期刊文献+
共找到13,911篇文章
< 1 2 250 >
每页显示 20 50 100
Action of trichostatin A on Alzheimer’s disease-like pathological changes in SH-SY5Y neuroblastoma cells 预览
1
作者 Li-Hua Li Wen-Na Peng +2 位作者 Yu Deng1 Jing-Jing Li Xiang-Rong Tian 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期293-301,共9页
The histone deacetylase inhibitor, trichostatin A, is used to treat Alzheimer’s disease and can improve learning and memory but its underlying mechanism of action is unknown. To determine whether the therapeutic effe... The histone deacetylase inhibitor, trichostatin A, is used to treat Alzheimer’s disease and can improve learning and memory but its underlying mechanism of action is unknown. To determine whether the therapeutic effect of trichostatin A on Alzheimer’s disease is associated with the nuclear factor erythroid 2-related factor 2(Nrf2) and Kelch-like epichlorohydrin-related protein-1(Keap1) signaling pathway, amyloid β-peptide 25–35(Aβ25–35) was used to induce Alzheimer’s disease-like pathological changes in SH-SY5 Y neuroblastoma cells. Cells were then treated with trichostatin A. The effects of trichostatin A on the expression of Keap1 and Nrf2 were detected by real-time quantitative polymerase chain reaction, western blot assays and immunofluorescence. Total antioxidant capacity and autophagy activity were evaluated by total antioxidant capacity assay kit and light chain 3-I/II levels, respectively. We found that trichostatin A increased cell viability and Nrf2 expression, and decreased Keap1 expression in SH-SY5 Y cells. Furthermore, trichostatin A increased the expression of Nrf2-related target genes, such as superoxide dismutase, NAD(P)H quinone dehydrogenase 1 and glutathione S-transferase, thereby increasing the total antioxidant capacity of SH-SY5 Y cells and inhibiting amyloid β-peptide-induced autophagy. Knockdown of Keap1 in SH-SY5 Y cells further increased trichostatin A-induced Nrf2 expression. These results indicate that the therapeutic effect of trichostatin A on Alzheimer’s disease is associated with the Keap1-Nrf2 pathway. The mechanism for this action may be that trichostatin A increases cell viability and the antioxidant capacity of SH-SY5 Y cells by alleviating Keap1-mediated inhibition Nrf2 signaling, thereby alleviating amyloid β-peptide-induced cell damage. 展开更多
关键词 Alzheimer's disease amyloid-β peptide autophagy KEAP1 signal neurocytotoxicity oxidative stress damage SH-SY5Y cells total antioxidant capacity transcription factor Nrf2 TSA
在线阅读 下载PDF
Is there a relationship between dopamine and rhegmatogenous retinal detachment? 预览
2
作者 Alessio Martucci Massimo Cesareo +5 位作者 Maria Dolores Pinazo-Durán Michela Di Pierro Matteo Di Marino Carlo Nucci Massimiliano Coletta Raffaele Mancino 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期311-314,共4页
Dopamine and its receptors have been widely studied in the neurological conditions and in the retina. In this study, we evaluated the possible role of dopamine in rhegmatogenous retinal detachment(RRD) by comparing th... Dopamine and its receptors have been widely studied in the neurological conditions and in the retina. In this study, we evaluated the possible role of dopamine in rhegmatogenous retinal detachment(RRD) by comparing the amount of 3,4-dihydroxyphenylacetic acid(DOPAC), a surrogate index of retinal dopamin levels, in the vitreous sample of patients affected by RRD with those affected by macular pucker and vitre ous hemorrhage. Our results showed that significantly higher levels of DOPAC were found in the vitreou sample of patients affected by RRD compared with those affected by vitreous hemorrhage and macula pucker(P = 0.002). Specifically, no trace of the substance was found in vitreous hemorrhage and macula pucker samples. A slightly significant positive correlation was found among DOPAC and post-operativ best corrected visual acuity(r = 0.470, P = 0.049). No correlation was found between DOPAC and the day elapsed between diagnosis and surgery(P = 0.317). For the first time our findings suggest that DOPAC i released in RRD, but not in other retinal diseases such as vitreous hemorrhage and macular pucker. More over, we showed a correlation between visual acuity outcome and the amount of DOPAC in the vitreous This might have a potential, although still unknown, implication in the pathogenesis of the disease and/o in the associated photoreceptors loss. This study was approved by the Ethics Committee of Rome Tor Ver gata University Hospital(R.S.92.10) on September 24, 2010. 展开更多
