期刊文献+
共找到10,928篇文章
< 1 2 250 >
每页显示 20 50 100
Different protein expression patterns in rat spinal nerves during wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling 预览
1
作者 Shuai Wei Xue-Zhen Liang +12 位作者 Qian Hu Wei-Shan Wang Wen-Jing Xu Xiao-Qing Cheng Jiang Peng Quan-Yi Guo Shu-Yun Liu Wen Jiang Xiao Ding Gong-Hai Han Ping Liu Chen-Hui Shi Yu Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期315-323,共9页
Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a label... Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the differences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between s 展开更多
关键词 gene ontology Kyoto ENCYCLOPEDIA of Genes and Genomes ISOBARIC tags for RELATIVE and absolute quantitation motor NERVE PROTEOMICS sensory NERVE spinal NERVE Wallerian degeneration
在线阅读 下载PDF
Protein microarray analysis of cytokine expression changes in distal stumps after sciatic nerve transection 预览
2
作者 Xiao-Qing Cheng Xue-Zhen Liang +9 位作者 Shuai Wei Xiao Ding Gong-Hai Han Ping Liu Xun Sun Qi Quan He Tang Qing Zhao Ai-Jia Shang Jiang Peng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期503-511,共9页
A large number of chemokines,cytokines,other trophic factors and the extracellular matrix molecules form a favorable microenvironment for peripheral nerve regeneration.This microenvironment is one of the major factors... A large number of chemokines,cytokines,other trophic factors and the extracellular matrix molecules form a favorable microenvironment for peripheral nerve regeneration.This microenvironment is one of the major factors for regenerative success.Therefore,it is important to investigate the key molecules and regulators affecting nerve regeneration after peripheral nerve injury.However,the identities of specific cytokines at various time points after sciatic nerve injury have not been determined.The study was performed by transecting the sciatic nerve to establish a model of peripheral nerve injury and to analyze,by protein microarray,the expression of different cytokines in the distal nerve after injury.Results showed a large number of cytokines were up-regulated at different time points post injury and several cytokines,e.g.,ciliary neurotrophic factor,were downregulated.The construction of a protein-protein interaction network was used to screen how the proteins interacted with differentially expressed cytokines.Kyoto Encyclopedia of Genes and Genomes pathway and Gene ontology analyses indicated that the differentially expressed cytokines were significantly associated with chemokine signaling pathways,Janus kinase/signal transducers and activators of transcription,phosphoinositide 3-kinase/protein kinase B,and notch signaling pathway.The cytokines involved in inflammation,immune response and cell chemotaxis were up-regulated initially and the cytokines involved in neuronal apoptotic processes,cell-cell adhesion,and cell proliferation were up-regulated at 28 days after injury.Western blot analysis showed that the expression and changes of hepatocyte growth factor,glial cell line-derived neurotrophic factor and ciliary neurotrophic factor were consistent with the results of protein microarray analysis.The results provide a comprehensive understanding of changes in cytokine expression and changes in these cytokines and classical signaling pathways and biological functions during Wallerian degeneration,as well as a bas 展开更多
关键词 cytokines DISTAL stump gene ontology KYOTO ENCYCLOPEDIA of Genes and Genomes pathway peripheral nerve injury protein microarray PROTEIN-PROTEIN interaction network Wallerian degeneration
