤大小比较差异无统计学意义(x^2=0.073,P> 0.05).结论 CYP1B1蛋白与乳腺纤维腺瘤的生成、发展明显相关. 展开更多
关键词 乳腺纤维腺瘤 细胞色素P4501B1蛋白 P450
仙灵骨葆单独及与奥美拉唑联合应用的肝毒性研究 预览
2
作者 丁雁南 马红红 +4 位作者 石富国 徐亚沙 王洋 鲁艳柳 陆远富 《遵义医学院学报》 2019年第3期265-271,共7页
目的考察仙灵骨葆及其与奥美拉唑联用对小鼠的肝毒性。方法雄性C57小鼠随机分为空白对照组、仙灵骨葆低和高剂量组(308、 2 464 mg/kg)、奥美拉唑组(8.22 mg/kg)及仙灵骨葆低、高剂量联用奥美拉唑组。各组连续给药27周,其中联用组先灌... 目的考察仙灵骨葆及其与奥美拉唑联用对小鼠的肝毒性。方法雄性C57小鼠随机分为空白对照组、仙灵骨葆低和高剂量组(308、 2 464 mg/kg)、奥美拉唑组(8.22 mg/kg)及仙灵骨葆低、高剂量联用奥美拉唑组。各组连续给药27周,其中联用组先灌胃给予仙灵骨葆13周,再同时腹腔注射给予奥美拉唑14周。每周测定小鼠体重,末次给药后12 h处死小鼠,测定肝脏重量、肝系数及血清谷丙转氨酶、谷草转氨酶、碱性磷酸酶活性,观察肝组织形态学变化,RT-PCR法检测肝脏Cyp1a2、Cyp3a11、Cyp2c37等主要药物代谢P450酶基因表达水平。结果各给药组小鼠体重增长率、肝脏重量均低于空白对照组。仙灵骨葆及其联用奥美拉唑对谷丙转氨酶、谷草转氨酶、碱性磷酸酶活性影响较小,但可造成肝组织炎细胞浸润并且能显著增加小鼠肝脏Cyp1a2、Cyp2c37 mRNA的表达。结论连续给予小鼠27周仙灵骨葆引起以肝组织炎细胞浸润为特征的肝损伤,仙灵骨葆联合奥美拉唑小鼠肝损伤程度与单用仙灵骨葆相当。 展开更多
关键词 仙灵骨葆 奥美拉唑 肝毒性 细胞色素P450
在线阅读 免费下载
草地贪夜蛾解毒代谢相关基因家族的进化分析 预览
3
作者 梅洋 杨义 +2 位作者 叶昕海 肖花美 李飞 《环境昆虫学报》 CSCD 北大核心 2019年第4期727-735,共9页
近年来草地贪夜蛾Spodoptera frugiperda在世界各地猖獗为害,其寄主范围广,且已对多种化学药剂和转Bt玉米产生抗性。本文对包括草地贪夜蛾在内的14种昆虫进行了解毒代谢相关基因家族的注释,在草地贪夜蛾中发现了152个细胞色素P450(cytoc... 近年来草地贪夜蛾Spodoptera frugiperda在世界各地猖獗为害,其寄主范围广,且已对多种化学药剂和转Bt玉米产生抗性。本文对包括草地贪夜蛾在内的14种昆虫进行了解毒代谢相关基因家族的注释,在草地贪夜蛾中发现了152个细胞色素P450(cytochrome p450,P450)、92个ABC转运蛋白(ATP-binding cassette transporter,ABC transporter)、44个谷胱甘肽转移酶(glutathione s-transferase,GST)以及39个尿苷二磷酸葡萄糖醛酸转移酶(UDP-glucuronosyl-transferase,UGT)。与其他鳞翅目物种相比,草地贪夜蛾的P450和GST基因数量最多,其中152个P450基因可被归类至Mito、CYP2、CYP3和CYP4四个簇中;Mito和CYP2簇的P450基因总数与家蚕Bombyx mori相近,但CYP3和CYP4簇的P450基因明显多于家蚕,进化分析结果显示CYP3和CYP4簇的P450基因发生了明显的扩增;与家蚕P450基因的共线性分析发现草地贪夜蛾89个P450基因在其基因组上呈簇分布并发生了簇的扩增;与家蚕相比,GST和ABC转运蛋白家族基因的数量明显增多。UGT基因家族没有明显增加,但在基因进化分析中有成簇现象,表明可能存在近期的扩增事件。本研究首次从基因组水平鉴定了草地贪夜蛾解毒代谢相关基因家族,对理解草地贪夜蛾的生物学特性及抗性的形成具有重要指导意义。 展开更多
关键词 草地贪夜蛾 P450 GST UGT 扩增
在线阅读 下载PDF
寄生蜂细胞色素P450基因家族进化分析 预览
4
作者 尹传林 叶昕海 +3 位作者 陈梦瑶 梅洋 肖花美 李飞 《中国生物防治学报》 CSCD 北大核心 2019年第3期335-342,共8页
寄生蜂是重要的天敌昆虫,被广泛应用于生物防治,但在实际生产应用中面临诸多问题,如环境适应性和耐药性问题。细胞色素P450是真核生物中广泛存在的超基因家族酶系,参与农药、植物次生代谢物及环境有害物质等的代谢,同时也参与昆虫体内... 寄生蜂是重要的天敌昆虫,被广泛应用于生物防治,但在实际生产应用中面临诸多问题,如环境适应性和耐药性问题。细胞色素P450是真核生物中广泛存在的超基因家族酶系,参与农药、植物次生代谢物及环境有害物质等的代谢,同时也参与昆虫体内多种内源性化合物的合成与代谢。本文通过对植食性榕小蜂和9种寄生蜂基因组数据的生物信息学分析,共获得615个P450基因,分析表明不同寄生蜂P450基因家族的差异主要发生在CYP3和CYP4簇,而CYP2和Mito簇相对保守;相较于外寄生蜂,内寄生蜂的P450超基因家族有明显的收缩现象;此外,发现了2个在寄生蜂中1:1:1高度保守的P450基因,CYP18和CYP314,以及茧蜂特有的CYP304基因和内寄生蜂特有的CYP28基因。本研究从基因组水平系统地分析了寄生蜂P450超基因家族的进化,为寄生蜂的遗传改良和规模化饲养等提供理论基础。 展开更多
关键词 寄生蜂 P450基因 基因家族进化分析 内寄生与外寄生
在线阅读 免费下载
Role of cytochrome P450 polymorphisms and functions in development of ulcerative colitis 预览
5
作者 Alaattin Sen Holger Stark 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第23期2846-2862,共17页
Cytochromes P450 s(CYPs) are terminal enzymes in CYP dependent monooxygenases, which constitute a superfamily of enzymes catalysing the metabolism of both endogenous and exogenous substances. One of their main tasks i... Cytochromes P450 s(CYPs) are terminal enzymes in CYP dependent monooxygenases, which constitute a superfamily of enzymes catalysing the metabolism of both endogenous and exogenous substances. One of their main tasks is to facilitate the excretion of these substances and eliminate their toxicities in most phase 1 reactions. Endogenous substrates of CYPs include steroids, bile acids, eicosanoids, cholesterol, vitamin D and neurotransmitters. About 80% of currently used drugs and environmental chemicals comprise exogenous substrates for CYPs. Genetic polymorphisms of CYPs may affect the enzyme functions and have been reported to be associated with various diseases and adverse drug reactions among different populations. In this review, we discuss the role of some critical CYP isoforms(CYP1 A1, CYP2 D6, CYP2 J2, CYP2 R1,CYP3 A5, CYP3 A7, CYP4 F3, CYP24 A1, CYP26 B1 and CYP27 B1) in the pathogenesis or aetiology of ulcerative colitis concerning gene polymorphisms. In addition, their significance in metabolism concerning ulcerative colitis in patients is also discussed showing a clear underestimation in genetic studies performed so far. 展开更多
