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Effects of Low Temperature and Low Light on Physiology of Tomato Seedlings 认领
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作者 Yubo Yang Lingdi Dong +5 位作者 Linqi Shi Jinghua Guo Yonggang Jiao Haizheng Xiong Ryan William Dickson Ainong Shi 《美国植物学期刊(英文)》 2020年第2期162-179,共18页
The physiological changes and the mechanism of stress tolerance in tomato were studied under low temperature and low light conditions. Two growth chamber experiments evaluated three temperatures regimes under standard... The physiological changes and the mechanism of stress tolerance in tomato were studied under low temperature and low light conditions. Two growth chamber experiments evaluated three temperatures regimes under standard and relatively low illumination levels with three tomato genotypes. Both experiments used a completely randomized split-plot design (CRD), with temperature regime as the main plot and tomato genotype as the split-plot. The three tomato varieties were “Fenyan No.1”, “SV0313TG”, and “Ousa”. In both experiments, activity of superoxide dismutase (SOD) and peroxidases (POD) in tomato seedlings decreased under low temperature regime and the combination of low temperature and low light. Decreasing temperature had the greatest effect on the increase in enzyme activity. Decrease in POD activity was the greatest under low light and low temperature. The concentration of malondialdehyde (MDA) in plant tissue also decreased under low temperature (20°C/10°C day/night) compared to the standard temperature control (25°C/16°C day/night), but increased at 15°C/5°C day/night temperatures in both experiments and was the greatest under the lowest light and temperature conditions. In both experiments, proline concentrations were the greatest under the standard light intensity (30,000 lux), and proline concentrations increased as temperature decreased. The content of soluble sugar decreased under only low temperature stress but increased under double stresses. The relative value of osmotic potential increased a little under low temperature stress but decreased under double stresses. 展开更多
关键词 Enzyme Activity MALONDIALDEHYDE OSMOTIC Potential PEROXIDASES PROLINE Physiological Condition Soluble Sugar Stress Tolerance Superoxide DISMUTASE
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Effect of Gubi prescription on caveolin-1 expression and phosphoinositide 3 kinase/protein kinase B and Fas signal pathways in rats with knee osteoarthritis 认领
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作者 He Xiaojin Wang Lei +5 位作者 Zhou Xueping Xu Luzhou Cao Jing Wang Ruirui Wang Min Xie Guoqian 《中医杂志:英文版》 SCIE CAS CSCD 2020年第2期224-235,共12页
OBJECTIVE: To investigate the effects of Gubi prescription on the expression of caveolin-1, and the phosphoinositide 3 kinase/protein kinase B(PI3 K/Akt) and Fas signal pathways in rats with knee osteoarthritis(KOA).M... OBJECTIVE: To investigate the effects of Gubi prescription on the expression of caveolin-1, and the phosphoinositide 3 kinase/protein kinase B(PI3 K/Akt) and Fas signal pathways in rats with knee osteoarthritis(KOA).METHODS: Forty KOA model rats were established using a modification of Hulth's method. Rats were divided into five groups by the random number method: model, positive drug(Vicolli group), and high-, medium-, and low-dose Gubi prescription groups(n = 8/group). In the sham surgery group(n = 8), only anterior and posterior cruciate ligaments of rats were exposed during surgery. A normal group(n = 8) consisted of rats with no treatment. Rats were intragastrically administered corresponding drugs once every day for eight consecutive weeks. Then, rat synovial membranes were extracted and histomorphological changes were recorded. m RNA expression was measured by q-PCR.Serum superoxide dismutase(SOD), malondialdehyde(MDA), nitric oxide(NO), and interleukin 1β(IL-1β) levels were measured. Western blotting determined the effects of Gubi prescription on protein expressions of caveolin-1, Bax, Bcl-2, Fas, and caspase-3 in chondrocytes from KOA rats. The knee cartilage of rats was excised and cultured under aseptic conditions. After coincubation of chondrocytes with Gubi prescription-containing serum, IL-1β, and si RNA, Western blotting was used to determine the protein expressions of caveolin-1, Bax, Bcl-2, Fas, and caspase-3.RESULTS: The morphological score of the articular synovium in the model group was significantly higher than in the normal group(P < 0.01). The morphological score in the high-and mediumdose Gubi prescription groups was lower than in the model group(P < 0.05). Chondrocytes from the decoction-containing serum group had a lower expression of Bax(P < 0.05), and higher expressions of Bcl-2(P < 0.05) and caspase-3(P < 0.05) compared with the model group. Chondrocytes in the decoction-containing serum group had higher expressions of Bax and Bcl-2(P < 0.01) and lower expressions of caveolin-1 and 展开更多
关键词 Osteoarthritis knee Caveolin 1 Phosphatidylinositol 3-kinase Superoxide dismutase MALONDIALDEHYDE Nitric oxide Interleukin-1beta Gubi prescription
