期刊文献+
共找到215,268篇文章
< 1 2 250 >
每页显示 20 50 100
Mechanical force drives the polarization and orientation of cells 预览
1
作者 Shijie He Xiaomeng Li Baohua Ji 《力学学报:英文版》 SCIE EI CAS CSCD 2019年第2期275-288,共14页
Collective cells are organized to form specific patterns which play important roles in various physiological and pathological processes, such as tissue morphogenesis, wound healing, and cancer invasion. Compared to si... Collective cells are organized to form specific patterns which play important roles in various physiological and pathological processes, such as tissue morphogenesis, wound healing, and cancer invasion. Compared to single cell behaviors, which has been intensively studied from many aspects (cell migration, adhesion, polarization, proliferation, etc.) and at various scales (molecular, subcellular, and cellular), the multiple cell behaviors are relatively less understood, particularly in a quantitative manner. In this paper, we will present our recent studies of collective polarization and orientation of multiple cells through both experimental measurement and theoretical modeling, including those cell behaviors on/in 2D and 3D substrate/tissue. We find that the collective cell behaviors, including polarization, alignment and migration are closely related to local stress states in cell layer or tissue, which demonstrate the crucial roles of mechanical forces in the living organisms. Specifically, the cells prefer to polarize and align along the maximum principal stress in the cell layer, and the aspect ratio of cell increases with the in-plane maximum shear stress, suggesting that the maximum shear stress is the underlying driving force of cell polarization and orientation. This theory of stress-driven cell behaviors of polarization and orientation provides a new perspective for understanding cell behaviors in living organisms and the guideline for tissue engineering in biomedical applications. 展开更多
关键词 COLLECTIVE CELL behaviors CELL POLARIZATION CELL ALIGNMENT Quantification CELL MECHANOSENSING
在线阅读 下载PDF
Effects of neural stem cell transplantation on the motor function of rats with contusion spinal cord injuries:a meta-analysis 预览
2
作者 Kai Qian Tuo-Ye Xu +7 位作者 Xi Wang Tao Ma Kai-Xin Zhang Kun Yang Teng-Da Qian Jing Shi Li-Xin Li Zheng Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期748-758,共11页
Objective:To judge the efficacies of neural stem cell(NSC)transplantation on functional recovery following contusion spinal cord injuries(SCIs).Data sources:Studies in which NSCs were transplanted into a clinically re... Objective:To judge the efficacies of neural stem cell(NSC)transplantation on functional recovery following contusion spinal cord injuries(SCIs).Data sources:Studies in which NSCs were transplanted into a clinically relevant,standardized rat model of contusion SCI were identified by searching the PubMed,Embase and Cochrane databases,and the extracted data were analyzed by Stata 14.0.Data selection:Inclusion criteria were that NSCs were used in in vivo animal studies to treat contusion SCIs and that behavioral assessment of locomotor functional recovery was performed using the Basso,Beattie,and Bresnahan lo-comotor rating scale.Exclusion criteria included a follow-up of less than 4 weeks and the lack of control groups.Outcome measures:The restoration of motor function was assessed by the Basso,Beattie,and Bresnahan locomotor rating scale.Results:We identified 1756 non-duplicated papers by searching the aforementioned electronic databases,and 30 full-text articles met the inclusion criteria.A total of 37 studies reported in the 30 articles were included in the meta-analysis.The meta-analysis results showed that transplanted NSCs could improve the motor function recovery of rats following contusion SCIs,to a moderate extent(pooled standardized mean difference(SMD)=0.73;95%confidence interval(CI):0.47–1.00;P<0.001).NSCs obtained from different donor species(rat:SMD=0.74;95%CI:0.36–1.13;human:SMD=0.78;95%CI:0.31–1.25),at different donor ages(fetal:SMD=0.67;95%CI:0.43–0.92;adult:SMD=0.86;95%CI:0.50–1.22)and from different origins(brain-derived:SMD=0.59;95%CI:0.27–0.91;spinal cord-derived:SMD=0.51;95%CI:0.22–0.79)had similar efficacies on improved functional recovery;however,adult induced pluripotent stem cell-derived NSCs showed no significant efficacies.Furthermore,the use of higher doses of transplanted NSCs or the administration of immunosuppressive agents did not promote better locomotor function recovery(SMD=0.45;95%CI:0.21–0.70).However,shorter periods between the contusion induction and the NSC tr 展开更多