关键词 CYTOCHROME P450 PolymorPhism Function ULCERATIVE COLITIS AETIOLOGY
在线阅读 免费下载
Optimizing proton pump inhibitors in Helicobacter pylori treatment:Old and new tricks to improve effectiveness 预览
6
作者 Enzo Ierardi Giuseppe Losurdo +3 位作者 Rosa Federica La Fortezza Mariabeatrice Principi Michele Barone Alfredo Di Leo 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第34期5097-5104,共8页
The survival and replication cycle of Helicobacter pylori(H.pylori)is strictly dependant on intragastric pH,since H.pylori enters replicative phase at an almost neutral pH(6-7),while at acid pH(3-6)it turns into its c... The survival and replication cycle of Helicobacter pylori(H.pylori)is strictly dependant on intragastric pH,since H.pylori enters replicative phase at an almost neutral pH(6-7),while at acid pH(3-6)it turns into its coccoid form,which is resistant to antibiotics.On these bases,it is crucial to increase intragastric pH by proton pump inhibitors(PPIs)when an antibiotic-based eradicating therapy needs to be administered.Therefore,several tricks need to be used to optimize eradication rate of different regimens.The administration of the highest dose as possible of PPI,by doubling or increasing the number of pills/day,has shown to be able to improve therapeutic outcome and has often proposed in rescue therapies,even if specific trials have not been performed.A pre-treatment with PPI before starting antibiotics does not seem to be effective,therefore it is discouraged.However,the choice of PPI molecule could have a certain weight,since second-generation substances(esomeprazole,rabeprazole)are likely more effective than those of first generation(omeprazole,lansoprazole).A possible explanation is due to their metabolism,which has been proven to be less dependent on cytochrome P450(CYP)2C19 genetic variables.Finally,vonoprazan,a competitive inhibitor of H+/K+-ATPase present on luminal membrane of gastric parietal cells has shown the highest efficacy,due to both its highest acid inhibition power and rapid pharmacologic effect.However current data come only from Eastern Asia,therefore its strong power needs to be confirmed outside this geographic area in Western countries as well as related to the local different antibiotic resistance rates. 展开更多
关键词 HELICOBACTER PYLORI Proton pump inhibitors ERADICATION CYTOCHROME P450 Optimization
在线阅读 免费下载
A structural and data-driven approach to engineering a plant cytochrome P450 enzyme
7
作者 Dawei Li Yongshuo Ma +9 位作者 Yuan Zhou Junbo Gou Yang Zhong Lingling Zhao Lida Han Sergey Ovchinnikov Ling Ma Sanwen Huang Per Greisen Yi Shang 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第7期873-882,共10页