Hydrogen promotes the activation of Cu, Zn superoxide dismutase in a rat corneal alkali-burn model 认领
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作者 Takeshi Arima Tsutomu Igarashi +4 位作者 Masaaki Uchiyama Maika Kobayashi Ikuroh Ohsawa Akira Shimizu Hiroshi Takahashi 《国际眼科杂志:英文版》 SCIE CAS 2020年第8期1173-1179,共7页
AIM: To investigate the effects of hydrogen(H2) on Cu, Zn superoxide dismutase(SOD1) activation in a rat model of corneal alkali burn. METHODS: In each rat, one cornea was subjected to alkali exposure. Physiological s... AIM: To investigate the effects of hydrogen(H2) on Cu, Zn superoxide dismutase(SOD1) activation in a rat model of corneal alkali burn. METHODS: In each rat, one cornea was subjected to alkali exposure. Physiological saline(saline group) or H2-dissolved saline(H2 group) was instilled continuously on the cornea for 5 min before and after alkali exposure. Inflammatory cells, neovascularization, and cytoplasmic SOD1 levels were evaluated immunohistochemically in enucleated eyes from both groups. Three-dimensional ultrastructural tissue changes in the eyes were analyzed using low-vacuum scanning electron microscopy.RESULTS: The numbers of both inflammatory and vascular endothelial cells were significantly reduced in the corneas of the H2 group(P<0.01). Furthermore, H2 treatment increased both cytoplasmic SOD1 levels(P<0.01) and activity in corneal epithelial cells(P<0.01). Notably, the SOD1 activity level in the H2 group was approximately 2.5-fold greater than that in the saline group.CONCLUSION: H2 treatment suppresses inflammation and neovascularization in the injured cornea and indirectly suppresses oxidative insult to the cornea by upregulating the SOD1 enzyme protein level and activity. 展开更多
关键词 HYDROGEN alkali burn Cu Zn superoxide dismutase low-vacuum scanning electron microscopy rats
A new recombinant MS-superoxide dismutase alleviates 5-fluorouracil-induced intestinal mucositis in mice 认领
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作者 Xiao-xia Yan Hai-long Li +6 位作者 Yi-ting Zhang Shou-yan Wu Heng-lei Lu Xiao-lu Yu Fan-guo Meng Jian-hua Sun Li-kun Gong 《中国药理学报:英文版》 SCIE CAS CSCD 2020年第3期348-357,共10页
Intestinal mucositis is a common side effect of anticancer regimens that exerts a negative impact on chemotherapy.Superoxide dismutase(SOD)is a potential therapy for mucositis but efficient product is not available be... Intestinal mucositis is a common side effect of anticancer regimens that exerts a negative impact on chemotherapy.Superoxide dismutase(SOD)is a potential therapy for mucositis but efficient product is not available because the enzyme is degraded following oral administration or induces an immune reaction after intravascular infusion.Multi-modified Stable Anti-Oxidant Enzymes?(MS-AOE?)is a new recombinant SOD with better resistance to pepsin and trypsin.We referred it as MS-SOD to distinguish from other SODs.In this study we investigated its potential to alleviate 5-FU-induced intestinal injury and the mechanisms.An intestinal mucositis model was established in C57/BL6 mice by 5-day administration of 5-FU(50 mg/kg every day,ip).MS-SOD(800 IU/10 g,ig)was given once daily for 9 days.5-FU caused severe mucositis with intestinal morphological damage,bodyweight loss and diarrhea;MS-SOD significantly decreased the severity.5-FU markedly increased reactive oxygen species(ROS)and inflammatory cytokines in the intestine which were ameliorated by MS-SOD.Furthermore,MS-SOD modified intestinal microbes,particularly reduced Verrucomicrobia,compared with the 5-FU group.In Caco2 cells,MS-SOD(250–1000 U/mL)dose-dependently decreased tBHP-induced ROS generation.In RAW264.7 cells,MS-SOD(500 U/mL)had no effect on LPS-induced inflammatory cytokines,but inhibited iNOS expression.These results demonstrate that MS-SOD can scavenge ROS at the initial stage of injury,thus play an indirect role in anti-inflammatory and barrier protein protection.In conclusion,MS-SOD attenuates 5-FU-induced intestinal mucositis by suppressing oxidative stress and inflammation,and influencing microbes.MS-SOD may exert beneficial effect in prevention of intestinal mucositis during chemotherapy in clinic. 展开更多
关键词 manganese superoxide DISMUTASE 5-FLUOROURACIL chemotherapy INTESTINAL MUCOSITIS DIARRHEA oxidative stress cytokines INTESTINAL microbes
Assessment of antioxidant status of women with polycystic ovarian syndrome 认领
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作者 Olufisayo Grace Oyebanji Modupe Fisayo Asaolu 《亚太生殖杂志:英文版》 2020年第1期9-15,共7页