在线阅读 下载PDF
Bioinformatic identification of key candidate genes and pathways in axon regeneration after spinal cord injury in zebrafish 预览
3
作者 Jia-He Li Zhong-Ju Shi +6 位作者 Yan Li Bin Pan Shi-Yang Yuan Lin-Lin Shi Yan Hao Fu-Jiang Cao Shi-Qing Feng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第1期103-111,共9页
Zebrafish and human genomes are highly homologous;however,despite this genomic similarity,adult zebrafish can achieve neuronal proliferation,regeneration and functional restoration within 6–8 weeks after spinal cord ... Zebrafish and human genomes are highly homologous;however,despite this genomic similarity,adult zebrafish can achieve neuronal proliferation,regeneration and functional restoration within 6–8 weeks after spinal cord injury,whereas humans cannot.To analyze differentially expressed zebrafish genes between axon-regenerated neurons and axon-non-regenerated neurons after spinal cord injury,and to explore the key genes and pathways of axonal regeneration after spinal cord injury,microarray GSE56842 was analyzed using the online tool,GEO2R,in the Gene Expression Omnibus database.Gene ontology and protein-protein interaction networks were used to analyze the identified differentially expressed genes.Finally,we screened for genes and pathways that may play a role in spinal cord injury repair in zebrafish and mammals.A total of 636 differentially expressed genes were obtained,including 255 up-regulated and 381 down-regulated differentially expressed genes in axon-regenerated neurons.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment results were also obtained.A protein-protein interaction network contained 480 node genes and 1976 node connections.We also obtained the 10 hub genes with the highest correlation and the two modules with the highest score.The results showed that spectrin may promote axonal regeneration after spinal cord injury in zebrafish.Transforming growth factor beta signaling may inhibit repair after spinal cord injury in zebrafish.Focal adhesion or tight junctions may play an important role in the migration and proliferation of some cells,such as Schwann cells or neural progenitor cells,after spinal cord injury in zebrafish.Bioinformatic analysis identified key candidate genes and pathways in axonal regeneration after spinal cord injury in zebrafish,providing targets for treatment of spinal cord injury in mammals. 展开更多
关键词 axonal REGENERATION differentially expressed GENES focal ADHESIONS Gene Ontology Kyoto Encyclopedia of GENES and Genomes neural REGENERATION protein-protein interaction network SIGNALING PATHWAY SPECTRIN tight junctions transforming growth factor beta Wnt SIGNALING PATHWAY
在线阅读 下载PDF
TIR-Learner, a New Ensemble Method for TIR Transposable Element Annotation, Provides Evidenee for Abundant New Transposable Elements in the Maize Genome
4
作者 Weijia Su Xun Gu Thomas Peterson 《分子植物:英文版》 SCIE CAS CSCD 2019年第3期447-460,共14页
Transposable elements (TEs) make up a large and rapidly evolving proportion of plant genomes. Among Class II DNA TEs, TIR elements are flanked by characteristic terminal inverted repeat sequences (TIRs). TIR TEs may p... Transposable elements (TEs) make up a large and rapidly evolving proportion of plant genomes. Among Class II DNA TEs, TIR elements are flanked by characteristic terminal inverted repeat sequences (TIRs). TIR TEs may play important roles in genome evolution, including generating allelic diversity, inducing structural variation, and regulating gene expression. However, TIR TE identification and annotation has been hampered by the