关键词 3 4-dihydroxyphenylacetic acid DOPAC DOPAMINE DOPAMINERGIC amacrine cells DOPAMINERGIC neurotoxicity MACULAR pucker oxidative stress PHOTORECEPTOR degeneration rhegmatogenous retinal DETACHMENT VITREOUS hemorrhage
在线阅读 下载PDF
Neuroinflammation and oxidative stress act in concert to promote neurodegeneration in the diabetic retina and optic nerve:galectin-3 participation 预览
3
作者 Henrique Rocha Mendonca Raul Carpi-Santos +1 位作者 Karin da Costa Calaza Ana Maria Blanco Martinez 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期625-635,共11页
Diabetes is a lifelong disease characterized by glucose metabolic imbalance,in which low insulin levels or impaired insulin signaling lead to hyperglycemic state.Within 20 years of diabetes progression,95%of patients ... Diabetes is a lifelong disease characterized by glucose metabolic imbalance,in which low insulin levels or impaired insulin signaling lead to hyperglycemic state.Within 20 years of diabetes progression,95%of patients will have diabetic retinopathy,the leading cause of visual defects in working-age people worldwide.Although diabetes is considered a microvascular disease,recent studies have shown that neurodegeneration precedes vascular changes within the diabetic visual system,albeit its mechanisms are still under investigation.Neuroinflammation and oxidative stress are intrinsically related phenomena,since macrophage/microglia and astrocytes are the main sources of reactive oxygen species during central nervous system chronic degenerative diseases,and both pathological processes are increased in the visual system during diabetes.The present review will focus on recent findings of the contribution of oxidative stress derived from neuroinflammation in the early neurodegenerative aspects of the diabetic visual system and their relationship with galectin-3. 展开更多
关键词 diabetes diabetic RETINOPATHY GALECTIN-3 NEURODEGENERATION NEUROINFLAMMATION optic nerve oxidative stress retina
在线阅读 下载PDF
Therapeutic importance of hydrogen sulfide in age-associated neurodegenerative diseases 预览
4
作者 Rubaiya Tabassum Na Young Jeong Junyang Jung 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期653-662,共10页
Hydrogen sulfide(H2S)is a gasotransmitter that acts as an antioxidant and exhibits a wide variety of cytoprotective and physiological functions in age-associated diseases.One of the major causes of age-related disease... Hydrogen sulfide(H2S)is a gasotransmitter that acts as an antioxidant and exhibits a wide variety of cytoprotective and physiological functions in age-associated diseases.One of the major causes of age-related diseases is oxidative stress.In recent years,the importance of H2S has become clear,although its antioxidant function has not yet been fully explored.The enzymes cystathionineβ-synthase,cystathionineγ-lya-se,and 3-mercaptopyruvate sulfurtransferase are involved in the enzymatic production of H2S.Previously,H2S was considered a neuromodulator,given its role in long-term hippocampal potentiation,but it is now also recognized as an antioxidant in age-related neurodegeneration.Due to aerobic metabolism,the central nervous system is vulnerable to oxidative stress in brain aging,resulting in age-associated degenerative diseases.H2S exerts its antioxidant effect by limiting free radical reactions through the activation of antioxidant enzymes,including superoxide dismutase,catalase,and glutathione peroxidase,which protect against the effects of aging by regulating apoptosis-related genes,including p53,Bax,and Bcl-2.This review explores the implications and mechanisms of H2S as an antioxidant in age-associated neurodegenerative diseases,including Alzheimer’s disease,Parkinson’s disease,Huntington’s disease,and Down syndrome. 展开更多