Functional manipulation of biosynthetic enzymes such as cytochrome P450 s(or P450 s) has attracted great interest in metabolic engineering of plant natural products.Cucurbitacins and mogrosides are plant triterpenoids... Functional manipulation of biosynthetic enzymes such as cytochrome P450 s(or P450 s) has attracted great interest in metabolic engineering of plant natural products.Cucurbitacins and mogrosides are plant triterpenoids that share the same backbone but display contrasting bioactivities.This structural and functional diversity of the two metabolites can be manipulated by engineering P450 s.However,the functional redesign of P450 s through directed evolution(DE) or structure-guided protein engineering is time consuming and challenging,often because of a lack of high-throughput screening methods and crystal structures of P450 s.In this study,we used an integrated approach combining computational protein design,evolutionary information,and experimental data-driven optimization to alter the substrate specificity of a multifunctional P450(CYP87 D20)from cucumber.After three rounds of iterative design and evaluation of 96 protein variants,CYP87 D20,which is involved in the cucurbitacin C biosynthetic pathway,was successfully transformed into a P450 mono-oxygenase that performs a single specific hydroxylation at C11 of cucurbitadienol.This integrated P450-engineering approach can be further applied to create a de novo pathway to produce mogrol,the precursor of the natural sweetener mogroside,or to alter the structural diversity of plant triterpenoids by functionally manipulating other P450 s. 展开更多
关键词 plant P450 ENGINEERING protein design ROSETTA amino acid CO-EVOLUTION CUCURBITACIN MOGROSIDE
Association between CYP2C19*2/*3Polymorphisms and Coronary Heart Disease 预览
8
作者 Ying-ying ZHANG Xin ZHOU +4 位作者 Wen-jie JI Ting LIU Jing MA Ying ZHANG Yu-ming LI 《当代医学科学(英文)》 SCIE CAS 2019年第1期44-51,共8页
This study sought to explore the relationship between cytochrome P450 2C19(CYP2C19)*2/*3 polymorphisms and the development of coronary heart disease(CHD),and to evaluate the influence of the single nucleotide polymorp... This study sought to explore the relationship between cytochrome P450 2C19(CYP2C19)*2/*3 polymorphisms and the development of coronary heart disease(CHD),and to evaluate the influence of the single nucleotide polymorphisms(SNPs)on the occurrence of adverse clinical events in CHD patients.A total of 231 consecutive patients candidate for percutaneous coronary intervention genotyped for CYP2C19*2(681G>A)and*3(636G>A)polymorphisms were enrolled.The adverse clinical events were recorded during a follow-up period of 14 months.The incidence of CHD,according to coronary angiography,was significantly higher(P=0.025)in CYP2C19*2 carriers group.Stepwise binary logistic regression analysis revealed that among factors that potentially influenced the presence of CHD(age>60 years,gender,BMI,etc.),CYP2C19*2 carriers(OR 1.94,95%CI:1.08-3.50,P=0.028)and male gender(OR 2.74,95%CI:1.58-4.76,P=0.001)were independent predictors,which were associated with the presence of CHD.The follow-up results showed that the incidence of adverse cardiovascular events within 14 months of discharge was significantly higher in the CYP2C 19*2 carriers than in the non-carriers(21.6%vs.6.3%,P=0.019).The results of the multivariate Cox proportional hazards model showed that CYP2C19*2 loss-of-flinction was the only independent factor which predicted the coronary events during the follow-up period of 14 months(OR=3.65,95%CI:1.09-12.25,P=0.036).The adverse impact of CYP2C19*2 polymorphisms was found not only in the risk of the presence of CHD,but also in the adverse cardiovascular events in CHD patients during the follow-up period of 14 months.However the same influence was not found in CYP2C19*3 mutation in Chinese Han population. 展开更多
关键词 CYTOCHROME P450 2C19 POLYMORPHISMS coronary heart disease CLOPIDOGREL
在线阅读 下载PDF
Insecticide toxicity associated with detoxification enzymes and genes related to transcription of cuticular melanization among color morphs of Asian citrus psyllid