Objective:To determine the antioxidant status of females with polycystic ovarian syndrome.Methods:Blood samples of 85 females(45 newly diagnosed polycystic ovarian syndrome patients and 40 apparently healthy subjects)... Objective:To determine the antioxidant status of females with polycystic ovarian syndrome.Methods:Blood samples of 85 females(45 newly diagnosed polycystic ovarian syndrome patients and 40 apparently healthy subjects)between 25 and 45 years of age were obtained from Ekiti State University Teaching Hospital,Ado Ekiti,Ekiti State,Nigeria.Spectrophotometry was used to evaluate the plasma concentrations of malondialdehyde,vitamin A,C and E,reduced glutathione and activities of glutathione peroxidase,catalase and superoxide dismutase.Results:The concentrations of malondialdehyde and glutathione peroxidase activity significantly increased(P<0.05)in patients with polycystic ovarian syndrome compared with the healthy controls,while concentrations of reduced glutathione,vitamins A,C,E and activities of catalase and superoxide dismutase of patients with polycystic ovarian syndrome showed significant decrease(P<0.05)compared to the healthy controls.This study showed that oxidative stress may assume a role in the pathogenesis of polycystic ovarian syndrome.There were significant negative correlations between malondialdehyde and superoxide dismutase,catalase(P<0.01)and vitamin A(P<0.05),while there was a significant positive correlation(P<0.01)between malondialdehyde and glutathione peroxidase.In addition,vitamins A(P<0.05),C(P<0.01)and E(P<0.01)showed significant positive correlations with catalase antioxidant enzyme.However,vitamins C and E showed significant positive correlation(P<0.05)among each other.Conclusions:The involvement of antioxidants in the management of polycystic ovarian syndrome may be helpful as secondary therapy to prevent oxidative damage and may be used as a potential approach to overcome metabolic as well as reproductive disorders associated with infertility in polycystic ovarian syndrome. 展开更多
关键词 MALONDIALDEHYDE Reduced GLUTATHIONE Vitamin A C and E GLUTATHIONE peroxidase CATALASE Superoxide DISMUTASE POLYCYSTIC OVARY syndrome
Quantitative analysis by reversed-phase high-performance liquid chromatography and retinal neuroprotection after topical administration of moxonidine 认领
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作者 Qian Zhang Mei-Fang Chu +5 位作者 Yan-Hong Li Chun-Hua Li Run-Jia Lei Si-Cen Wang Bao-Jun Xiao Jian-Gang Yang 《国际眼科杂志:英文版》 SCIE CAS 2020年第3期390-398,共9页
AIM:To determine moxonidine in aqueous humor and iris-ciliary body by reversed-phase high performance liquid chromatography(RP-HPLC),and to evaluate the retinal neuroprotective effect after topical administration with... AIM:To determine moxonidine in aqueous humor and iris-ciliary body by reversed-phase high performance liquid chromatography(RP-HPLC),and to evaluate the retinal neuroprotective effect after topical administration with moxonidine in a high intraocular pressure(IOP)model.METHODS:The eyes of albino rabbits were administered topically and ipsilaterally with 0.2%moxonidine.A RPHPLC method was employed for the identification and quantification of moxonidine between 2 and 480 min,which presented in the aqueous humor and iris-ciliary body.Flash electroretinography(F-ERG)amplitude and superoxide dismutase(SOD)level were measured between day 1 and day 15 after topical administration with moxonidine in a rabbit model of high IOP.Histological and ultrastructural observation underwent to analyze the changes of retinal morphology,the inner retinal layers(IRL)thickness,and retinal ganglion cell(RGC)counting.RESULTS:Moxonidine was detectable between 2 and 480 min after administration,and the peak concentration developed both in the two tissues at 30 min,0.51μg/m Lin aqueous humor and 1.03μg/g in iris-ciliary body.In comparison to control,F-ERG b-wave amplitude in moxonidine eyes were significantly differences between day 3 and day 15(P<0.01)in the high IOP model;SOD levels were significantly higher at all time-points(P<0.01)with a maximum level of 20.29 U/mgprot at day 15;and RGCs were significantly higher(P<0.05).CONCLUSION:Moxonidine is a viable neuroprotective agent with application to high IOP model.All layers of retina,including RGC layer,retinal nerve fiber layer and INL,are more preserved after moxonidine administration.SOD plays a neuroprotective role in ocular hypertension-mediated RGC death. 展开更多
关键词 REVERSED-PHASE high-performance liquid chromatography MOXONIDINE RETINAL GANGLION cell NEUROPROTECTION superoxide DISMUTASE
Possible mechanisms of lycopene amelioration of learning and memory impairment in rats with vascular dementia 认领
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作者 Ning-Wei Zhu Xiao-Lan Yin +4 位作者 Ren Lin Xiao-Lan Fan Shi-Jie Chen Yuan-Ming Zhu Xiao-Zhen Zhao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期332-341,共10页
