There are abundant reports on the use of aromatic amino acids and creatinineas biomarkers in serum and urine forCKD(chronic kidney disease). However, investigationsinto these bio-species in bio-fluids, such as saliva ...There are abundant reports on the use of aromatic amino acids and creatinineas biomarkers in serum and urine forCKD(chronic kidney disease). However, investigationsinto these bio-species in bio-fluids, such as saliva and sweat, are rarely reported. Increasing interest in non-invasive methods for medical diagnosis advocates for the testing of these bio-fluidstoidentify potential biomarkers for prompt clinical andpreliminary screening using advanced analytical equipment. Unstimulated whole saliva samples were obtained from twenty-seven CKD patients and an equivalent number of healthy individuals. Saliva was assayed with ultra-performance liquid chromatography coupled with electro-spray ionization tandem mass spectrometry (UPLC-ESI-MS) in hydrophilic interaction chromatography mode. The data were analyzed using a student’s t test and receiver operatingcharacteristic (ROC) to evaluate the predictive power of several potential biomarkers (P<0.01) in saliva for preliminary CKD screening. Through testing of salivary samples between CKD patients and healthy individuals, we found three possible salivary biomarkers that demonstrated significant differences (P<0.01) from the nine reported species in serum and/or urine. The area under the curve (AUC) values for control vs CKDpatientsfor on L-phenylalanine, L- tryptophan, and creatinine were 0.863, 0.834, and 0.916, respectively. This is the first report to compare serum and urine biomarkers in saliva between CKD patients and healthy people. This study explores the potential of CKD diagnosis by saliva, and demonstrates a positive correlation between salivary and serum creatinine.展开更多
I.Manuscript Format ● Manuscripts should be in MS Word format. Authors are advised to download the document, Manuscript Submission Template, as a template or for more details on preparing their submissions for consid...I.Manuscript Format ● Manuscripts should be in MS Word format. Authors are advised to download the document, Manuscript Submission Template, as a template or for more details on preparing their submissions for consideration.展开更多
Using 116 earthquakes over ML3. 8 in the Inner Mongolia region from 2008 to 2015, the local earthquake magnitude ML and surface wave magnitude MS are remeasured. Based on norm linear regression (SR1 and SR2) and norm ...Using 116 earthquakes over ML3. 8 in the Inner Mongolia region from 2008 to 2015, the local earthquake magnitude ML and surface wave magnitude MS are remeasured. Based on norm linear regression (SR1 and SR2) and norm (OR) orthogonal regression method, we established the conversion relationship between ML and MS . The results were tested with Gaussian disturbance. The result shows that the orthogonal regression method (OR) result has the best fitting curve, and the conversion relation is MS = 0. 96 ML - 0. 10. The difference between our result and Guo Lücan's (MS = 1. 13 ML -1. 08) may be caused by regional tectonic characteristics. MS Inner Mongolia value is significantly higher than the MS empirical value, with an average difference of 0. 23, the difference distribution of empirical relation and the rectified relation is in the range of 0. 2-0. 3.展开更多
To reveal the geometry of the seismogenic structure of the Aug.8,2017 MS 7.0 Jiuzhaigou earthquake in northern Sichuan,data from the regional seismic network from the time of the main event to Oct.31,2017 were used to...To reveal the geometry of the seismogenic structure of the Aug.8,2017 MS 7.0 Jiuzhaigou earthquake in northern Sichuan,data from the regional seismic network from the time of the main event to Oct.31,2017 were used to relocate the earthquake sequence by the tomoDD program,and the focal mechanism solutions and centroid depths of the ML≥3.5 events in the sequence were determined using the CAP waveform inversion method.Further,the segmental tectonic deformation characteristics of the seismogenic faults were analyzed preliminarily by using strain rosettes and areal strains(As).The results indicate:(1)The relocated MS 7.0 Jiuzhaigou earthquake sequence displays a narrow~38 km long NNW-SSE-trending zone between the NW-striking Tazang Fault and the nearly NSstriking Minjiang Fault,two branches of the East Kunlun Fault Zone.The spatial distribution of the sequence is narrow and deep for the southern segment,and relatively wide and shallow for the northern segment.The initial rupture depth of the mainshock is 12.5 km,the dominant depth range of the aftershock sequence is between 0 and 10 km with an average depth of 6.7 km.The mainshock epicenter is located in the middle of the aftershock region,showing a bilateral rupture behavior.The centroid depths of 32 ML≥3.5 events range from 3 to 12 km with a mean of about 7.3 km,consistent with the predominant focal depth of the whole sequence.(2)The geometric structure of the seismogenic fault on the southern section of the aftershock area(south of the mainshock)is relatively simple,with overall strike of~150°and dip angle~75°,but the dip angle and dip-orientation exhibit some variation along the segment.The seismogenic structure on the northern segment is more complicated;several faults,including the Minjiang Fault,may be responsible for the aftershock activities.The overall strike of this section is~159°and dip angle is~59°,illustrating a certain clockwise rotation and a smaller dip angle than the southern segment.The differences between the two segments demonstrate vari展开更多
Objectives To establish a rapid analytical procedure for salmonella in textiles by LAMP in combination with MALDI TOF MS. The gene sequence invA of salmonella is specific to pathogenic strains. Based on its characteri...Objectives To establish a rapid analytical procedure for salmonella in textiles by LAMP in combination with MALDI TOF MS. The gene sequence invA of salmonella is specific to pathogenic strains. Based on its characteristic,a set of LAMP primer for salmonella is designed. If the amplification result is suspicious or positive,the strains are separated and the determination is rapidly performed through MALDI TOF MS technique. Methods About 70 reference strains closely related to the textiles and public health are selected to perform the specificity study(Salmonella enterica,Salmonella tyhimurium,Salmonella choleraesuis,Salmonella paratyphi A etc.);The bacterium solution of salmonella is diluted to different gradients for the sensitivity study. Results The results show this method is appropriately specific and sensitive,the limit of detection up to 4.6 CFU/mL. Conclusions The procedure is a new technique to detect salmonella in textiles.展开更多