lack of effective tools, resulting in erroneous TE annotations and a significant underestimation of the proportion of TIR elements in the maize genome. This problem has largely limited our understanding of the impact of TIR elements on plant genome structure and evolution. In this paper, we propose a new method of TIR element detection and annotation. This new pipeline combines the advantages of current homology-based annotation methods with powerful de novo machine-learning approaches, resulting in greatly increased efficiency and accuracy of TIR element annotation. The results show that the copy number and genome proportion of TIR elements in maize is much larger than that of current annotations. In addition, the distribution of some TIR superfamily elements is reduced in centromeric and pericentromeric positions, while others do not show a similar bias. Finally, the incorporation of machine-learning techniques has enabled the identification of large numbers of new DTA (hAT) family elements, which have all the hallmarks of bona fide TEs yet which lack high homology with currently known DTA elements. Together, these results provide new tools forTE research and new insight into the impact ofTIR elements on maize genome diversity . 展开更多
关键词 maize GENOMES TIR TRANSPOSABLE element ANNOTATION pipeline machine learning
SeqSQC:A Bioconductor Package for Evaluating the Sample Quality of Next-generation Sequencing Data
5
作者 Qian Liu Qiang Hu +7 位作者 Song Yao Marilyn L. Kwan Janise M. Roh Hua Zhao Christine B. Ambrosone Lawrence H. Kushi Song Liu Qianqian Zhu 《基因组蛋白质组与生物信息学报:英文版》 CAS CSCD 2019年第2期211-218,共8页
As next-generation sequencing (NGS) technology has become widely used to identify genetic causal variants for various diseases and traits,a number of packages for checking NGS data quality have sprung up in public dom... As next-generation sequencing (NGS) technology has become widely used to identify genetic causal variants for various diseases and traits,a number of packages for checking NGS data quality have sprung up in public domains. In addition to the quality of sequencing data,sample quality issues,such as gender mismatch,abnormal inbreeding coefficient,cryptic relatedness,and population outliers,can also have fundamental impact on downstream analysis. However,there is a lack of tools specialized in identifying problematic samples from NGS data,often due to the limitation of sample size and variant counts. We developed SeqSQC,a Bioconductor package,to automate and accelerate sample cleaning in NGS data of any scale. SeqSQC is designed for efficient data storage and access,and equipped with interactive plots for intuitive data visualization to expedite the identification of problematic samples. SeqSQC is available at http://bioconductor. org/packages/SeqSQC. 展开更多
关键词 Next-generation SEQUENCING QUALITY assessment 1000 GENOMES Project Whole-exome SEQUENCING BIOCONDUCTOR PACKAGE
MapMan4: A Refined Protein Classification and Annotation Framework Applicable to Multi-Omics Data Analysis
6
作者 Rainer Schwacke Gabriel Y.Ponce-Soto +7 位作者 Kirsten Krause Anthony M.Bolger Borjana Arsova Asis Hallab Kristina Gruden Mark Stitt Marie E.Bolger Bjorn Usadel 《分子植物:英文版》 SCIE CAS CSCD 2019年第6期879-892,共14页