关键词 Basso Beattie and Bresnahan locomotor rating scale cell transplantation META-ANALYSIS motor functional recovery neural regeneration neural stem cell neural stem cell transplantation rat model spinal contusion spinal cord injury
在线阅读 下载PDF
THZ1 suppresses human non-small-cell lung cancer cells in vitro through interference with cancer metabolism
3
作者 Zhu-Jun Cheng Du-Ling Miao +5 位作者 Qiu-Yun Su Xiao-Li Tang Xiao-Lei Wang Li Bin Deng Hui Donq Shi Hong-Bo Xin 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第6期814-822,共9页
Cancer cells always require more nutrients, energy, and biosynthetic activity to sustain their rapid proliferation than normal cells. Previous studies have shown the impact of THZ1, a covalerrt inhibitor of ryHirwjepe... Cancer cells always require more nutrients, energy, and biosynthetic activity to sustain their rapid proliferation than normal cells. Previous studies have shown the impact of THZ1, a covalerrt inhibitor of ryHirwjependent kinase 7 (CDK7), on transcription regulation and cell-cycle arrest in numerous (nncers, but its effects on cellular metabolism in cancer colls remain unknown. In this btudy we elucidated the anticancer mechanism of THZ1 in human non-small-cell lung cancer (NSCLC) cells. We showed that treatment with THZ1 (10 1000 nM) dose-dependently suppressed the proliferation of human NSCLC cell lines H1299, A549, H292, and H23, and markedly inhibited the migration of these NSCLC cells. Furthermore treatment with THZ1 (50 nM) arrested cell cycle at G2/M phase and induced apoptosis in these NSCLC cell lines. More importantly, we revealed that treatment with THZ1 (50 nM) blocked the glycolysis pathway but had no effect on glutamine metabolism. Wc further demonstrated that THZ1 treatment altered thp expression pattern of gluwminasc 1 (GLS1) Isoforms through promoting the ubiquitination and degradation of NUDT21. Combined treatment of THZ1 with a glutaminase inhibitor CB-839 (500 nM) exerted a more potent anti-proliferative effect in these NSCLC cell lines than treatment with TH21 or CB-83Q alone. Our results demonstrate that the inhibitory effect of THZ1 on the growth of humnn NSCLC cells is partially attributed to interfering with cancer metabolism. Thus, we provide a new potential therapeutic strategy for NSCLC treatment by combining THZ1 with the inhibitors of glutamine metabolism. 展开更多
关键词 THZ1 CDK7 inhibitor non small CELL lung cancer anti PROLIFERATIVE effect CELL-CYCLE ARREST CELL apoptosis GLYCOLYSIS pathway qlutamine metabolism CB-839
Tonsil-derived stem cells as a new source of adult stem cells 预览
4
作者 Kyung-Ah Cho Hyun Jung Lee +8 位作者 Hansaem Jeong Miri Kim Soo Yeon Jung Hae Sang Park Kyung-Ha Ryu Seung Jin Lee Byeongmoon Jeong Hyukjin Lee Han Su Kim 《世界干细胞杂志:英文版(电子版)》 2019年第8期506-518,共13页
Located near the oropharynx, the tonsils are the primary mucosal immune organ. Tonsil tissue is a promising alternative source for the high-yield isolation of adult stem cells, and recent studies have reported the ide... Located near the oropharynx, the tonsils are the primary mucosal immune organ. Tonsil tissue is a promising alternative source for the high-yield isolation of adult stem cells, and recent studies have reported the identification and isolation of tonsil-derived stem cells (T-SCs) from waste surgical tissue following tonsillectomies in relatively young donors (i.e., under 10 years old). As such, TSCs offer several advantages, including superior proliferation and a shorter doubling time compared to bone marrow-derived mesenchymal stem cells (MSCs). T-SCs also exhibit multi-lineage differentiation, including mesodermal, endodermal (e.g., hepatocytes and parathyroid-like cells), and even ectodermal cells (e.g., Schwann cells). To this end, numbers of researchers have evaluated the practical use of T-SCs as an alternative source of autologous or allogenic MSCs. In this review, we summarize the details of T-SC isolation and identification and provide an overview of their application in cell therapy and regenerative medicine. 展开更多