关键词 3-mercaptopyruvate SULFURTRANSFERASE aging antioxidant cystathionineβ-synthase cystathionineγ-lyase GLUTATHIONE hydrogen sulfide NEURODEGENERATIVE disease oxidative stress reactive oxygen species
在线阅读 下载PDF
Adenylate cyclase activator forskolin alleviates intracerebroventricular propionic acid-induced mitochondrial dysfunction of autistic rats 预览
5
作者 Sidharth Mehan Saloni Rahi +7 位作者 Aarti Tiwari Tarun Kapoor Kajal Rajdev Ramit Sharma Himanshi Khera Sourabh Kosey Umesh Kukkar Rajesh Dudi 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第6期1140-1149,共10页
Neuronal mitochondrial dysfunction increases inflammatory mediators and leads to free radical generation and anti-oxidant enzymatic alterations,which are major neuropathological hallmarks responsible for autism.Mitoch... Neuronal mitochondrial dysfunction increases inflammatory mediators and leads to free radical generation and anti-oxidant enzymatic alterations,which are major neuropathological hallmarks responsible for autism.Mitochondrial dysfunction in autism is associated with decreased ATP levels due to reduced levels of cyclic adenosine monophosphate.Rat models of autism were established by intracerebroventricular injection of propionic acid.These rat models had memory dysfunction,decreased muscle coordination and gait imbalance.Biochemical estimation of propionic acid-treated rats showed changes in enzyme activity in neuronal mitochondrial electron transport chain complexes and increases in pro-inflammatory cytokines,oxidative stress and lipid biomarkers.Oral administration of 10,20 and 30 mg/kg adenylate cyclase activator forskolin for 15 days reversed these changes in a dose-dependent manner.These findings suggest that forskolin can alleviate neuronal mitochondrial dysfunction and improve neurological symptoms of rats with autism.This study was approved by the RITS/IAEC,SIRSA,HARYANA on March 3,2014(approval No.RITS/IAEC/2014/03/03). 展开更多
关键词 adenylate cyclase ATP AUTISM FORSKOLIN mitochondrial dysfunction NEUROINFLAMMATION oxidative stress propionic acid
在线阅读 下载PDF
Protective effect of hydrogen sulfide on oxidative stress-induced neurodegenerative diseases 预览
6
作者 Rubaiya Tabassum Na Young Jeong Junyang Jung 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期232-241,共10页
Hydrogen sulfide is an antioxidant molecule that has a wide range of biological effects against oxidative stress. Balanced oxidative stress is also vital for maintaining cellular function in biological system, where r... Hydrogen sulfide is an antioxidant molecule that has a wide range of biological effects against oxidative stress. Balanced oxidative stress is also vital for maintaining cellular function in biological system, where reactive oxygen species are the main source of oxidative stress. When the normal redox balance is disturbed, deoxyribonucleic acid, lipid, and protein molecules are oxidized under pathological conditions, like diabetes mellitus that leads to diabetic peripheral neuropathy. In diabetes mellitus-induced diabetic peripheral neuropathy, due to hyperglycemia, pancreatic beta cell(β cell) shows resistance to insulin secretion. As a consequence, glucose metabolism is disturbed in neuronal cells which are distracted from providing proper cell signaling pathway. Not only diabetic peripheral neuropathy but also other central damages occur in brain neuropathy. Neurological studies regarding type 1 diabetes mellitus patients with Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis have shown changes in the central nervous system because high blood glucose levels(HbA1 c) appeared with poor cognitive function. Oxidative stress plays a role in inhibiting insulin signaling that is necessary for brain function. Hydrogen sulfide exhibits antioxidant effects against oxidative stress, where cystathionine β synthase, cystathionine γ lyase, and 3-mercaptopyruvate sulfurtransferase are the endogenous sources of hydrogen sulfide. This review is to explore the pathogenesis of diabetes mellitus-induced diabetic peripheral neuropathy and other neurological comorbid disorders under the oxidative stress condition and the anti-oxidative effects of hydrogen sulfide. 展开更多