文摘There are abundant reports on the use of aromatic amino acids and creatinineas biomarkers in serum and urine forCKD(chronic kidney disease). However, investigationsinto these bio-species in bio-fluids, such as saliva and sweat, are rarely reported. Increasing interest in non-invasive methods for medical diagnosis advocates for the testing of these bio-fluidstoidentify potential biomarkers for prompt clinical andpreliminary screening using advanced analytical equipment. Unstimulated whole saliva samples were obtained from twenty-seven CKD patients and an equivalent number of healthy individuals. Saliva was assayed with ultra-performance liquid chromatography coupled with electro-spray ionization tandem mass spectrometry (UPLC-ESI-MS) in hydrophilic interaction chromatography mode. The data were analyzed using a student’s t test and receiver operatingcharacteristic (ROC) to evaluate the predictive power of several potential biomarkers (P<0.01) in saliva for preliminary CKD screening. Through testing of salivary samples between CKD patients and healthy individuals, we found three possible salivary biomarkers that demonstrated significant differences (P<0.01) from the nine reported species in serum and/or urine. The area under the curve (AUC) values for control vs CKDpatientsfor on L-phenylalanine, L- tryptophan, and creatinine were 0.863, 0.834, and 0.916, respectively. This is the first report to compare serum and urine biomarkers in saliva between CKD patients and healthy people. This study explores the potential of CKD diagnosis by saliva, and demonstrates a positive correlation between salivary and serum creatinine.
文摘I.Manuscript Format ● Manuscripts should be in MS Word format. Authors are advised to download the document, Manuscript Submission Template, as a template or for more details on preparing their submissions for consideration.
基金Science for the Earthquake Resilience,China Earthquake Administration(XH18012)the Major Science and Technology Projects “Application Demonstration Research of Key Engineering Monitoring System Based on Microseismic Location Technology”,Inner Mongolia Autonomous Region.
文摘Using 116 earthquakes over ML3. 8 in the Inner Mongolia region from 2008 to 2015, the local earthquake magnitude ML and surface wave magnitude MS are remeasured. Based on norm linear regression (SR1 and SR2) and norm (OR) orthogonal regression method, we established the conversion relationship between ML and MS . The results were tested with Gaussian disturbance. The result shows that the orthogonal regression method (OR) result has the best fitting curve, and the conversion relation is MS = 0. 96 ML - 0. 10. The difference between our result and Guo Lücan's (MS = 1. 13 ML -1. 08) may be caused by regional tectonic characteristics. MS Inner Mongolia value is significantly higher than the MS empirical value, with an average difference of 0. 23, the difference distribution of empirical relation and the rectified relation is in the range of 0. 2-0. 3.
基金National Science Foundation of China(41574047)National Key R&D Program of China(2018YFC150330501).
文摘To reveal the geometry of the seismogenic structure of the Aug.8,2017 MS 7.0 Jiuzhaigou earthquake in northern Sichuan,data from the regional seismic network from the time of the main event to Oct.31,2017 were used to relocate the earthquake sequence by the tomoDD program,and the focal mechanism solutions and centroid depths of the ML≥3.5 events in the sequence were determined using the CAP waveform inversion method.Further,the segmental tectonic deformation characteristics of the seismogenic faults were analyzed preliminarily by using strain rosettes and areal strains(As).The results indicate:(1)The relocated MS 7.0 Jiuzhaigou earthquake sequence displays a narrow~38 km long NNW-SSE-trending zone between the NW-striking Tazang Fault and the nearly NSstriking Minjiang Fault,two branches of the East Kunlun Fault Zone.The spatial distribution of the sequence is narrow and deep for the southern segment,and relatively wide and shallow for the northern segment.The initial rupture depth of the mainshock is 12.5 km,the dominant depth range of the aftershock sequence is between 0 and 10 km with an average depth of 6.7 km.The mainshock epicenter is located in the middle of the aftershock region,showing a bilateral rupture behavior.The centroid depths of 32 ML≥3.5 events range from 3 to 12 km with a mean of about 7.3 km,consistent with the predominant focal depth of the whole sequence.(2)The geometric structure of the seismogenic fault on the southern section of the aftershock area(south of the mainshock)is relatively simple,with overall strike of~150°and dip angle~75°,but the dip angle and dip-orientation exhibit some variation along the segment.The seismogenic structure on the northern segment is more complicated;several faults,including the Minjiang Fault,may be responsible for the aftershock activities.The overall strike of this section is~159°and dip angle is~59°,illustrating a certain clockwise rotation and a smaller dip angle than the southern segment.The differences between the two segments demonstrate vari
文摘Objectives To establish a rapid analytical procedure for salmonella in textiles by LAMP in combination with MALDI TOF MS. The gene sequence invA of salmonella is specific to pathogenic strains. Based on its characteristic,a set of LAMP primer for salmonella is designed. If the amplification result is suspicious or positive,the strains are separated and the determination is rapidly performed through MALDI TOF MS technique. Methods About 70 reference strains closely related to the textiles and public health are selected to perform the specificity study(Salmonella enterica,Salmonella tyhimurium,Salmonella choleraesuis,Salmonella paratyphi A etc.);The bacterium solution of salmonella is diluted to different gradients for the sensitivity study. Results The results show this method is appropriately specific and sensitive,the limit of detection up to 4.6 CFU/mL. Conclusions The procedure is a new technique to detect salmonella in textiles.