关键词 STEM CELL Tonsil-derived STEM CELL Differentiation ENDODERM Mesoderm ECTODERM CELL therapy
在线阅读 免费下载
Truth lies below: A case report and literature review of typical appearing polyps yet with an atypical diagnosis 预览
5
作者 Aaron Fisher Edward Yousif Marc Piper 《世界胃肠内镜杂志:英文版(电子版)》 2019年第1期54-59,共6页
BACKGROUND Enteropathy associated T-cell lymphoma (EATL) is a rare form of peripheral Tcell lymphoma and makes up less than 5% of gastrointestinal lymphomas. EATL can be divided into type 1 which is associated with ce... BACKGROUND Enteropathy associated T-cell lymphoma (EATL) is a rare form of peripheral Tcell lymphoma and makes up less than 5% of gastrointestinal lymphomas. EATL can be divided into type 1 which is associated with celiac disease, and monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL), formally type 2, which is not associated with celiac disease. CASE SUMMARY We present a 60-year-old African American female, without celiac disease, who presented with abdominal pain, diarrhea, and 30 lb. weight loss over a 3 month period. She was subsequently diagnosed with EATL throughout her entire gastrointestinal tract. She is currently undergoing chemotherapy with EOCH (Etoposide, Oncovin, Cyclophosphamide, and Hydroxydaunorubicin). EATL is most common in the Asian and Hispanic population yet the incidence in African Americans is uncertain and emphasizes the rarity of this case. A literature review was included to further emphasize similarities and differences between our case and previously reported cases of MEITL. CONCLUSION The patient was diagnosed with EATL, immunochemical testing was not conclusive for MEITL however was suggestive of the disease. 展开更多
关键词 ENTEROPATHY associated T-CELL LYMPHOMA Monomorphic epitheliotropic intestinal T-CELL LYMPHOMA Peripheral T-CELL LYMPHOMA Gastrointestinal LYMPHOMA Endoscopy Case report LITERATURE review
在线阅读 免费下载
Donor-Derived CD19-Targeted T Cell Infusion Eliminates B Cell Acute Lymphoblastic Leukemia Minimal Residual Disease with No Response to Donor Lymphocytes after Allogeneic Hematopoietic Stem Cell Transplantation 预览
6
作者 Yifei Cheng Yuhong Chen +11 位作者 Chenhua Yan Yu Wang Xiangyu Zhao Yao Chen Wei Han Lanping Xu Xiaohui Zhang Kaiyan Liu Shasha Wang Lungji Chang Lei Xiao Xiaojun Huang 《工程(英文)》 2019年第1期150-155,共6页
Leukemia relapse is still the leading cause of treatment failure after allogeneic hematopoietic stem cell transplantation (allo-HSCT) for B cell acute lymphoblastic leukemia (B-ALL). Relapsed patients with BALL after ... Leukemia relapse is still the leading cause of treatment failure after allogeneic hematopoietic stem cell transplantation (allo-HSCT) for B cell acute lymphoblastic leukemia (B-ALL). Relapsed patients with BALL after allo-HSCT have a very short median survival. Minimal residual disease (MRD) is predictive of forthcoming hematological relapse after hematopoietic stem cell transplantation (HSCT);furthermore, eliminating MRD effectively prevents relapse. Donor lymphoblastic infusion (DLI) is the main established approach to treat B-ALL with MRD after allo-HSCT. However, about one-third of patients with MRD are non-responsive to DLI and their prognosis worsens. Although donor-derived cluster of differentiation (CD)19-directed chimeric antigen receptor-modified (CAR) T cells (CART19s) can potentially cure leukemia, the efficiency and safety of infusions with these cells have not yet been investigated in patients with MRD after HSCT. Between September 2014 and February 2018, six patients each received one or more infusions of CART19s from HSCT donors. Five (83.33%) achieved MRD-negative remission, and one case was not responsive to the administration of CAR T cells. Three of the six patients are currently alive without leukemia. No patient developed acute graft-versus-host disease (aGVHD), and no patient died of cytokine release syndrome. Donor-derived CAR T cell infusions seem to be an effective and safe intervention for patients with MRD in B-ALL after allo-HSCT and for those who were not responsive to DLI. 展开更多