9
作者 Xue Dong Chen Torrence A. Gill +3 位作者 Chi D.Nguyen Nabil Killiny Kirsten S.Pelz-Stelinski Lukasz L.Stelinski 《昆虫科学:英文版》 SCIE CAS CSCD 2019年第5期843-852,共10页
The Asian citrus psyllid (Diaphorina citri Kuwayama) is known to exhibit abdominal color polymorphisms. In the current study, susceptibility to four insecticides was compared among orange/yellow, blue/green and gray/b... The Asian citrus psyllid (Diaphorina citri Kuwayama) is known to exhibit abdominal color polymorphisms. In the current study, susceptibility to four insecticides was compared among orange/yellow, blue/green and gray/brown color morphs of field collected D. citri. The LD50 values and 95% fiducial limits were quantified for each insecticide and color morph combination and ranged between 0.10 ng/μL (0.06-0.10) and 6.16 ng//μL (3.30-12.50). Second, we measured the detoxification enzyme activity levels of orange/yellow, blue/green and gray/brown color morphs for cytochrome P450, glutathione "S-transferase, and general esterase. The mean P450 activity (equivalent units) was significantly lower in gray/brown (0.152 ± 0.006) and blue/green morphs (0.149 ± 0.005) than in the orange/yellow morphs (0.179 ± 0.008). GST activity (/imol/min/mg protein) was significantly lower in the orange/yellow morph (299.70 ± 1.24) than gray/brown (350.86 ± 1.19) and blue/green (412.25 ±1.37) morphs. The mean EST activity (/xmol/min/mg protein) was significantly higher in blue/green (416.72±5.12) and gray/brown morphs (362.19 ± 4.69) than in the orange/yellow morphs (282.56 ± 2.93). Additionally, we analyzed the relative expression of assortment genes involved in cuticular melanization and basal immunity. The transcripts of Dopa Decarboxylase and Tyrosine Hydroxylase were expressed higher in blue/green and gray/brown than orange/yellow morphs. The transcription results paralleled the susceptibility of D.citri to organophosphate, neonicotinoid and pyrethroid insecticides. GST and EST activity may also be correlated with low levels of insecticide susceptibility. Cuticular melanization could be a factor for the development of resistance to insecticides among different color morphs. 展开更多
关键词 cuticular MELANIZATION CYTOCHROME P450 ESTERASE gene expression GLUTATHIONE .S-transferase INSECTICIDE susceptibility
A hepatoprotection study of Radix Bupleuri on acetaminophen-induced liver injury based on CYP450 inhibition
10
作者 WANG Yu-Xin DU Yi +7 位作者 LIU Xia-Fei YANG Fang-Xiu WU Xiao TAN Li LU Yi-Hong ZHANG Jing-Wei ZHOU Fang WANG Guang-Ji 《中国天然药物:英文版》 SCIE CAS CSCD 2019年第7期517-524,共8页
We investigated the potential hepatoprotective effect of Radix Bupleuri(RB) by inducing acute liver injury(ALI) in an animal model using acetaminophen(APAP) after pretreatment with RB aqueous extract for three consecu... We investigated the potential hepatoprotective effect of Radix Bupleuri(RB) by inducing acute liver injury(ALI) in an animal model using acetaminophen(APAP) after pretreatment with RB aqueous extract for three consecutive days. Compared to those of the APAP group, the biochemical and histological results of the RB pretreatment group showed lower serum aspartate transaminase(AST) and alanine transaminase(ALT) levels as well as less liver damage. Pharmacokinetic study of the toxicity related marker acetaminophen-cysteine(APC) revealed a lower exposure level in rats, suggesting that RB alleviated APAP-induced liver damage by preventing glutathione(GSH) depletion. The results of cocktail approach showed significant inhibition of CYP2 E1 and CYP3 A activity. Further investigation revealed the increasing of CYP2 E1 and CYP3 A protein was significantly inhibited in pretreatment group,while no obvious effect on gene expression was found. Therefore, this study clearly demonstrates that RB exhibited significant protective action against APAP-induced acute live injury via pretreatment, and which is partly through inhibiting the increase of activity and translation of cytochrome P450 enzymes, rather than gene transcription. 展开更多