关键词 Alzheimer's DISEASE amyotrophic lateral SCLEROSIS antioxidant diabetic peripheral NEUROPATHY DNA oxidation hydrogen SULFIDE mitochondrial dysfunction oxidative stress Parkinson's DISEASE reactive oxygen species
在线阅读 下载PDF
Microglial cathepsin B as a key driver of inflammatory brain diseases and brain aging 预览
7
作者 Hiroshi Nakanishi 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第1期25-29,共5页
Interleukin-1βis a potent proinflammatory cytokine that plays a key role in the pathogenesis of the brain aging and diverse range of neurological diseases including Alzheimer’s disease,Parkinson’s disease,stroke an... Interleukin-1βis a potent proinflammatory cytokine that plays a key role in the pathogenesis of the brain aging and diverse range of neurological diseases including Alzheimer’s disease,Parkinson’s disease,stroke and persistent pain.Activated microglia are the main cellular source of interleukin-1βin the brain.Cathepsin B is associated with the production and secretion of interleukin-1βthrough pyrin domain-containing protein 3 inflammasome-independent processing of procaspase-3 in the phagolysosomes.The leakage of cathepsin B from the endosomal-lysosomal system during aging is associated with the proteolytic degradation of mitochondrial transcription factor A,which can stabilize mitochondrial DNA.Therefore,microglial cathepsin B could function as a major driver for inflammatory brain diseases and brain aging.Orally active and blood-brain barrier-permeable specific inhibitors for cathepsin B can be potentially effective new pharmaceutical interventions against inflammatory brain diseases and brain aging. 展开更多
关键词 BRAIN aging caspase-1 CATHEPSIN B INFLAMMATORY BRAIN diseases INTERLEUKIN-1Β microglia mitochondrial transcription FACTOR A neuroinflammation nuclear factor-κB oxidative stress
在线阅读 下载PDF
脑心清对脑缺血再灌注损伤模型大鼠的保护机制 预览
8
作者 闵冬雨 李红岩 +5 位作者 关乐 常江 张海宁 崔馨月 王鹏 曹永刚 《中国组织工程研究》 CAS 北大核心 2020年第2期215-222,共8页
背景:脑心清胶囊用于脑缺血再灌注损伤的治疗由来已久,然而针对其作用机制的深入研究则相对较少。目的:应用分子生物学手段考察脑心清胶囊对脑缺血再灌注损伤沙鼠模型的治疗作用。方法:实验方案经辽宁中医药大学动物实验伦理委员会批准... 背景:脑心清胶囊用于脑缺血再灌注损伤的治疗由来已久,然而针对其作用机制的深入研究则相对较少。目的:应用分子生物学手段考察脑心清胶囊对脑缺血再灌注损伤沙鼠模型的治疗作用。方法:实验方案经辽宁中医药大学动物实验伦理委员会批准(批准号为21000092017072)。将80只雄性蒙古沙鼠随机分为假手术组、模型组、脑心清组及脑络通组,后3组沙鼠应用无创微动脉夹同时夹闭双侧颈总动脉5 min后松开,建立脑缺血再灌注损伤模型;假手术组不夹闭双侧颈总动脉。术后次日开始假手术组正常饲养,模型组灌服同体积的生理盐水,脑心清组按照100 mg/(kg?d)灌胃给药,脑络通组按照100 mg/(kg?d)灌胃给药,连续给药21 d。在实验结束前1周进行水迷宫实验,实验结束后麻醉下处死沙鼠取脑组织。检测沙鼠的学习记忆功能、海马神经元、脑血管及对应的分子变化情况。结果与结论:①同假手术组相比,模型组沙鼠学习能力显著下降。而脑心清组及脑络通组则可有效提升术后的学习能力下降趋势;②与模型组相比,脑心清组及脑络通组神经元显著增多,且排列较为整齐,细胞轮廓清晰,结构完整;③与模型组相比,脑心清组及脑络通组沙鼠的超氧化物歧化酶和乳酸脱氢酶活性,谷胱甘肽含量显著升高,丙二醛含量显著降低(P<0.01);④与模型组相比,脑心清组及脑络通组沙鼠海马组织ASC、NLRP3和Caspase-1蛋白表达下调(P<0.05);⑤与模型组相比,脑心清组及脑络通组沙鼠的白细胞介素18和白细胞介素1β含量明显降低(P<0.01);⑥与模型组相比,脑心清组及脑络通组沙鼠的血小板内皮细胞黏附分子1阳性细胞明显增多,细胞间连接紧密;⑦与模型组相比,脑心清组及脑络通组沙鼠海马组织血小板内皮细胞黏附分子1和磷酸化内皮型一氧化氮合酶表达显著上调,一氧化氮含量显著升高(P<0.01);⑧结果说明,脑心 展开更多
关键词 脑心清胶囊 脑缺血再灌注 氧化应激 炎症 脑血管功能 细胞焦亡
在线阅读 下载PDF
左归丸对成骨细胞氧化应激损伤的保护作用 预览
9
作者 乔久涛 关德宏 +1 位作者 王冬艳 刘艾芸 《中国组织工程研究》 CAS 北大核心 2020年第7期1052-1056,共5页
背景:最新研究发现氧化应激在骨质疏松症中发挥着重要作用,衰老、雌激素缺乏、核因子E2相关因子2(Nrf2)、血红素加氧酶1与活性氧的产生和骨质疏松发病有着重要的关系。目的:分析左归丸对成骨细胞氧化应激损伤的保护作用机制。方法:实验... 背景:最新研究发现氧化应激在骨质疏松症中发挥着重要作用,衰老、雌激素缺乏、核因子E2相关因子2(Nrf2)、血红素加氧酶1与活性氧的产生和骨质疏松发病有着重要的关系。目的:分析左归丸对成骨细胞氧化应激损伤的保护作用机制。方法:实验方案经哈尔滨医科大学附属第二医院伦理委员会批准。分离培养SD乳鼠颅骨成骨细胞,实验分为空白组、模型组(300μmol/L H2O2)、左归丸组(300μmol/L H2O2+10%左归丸含药血清)。用H2O2建立成骨细胞氧化应激损伤模型;在成骨细胞氧化应激损伤同时,用左归丸含药血清培养成骨细胞,空白组不做任何处理。测定各组成骨细胞中丙二醛和超氧化物歧化酶含量;Western blot检测成骨细胞中核因子E2相关因子2和血红素加氧酶1蛋白表达。结果与结论:①与空白组比较,模型组中丙二醛含量明显升高,超氧化物歧化酶的含量明显降低;与模型组比较,左归丸组中丙二醛含量明显降低,超氧化物歧化酶的含量明显升高;②与空白组比较,模型组成骨细胞中血红素加氧酶1和核因子E2相关因子2表达明显增加;左归丸组成骨细胞中血红素加氧酶1和核因子E2相关因子2表达明显高于模型组;③结果说明,左归丸对成骨细胞氧化损伤具有保护作用。 展开更多
关键词 左归丸 成骨细胞 NRF2 HO-1 血红素加氧酶1 超氧化物歧化酶 丙二醛 氧化应激
在线阅读 下载PDF
N-乙酰半胱氨酸对烟熏模型大鼠肺组织氧化应激的影响 预览
10
作者 李响 王玮 +1 位作者 夏书月 何巍 《中国组织工程研究》 CAS 北大核心 2020年第2期254-259,共6页