Genome sequences from over 200 plant species have already been published, with this number expected to increase rapidly due to advances in sequencing technologies. Once a new genome has been assembled and the genes id... Genome sequences from over 200 plant species have already been published, with this number expected to increase rapidly due to advances in sequencing technologies. Once a new genome has been assembled and the genes identified, the functional annotation of their putative translational products, proteins, using ontologies is of key importance as it places the sequencing data in a biological context. Furthermore, to keep pace with rapid production of genome sequences, this functional annotation process must be fully automated. Here we present a redesigned and significantly enhanced MapMan4 framework, together with a revised version of the associated online Mercator annotation tool. Compared with the original MapMan, the new ontology has been expanded almost threefold and enforces stricter assignment rules. This framework was then incorporated into Mercator4, which has been upgraded to reflect current knowledge across the land plant group, providing protein annotations for all embryophytes with a comparably high quality. The annotation process has been optimized to allow a plant genome to be annotated in a matter of minutes. The output results continue to be compatible with the established MapMan desktop application. 展开更多
关键词 Plant GENOMES Functional ANNOTATION MapMan Mercator TRANSCRIPTOMES
Investigating loss of heterozygosity in a SCRaMbLEd yeast genome
7
作者 Junbiao Dai 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第6期868-869,共2页
Loss of heterozygosity (LOH) is a phenomenon in which elimination of one parental genomic region occurs in interspecific hybrids. LOH is common in cancer (Deng et al., 1996;Koufos et al., 1985). However, genetic analy... Loss of heterozygosity (LOH) is a phenomenon in which elimination of one parental genomic region occurs in interspecific hybrids. LOH is common in cancer (Deng et al., 1996;Koufos et al., 1985). However, genetic analysis of LOH has been mainly conducted based on predetermined features of individual genomes. 展开更多
关键词 LOSS of HETEROZYGOSITY ELIMINATION INDIVIDUAL GENOMES
Bioinformatics analyses of differentially expressed genes associated with spinal cord injury:a microarray-based analysis in a mouse model 预览
8
作者 Lei Guo Jing Lv +2 位作者 Yun-Fei Huang Ding-Jun Hao Ji-Jun Liu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1262-1270,共9页
Gene spectrum analysis has shown that gene expression and signaling pathways change dramatically after spinal cord injury,which may affect the microenvironment of the damaged site.Microarray analysis provides a new op... Gene spectrum analysis has shown that gene expression and signaling pathways change dramatically after spinal cord injury,which may affect the microenvironment of the damaged site.Microarray analysis provides a new opportunity for investigating diagnosis,treatment,and prognosis of spinal cord injury.However,differentially expressed genes are not consistent among studies,and many key genes and signaling pathways have not yet been accurately studied.GSE5296 was retrieved from the Gene Expression Omnibus DataSet.Differentially expressed genes were obtained using R/Bioconductor software(expression changed at least two-fold;P<0.05).Database for Annotation,Visualization and Integrated Discovery was used for functional annotation of differentially expressed genes and Animal Transcription Factor Database for predicting potential transcription factors.The resulting transcription regulatory protein interaction network was mapped to screen representative genes and investigate their diagnostic and therapeutic value for disease.In total,this study identified 109 genes that were upregulated and 30 that were downregulated at 0.5,4,and 24 hours,and 3,7,and 28 days