关键词 HEPATOPROTECTION RADIX Bupleuri ACETAMINOPHEN Acute liver injury CYTOCHROME P450 enzymes
Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations 预览
11
作者 Lanqing Liu Hong Liu +4 位作者 Wei Zhang Mingdong Yao Bingzhi Li Duo Liu Yingjin Yuan 《工程(英文)》 2019年第2期287-295,共9页
Engineering the biosynthesis of plant-derived natural products in microbes presents several challenges, especially when the expression and activation of the plant cytochrome P450 enzyme is required. By recruiting two ... Engineering the biosynthesis of plant-derived natural products in microbes presents several challenges, especially when the expression and activation of the plant cytochrome P450 enzyme is required. By recruiting two enzymes—HpaB and HpaC—from several bacteria, we constructed functional 4- hydroxyphenylacetate 3-hydroxylase (4HPA3H) in Saccharomyces cerevisiae to take on a role similar to that of the plant-derived cytochrome P450 enzyme and produce caffeic acid. Along with a common tyrosine ammonia lyase (TAL), the different combinations of HpaB and HpaC presented varied capabilities in producing the target product, caffeic acid, from the substrate, L-tyrosine. The highest production of caffeic acid was obtained with the enzyme combination of HpaB from Pseudomonas aeruginosa and HpaC from Salmonella enterica, which yielded up to (289.4 ± 4.6) mg-L1 in shake-flask cultivation. The compatibility of heterologous enzymes within a yeast chassis was effectively improved, as the caffeic acid production was increased by 40 times from the initial yield. Six key amino acid residues around the flavin adenine dinucleotide (FAD) binding domain in HpaB from Pseudomonas aeruginosa were differentiate from those other HpaBs, and might play critical roles in affecting enzyme activity. We have thus established an effective approach to construct a highly efficient yeast system to synthesize non-native hydroxylated phenylpropanoids. 展开更多
关键词 SACCHAROMYCES CEREVISIAE Caffeic acid HETEROLOGOUS enzyme CYTOCHROME P450 Synthetic biology
在线阅读 免费下载
Tobacco smoking and its drug interactions with comedications involving CYP and UGT enzymes and nicotine 预览
12
作者 Naina Mohamed Pakkir Maideen 《世界药理学杂志》 2019年第2期14-25,共12页
Tobacco smoking is a global public health threat causing several illnesses including cardiovascular disease(Myocardial infarction),cerebrovascular disease(Stroke),peripheral vascular disease(Claudication),chronic obst... Tobacco smoking is a global public health threat causing several illnesses including cardiovascular disease(Myocardial infarction),cerebrovascular disease(Stroke),peripheral vascular disease(Claudication),chronic obstructive pulmonary disease,asthma,reduced female infertility,sexual dysfunction in men,different types of cancer and many other diseases.It has been estimated in 2015 that approximately 1.3 billion people smoke,around the globe.Use of medications among smokers is more common,nowadays.This review is aimed to identify the medications affected by smoking,involving Cytochrome