关键词 Donor-derived CD19-targeted T CELL INFUSION Hematopoietic stem CELL transplantation B CELL acute lymphoblastic leukemia Minimal residual disease
在线阅读 免费下载
Applications of carbon nanotubes and graphene for third-generation solar cells and fuel cells 预览
7
作者 Jianyong Ouyang 《纳米材料科学(英文版)》 CAS 2019年第2期77-90,共14页
Carbon nanotubes (CNTs) and graphene have attracted great attention since decades ago because of their interesting structure and properties and important application in many areas. They can have high conductivity, hig... Carbon nanotubes (CNTs) and graphene have attracted great attention since decades ago because of their interesting structure and properties and important application in many areas. They can have high conductivity, high specific surface area, high transparency in the visible range and high mechanical flexibility. They have important application in energy conversion systems including solar cells and fuel cells. They have been extensively studied as the transparent electrode and interfacial materials of organic solar cells (OSCs) and perovskite solar cells (PSCs). They are also used as the catalytic counter electrode of dye-sensitized solar cells (DSSCs). In addition, graphene oxide (GO) is exploited as an auxiliary binder of TiO2 paste for the mesoporous TiO2 layer of DSSCs, and GO and functionalized CNTs are adopted as gelators of gel electrolyte for quasi-solid state DSSCs. CNTs and graphene also have important application in fuel cells. They can be used as catalyst support for the oxidation of fuels or oxygen reduction reaction (ORR). CNTs and graphene, particularly when doped with nitrogen, can be directly used metal-free catalysts. This article provides a brief review on the application of CNTs and graphene in OSCs, PSCs, DSSCs and fuel cells. 展开更多
关键词 Carbon NANOTUBE GRAPHENE organic SOLAR CELL perovskite SOLAR CELL DYE-SENSITIZED SOLAR CELL ELECTROCATALYSIS
在线阅读 免费下载
Repurposing vitamin D for treatment of human malignancies via targeting tumor microenvironment
8
作者 Xu Wu Wei Hu +11 位作者 Lan Lu Yueshui Zhao Yejiang Zhou Zhangang Xiao Lin Zhang Hanyu Zhang Xiaobing Li Wanping Li Shengpeng Wang Chi Hin Cho Jing Shen Mingxing Li 《药学学报:英文版》 CSCD 2019年第2期203-219,共17页
Tumor cells along with a small proportion of cancer stem cells exist in a stromal microenvironment consisting of vasculature, cancer-associated fibroblasts, immune cells and extracellular components.Recent epidemiolog... Tumor cells along with a small proportion of cancer stem cells exist in a stromal microenvironment consisting of vasculature, cancer-associated fibroblasts, immune cells and extracellular components.Recent epidemiological and clinical studies strongly support that vitamin D supplementation is associated with reduced cancer risk and favorable prognosis. Experimental results suggest that vitamin D not only suppresses cancer cells, but also regulates tumor microenvironment to facilitate tumor repression. In this review, we have outlined the current knowledge on epidemiological studies and clinical trials of vitamin D. Notably, wesummarized and discussed the anticancer action of vitamin D in cancer cells, cancer stem cells and stroma cells in tumor microenvironment, providing a better understanding of the role of vitamin D in cancer. We presently re-propose vitamin D to be a novel and economical anticancer agent. 展开更多