背景:N-乙酰半胱氨酸是谷胱甘肽的前体,可以直接清除氧自由基。然而,N-乙酰半胱氨酸是否通过降低氧化应激反应,减轻吸烟导致的肺损伤尚不完全清楚。目的:探讨N-乙酰半胱氨酸对烟熏大鼠肺组织氧化应激的影响,并阐明其可能的作用机制。方... 背景:N-乙酰半胱氨酸是谷胱甘肽的前体,可以直接清除氧自由基。然而,N-乙酰半胱氨酸是否通过降低氧化应激反应,减轻吸烟导致的肺损伤尚不完全清楚。目的:探讨N-乙酰半胱氨酸对烟熏大鼠肺组织氧化应激的影响,并阐明其可能的作用机制。方法:将30只雄性大鼠随机分为对照组、烟熏组、烟熏+N-乙酰半胱氨酸组。将烟熏组和烟熏+N-乙酰半胱氨酸组大鼠置于被动吸烟动物染毒系统,每次暴露20支香烟的烟雾中,2次/d,1 h/次,持续8周。N-乙酰半胱氨酸组大鼠每天被动烟熏前给予N-乙酰半胱氨酸200 mg/kg灌胃,持续8周。正常对照组大鼠仅单纯放置在染毒系统内,正常饲养8周。实验方案由沈阳医学院实验动物伦理委员会于2018年10月批准,批准号:研伦审第(2018)85号。结果与结论:①病理观察发现烟熏组大鼠肺组织排列紊乱、肺泡间隔增厚、炎症细胞浸润和间质纤维化,而烟熏+N-乙酰半胱氨酸组肺组织出血、间质内炎症细胞数量显著减少、纤维化程度减轻;②与对照组相比,烟熏组大鼠肺组织丙二醛5和肌醇酶α基因表达升高,超氧化物歧化酶1基因表达显著降低,而N-乙酰半胱氨酸可抑制上述变化;③免疫荧光和Western blot结果均发现烟熏+N-乙酰半胱氨酸组大鼠肺组织丙二醛5和肌醇酶α蛋白表达较烟熏组显著降低,超氧化物歧化酶1蛋白表达较烟熏组升高。此外,烟熏+N-乙酰半胱氨酸组核因子E2相关因子和Keap1蛋白表达明显升高,Bach1蛋白表达显著降低;④结果表明,N-乙酰半胱氨酸通过降低氧化应激对烟熏导致的肺损伤发挥保护作用,其作用机制可能通过激活核因子E2相关因子/Keap1信号通路实现。 展开更多
关键词 烟熏 N-乙酰半胱氨酸 同型半胱氨酸 氧化应激 核因子E2相关因子/Keap1信号通路 肺损伤 丙二醛5 肌醇酶α
在线阅读 下载PDF
Highlights of ASS234:a novel and promising therapeutic agent for Alzheimer’s disease therapy 预览
11
作者 Alejandro Romero JoséMarco-Contelles Eva Ramos 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第1期30-35,共6页
There is no effective treatment to face Alzheimer’s disease complexity.Multitarget molecules are a good approach against the multiple physiopathological events associated with its development and progression.In this ... There is no effective treatment to face Alzheimer’s disease complexity.Multitarget molecules are a good approach against the multiple physiopathological events associated with its development and progression.In this context,N-((5-(3-(1-benzylpiperidin-4-yl)propoxy)-1-methyl-1H-indol-2-yl)methyl)-N-methylprop-2-yn-1-amine(ASS234)has been tested achieving promising results.ASS234 has demonstrated to cross the blood-brain barrier in vivo,and a good in silico safety profile being less toxic than donepezil.Besides,ASS234 reversibly inhibits human acetyl-and butyryl-cholinesterase,and irreversibly inhibits human monoamine oxidase A and B.Moreover,this multitarget molecule has antioxidant and neuroprotective properties,and inhibitsΑβ1–42 andΑβ1–40 self-aggregation.Inquiring about the mechanism of action,several signaling pathways related to Alzheimer’s disease had been explored showing that ASS234 induces the wingless-type MMTV integration site(Wnt)family and several members of the heat shock proteins family and moreover counteracts neuroinflammatory and oxidative stress-related genes promoting the induction of several key antioxidant genes.Finally,in vivo experiments with ASS234 in C57BL/6J mice displayed its ability to reduce amyloid plaque burden and gliosis in the cortex and hippocampus,ameliorating scopolamine-induced learning deficits.Here we gather the information regarding ASS234 evaluated so far,showing its ability to face different targets,necessary to counteract a neurodegenerative disease as complex as the Alzheimer’s disease. 展开更多
关键词 ACHE BuChE gene expression heat shock proteins inflammation in silico TOXICOLOGY MAO A/B NEUROPROTECTION oxidative stress Wnt signaling
在线阅读 下载PDF
Dietary habits,lifestyle factors and neurodegenerative diseases 预览
12
作者 Aurel Popa-Wagner Dinu Iuliu Dumitrascu +4 位作者 Bogdan Capitanescu Eugen Bogdan Petcu Roxana Surugiu Wen-Hui Fang Danut-Adrian Dumbrava 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期394-400,共7页
Worldwide stroke is increasing in parallel with modernization,changes in lifestyle,and the growing elderly population.Our review is focused on the link between diet,as part of‘modern lifestyle’,and health in the con... Worldwide stroke is increasing in parallel with modernization,changes in lifestyle,and the growing elderly population.Our review is focused on the link between diet,as part of‘modern lifestyle’,and health in the context of genetic predisposition of individuals to‘unhealthy’metabolic pathway activity.It is concluded that lifestyle including high sugar diets,alcohol and tobacco addiction or high fat diets as well as ageing,brain injury,oxidative stress and neuroinflammation,negatively influence the onset,severity and duration of neurodegenerative diseases.Fortunately,there are several healthy dietary components such as polyunsaturated fatty acids and the anti-oxidants curcumin,resveratrol,blueberry polyphenols,sulphoraphane,salvionic acid as well as caloric restriction and physical activity,which may counteract ageing and associated neurodegenerative diseases via increased autophagy or increased neurogenesis in the adult brain. 展开更多