Oxidative stress is involved in the pathogenesis of vascular dementia. Studies have shown that lycopene can significantly inhibit oxidative stress;therefore, we hypothesized that lycopene can reduce the level of oxida... Oxidative stress is involved in the pathogenesis of vascular dementia. Studies have shown that lycopene can significantly inhibit oxidative stress;therefore, we hypothesized that lycopene can reduce the level of oxidative stress in vascular dementia. A vascular dementia model was established by permanent bilateral ligation of common carotid arteries. The dosage groups were treated with lycopene(50, 100 and 200 mg/kg) every other day for 2 months. Rats without bilateral carotid artery ligation were prepared as a sham group. To test the ability of learning and memory, the Morris water maze was used to detect the average escape latency and the change of search strategy. Hematoxylin-eosin staining was used to observe changes of hippocampal neurons. The levels of oxidative stress factors, superoxide dismutase and malondialdehyde, were measured in the hippocampus by biochemical detection. The levels of reactive oxygen species in the hippocampus were observed by dihydroethidium staining. The distribution and expression of oxidative stress related protein, neuron-restrictive silencer factor, in hippocampal neurons were detected by immunofluorescence histochemistry and western blot assays. After 2 months of drug administration,(1) in the model group, the average escape latency was longer than that of the sham group, and the proportion of straight and tend tactics was lower than that of the sham group, and the hippocampal neurons were irregularly arranged and the cytoplasm was hyperchromatic.(2) The levels of reactive oxygen species and malondialdehyde in the hippocampus of the model group rats were increased, and the activity of superoxide dismutase was decreased.(3) Lycopene(50, 100 and 200 mg/kg) intervention improved the above changes, and the lycopene 100 mg/kg group showed the most significant improvement effect.(4) Neuron-restrictive silencer factor expression in the hippocampus was lower in the sham group and the lycopene 100 mg/kg group than in the model group.(5) The above data indicate that lycopene 100 mg/kg co 展开更多
关键词 bilateral carotid artery ligation LYCOPENE hippocampus learning and memory MALONDIALDEHYDE neuron neuron-restrictive SILENCER factor reactive oxygen species superoxide DISMUTASE
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Evaluation of Oxidative Stress and Antioxidant Level of Stroke Patients in Osun State, South-Western Nigeria 认领
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作者 I. Akinlua M. F. Asaolu +1 位作者 O. C. Ojo G. O. Oyebanji 《生物科学与医学(英文)》 2019年第5期189-194,共6页
Stroke is a leading cause of morbidity and mortality worldwide. Oxidative stress which is as a result of increased activity of free radicals and decreased antioxidant mechanism plays a major role in the pathogenesis o... Stroke is a leading cause of morbidity and mortality worldwide. Oxidative stress which is as a result of increased activity of free radicals and decreased antioxidant mechanism plays a major role in the pathogenesis of stroke. This study determined the plasma level of malondialdehyde (MDA), a marker of lipid peroxidation and oxidative stress and enzymatic Antioxidants namely, superoxide dismutase (SOD), Glutathione peroxidase (GPx), and Catalase (CAT) in sixty stroke patients of both sexes attending Wesley Guide Hospital, Obafemi Awolowo University Teaching Hospital, Ilesa, Osun State. An equal number of healthy subjects were used as control. The result of this study revealed a significantly (p < 0.05) high plasma level of MDA in stroke patients when compared with the control subjects. Plasma level of SOD in stroke patients was significantly higher (p < 0.05) than the control subjects while plasma GPx and CAT level were found to be significantly lower (p < 0.05) in stroke patients than the control subjects. The result of the study indicated high level of lipid peroxidation and oxidative stress in stroke patients and high degree of free radicals presence as indicated by the high level of SOD. 展开更多
关键词 Stroke MALONDIALDEHYDE CATALASE Superoxide DISMUTASE GLUTATHIONE PEROXIDASE Lipid PEROXIDATION
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Seminal Antioxidant Capacity to Oxidative Stress Induced by Electromagnetic Waves Emitting from Cellular Phones on Sperm Quality: An <i>in Vitro</i>Simulation Model 认领
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作者 Eugene Baah Christian Obirikorang +4 位作者 Richard Harry Asmah Emmanuel Acheampong Enoch Odame Anto Michael Bright Yakass Dickson Mawusi 《生殖科学(英文)》 2019年第4期94-105,共12页
With the increasing use of electronic gadgets, the effects of electromagnetic waves on sperm quality have been reported. This study evaluated the capacity of seminal antioxidants to protect spermatozoa from the effect... With the increasing use of electronic gadgets, the effects of electromagnetic waves on sperm quality have been reported. This study evaluated the capacity of seminal antioxidants to protect spermatozoa from the effects of exposure to non-ionizing cellular phone radiation on markers of semen quality and DNA integrity in an in-vitro model. A total of seventy-three (73) patients attending a fertility centre in Sakumono, Tema metropolis were recruited for this study. About 2 - 3 mls of freshly ejaculated semen was neatly collected by masturbation with consent and analysed according to the 2010 WHO standard semen analysis. A portion of Semen was exposed to non-ionizing cellular phone radiation for three (3) hours. Superoxide dismutase (SOD) activity levels and DNA fragmentation index (DFI) were determined before and after exposure. Data was entered and analysed using SPSS version 20.00 and Graph pad prism. DFI was positively associated with immobility (r = 0.233;p = 0.05) and negatively associated with percentage progressive motility (r = -0.258;p = 0.028), percentage total motility (r = -0.233;p = 0.05) and SOD levels (r = -0.270;p = 0.028). There was a significant positive linear relationship between SOD and progressive motility (r = 0.451;p < 0.0001), vitality (r = 0.725;p < 0.0001) and total motility (r = 0.705, p = 0.05). Higher seminal SOD level is protective against the damaging effects of electromagnetic waves on sperm quality. 展开更多