关键词 Vitamin D 25-Dihydroxyvitamin D3 TUMOR MICROENVIRONMENT CANCER stem CELL Tumor-infiltrating lymphocyte Tumor-derived endothelial CELL Cancer-associated FIBROBLAST
Extracellular matrix and biomimetic engineering microenvironment for neuronal differentiation 预览
9
作者 Deepak Jain Sabrina Mattiassi +1 位作者 Eyleen L.Goh Evelyn K.F.Yim 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期573-585,共13页
Extracellular matrix(ECM)influences cell differentiation through its structural and biochemical properties.In nervous system,neuronal behavior is influenced by these ECMs structures which are present in a meshwork,fib... Extracellular matrix(ECM)influences cell differentiation through its structural and biochemical properties.In nervous system,neuronal behavior is influenced by these ECMs structures which are present in a meshwork,fibrous,or tubular forms encompassing specific molecular compositions.In addition to contact guidance,ECM composition and structures also exert its effect on neuronal differentiation.This short report reviewed the native ECM structure and composition in central nervous system and peripheral nervous system,and their impact on neural regeneration and neuronal differentiation.Using topographies,stem cells have been differentiated to neurons.Further,focussing on engineered biomimicking topographies,we highlighted the role of anisotropic topographies in stem cell differentiation to neurons and its recent temporal application for efficient neuronal differentiation. 展开更多
关键词 biomimetic platforms biophysical cues contact guidance extracellular matrix neuronal development neural regeneration neural stem cell niche neuronal differentiation neuronal maturation stem cell topography
在线阅读 下载PDF
LSD1 inhibition suppresses the growth of clear cell renal cell carcinoma via upregulating P21signaling
10
作者 Liangsong Zhu Jianfeng Wang +5 位作者 Wen Kong Jiwei Huang Baijun Dong Yiran Huang Wei Xue Jin Zhang 《药学学报:英文版》 CSCD 2019年第2期324-334,共11页
Histone lysine-specific demethylase 1(LSD1) has been implicated in the disease progression of several types of solid tumors. This study provides the first evidence showing that LSD1 overexpression occurred in 62.6%(22... Histone lysine-specific demethylase 1(LSD1) has been implicated in the disease progression of several types of solid tumors. This study provides the first evidence showing that LSD1 overexpression occurred in 62.6%(224/358) of clear cell renal cell carcinomas(ccRCC). LSD1 expression was associated with the progression of ccRCC, as indicated by TNM stage(P ? 0.006), especially tumor stage(P ? 0.017) and lymph node metastasis(P ? 0.030). High LSD1 expression proved to be an independent prognostic factor for poor overall survival(Po0.001) and recurrence-free survival(Po0.001) of ccRCC patients. We further show that LSD1 inhibition by siRNA knockdown or using the small molecule inhibitor SP2509 suppressed the growth of ccRCC in vitro and in vivo. Mechanistically, inhibition of LSD1 decreased the H3 K4 demethylation at the CDKN1 A gene promoter, which was associated with P21 upregulation and cell cycle arrest at G1/S in ccRCC cells. Our findings provide new mechanistic insights into the role of LSD1 in cc RCC and suggest the therapeutic potential of LSD1 inhibitors in ccRCC treatment. 展开更多
关键词 CLEAR CELL RENAL CELL carcinoma LSD1 PROGNOSIS PROLIFERATION P21
miR-34c inhibits proliferation and enhances apoptosis in immature porcine Sertoli cells by targeting the SMAD7 gene 预览
11
作者 RAN Mao-liang WENG Bo +4 位作者 CAO Rong PENG Fu-zhi LUO Hui GAO Hu CHEN Bin 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第2期449-459,共11页