P450(CYP)and uridine diphosphate-glucuronosyltransferases(UGTs)enzymes and Nicotine.Polycyclic aromatic hydrocarbons(PAHs)of tobacco smoke have been associated with the induction of CYP enzymes such as CYP1A1,CYP1A2 and possibly CYP2E1 and UGT enzymes.The drugs metabolized by CYP1A1,CYP1A2,CYP2E1 and UGT enzymes might be affected by tobacco smoking and the smokers taking medications metabolized by those enzymes,may need higher doses due to decreased plasma concentrations through enhanced induction by PAHs of tobacco smoke.The prescribers and the pharmacists are required to be aware of medications affected by tobacco smoking to prevent the toxicityassociated complications during smoking cessation. 展开更多
关键词 Drug Interactions Tobacco smoking CYTOCHROME P450 ENZYMES URIDINE diphosphate-glucuronosyltransferases ENZYMES NICOTINE
在线阅读 免费下载
The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides
13
作者 Bo Hu Shu-Heng Zhang +4 位作者 Miao-Miao Ren Xiang-Rui Tian Qi Wei David Kibe Mburu Jian-Ya Su 《昆虫科学:英文版》 SCIE CAS CSCD 2019年第2期199-216,共18页
Cytochrome P450 and UDP-glucosyltransferase (UGT) as phase I and phase II metabolism enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics. Insects can in crease the expression of det... Cytochrome P450 and UDP-glucosyltransferase (UGT) as phase I and phase II metabolism enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics. Insects can in crease the expression of detoxificatio n enzymes to cope with the stress from xenobiotics including insecticides. However, the molecular mechanisms for insecticide detoxification in Spodoptera exigua remain elusive, and the genes conferring insecticide metabolisms in this species are less well reported. In this study, 68 P450 and 32 UGT genes were identified. Phylogenetic analysis showed gene expansions in CYP3 and CYP4 clans of P450 genes and UGT33 family of this pest. P450 and UGT genes exhibited specific tissue expression patterns. Insecticide treatments in fat body cells of S. exigua revealed that the expression levels of P450 and UGT genes were significantly influenced by challenges of abamectin, lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb. Multiple genes for detoxification were affected in expression levels after insecticide exposures. The results demonstrated that lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb induced similar responses in the expression of P450 and UGT genes in fat body cells;eight P450 genes and four UGT genes were co-up-regulated significantly, and no or only a few CYP/UGT genes were down-regulated significantly by these four insecticides. However, abamectin triggered a distinct response for P450 and UGT gene expression;more P450 and UGT genes were down-regulated by abamectin than by the other four compounds. In con elusion, P450 and UGT genes from S. exigua were identified, and different responses to abamectin suggest a different mechanism for insecticide detoxification. 展开更多
关键词 ABAMECTIN cytochrome P450 MONOOXYGENASES gene EXPRESSION insecticide Spodoptera EXIGUA UDP-glycosyltransferase
参麦注射液药代动力学研究进展 预览
14
作者 杨林 张升 +3 位作者 徐晓珍 顾利强 由振强 辛艳飞 《海峡药学》 2018年第11期27-30,共4页
参麦注射液是由红参和麦冬经提炼精制而成的中药复方注射剂,临床上主要用于病毒性心肌炎、冠心病等疾病的治疗。人参皂苷和麦冬皂苷是其主要活性成分,近年来围绕参麦注射液中皂苷类成分在体内的药代动力学研究不断丰富。本文就近年来参... 参麦注射液是由红参和麦冬经提炼精制而成的中药复方注射剂,临床上主要用于病毒性心肌炎、冠心病等疾病的治疗。人参皂苷和麦冬皂苷是其主要活性成分,近年来围绕参麦注射液中皂苷类成分在体内的药代动力学研究不断丰富。本文就近年来参麦注射液中皂苷类化学成分的药代动力学研究进展进行综述,为相关研究提供参考。 展开更多
关键词 参麦注射液 人参皂苷 药代动力学 P450
在线阅读 下载PDF
小麦细胞色素P450基因克隆及其序列研究 预览
15
作者 高璇 《生物化工》 2018年第5期37-39,共3页