关键词 DNA FRAGMENTATION Index Superoxide DISMUTASE SEMEN Non-Ionizing RADIATIONS Cellular Phones
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Reactive oxygen species mediates a metabolic memory of high glucose stress signaling in bovine retinal pericytes 认领
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作者 Li-Wei Zhang Han Zhao Bai-Hua Chen 《国际眼科杂志:英文版》 SCIE CAS 2019年第7期1067-1074,共8页
AIM: To investigate the role of reactive oxygen species (ROS) and antioxidant mechanism underlying the metabolic memory of bovine retinal pericytes (BRPs) induced by high glucose.METHODS: Effects of high glucose level... AIM: To investigate the role of reactive oxygen species (ROS) and antioxidant mechanism underlying the metabolic memory of bovine retinal pericytes (BRPs) induced by high glucose.METHODS: Effects of high glucose levels and culture time on BRPs viability were evaluated by CCK-8. BRPs were grown in high-glucose media (30 mmol/L) for 4d followed by culture in normal glucose condition (5.6 mmol/L) for 4d in an experimental group. In contrast, in negative and positive control groups, BRPs were grown in either normal-glucose media or high-glucose media for 8d, respectively. The ROS levels, apoptosis, the expression and activity of manganese superoxide dismutase (MnSOD) in BRPs, as well as the protective effect of adeno-associated viral (AAV)-mediated over expression of MnSOD were determined separately by DCHFA, ELISA and Western blot.RESULTS: Comparing the result of cells apoptosis, activity and protein expression of MnSOD and caspase-3, the cell culture system that exposed in sequence in 30 mmol/L and normal glucose for 4d was demonstrated as a suitable model of metabolic memory. Furthermore, delivery of antioxidant gene MnSOD can decrease BRPs apoptosis, reduce activated caspase-3, and reverse hyperglycemic memory by reducing the ROS of mitochondria.CONCLUSION: Increased ROS levels and decreased MnSOD levels may play important roles in pericyte loss of diabetic retinopathy. BRPs cultured in high glucose for 4d followed by normal glucose for 4d could be an appropriate model of metabolic memory. rAAV-MnSOD gene therapy provides a promising strategy to inhibit this blinding disease. 展开更多
关键词 diabetic RETINOPATHY metabolic memory manganese superoxide DISMUTASE molecular THERAPEUTICS reactive oxygen species
Visual dual chemodynamic/photothermal therapeutic nanoplatform based on superoxide dismutase plus Prussian blue 认领
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作者 Shan Lei Jinxing Chen +2 位作者 Kun Zeng Mozhen Wang Xuewu Ge 《纳米研究:英文版》 SCIE EI CAS CSCD 2019年第5期1071-1082,共12页
Enzyme-based anticancer therapy is more attractive for the less side effect than conventional chemotherapy.However,the poor stability and low membrane permeability of enzymes during the intracellular delivery are cons... Enzyme-based anticancer therapy is more attractive for the less side effect than conventional chemotherapy.However,the poor stability and low membrane permeability of enzymes during the intracellular delivery are constraints for its practical applications.In this work,we synthesized novel near-infrared (NIR)-responsive core-shell-structured Prussian blue@fibrous SiO2 (PBFS) nanoparticles as the carrier of superoxide dismutase (SOD) and a glutathione (GSH)-activated Fenton reagent (DiFe).The PBFS nanoparticles are further modified with aGSH-responsive cationic polymer (poly(2-(acryloyloxy)-N,N-dimethyl-N-(4-(((2-((2-(((4-methyl-2-oxo-2H-chromen-7-yl)carbamoyl)oxy)ethyl)disulfaneyl)ethoxy)carbonyl)amino)benzyl)ethan-1-aminium,PSS) containing disulfide bonds and fluorophores.After SOD and DiFe are loaded on the PBFS-PSS nanoparticles,dual chemodynamic/photothermal therapeutic nanoparticulate systems (PBFS-PSS/DiFe/SOD) are obtained.In vitro experiments show that PBFS-PSS/DiFe/SOD nanoparticles have good biocompatibility and can be tracked under fluorescence microscope during the intracellular delivery process in MCF-7 tumor cells due to the GSH-activated release of fluorophores.They also exhibit high efficiency in NIR photothermal conversion and GSH-activated Fenton reaction in tumor cells,thus achieving high-efficient killing effect of tumor cells based on the combination of photothermal and chemodynamic therapeutic performance (PTT and CDT).This work offers a novel pathway to construct a visual multifunctional nanomedicine platform for future cancer therapy. 展开更多
关键词 copper-zinc superoxide DISMUTASE Fenton REAGENT Prussian blue NEAR-INFRARED (NIR) PHOTOTHERMAL therapy fluorescence