关键词 brain injury DIETARY HABITS LIFESTYLE metaflammation NEURODEGENERATION OXIDATIVE stress type 2 diabetes MELLITUS
在线阅读 下载PDF
桑黄粗多糖对被动吸烟模型小鼠运动能力和骨骼肌自由基代谢的影响 预览
13
作者 万华喆 柴广新 +1 位作者 肖晓玲 黄文英 《中国组织工程研究》 CAS 北大核心 2020年第5期689-693,共5页
背景:香烟烟雾中含有大量多种类型氧自由基和细胞毒性成分,被动吸烟会损害呼吸、心血管系统功能,导致骨骼肌氧化损伤和运动能力下降。目的:观察具有抗氧化应激作用的桑黄粗多糖对被动吸烟模型小鼠运动能力和骨骼肌氧化应激的影响,为被... 背景:香烟烟雾中含有大量多种类型氧自由基和细胞毒性成分,被动吸烟会损害呼吸、心血管系统功能,导致骨骼肌氧化损伤和运动能力下降。目的:观察具有抗氧化应激作用的桑黄粗多糖对被动吸烟模型小鼠运动能力和骨骼肌氧化应激的影响,为被动吸烟小鼠骨骼肌过氧化损伤和运动能力下降的防治提供思路。方法:将21只雄性昆明小鼠随机分为3组:桑黄组采用桑黄粗多糖灌胃然后被动吸烟,对照组采用蒸馏水灌胃然后被动吸烟,空白组单纯蒸馏水灌胃。各组干预持续4周,然后进行1次力竭游泳运动,记录各组小鼠游泳力竭时间。运动结束后检测小鼠双侧腓肠肌超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶与Ca^2+-Mg^2+-ATP、Na^+-K^+-ATP酶活性及丙二醛含量。结果与结论:(1)与空白组相比,对照组小鼠力竭游泳时间缩短(P<0.01);与对照组相比,桑黄组小鼠力竭游泳时间延长(P<0.01);(2)与空白组相比,对照组小鼠超氧化物歧化酶、谷胱甘肽还原酶、过氧化氢酶活性和Ca^2+-Mg^2+-ATP、Na+-K+-ATP酶活性显著降低(P<0.01),丙二醛含量显著升高(P<0.01);(3)与对照组相比,桑黄组小鼠超氧化物歧化酶、谷胱甘肽还原酶、过氧化氢酶活性和Ca^2+-Mg^2+-ATP、Na^+-K^+-ATP活性升高(P<0.05,P<0.01),丙二醛含量显著降低(P<0.01);(4)相关性分析显示:力竭游泳时间与谷胱甘肽还原酶、过氧化氢酶、超氧化物歧化酶活性呈正相关(r>0.6,P<0.05),与丙二醛呈负相关(r=-0.637,P<0.05);Ca^2+-Mg^2+-ATP、Na^+-K^+-ATP酶活性与谷胱甘肽还原酶、过氧化氢酶及超氧化物歧化酶活性呈正相关(r> 0.6,P<0.05),与丙二醛呈负相关(r<-0.6,P<0.05);(5)结果提示:桑黄粗多糖可以提高被动吸烟模型小鼠骨骼肌抗氧化酶活性,减轻脂质过氧化反应,提高运动能力。实验方案经江西师范大学体育综合实验中心实验室伦理委员会批准(批准号:201703)。 展开更多
关键词 桑黄粗多糖 灌胃 小鼠 被动吸烟 氧化应激 脂质过氧化反应 抗氧化酶 骨骼肌 运动能力 相关性分析
在线阅读 下载PDF
Neuroprotection of Cyperus esculentus L.orientin against cerebral ischemia/reperfusion induced brain injury 预览
14
作者 Si-Qun Jing Sai-Sai Wang +5 位作者 Rui-Min Zhong Jun-Yan Zhang Jin-Zi Wu Yi-Xian Tu Yan Pu Liang-Jun Yan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期548-556,共9页
Orientin is a flavonoid monomer.In recent years,its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia,anti-apoptosis,anti-radiation,an... Orientin is a flavonoid monomer.In recent years,its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia,anti-apoptosis,anti-radiation,anti-tumor,and anti-aging.However,the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated.The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L.orientin(CLO)from Cyperus esculentus L.leaves against ischemia/reperfusion(I/R)injury using standard orientin as control.For in vitro studies,we treated HT22 cells with CoCl2 as an in vitro ischemic injury model.HT22 cells in the control group were treated with CoCl2.For in vivo studies,we used rat models of middle cerebral artery occlusion,and animals that received sham surgery were used as controls.We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation.However,CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase.Results showed that CLO could decrease neurological deficit score,attenuate brain water content,and reduce cerebral infarct volume,leading to neuroprotection during cerebral ischemia-reperfusion injury.Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry.The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3.All experimental procedures and protocols were approved on May 16,2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China(approval No.IACUC20160516-57). 展开更多
关键词 ANTIOXIDANTS caspase-3 cerebral ISCHEMIA/REPERFUSION injury cobalt chloride CYPERUS esculentus L.orientin(CLO) lipid PEROXIDATION nerve regeneration NEUROLOGICAL deficits oxidative stress reactive oxygen species
在线阅读 下载PDF
A mimetic peptide ofα2,6-sialyllactose promotes neuritogenesis 预览
15
作者 Shuang-Xi Chen Jia-Hui He +3 位作者 Yong-Jian Mi Hui-Fan Shen Melitta Schachner Wei-Jiang Zhao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第6期1058-1065,共8页