MicroRNAs(miRNAs) are implicated in swine spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation. Recent studies indicated that miR-34 c is indispensable in the late steps of sperm... MicroRNAs(miRNAs) are implicated in swine spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation. Recent studies indicated that miR-34 c is indispensable in the late steps of spermatogenesis. However, whether miR-34 c plays similar important roles in immature porcine Sertoli cells remain unknown. In the present study, we conducted two experiments using a completely randomised design to study the function roles of miR-34 c. The results from experiment I demonstrated that the relative expression level of miR-34 c in swine testicular tissues increased(P=0.0017) quadratically with increasing age, while the relative expression level of SMAD family member 7(SMAD7) decreased(P=0.0009) with curve. Furthermore, miR-34 c expression levels showed a significant negative correlation(P=0.013) with SMAD7 gene expression levels. The results from experiment II indicated that miR-34 c directly targets the SMAD7 gene using a luciferase reporter assay, and suppresses(P<0.05) SMAD7 mRNA and protein expressions in immature porcine Sertoli cells. Overexpression of miR-34 c inhibited(P<0.05) proliferation and enhanced(P<0.05) apoptosis in the immature porcine Sertoli cells, which was supported by the results from the Cell Counting Kit-8(CCK-8) assay, the 5-Ethynyl-2′-deoxyuridine(EdU) assay, and the Annexin V-FITC/PI staining assay. Furthermore, knockdown of SMAD7 via small interfering RNA(siR NA) gave a similar result. It is concluded that miR-34 c inhibits proliferation and enhances apoptosis in immature porcine Sertoli cells by targeting the SMAD7 gene. 展开更多
关键词 IMMATURE PORCINE Sertoli CELL CELL PROLIFERATION APOPTOSIS SMAD7 miR-34c
在线阅读 下载PDF
A novel mouse model of adult T-cell leukemia cell invasion into the spinal cord 预览
12
作者 Takeo Ohsugi Shuhei Tanaka +5 位作者 Keigo Iwasaki Yusuke Nagano Tomohiro Kozako Kazuya Matsuda Takuya Hirose Kazushige Takehana 《动物模型与实验医学(英文)》 CSCD 2019年第1期64-67,共4页
Adult T-cell leukemia(ATL)is a mature T-cell malignancy caused by human T-cell leukemia virus type I infection,and 10%-25%of patients show central nervous system(CNS)involvement.CNS involvement significantly reduces s... Adult T-cell leukemia(ATL)is a mature T-cell malignancy caused by human T-cell leukemia virus type I infection,and 10%-25%of patients show central nervous system(CNS)involvement.CNS involvement significantly reduces survival and there are no effective treatments for CNS involvement.Therefore,an appropriate animal model is required to evaluate the inhibitory effects of novel drugs on the progression of ATL with CNS involvement.Here,we established a mouse model of ATL with CNS involvement using NOD.Cg-Prkdc scid Il2rg tm1Wjl/SzJ mice inoculated with ATL cells intramuscularly in the postauricular region,and these mice showed paraparesis.Of the 10 mice inoculated with ATL cells intramuscularly(I.M.)at 5 weeks of age,8(80%)showed paraparesis,whereas none of the 10 mice inoculated with ATL cells subcutaneously(S.C.)showed paraparesis.In the I.M.group,PCR detected HTLV-1-specific genes in the thoracic and lumbar vertebrae;however,in the S.C.group,the vertebrae were negative for HTLV-1 genes.Histological analysis revealed a particularly high incidence of tumors,characterized by accumulation of the injected cells,in the thoracic vertebrae of mice in the I.M.group.Tumor cell infiltration was relatively high in the bone marrow.Spinal cord compression caused by invasion of the tumor mass outside the pia mater was observed in the thoracic vertebrae of the spinal cord.In conclusion,we have reported a mouse model of tumor growth with paraparesis that may be used to assess novel therapeutic agents for ATL with CNS involvement. 展开更多
关键词 adult T-CELL leukemia(ATL) central nervous system(CNS) human T-CELL LEUKEMIA virus type I(HTLV-1) MICE NOD.Cg-Prkdc SCID Il2rg tm1Wjl/SzJ MICE
在线阅读 免费下载
A case study on the detailed reproducibility of a Human Cell Atlas project
13
作者 Kui Hua Xuegong Zhang 《中国电气与电子工程前沿:英文版》 CSCD 2019年第2期162-169,共8页