芡实超微粉的体内降血脂功效 认领
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作者 王岁岁 张汆 +2 位作者 戚良号 杨杰 袁怀波 《食品研究与开发》 CAS 北大核心 2019年第10期65-69,共5页
采用不同剂量的芡实超微粉对高脂模型小鼠进行灌胃,研究芡实超微粉对高脂模型小鼠血脂四项指标及血清和肝组织中相关酶类的影响。结果发现,与高脂模型组相比,灌胃芡实超微粉组小鼠体重明显降低,肝脏指数和动脉粥样硬化指数也明显降低;... 采用不同剂量的芡实超微粉对高脂模型小鼠进行灌胃,研究芡实超微粉对高脂模型小鼠血脂四项指标及血清和肝组织中相关酶类的影响。结果发现,与高脂模型组相比,灌胃芡实超微粉组小鼠体重明显降低,肝脏指数和动脉粥样硬化指数也明显降低;小鼠血清总胆固醇(total cholesterol,TC)、甘油三脂(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)水平均明显降低,高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)水平则显著升高;灌胃芡实超微粉低、中、高剂量组小鼠的总超氧化物歧化酶(total superoxide dismutase,T-SOD)和肝酯酶(hepatic lipase,HL)活力均高于高脂模型组,而丙二醛(malondialdehyde,MDA)含量要低于高脂模型组。由此可知,芡实超微粉可降低高血脂症小鼠血脂水平,改善其因摄入过多脂质而导致的肝脏脂肪堆积和动脉粥样硬化,并对高血脂症引起的脂质过氧化损伤具有防护作用。 展开更多
关键词 芡实超微粉 高血脂症 歧化酶 肝脂酶 丙二醛 防护作用
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Tinospora cordifolia attenuates antipsychotic drug induced hyperprolactinemia in Wistar rats 认领
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作者 Prashant Tiwari Sunil Kumar Dubey Pratap Kumar Sahu 《亚太生殖杂志(英文版)》 2019年第3期132-140,共9页
Objective: To evaluate the anti-hyperprolactinemic effect of methanolic extract of Tinospora cordifolia against antipsychotic/neuroleptic drug induced hyperprolactinemia. Methods: A total of 48 Wistar albino rats were... Objective: To evaluate the anti-hyperprolactinemic effect of methanolic extract of Tinospora cordifolia against antipsychotic/neuroleptic drug induced hyperprolactinemia. Methods: A total of 48 Wistar albino rats were chosen in the study. To induce hyperprolactinemia, haloperidol at 5 mg/kg/day was intraperitoneally administered for 16 continuous days and sulpiride at 20 mg/kg/day was administered intraperitoneally for 28 continuous days. Methanolic extract of Tinospora cordifolia at 200 mg/kg/day and 400 mg/kg/day were administered orally 30 min before administration of haloperidol and sulpiride for 16 and 28 days, respectively. Then, we had evaluated prolactin, dopamine and antioxidant status in the treatment group as compared to haloperidol and sulpiride. Results: There was a significant (P<0.05) increase in serum prolactin level and decrease in dopamine level in the haloperidol and sulpiride treated animals. However, methanolic extract of Tinospora cordifolia significantly (P<0.05) decreased serum prolactin level and increased brain dopamine level. Further, superoxide dismutase and catalase level were also decreased significantly in the haloperidol and sulpiride treated groups as compared to those of the control group and the antioxidant status was restored significantly on treatment with methanolic extract of Tinospora cordifolia. Furthermore, methanolic extract of Tinospora cordifolia also reduced total leukocyte count, and increased red blood cell count and hemoglobin concentration. In addition, the spleen did not show signs of infection or inflammation in the experiments. Conclusions: Methanolic extract of Tinospora cordifolia has a significant anti-hyperprolactinemic effect which may be attributed to neuroprotective and antioxidant effects of its signature constituents like stepharanine. 展开更多
关键词 ANTIPSYCHOTIC NEUROLEPTIC Dopamine PROLACTIN Superoxide DISMUTASE CATALASE
Binding studies of trans-resveratrol with superoxide dismutase (SOD1): Docking assessment and Thermoanalysis 认领
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作者 Janhvi Dureja Renu Chadha +2 位作者 Maninder Karan Akshita Jindal Kunal Chadha 《制药与生物制药研究杂志(英文)》 2019年第1期21-27,共7页
The binding pursuits of trans-resveratrol(t-RSV),an amazing health supplement are investigated with an antioxidant enzyme,superoxide dismutase(SOD1).The aim of the study is to dock t-RSV on the adrenaline binding site... The binding pursuits of trans-resveratrol(t-RSV),an amazing health supplement are investigated with an antioxidant enzyme,superoxide dismutase(SOD1).The aim of the study is to dock t-RSV on the adrenaline binding site on SOD1 in order to explore its potential to act as a safety net against amyotrophic lateral sclerosis(ALS),a fatal neurodegenerative disorder that affects motor neurons.In silico GLIDE docking methodology and in vitro microcalorimetry technique is utilized for the investigation of binding parameters of t-RSV with SOD1.The study provides useful and distinct information about the amino acids involved in the interactions at molecular level along with the nature of forces involved in binding of t-RSV with SOD1.The docking analysis using the scoring functions of Schrodinger’s Glide package depicts that GLU100,PRO28,LYS23,TRP32 residues of the peptide backbone on SOD1 interact with phenolic groups of t-RSV.The information on thermodynamic parameters,i.e.binding constant(Kb),free energy(△G)and enthalpy(△H)generated through calorimetric titrations suggests that the reaction between t-RSV and SOD1 is spontaneous and exothermic.Both the studies are found to be in close agreement with each other based as far as the magnitude of binding constant(Kb=9.9×10^4)is concerned. 展开更多
关键词 TRANS-RESVERATROL superoxide DISMUTASE DOCKING MICROCALORIMETRY binding constant free energy