Oxidative stress contributes to the pathogenesis of neurodegenerative diseases.With the aim to find reagents that reduce oxidative stress,a phage display library was screened for peptides mimicking a2,6-sialyllactose(... Oxidative stress contributes to the pathogenesis of neurodegenerative diseases.With the aim to find reagents that reduce oxidative stress,a phage display library was screened for peptides mimicking a2,6-sialyllactose(6'-SL),which is known to beneficially influence neural functions.Using Sambucus nigra lectin,which specifically binds to 6'-SL,we screened a phage display library and found a peptide comprising identical sequences of 12 amino acids.Mimetic peptide,reverse peptide and scrambled peptide were tested for inhibition of 6'-SL binding to the lectin.Indeed,lectin binding to 6'-SL was inhibited by the most frequently identified mimetic peptide,but not by the reverse or scrambled peptides,showing that this peptide mimics 6'-SL.Functionally,mimetic peptide,but not the reverse or scrambled peptides,increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells,and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H_20_2-induced oxidative stress.The combined results indicate that the 6'-SL mimetic peptide promotes neuronal survival and neuritogenesis,thus raising hopes for the treatment of neurodegenerative diseases.This study was approved by the Medical Ethics Committee of Shantou University Medical College,China(approval No.SUMC 2014-004)on February 20,2014. 展开更多
关键词 central nervous system cerebellar granule neurons mimetic peptide neural cell adhesion molecule L1 NEURITOGENESIS neurodegenerative disease neuronal survival oxidative stress phage display Sambucus nigra lectin α2 6-sialyllactose
在线阅读 下载PDF
Effect of Exhausting Exercise on Oxidative Stress in Health, Hemodialysis and Professional Sport 预览
16
作者 Knap Bojan Prezelj Marija Ve?eri?-Haler ?eljka 《自然科学期刊(英文)》 2019年第11期307-314,共8页
The aim of presented study is to compare parameters of oxidative stress in untrained volunteers, patients with chronic, non-motor related disease (dialysis patients) and professional athletes before and after exhausti... The aim of presented study is to compare parameters of oxidative stress in untrained volunteers, patients with chronic, non-motor related disease (dialysis patients) and professional athletes before and after exhaustive exercise. 40 subjects participated in the study: 14 healthy, untrained subjects, 12 hemodialysis patients and 14 professional rowers. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and non-transferrin bound iron (NTBI) were determined before and after exercise. Dialysis patients have increased oxidative stress at rest with highest NTBI, and show adaptation with increased values of GPx and decreased SOD. Professional athletes have low level of oxidative stress at basic circumstances with lowest NTBI, SOD, CAT and GPx as compared to untrained volunteers and dialysis patients. After strenuous exercise elevation of antioxidative enzymes is observed only in athletes, but not in untrained and dialysis patients. Due to limited antioxidative capacity, extreme physical effort is probably not recommended to dialysis patients and untrained people. 展开更多
关键词 HEMODIALYSIS OXIDATIVE STRESS Strenuous EXERCISE ROWERS OXIDATIVE STRESS Athletes OXIDATIVE STRESS DIALYSIS
在线阅读 免费下载
代谢组学在动物应激研究中的应用
17
作者 李文杰 连帅 +1 位作者 王立鹏(综述) 杨焕民(审校) 《中国生物制品学杂志》 CAS CSCD 2019年第4期482-484,488共4页
代谢组学是生物机体在病理生理、遗传、环境或行为的不同刺激下,对机体所产生代谢产物变化的研究,是生物领域研究中一种重要的研究手段,已在植物学、营养学、中药学、微生物学、临床疾病诊断等研究领域得到广泛应用。动物应激一直是畜... 代谢组学是生物机体在病理生理、遗传、环境或行为的不同刺激下,对机体所产生代谢产物变化的研究,是生物领域研究中一种重要的研究手段,已在植物学、营养学、中药学、微生物学、临床疾病诊断等研究领域得到广泛应用。动物应激一直是畜牧行业关注的热点,许多因素可导致动物应激,常见的应激类型有冷应激、热应激、氧化应激、束缚应激、噪音应激、运输应激,还有不可预知的慢性应激等,这些应激均可对动物机体产生一定危害。目前,代谢组学也已应用于动物应激领域的研究,本文对代谢组学在动物的冷应激、热应激、氧化应激及束缚应激研究中的应用作一综述。 展开更多
关键词 代谢组学 冷应激 热应激 氧化应激 束缚应激
动物应激模型的构建与应用
18
作者 刘波 吕宏伟 +2 位作者 陈雅湘 穆蕊 方热军 《饲料研究》 CAS 北大核心 2019年第1期114-117,共4页
应激是动物受到病原性和非病原性抗原的刺激时产生精神萎靡、食欲下降,嗜睡、生长缓慢等现象。因此,构建可靠的动物应激模型,对于揭示动物应激机制及研制抗应激添加剂具有重要意义。主要综述了应激模型的构建方法及其在动物生产中的应用... 应激是动物受到病原性和非病原性抗原的刺激时产生精神萎靡、食欲下降,嗜睡、生长缓慢等现象。因此,构建可靠的动物应激模型,对于揭示动物应激机制及研制抗应激添加剂具有重要意义。主要综述了应激模型的构建方法及其在动物生产中的应用,以期为动物生产中应激防治提供依据。 展开更多
关键词 免疫应激 氧化应激 冷应激 热应激
Anthocyanin-Rich Blueberry Extract Ameliorates the Behavioral Deficits of MPTP-Induced Mouse Model of Parkinson’s Disease via Anti-Oxidative Mechanisms 预览