after spinal cord injury.The number of downregulated genes was smaller than the number of upregulated genes at each time point.Database for Annotation,Visualization and Integrated Discovery analysis found that many inflammation-related pathways were upregulated in injured spinal cord.Additionally,expression levels of these inflammation-related genes were maintained for at least 28 days.Moreover,399 regulation modes and 77 nodes were shown in the protein-protein interaction network of upregulated differentially expressed genes.Among the 10 upregulated differentially expressed genes with the highest degrees of distribution,six genes were transcription factors.Among these transcription factors,ATF3 showed the greatest change.ATF3 was upregulated within 30 minutes,and its expression levels remained high at 28 days after spinal cord injury.These key genes screened by bioinf 展开更多
关键词 nerve REGENERATION spinal cord injury differentially expressed GENES BIOINFORMATICS ANALYSES Database for Annotation Visualization and Integrated Discovery ANALYSIS inflammation Kyoto Encyclopedia of GENES and Genomes pathway MICROARRAY transcription factors neural REGENERATION
在线阅读 下载PDF
拟穴青蟹两个Crustin新变体的克隆与表达分析 预览
9
作者 张财亮 黄贝 +2 位作者 熊静 陈芳奕 黄文树 《厦门大学学报:自然科学版》 CAS CSCD 北大核心 2019年第4期505-515,共11页
Crustins是一类广泛存在于甲壳动物中富含半胱氨酸的阳离子抗菌肽.该研究从拟穴青蟹(Scylla Paramamosain)中克隆获得两个Crustin新变体,分别命名为SpCrus1b和SpCrus2b.分析显示两者的前体肽包含N-端信号肽和C-端成熟肽两部分,成熟肽均... Crustins是一类广泛存在于甲壳动物中富含半胱氨酸的阳离子抗菌肽.该研究从拟穴青蟹(Scylla Paramamosain)中克隆获得两个Crustin新变体,分别命名为SpCrus1b和SpCrus2b.分析显示两者的前体肽包含N-端信号肽和C-端成熟肽两部分,成熟肽均含有12个位置高度保守的半胱氨酸.SpCrus1b在信号肽和乳清酸蛋白(WAP)结构域之间存在一个半胱氨酸富集区,属于典型的Ⅰ型Crustins;SpCrus2b除具有SpCrus1b的结构特征外,在近N-端额外含有一个甘氨酸富集区,属于典型的Ⅱ型Crustins.基因组结构分析显示SpCrus1b的基因组DNA序列为4个外显子/3个内含子结构,而SpCrus2b的基因组DNA序列为2个外显子/1个内含子结构.两者均主要在鳃中表达,且在脂多糖(LPS)刺激24h和聚肌胞苷酸(PolyI:C)刺激12h后表达量均极显著上调(p<0.01),表明SpCrus1b和SpCrus2b参与了拟穴青蟹抗细菌和抗病毒的先天免疫过程. 展开更多
关键词 抗菌肽 CRUSTIN 基因克隆 基因组 表达分析 拟穴青蟹$
在线阅读 免费下载
Opportunities to Use DNA Methylation to Distil Functional Elements in Large Crop Genomes
10
作者 Peter A.Crisp Jaclyn M.Noshay +1 位作者 Sarah N.Anderson Nathan M.Springer 《分子植物:英文版》 SCIE CAS CSCD 2019年第3期282-284,共3页
DNA methylation is a chromatin modification that is often associated with the exciti ng and sometimes unpredictable patter ns of inheritanee that can unfold with epigenetic phenomena. The stability and heritability of... DNA methylation is a chromatin modification that is often associated with the exciti ng and sometimes unpredictable patter ns of inheritanee that can unfold with epigenetic phenomena. The stability and heritability of DNA methylati on patter ns perhaps allow us to utilize DNA methylation profiles to distil the suite of potentially functional elements in large crop genomes. Here, we discuss the potential and possible ways to use the absence of DNA methylation to identify potential regulatory regions within intergenic sequences and the presence of DNA methylation to identify pseudogenes. 展开更多
关键词 DNA METHYLATION LARGE CROP GENOMES
Complete loss of RNA editing from the plastid genome and most highly expressed mitochondrial genes of Welwitschia mirabilis
11
作者 Weishu Fan Wenhu Guo +2 位作者 Lexis Funk Jeffrey P.Mower Andan Zhu 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第4期498-506,共9页