Background:Reproducibility is a defining feature of a scientific discovery.Reproducibility can be at different levels for different types of study.The purpose of the Human Cell Atlas (HCA) project is to build maps of ... Background:Reproducibility is a defining feature of a scientific discovery.Reproducibility can be at different levels for different types of study.The purpose of the Human Cell Atlas (HCA) project is to build maps of molecular signatures of all human cell types and states to serve as references for future discoveries.Constructing such a complex reference atlas must involve the assembly and aggregation of data from multiple labs,probably generated with different technologies.It has much higher requirements on reproducibility than individual research projects.To add another layer of complexity,the bioinformatics procedures involved for single-cell data have high flexibility and diversity.There are many factors in the processing and analysis of single-cell RNA-seq data that can shape the final results in different ways.Methods:To study what levels of reproducibility can be reached in current practices,we conducted a detailed reproduction study for a well-documented recent publication on the atlas of human blood dendritic cells as an example to break down the bioinformatics steps and factors that are crucial for the reproducibility at different levels.Results:We found that the major scientific discovery can be well reproduced after some efforts,but there are also some differences in some details that may cause uncertainty in the future reference.This study provides a detailed case observation on the on-going discussions of the type of standards the HCA community should take when releasing data and publications to guarantee the reproducibility and reliability of the future atlas.Conclusion:Current practices of releasing data and publications may not be adequate to guarantee the reproducibility of HCA.We propose building more stringent guidelines and standards on the information that needs to be provided along with publications for projects that evolved in the HCA program. 展开更多
关键词 HUMAN CELL ATLAS REPRODUCIBILITY single CELL BIOINFORMATICS
Hepatic senescence,the good and the bad 预览
14
作者 Nazmul Huda Gang Liu +3 位作者 Honghai Hong Shengmin Yan Bilon Khambu Xiao-Ming Yin 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第34期5069-5081,共13页
Gradual alterations of cell’s physiology and functions due to age or exposure to various stresses lead to the conversion of normal cells to senescent cells.Once becoming senescent,the cell stops dividing permanently ... Gradual alterations of cell’s physiology and functions due to age or exposure to various stresses lead to the conversion of normal cells to senescent cells.Once becoming senescent,the cell stops dividing permanently but remains metabolically active.Cellular senescence does not have a single marker but is characterized mainly by a combination of multiple markers,such as,morphological changes,expression of cell cycle inhibitors,senescence associatedβ-galactosidase activity,and changes in nuclear membrane.When cells in an organ become senescent,the entire organism can be affected.This may occur through the senescence-associated secretory phenotype(SASP).SASP may exert beneficial or harmful effects on the microenvironment of tissues.Research on senescence has become a very exciting field in cell biology since the link between age-related diseases,including cancer,and senescence has been established.The loss of regenerative and homeostatic capacity of the liver over the age is somehow connected to cellular senescence.The major contributors of senescence properties in the liver are hepatocytes and cholangiocytes.Senescent cells in the liver have been implicated in the etiology of chronic liver diseases including cirrhosis and hepatocellular carcinoma and in the interference of liver regeneration.This review summarizes recently reported findings in the understanding of the molecular mechanisms of senescence and its relationship with liver diseases. 展开更多
关键词 SENESCENCE SENESCENCE associated SECRETORY phenotype Hepatocyte CHOLANGIOCYTE HEPATIC stellate CELL CELL cycle ARREST DNA damage
在线阅读 免费下载
Exogenous neural stem cell transplantation for cerebral ischemia 预览
15
作者 Ling-Yi Liao Benson Wui-Man Lau +1 位作者 Dalinda Isabel Sánchez-Vida?a Qiang Gao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1129-1137,共9页