19
作者 Feng Qian Mengmeng Wang +1 位作者 Jianggang Wang Chengbiao Lu 《长江医药(英文)》 2019年第1期72-78,共7页
The pathological process of Parkinson’s disease (PD) involves oxidative stress, mitochondrial dysfunction and dopaminergic neuronal loss. Recent studies have suggested that the fruits rich in anthocyanins (ANC) were ... The pathological process of Parkinson’s disease (PD) involves oxidative stress, mitochondrial dysfunction and dopaminergic neuronal loss. Recent studies have suggested that the fruits rich in anthocyanins (ANC) were neuroprotective, and then could reduce the risk of PD. This study was designed to investigate the effects of the ANC rich blueberry extracts (BBE) on the behavior and the oxidative stress in the mouse model of PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). After 3 weeks of BBE administration, we monitored the behavioral alterations and measured the level of dopamine (DA), enzymatic tyrosine hydroxylase (TH), non-enzymatic malonaldehyde (MDA), enzymatic superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in the brain and serum. Our results demonstrated that the behavioral impairment, the increased MDA level and the decreased SOD and GSH-PX activities in the MPTP-induced PD mice, were significantly attenuated by BBE administration (p < 0.05). This study suggested that anthocyanin-rich BBE should have neuroprotective effects through the anti-oxidative mechanisms. 展开更多
关键词 BLUEBERRY EXTRACT Parkinson’s Disease Oxidative Stress
在线阅读 免费下载
Seaberry (<i>Hippophae rhamnoides L.</i>) and Water Lily (<i>Nymphaeaceae</i>) Extracts Protect Human Skin against Blue Light, Environmental Pollutants and UV-A Irradiations in an <i>Ex Vivo</i>Model System 预览
20
作者 Softa Mohamed Percoco Giuseppe +3 位作者 Almeida Scalvino Stéphanie Peno-Mazzarino Laurent Pauline Bony Lati Elian 《化妆品、皮肤病及应用期刊(英文)》 2019年第3期244-262,共19页
Background: The skin is the outer shell of the mammalian body and it is continuously exposed to a large spectrum of external stimuli including sun irradiation and atmospheric pollutants. These factors present deleteri... Background: The skin is the outer shell of the mammalian body and it is continuously exposed to a large spectrum of external stimuli including sun irradiation and atmospheric pollutants. These factors present deleterious effects on the cutaneous compartment by altering the skin barrier functions and accelerating the aging of the skin. Objectives: The goal of this study was to investigate the activity of Seaberry and Water Lily extracts, here called Dermina complex, against different external stresses. Methods: Human skin explants were exposed to different stimuli including delipidation by organic solvents, blue light, atmospheric pollutants and UV-A. The activity of the Seaberry and Water Lily extracts was assessed by immunohistochemistry and by biochemical assays. Results: We showed that Dermina complex prevents the delipidation-induced filaggrin decrease, suggesting that these plant extracts exhibited barrier function protecting properties. Also, we observed that Dermina complex showed an antioxidant and DNA protection activity by decreasing the activated form of Nrf2, the oxidized proteins and the formation of γ-H2AX induced upon stress conditions. The Dermina complex also decreased the pro-inflammatory cytokine IL-1 alpha released in the culture medium following atmospheric pollutants and UV-A exposure confirming its anti-inflammatory activity. Moreover, Dermina complex reduced the blue light-induced overexpression of opsin 3, indicating that its skin protection activities may be due, in part, to filter property against environmental stresses. Conclusions: Dermina complex shows a protective activity of the skin against different environmental stresses and these extracts may be able to slow down the aging process of the cutaneous compartment. 展开更多
关键词 Exposome Environmental Pollution Blue Light Oxidative Stress Plant EXTRACTS Skin EXPLANTS
在线阅读 免费下载
上一页 1 2 250 下一页 到第
使用帮助 返回顶部 意见反馈