Comparative genomics among gymnosperms suggested extensive loss of mitochondrial RNA editing sites from Welwitschia mirabilis based on predictive analysis.However,empirical or transcriptome data to confirm this massiv... Comparative genomics among gymnosperms suggested extensive loss of mitochondrial RNA editing sites from Welwitschia mirabilis based on predictive analysis.However,empirical or transcriptome data to confirm this massive loss event are lacking,and the potential mechanisms of RNA site loss are unclear.By comparing genomic sequences with transcriptomic and reversetranscription PCR sequencing data,we performed a comprehensive analysis of the pattern of RNA editing in the mitochondrial and plastid genomes(mitogenome and plastome,respectively)of W.mirabilis and a second gymnosperm,Ginkgo biloba.For W.mirabilis,we found only 99 editing sites located in 13 protein-coding genes in the mitogenome and a complete loss of RNA editing from the plastome.The few genes having high editing frequency in the Welwitschia mitogenome showed a strong negative correlation with gene expression level.Comparative analyses with G.biloba,containing 1,405 mitochondrial and 345 plastid editing sites,revealed that the editing loss from W.mirabilis is mainly due to the substitution of editable cytidines to thymidines at the genomic level,which could be caused by retroprocessing.Our result is the first study to uncover massive editing loss from both the mitogenome and plastome in a single genus.Furthermore,our results suggest that gene expression level and retroprocessing both contributed to the evolution of RNA editing in plant organellar genomes. 展开更多
关键词 RNA EDITING MASSIVE LOSS expression levels ORGANELLE GENOMES Welwitschia
Analysis of the autophagy gene expression profile of pancreatic cancer based on autophagy-related protein microtubule-associated protein 1A/1B-light chain 3 预览
12
作者 Yan-Hui Yang Yu-Xiang Zhang +3 位作者 Yang Gui Jiang-Bo Liu Jun-Jun Sun Hua Fan 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第17期2086-2098,共13页
BACKGROUND Pancreatic cancer is a highly invasive malignant tumor. Expression levels of the autophagy-related protein microtubule-associated protein 1A/1B-light chain 3 (LC3) and perineural invasion (PNI) are closely ... BACKGROUND Pancreatic cancer is a highly invasive malignant tumor. Expression levels of the autophagy-related protein microtubule-associated protein 1A/1B-light chain 3 (LC3) and perineural invasion (PNI) are closely related to its occurrence and development. Our previous results showed that the high expression of LC3 was positively correlated with PNI in the patients with pancreatic cancer. In this study, we further searched for differential genes involved in autophagy of pancreatic cancer by gene expression profiling and analyzed their biological functions in pancreatic cancer, which provides a theoretical basis for elucidating the pathophysiological mechanism of autophagy in pancreatic cancer and PNI. AIM To identify differentially expressed genes involved in pancreatic cancer autophagy and explore the pathogenesis at the molecular level. METHODS Two sets of gene expression profiles of pancreatic cancer/normal tissue (GSE16515 and GSE15471) were collected from the Gene Expression Omnibus. Significance analysis of microarrays algorithm was used to screen differentially expressed genes related to pancreatic cancer. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to analyze the functional enrichment of the differentially expressed genes. Protein interaction data containing only differentially expressed genes was downloaded from String database and screened. Module mining was carried out by Cytoscape software and ClusterOne plug-in. The interaction relationship between the modules was analyzed and the pivot nodes between the functional modules were determined according to the information of the functional modules and the data of reliable protein interaction network.RESULTS Based on the above two data sets of pancreatic tissue total gene expression, 6098 and 12928 differentially expressed genes were obtained by analysis of genes with higher phenotypic correlation. After extracting the intersection of the two differential gene sets, 4870 genes were determined 展开更多