细胞色素P450广泛存在于生物界中,它是一种含亚铁血红素单加氧酶,对多种生物的防御反应起到重要的作用。本文研究使用RACE方式,从普通小麦中克隆出新的P450基因,并将其命名为TAP450。这种新基因的基因组序列和普通的P450不同,全长2... 细胞色素P450广泛存在于生物界中,它是一种含亚铁血红素单加氧酶,对多种生物的防御反应起到重要的作用。本文研究使用RACE方式,从普通小麦中克隆出新的P450基因,并将其命名为TAP450。这种新基因的基因组序列和普通的P450不同,全长2643bp,有2个外显子和1个内含子蛋白序列和已知的P450蛋白的差异性较大。蛋白分子量是57.092kD。这种新的P450基因在小麦的叶片、茎和种子中都存在,胁迫处理下,这种基因的表达量上调。 展开更多
关键词 小麦 细胞色素 P450 基因克隆
在线阅读 下载PDF
山茱萸对老龄小鼠肝微粒体细胞色素P450部分亚型的影响 预览
16
作者 吕静 曲雷鸣 龚伟 《西部中医药》 2018年第1期9-12,共4页
目的:探讨山茱萸对老龄小鼠肝微粒体细胞色素P450部分亚型的影响。方法:对12月龄小鼠给予含山茱萸不同剂量的药物鼠粮,连续给药3个月后,以6月龄小鼠为成年对照组,采用荧光定量PCR法检测小鼠肝脏组织CYP1A1、CYP2A4、CYP2A5、CYP3A41等P... 目的:探讨山茱萸对老龄小鼠肝微粒体细胞色素P450部分亚型的影响。方法:对12月龄小鼠给予含山茱萸不同剂量的药物鼠粮,连续给药3个月后,以6月龄小鼠为成年对照组,采用荧光定量PCR法检测小鼠肝脏组织CYP1A1、CYP2A4、CYP2A5、CYP3A41等P450亚型基因mRNA的表达。结果:模型组小鼠肝脏组织CYP1A1、CYP2A4、CYP2A5、CYP3A41等基因mRNA的表达量显著低于正常组小鼠;不同剂量山茱萸给药组肝脏CYP1A1、CYP2A4、CYP2A5、CYP3A41等基因mRNA的表达量均显著高于模型组,且山茱萸高剂量组作用优于低剂量组。结论:衰老可导致细胞色素P450亚型基因表达减少,山茱萸对老龄小鼠肝微粒体CYP1A1、CYP2A4、CYP2A5、CYP3A41等基因具有诱导作用。 展开更多
关键词 肝微粒体细胞色素 P450 山茱萸
在线阅读 下载PDF
细胞色素P450异构体CYP3A4*1G基因多态性对老年直肠癌手术患者芬太尼用药量及术后镇痛效果的影响 预览
17
作者 李礼 曾剑锋 +2 位作者 侯本超 刘伟成 占燕平 《实用医学杂志》 北大核心 2018年第22期3705-3708,共4页
目的探讨细胞色素P450异构体CYP3A4*1G基因多态性对老年直肠癌手术患者芬太尼用药量及术后镇痛效果的影响。方法选取2016年2月至2018年5月于我院行直肠癌根治术的老年患者共173例,通过术前外周血PCR和测序评估CYP3A4*1G的基因分型,对比C... 目的探讨细胞色素P450异构体CYP3A4*1G基因多态性对老年直肠癌手术患者芬太尼用药量及术后镇痛效果的影响。方法选取2016年2月至2018年5月于我院行直肠癌根治术的老年患者共173例,通过术前外周血PCR和测序评估CYP3A4*1G的基因分型,对比CYP3A4*1G突变(突变组)和野生型(野生组)患者其术中芬太尼用药量,采用视觉模拟评分(VAS)用于在术后不同时间点评估患者的疼痛程度,对比患者自控镇痛(PCA)的初次镇痛时间和术后24h芬太尼使用量。结果在所有173例患者中,CYP3A4*1G变异等位基因的频率为0.191,其中突变组共61例,野生组共112例。其中突变组术中使用的平均芬太尼药物总量显著低于野生组,差异具有统计学意义(t=7.662,P<0.001),突变组患者术后初次追加镇痛时间显著高于野生组(t=7.279,P<0.001),且突变组术后24h芬太尼用量显著低于野生组(t=13.847,P<0.001),突变组在术后2、4和8h的疼痛VAS评分均显著低于野生组(P<0.05)。结论CYP3A4*1G基因多态性在老年直肠癌手术麻醉中与芬太尼的药代动力学密切相关,CYP3A4*1G遗传多态性可能是导致患者对芬太尼镇痛反应的个体差异的重要因素之一。 展开更多
关键词 P450 CYP3A4 芬太尼 直肠癌 镇痛
在线阅读 免费下载
CYP3A4 inducer and inhibitor strongly affect the pharmacokinetics of triptolide and its derivative in rats
18
作者 Ye XU Yi-fan ZHANG +1 位作者 Xiao-yan CHEN Da-fang ZHONG 《中国药理学报:英文版》 SCIE CAS CSCD 2018年第8期1386-1392,共7页
Triptolide 是 Tripterygium wilfordii 钩 F 的最活跃的成分,它被用来对待风湿性关节炎。(5R )-5-Hydroxytriptolide 是有减少的毒性的 triptolide 的 hydroxylation 衍生物。调查有酶 inducers 或禁止者的二混合物和药药相互作用的... Triptolide 是 Tripterygium wilfordii 钩 F 的最活跃的成分,它被用来对待风湿性关节炎。(5R )-5-Hydroxytriptolide 是有减少的毒性的 triptolide 的 hydroxylation 衍生物。调查有酶 inducers 或禁止者的二混合物和药药相互作用的新陈代谢的酶,一系列在里面 vitro 并且在 vivo,实验被进行。在用 recombinant 人细胞色素 P450 的 vitro 研究,酶证明细胞色素 P450 3A4 (CYP3A4 ) 在 triptolide 的新陈代谢是占优势的并且(5R ) 为 94.2% 新陈代谢和 64.2% 的 -5-hydroxytriptolide, 财务分别地。Pharmacokinetics 研究在跟随 triptolide 的管理的男 SD 老鼠被进行或(5R )-5-hydroxytriptolide (0.4 mg/kg, po ) 。血浆暴露到 triptolide 并且( 5R )当与 CYP3a 禁止者 ritonavir 共同管理时,在老鼠的 -5-hydroxytriptolide 显著地被增加( 30 mg/kg , po )与 AUC 的价值,<sub>0-</sub>(在从零外推到无穷的时间的血浆集中时间曲线下面的区域)分别地到 6.84 和 1.83 次正在被增加。什么时候有 CYP3a inducer dexamethasone 的 pretreated (50 mg 潭獮牴瑡摥琠慨 ??? 楳湧晩捩湡汴 ? 敲畤散 ? 桴 ? 敳牣瑥潩 ? 景朠獡牴捩愠楣 ? 楶? 潤湷爭来汵瑡湩 ? 桴 ? 敬敶 ? 景朠獡牴湩 ? 桷捩 ? 慷 ? 敲灳湯楳汢? 潦 ? 桴 ? 牰瑯 ' 虡 N 敶攠晦捥 ?景传呃愠慧湩瑳?? 展开更多
关键词 衍生物 老鼠 CYP3A 风湿性关节炎 新陈代谢 相互作用 细胞色素 P450
Genetic variation of CYP2R1 gene on allergic rhinitis in hainan province
19
作者 Sai-Ming Chen Xiao-Liu Zhou +4 位作者 Zhi-Qun Li Zhi-Lu Li Si-Bei Pang Hong-Zhen Shu Li-Min Zhou 《海南医科大学学报(英文版)》 2018年第21期9-11,共3页