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The Role of Serum Ceruloplasmin and Oxidative Stress Markers in Primary Open Angle Glaucoma 认领
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作者 Abdul Monem Al-Sayed Abou Sharkh Waled Mahdy Nada +1 位作者 Doaa Attia Abdel Moety Hany Ahmad Elkattawy 《眼科学期刊(英文)》 2018年第1期18-25,共8页
Purpose: The study aimed at evaluation of the role of ceruloplasmin (A protein involved in iron homeostasis and can inactivate free radicals) and other oxidative stress markers as superoxide dismutase (SOD), malondial... Purpose: The study aimed at evaluation of the role of ceruloplasmin (A protein involved in iron homeostasis and can inactivate free radicals) and other oxidative stress markers as superoxide dismutase (SOD), malondialdehyde (MDA) and catalase activity (CAT) in primary open angle glaucoma (POAG). Methods: This observational case control study included 90 persons divided into 3 equal groups: group A of 30 normal persons as a control group, group B of 30 patients of untreated (POAG) (firstly diagnosed) by the clinical characters including measuring intraocular pressure (IOP), optic disc cupping and visual field changes and group C of 30 patients of POAG under medical treatment by topical anti-glaucomatous drugs. Serum ceruloplasmin, superoxide dismutase, malondialdehyde and catalase activity were measured in all groups, statistical analysis of the data was performed. Results: In a comparison to group A of control, serum ceruloplasmin decreased significantly in group B of untreated POAG (20.95 ± 6.01) mg-dl and in group C of POAG under treatment (22.15 ± 6.14) mg-dl (P 0.05). Also, serum superoxide dismutase increased significantly in group B (2.23 ± 0.4) and in group C (2.19 ± 0.38) U-ml (P 0.05). Serum malondialdehyde increased significantly in group B (3.82 ± 0.74) nmol-ml and in group C (3.55 ± 0.73) nmol-ml (P 0.05). Serum catalase decreased significantly in group B (17.97 ± 2.75) U-ml and in group C (18.75 ± 2.33) U-ml in a comparison to the control group A (22.67 ± 3.05) U-ml (P 0.05). Conclusions: Serum ceruloplasmin level and the antioxidant (CAT) activity significantly decreased, while serum levels of SOD, MDA significantly increased in cases of POAG. This may indicate the need for addition of anti-oxidative stress therapy in combination with the anti-glaucomatous drugs. Monitoring these markers can be considered good indicators for determination of the oxidative stress condition in such cases. 展开更多
关键词 CERULOPLASMIN Primary Open Angle GLAUCOMA Oxidative Stress MARKERS Superoxide DISMUTASE (SOD) MALONDIALDEHYDE (MDA) and CATALASE Activity (CAT)
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Lipid Peroxidation and Some Antioxidant Enzymes Evaluation in Apple Cider Vinegar (ACV) Treated Male and Female Wistar Rats Exposed to Chronic Restraint Stress 认领
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作者 R. A. Abdulrauf F. A. Dawud +6 位作者 N. S. Emmanuel H. D. Muhammad A. S. Dange B. A. David A. E. Ogweje A. U. Alexander M. Yahuza 《酶研究进展(英文)》 2018年第3期21-28,共8页
This study was designed to assess the effect to apple cider vinegar (ACV) on oxidative stress biomarkers in male and female Wistar rats exposed to chronic restraint stress. Severe and persistent stress elevates reacti... This study was designed to assess the effect to apple cider vinegar (ACV) on oxidative stress biomarkers in male and female Wistar rats exposed to chronic restraint stress. Severe and persistent stress elevates reactive oxygen species (ROS) production by metabolic and physiological processes;causing cellular damage. Thirty (30) Adult Wistar rats of both sexes weighing about 150 - 200 g were divided into 3 groups each consisting of a male and female subgroup and given the following treatments once a day for 21 days: Normal control group received 0.5 ml distilled water orally, the restraint stress (RS) group was exposed to chronic restraint stress 6 hours daily while the Apple cider vinegar (ACV)-treated group received 4 ml/kg of apple cider vinegar orally in addition to chronic restraint stress 6 hours daily. The rats were sacrificed after the experimental period and blood was collected via cardiac puncture for assessing oxidative stress biomarkers. ACV (4 ml/kg) treatment decreased lipid peroxidation (MDA) and serum catalase (CAT) activity while upregulating endogenous superoxide dismutase (SOD) activity. The findings of this study show that the female Wistar rats are more predisposed to the antioxidant effect of ACV than the males. 展开更多
关键词 Apple CIDER VINEGAR Superoxide DISMUTASE MALONDIALDEHYDE CATALASE
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苏木对糖尿病大血管病变中SOD、TNF-α、MCP-1的影响 认领 被引量:1
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作者 刘影哲 温宇婷 +3 位作者 闫博馨 丁家喻 王雪 潘祥宾 《现代生物医学进展》 CAS 2018年第14期2637-2640,共4页