关键词 Pancreatic cancer Autophagy-related PROTEIN microtubule-associated PROTEIN 1A/1B-light chain 3 Perineural invasion Gene Ontology ANALYSIS KYOTO ENCYCLOPEDIA of Genes and Genomes pathway ANALYSIS Ubiquitin C
在线阅读 免费下载
基于高通量测序的虾夷扇贝基因组微卫星特征分析 预览 被引量:2
13
作者 倪守胜 杨钰 +1 位作者 柳淑芳 庄志猛 《渔业科学进展》 CSCD 北大核心 2018年第1期107-113,共7页
为全面了解虾夷扇贝(Patinopecten yessoensis)微卫星分布频率和数量,深化对虾夷扇贝基因组的认识,本研究运用第二代高通量测序技术,进行虾夷扇贝简化基因组测序(RAD-seq),从基因组水平阐明虾夷扇贝基因组微卫星特征。结果显示,简化基... 为全面了解虾夷扇贝(Patinopecten yessoensis)微卫星分布频率和数量,深化对虾夷扇贝基因组的认识,本研究运用第二代高通量测序技术,进行虾夷扇贝简化基因组测序(RAD-seq),从基因组水平阐明虾夷扇贝基因组微卫星特征。结果显示,简化基因组测序共获得序列总长为92,551,435 bp,经过滤筛选,获得259,535个contig,其中,包含微卫星序列3618条,经引物设计共获得3460对微卫星引物。统计微卫星序列的重复类型,其中,三核苷酸重复单元数量最多(1587个,45.87%),其次是二核苷酸重复(1282个,37.05%),六核苷酸重复单元数量最少(20个,0.58%)。在三核苷酸重复中,以ATA重复类型所占比例最高(11.41%),共计181个。此外,虾夷扇贝同一重复类型的微卫星随着重复数增加其数量相对减少,而相同重复数的微卫星随着重复单元长度的增加其数量也呈下降趋势,可见微卫星长度与其数量呈负相关,表明长度较短的微卫星变异速率较快。本研究结果为认识虾夷扇贝基因组特征和在基因组水平开展种群遗传学研究提供了基础数据。 展开更多
关键词 虾夷扇贝 二代测序 基因组 微卫星
在线阅读 免费下载
木荷基因组SSR位点开发及初步分析 预览
14
作者 林艳 何紫迪 +3 位作者 毛积鹏 蒋开彬 王金榜 黄少伟 《热带作物学报》 CSCD 北大核心 2018年第9期1766-1771,共6页
为开发木荷分子标记,采用高通量测序技术获得木荷基因组原始数据,经生物信息学软件对木荷基因组序列进行序列拼接、组装和对比,共获得308 418条Contig序列和459 984条Scafford序列。采用MISA软件搜索木荷基因组序列中微卫星(Microsatel... 为开发木荷分子标记,采用高通量测序技术获得木荷基因组原始数据,经生物信息学软件对木荷基因组序列进行序列拼接、组装和对比,共获得308 418条Contig序列和459 984条Scafford序列。采用MISA软件搜索木荷基因组序列中微卫星(Microsatellite)位点,共得到334 843个SSR序列,总长度5 074 708 bp,占木荷基因组大小的0.98%,木荷基因组SSR序列平均长度为15.2 bp,平均分布频率为644个/Mb。木荷SSR序列中,单核苷酸序列数量最多,共188 217个,占木荷SSR序列总数的56.21%,其次是二核苷酸(23%)〉三核苷酸(13%)〉四核苷酸(5%)〉五核苷酸(2%)〉六核苷酸(1%)。木荷全基因组SSR序列中共包括400种重复基元,其中单核苷酸重复基元A和二核苷酸重复基元AT是主要重复基元,分别占总SSR的56%和11%,SSR基元的重复次数分布在4~40次,主要分布在4~25次。本研究丰富了木荷分子标记类型,为进一步群体遗传结构和遗传多样性分析提供了基础数据。 展开更多
关键词 木荷 SSR标记 生物信息学 基因组 高通量
在线阅读 下载PDF
Reconstructing evolutionary timescales using phylogenomics
15
作者 K. Jun Tong Nathan Lo Simon Y. W. Ho 《动物分类学报:英文版》 CSCD 2016年第4期343-351,共9页
Reconstructing the timescale of the Tree of Life is one of the principal aims of evolutionary biology.This has been greatly aided by the development of the molecular clock,which enables evolutionary timescales to be e... Reconstructing the timescale of the Tree of Life is one of the principal aims of evolutionary biology.This has been greatly aided by the development of the molecular clock,which enables evolutionary timescales to be estimated from genetic data.In recent years,high-throughput sequencing technology has led to an increase in the feasibility and availability of genome-scale data sets.These represent a rich source of biological information,but they also bring a set of analytical challenges.In this review,we provide an overview of phylogenomic dating and describe the challenges associated with analysing genome-scale data.We also report on recent phylogenomic estimates of the evolutionary timescales of mammals,birds,and insects. 展开更多
长穗偃麦草优异基因的染色体定位及应用 被引量:2
16
作者 陈士强 何震天 +4 位作者 张容 王建华 王锦荣 陈建民 陈秀兰 《植物遗传资源学报》 CAS CSCD 北大核心 2015年第5期1062-1066,1072共6页
长穗偃麦草比较公认的有2个种,即二倍体长穗偃麦草(Thinopyrum elongatum,2n=2X)和十倍体长穗偃麦草(Thinopyrum ponticum,2n=10X),是重要的小麦近缘种,具有抗病、抗寒、抗旱、耐盐碱等优良性状。因其基因组中蕴含许多对小麦品种改... 长穗偃麦草比较公认的有2个种,即二倍体长穗偃麦草(Thinopyrum elongatum,2n=2X)和十倍体长穗偃麦草(Thinopyrum ponticum,2n=10X),是重要的小麦近缘种,具有抗病、抗寒、抗旱、耐盐碱等优良性状。因其基因组中蕴含许多对小麦品种改良极为有用的基因,且易与小麦杂交等优势,多年来长穗偃麦草一直作为小麦遗传改良的优良种质资源而备受关注。本文对长穗偃麦草的基因组研究及其在小麦的抗逆、抗病和提高光合能力、产量及高分子量谷蛋白(HMW-GS)含量等方面的应用做了综述,为其基因组中优异基因的进一步开发和利用提供了理论依据。 展开更多