Objective: To explore the effect of gene polymorphism locus rs12794714 on allergic rhinitis in hainan province, providing reference for genetic diagnosis and prevention. Methods:From March 2017 to December 2017, 94 lo... Objective: To explore the effect of gene polymorphism locus rs12794714 on allergic rhinitis in hainan province, providing reference for genetic diagnosis and prevention. Methods:From March 2017 to December 2017, 94 local patients with Allergic rhinitis in hainan and 113 healthy people who were admitted to the first affiliated hospital of hainan medical college during the physical examination in the physical examination department of hainan medical college were selected. Statistical software SPSS 25.0 was used to conduct inter-group differentiation analysis on genotypes and alleles, and P<0.05 was considered to be statistically different. Results: all the polymorphisms of rs12794714 in hainan population could be detected with the distribution frequency of aa-ag and GG in the Allergic rhinitis group of 20.2%, 46.8%and 33%, and in the control group of 18.6%, 38% and 43.4%. Codominant model, dominant model, recessive model and superdominant model validation were not statistically significant. The distribution frequencies of alleles A and G were 54% and 46% in the experimental group, 63% and 37% in the control group, with no statistical difference. Conclusion: rs12794714 single nucleotide polymorphism has no significant effect on the pathogenesis of Allergic rhinitis in hainan province. 展开更多
关键词 CYTOCHROME P450 family VITAMIN D signaling pathway Genetic POLYMORPHISM
Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize(Zea mays L.) 预览
20
作者 LIU Xiao-min XU Xian +4 位作者 LI Bing-hua YAO Xiao-xia ZHANG Huan-huan WANG Gui-qi HAN Yu-jun 《农业科学学报:英文版》 SCIE CAS CSCD 2018年第8期1790-1799,共10页
Postemergence application of nicosulfuron for weed control in maize fields can cause great damage to certain maize inbred lines and hybrids. Two maize genotypes, tolerant inbred(HBR) and sensitive inbred(HBS), were fo... Postemergence application of nicosulfuron for weed control in maize fields can cause great damage to certain maize inbred lines and hybrids. Two maize genotypes, tolerant inbred(HBR) and sensitive inbred(HBS), were found to significantly differ in their phenotypic responses to nicosulfuron, with the EC50(50% effective concentration) values differed statistically(763.6 and 5.9 g a.i. ha–1, respectively). Pre-treatment with malathion, a known cytochrome P450 inhibitor, increased nicosulfuron injury in both HBR and HBS. Our results support the hypothesis that nicosulfuron selectivity in maize is associated with cytochrome P450 metabolism. Further analysis of the maize genome resulted in the identification of 314 full length cytochrome P450 monooxygenase(CYP) genes. These genes were classified into 2 types, 10 clans and 44 families. The CYP71 clan was represented by all A-type genes(168) belonging to 17 families. Nine clans possessed 27 families containing 146 non-A-type genes. The consensus sequences of the heme-binding regions of A-type and non-A-type CYP proteins are ‘PFGXGRRXCPG’ and ‘FXXGPRXCXG’, respectively. Illumina transcriptome sequence results showed that there were 53 differentially expressed CYP genes on the basis of high variation in expression between HBS and HBR, nicosulfuron-treated and untreated samples. These genes may contribute to nicosulfuron tolerance in maize. A hierarchical clustering analysis obtained four main clusters named C1 to C4 in which 4, 15, 21, and 13 CYP genes were found in each respective cluster. The expression patterns of some CYP genes were confirmed by RT-q PCR analysis. The research will improve our understanding of the function of maize cytochrome P450 in herbicide metabolism. 展开更多
关键词 CYTOCHROME P450 Zea mays L. MALATHION NICOSULFURON HERBICIDE metabolism
在线阅读 下载PDF
上一页 1 2 191 下一页 到第
使用帮助 返回顶部 意见反馈