目的:探讨苏木对糖尿病大血管病变大鼠血清超氧化物歧化酶(SOD)、肿瘤坏死因子α(TNF-α)、单核细胞趋化因子1(MCP-1)水平的影响。方法:将24只雄性SD大鼠随机分为4组,每组6只,分别为对照组、模型组、α-硫辛酸组和苏木组。除去... 目的:探讨苏木对糖尿病大血管病变大鼠血清超氧化物歧化酶(SOD)、肿瘤坏死因子α(TNF-α)、单核细胞趋化因子1(MCP-1)水平的影响。方法:将24只雄性SD大鼠随机分为4组,每组6只,分别为对照组、模型组、α-硫辛酸组和苏木组。除去对照组,余下的18只大鼠单次腹腔注射STZ50 mg/kg制备糖尿病大鼠模型,对照组予以注射等体积的柠檬酸-柠檬酸钠缓冲液。成功造模后,苏木组给予0.15 g/kg/d的苏木,α-硫辛酸组给予20 mg/kg/d的α-硫辛酸,每日1次给药,连续给药8周。8周末,检测和比较两组大鼠血清中SOD、TNF-α、MCP-1水平的变化。结果:治疗后,苏木组及α-硫辛酸组血清SOD含量均高于模型组(P〈0.05),但苏木组和α-硫辛酸组组间比较无统计学差异(P〉0.05);苏木组及α-硫辛酸组血清TNF-α、MCP-1含量均显著低于模型组(P〈0.05),与对照组没有明显差别(P〉0.05)。结论:苏木可提高糖尿病大血管病大鼠血清SOD含量,降低血清TNF-α、MCP-1含量。 展开更多
关键词 苏木 糖尿病大血管病变 Superoxide DISMUTASE Tumor NECROSIS factor-α MONOCYTE CHEMOATTRACTANT protein-1
The effect of curcumin as an antioxidant on cochlea fibroblasts in ototoxic rat models 认领
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作者 Tengku Siti Hajar Haryuna Agustinus Hamonangan Winston Purba +1 位作者 Farhat Farhat Soehartono Taat Putra 《中国药学:英文版》 CAS CSCD 2018年第12期847-854,共8页
Aminoglycosides (e.g. Gentamicin) are ototoxic drugs and widely prescribed due to their effective antimicrobial actions and affordable prices. This study focused on determining protective effect of curcumin against th... Aminoglycosides (e.g. Gentamicin) are ototoxic drugs and widely prescribed due to their effective antimicrobial actions and affordable prices. This study focused on determining protective effect of curcumin against the damage caused by aminoglycosides. We aimed to demonstrate the potential of curcumin as an antioxidant to increase the expressions of superoxide dismutase (SOD) in fibroblasts of cochlea lateral wall in ototoxic rat models. The experiment was conducted with randomized post test-only control group design by using 32 male Rattusnorvegicus adults which received a combination of gentamicin and curcumin with different durations and doses. Then, the rats underwent terminations and immunohistochemical assay to determine the expression of SOD. The rats receiving gentamicin injection showed significantly decreased expression of SOD (P<0.05), and the administration of curcumin before and after the gentamicin injection showed significantly increased expression of SOD (P<0.05). Collectively, we showed that curcumin was an antioxidant against oxidative stress due to ototoxicity evidenced by the expression of SOD. 展开更多
关键词 CURCUMIN ANTIOXIDANT GENTAMICIN Superoxide DISMUTASE Preventive Rat Experimental
Superoxide dismutase mimetic ability of Mn-doped ZnS QDs 认领
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作者 Xiangling Ren Mingqian Wang +6 位作者 Xing He Zheng Li Jing Zhang Wei Zhang Xudong Chen Hong Ren Xianwei Meng 《中国化学快报:英文版》 SCIE CAS CSCD 2018年第12期1865-1868,共4页
Superoxide dismutase(SOD) is an important antioxidant enzyme in the body. SOD has special physiological activity and is the primary substance for scavenging free radicals in living organisms.However, the expensive and... Superoxide dismutase(SOD) is an important antioxidant enzyme in the body. SOD has special physiological activity and is the primary substance for scavenging free radicals in living organisms.However, the expensive and complex extraction processes, low SOD yield, as well as difficult to store at room temperature have seriously hindered its application pace. Herein, the enzyme mimetic function of Mn-doped ZnS quantum dots(QDs) was discovered. The improved Marklund and McCord method both showed that Mn-doped ZnS QDs possess intrinsic SOD-like activity. The effects of temperature and pH on the mimetic enzyme activity of Mn-doped ZnS QDs have been investigated compared with SOD enzymes.The low cost and easy to synthesize white Mn-doped ZnS QDs with good biocompatibility are expected to be used as a new type of SOD nanozymes in the biology-relevant fields. 展开更多
关键词 MN-DOPED ZNS QDS Superoxide DISMUTASE Nanozymes Marklund method PYROGALLOL
Significance of Cu/Zn-Superoxide Dismutase Levels in Hemodialysis Patients: A Mini Review 认领
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作者 Yuya Nakamura Masahiro Inagaki +8 位作者 Sachiyo Kenmotsu Shiho Yamadera Isao Ohsawa Hiromichi Gotoh Yoshikazu Goto Naoki Sato Tatsunori Oguchi Mayumi Tsuji Yuji Kiuchi 《炎症(英文)》 2017年第2期9-13,共5页
Cu/Zn-superoxide dismutase (Cu/Zn-SOD) is an enzyme that is ubiquitously present in the cytoplasm and causes dismutation of superoxide radicals, therefore Cu/Zn-SOD is primarily used as an antioxidant marker. Levels o... Cu/Zn-superoxide dismutase (Cu/Zn-SOD) is an enzyme that is ubiquitously present in the cytoplasm and causes dismutation of superoxide radicals, therefore Cu/Zn-SOD is primarily used as an antioxidant marker. Levels of Cu/Zn-SOD are higher in the serum of hemodialysis patients than in serum of healthy volunteers. The increase of serum Cu/Zn-SOD levels is related to the decrease of kidney function with aging and arteriosclerosis in hemodialysis patients. Moreover, infection, vascular puncture, and hemostasis may be related to the increase in serum Cu/Zn-SOD levels. As it is associated with numerous factors in hemodialysis patients, Cu/Zn-SOD may serve as a complex marker for arteriosclerosis, vascular, and inflammatory conditions. It is important to investigate various agents that decrease serum Cu/Zn-SOD levels to improve the life-span of hemodialysis patients. 展开更多
关键词 Cu/Zn-Superoxide DISMUTASE HEMODIALYSIS Complex MARKER
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