关键词 长穗偃麦草 基因组 小麦 抗病
Helicobacter heilmannii sensu lato :An overview of the infection in humans 预览
17
作者 Mario Bento-Miranda Ceu Figueiredo 《世界胃肠病学杂志:英文版(电子版)》 SCIE CAS 2014年第47期17779-17787,共9页
Helicobacter heilmannii sensu lato(H.heilmannii s.l.)is a group of gastric non-Helicobacter pylori Helicobacter species that are morphologically indistinguishable from each other.H.heilmannii s.l.infect the stomach of... Helicobacter heilmannii sensu lato(H.heilmannii s.l.)is a group of gastric non-Helicobacter pylori Helicobacter species that are morphologically indistinguishable from each other.H.heilmannii s.l.infect the stomach of several animals and may have zoonotic potential.Although the prevalence of these infections in humans is low,they are associated with gastric pathology,including mucosa-associated lymphoid tissue lymphoma,making them a significant health issue.Here,the taxonomy,epidemiology,microbiology,diagnosis,and treatment of these infections will be reviewed.The gastric pathology associated with H.heilmannii s.l.infections in humans will also be addressed.Finally,the features of the complete bacterial genomes available and studies on species-specific pathogenesis will be reviewed.The understanding of the mechanisms that underlie gastric disease development mediated by the different bacterial species that constitute H.heilmannii s.l.is essential for developing strategies for prevention and treatment of these infections. 展开更多
关键词 HELICOBACTER heilmannii sensu lato GASTRIC non-Hel
在线阅读 免费下载
核糖核酸酶基因超家族分子进化 预览 被引量:5
18
作者 郎大田 张亚平 于黎 《遗传》 CAS CSCD 北大核心 2014年第4期316-326,共11页
核糖核酸酶基因(Ribonuclease A,RNASE A)超家族是进化生物学中研究新基因起源及新功能演变的重要模式系统之一.RNASEA超家族中的很多成员表现出基因复制的进化模式,而且在适应性(正)选择的驱动下,发生了功能分化.文章综述了RNASE ... 核糖核酸酶基因(Ribonuclease A,RNASE A)超家族是进化生物学中研究新基因起源及新功能演变的重要模式系统之一.RNASEA超家族中的很多成员表现出基因复制的进化模式,而且在适应性(正)选择的驱动下,发生了功能分化.文章综述了RNASE A超家族成员在不同动物类群中进化模式的研究进展,包括近年来越来越多在基因组水平上开展的相关研究,显示该基因超家族可能具有比人们以往认识的更为复杂的基因进化模式.随着越来越多动物基因组数据的产生,对更多动物代表类群进行RNASE A超家族研究,将有望揭示新的进化机制和功能分化,为系统认识动物适应进化的遗传机制奠定基础. 展开更多
关键词 核糖核酸酶基因超家族 适应性进化 基因复制 功能分化 基因组
在线阅读 下载PDF
滇杨优树基因组及其无性系落叶期侧芽cDNA的AFLP分析 预览 被引量:2
19
作者 李里 江涛 +4 位作者 王滨蔚 吴海 周安佩 刘东玉 何承忠 《分子植物育种》 CAS CSCD 北大核心 2013年第3期393-402,共10页
本研究采用AFLP分子标记技术,对采集于四川省和云南省的56株滇杨(Populus yunnanensisDode)优树基因组遗传变异、54个滇杨优树无性系3a苗木落叶期侧芽的基因表达谱进行了分析。DNA—AFLP分析结果表明,筛选出的8对AFLP引物组合共扩增... 本研究采用AFLP分子标记技术,对采集于四川省和云南省的56株滇杨(Populus yunnanensisDode)优树基因组遗传变异、54个滇杨优树无性系3a苗木落叶期侧芽的基因表达谱进行了分析。DNA—AFLP分析结果表明,筛选出的8对AFLP引物组合共扩增出508条带,平均多态性条带比率为59.25%,观测等位基因数为1.5925,有效等位基因数为1.1581,Nei’S基因多样性指数为0.1129,Shannon’S信息指数为0.1914;除有效等位基因数相等外,四川滇杨优树群体的4项遗传多样性指标均略高于云南滇杨优树群体,但2个优树群体之间存在着较高的基因流(Nm=21.64)。cDNA—AFLP分析结果显示,筛选出的8对AFLP引物组合共扩增出526条带,其中平均差异性条带百分率为82.70%,观测等位基因数和有效等位基因数分别为1.8270和1.2417,Nei’S基因多样性指数为0.1675,Shannon’S信息指数为0.2809;四川滇杨优树群体的基因表达差异性指标均略高于云南滇杨优树群体,且二者之间的基因流值较大(Nm=27.00)。分子方差分析(AMOVA)结果表明,滇杨2个优树群体在DNA水平和eDNA水平的遗传差异均极显著。 展开更多
关键词 滇杨优树 基因组 侧芽cDNA 落叶期 AFLP标记
在线阅读 下载PDF
新型布尼亚病毒安徽分离株全基因组测序分析 被引量:5
20
作者 孙永 张永根 +2 位作者 胡万富 何军 王俊 《安徽预防医学杂志》 2013年第6期403-405,共3页
目的对安徽省两株新型布尼亚病毒进行全基因组序列测定,分析其全基因组序列的基因特征与变异特点。方法应用22对测序引物对病毒基因组进行RT—PCR分段扩增,PCR产物直接进行序列测定,Clustal W、DNASTAR、MEGA4.1等软件分析基因组序... 目的对安徽省两株新型布尼亚病毒进行全基因组序列测定,分析其全基因组序列的基因特征与变异特点。方法应用22对测序引物对病毒基因组进行RT—PCR分段扩增,PCR产物直接进行序列测定,Clustal W、DNASTAR、MEGA4.1等软件分析基因组序列。结果两株病毒全基因组编码情况和已报道新型布尼亚病毒相同,未见插入和缺失;构建系统发生树可见AHZ毒株与河南省毒株进化距离最近,而AHL毒株在一支较为独立的分支上;M片段膜蛋白前体编码区氨基酸变异率最高。结论安徽省新型布尼亚病毒毒株基因组相对稳定,可能存在与河南省毒株相互传播的现象。 展开更多
关键词 发热伴血小板减少综合征 布尼亚病毒 基因组 序列分析
上一页 1 2 250 下一页 到第
使用帮助 返回顶部 意见反馈
新型冠状病毒肺炎防控与诊疗专栏