Cerebral ischemic injury is the main manifestation of stroke,and its incidence in stroke patients is 70–80%.Although ischemic stroke can be treated with tissue-type plasminogen activator,its time window of effectiven... Cerebral ischemic injury is the main manifestation of stroke,and its incidence in stroke patients is 70–80%.Although ischemic stroke can be treated with tissue-type plasminogen activator,its time window of effectiveness is narrow.Therefore,the incidence of paralysis,hypoesthesia,aphasia,dysphagia,and cognitive impairment caused by cerebral ischemia is high.Nerve tissue regeneration can promote the recovery of the aforementioned dysfunction.Neural stem cells can participate in the reconstruction of the damaged nervous system and promote the recovery of nervous function during self-repair of damaged brain tissue.Neural stem cell transplantation for ischemic stroke has been a hot topic for more than 10 years.This review discusses the treatment of ischemic stroke with neural stem cells,as well as the mechanisms of their involvement in stroke treatment. 展开更多
关键词 nerve REGENERATION STEM CELL therapy NEURAL STEM cells CELL transplantation ischemic stroke cerebral ischemia NEUROPLASTICITY functional recovery NEURAL REGENERATION
在线阅读 下载PDF
基于MicroStation插件PCS的构件参数化研究 预览
16
作者 贾辉 孙凯强 杜东山 《现代工业经济和信息化》 2019年第6期14-15,17共3页
三维模型是BIM后期应用的基础,使用Microstation软件建模时,部分构件不能参数化建模和重复利用,降低了工作效率。介绍了Microstation插件PCS创建参数化单元的方法,能够重复利用参数单元、并可根据需要设置二维出图符号,很好地弥补软件... 三维模型是BIM后期应用的基础,使用Microstation软件建模时,部分构件不能参数化建模和重复利用,降低了工作效率。介绍了Microstation插件PCS创建参数化单元的方法,能够重复利用参数单元、并可根据需要设置二维出图符号,很好地弥补软件本身单元库的不足,提高了模型质量和工作效率。 展开更多
关键词 BIM MICROSTATION PCS 单元 参数化单元
在线阅读 下载PDF
shRNA-interfering LSD1 inhibits proliferation and invasion of gastric cancer cells via VEGF-C/PI3K/AKT signaling pathway 预览
17
作者 Hong-Ming Pan Wei-Ya Lang +2 位作者 Li-Jie Yao Yan Wang Xiao-Ling Li 《世界胃肠肿瘤学杂志:英文版(电子版)》 CAS 2019年第8期622-633,共12页
BACKGROUND Histone Lysine Specific Demethylase 1(LSD1)is the first histone demethylase to be discovered,which regulates various biological functions by making lysine of histone H3K4,H3K9 and non-histone substrates dem... BACKGROUND Histone Lysine Specific Demethylase 1(LSD1)is the first histone demethylase to be discovered,which regulates various biological functions by making lysine of histone H3K4,H3K9 and non-histone substrates demethylated.Abnormal regulation of LSD1 is closely related to the occurrence and development of gastric cancer.The change of LSD1 expression level plays an important role in the proliferation and metastasis of gastric cancer cells.The study of its function and mechanism may provide a theoretical basis for early diagnosis and targeted therapy of gastric cancer.AIM To investigate the effect of downregulation of lysine-specific demethylase 1(LSD1)expression on proliferation and invasion of gastric cancer cells and the possible regulatory mechanisms of the VEGF-C/PI3K/AKT signaling pathway.METHODS The LSD1-specific short hairpin RNA(shRNA)interference plasmid was transiently transfected,and expression of LSD1 was downregulated.The cell proliferation ability of LSD1 was observed by CCK-8 assay after downregulating expression of LSD1.Transwell invasion assay was used to observe the change of cell invasion ability after downregulating expression of LSD1.Expression of phosphorylated phosphoinositide 3-kinase(p-PI3K),PI3K,p-AKT,AKT,vascular endothelial growth factor receptor(VEGFR)-3,matrix metalloproteinase(MMP)-2 and MMP-9 in each group was detected by Western blotting.RESULTS The cell proliferation ability of transiently transfected LSD1-shRNA interference plasmid group was significantly lower than that of the control group(P<0.05).Transwell invasion assay showed that the number of cells across the membrane of the LSD1-shRNA transfection group(238.451±5.216)was significantly lower than that of the control group(49.268±6.984)(P<0.01).Western blotting showed that expression level of VEGF-C,p-PI3K,PI3K,p-AKT,AKT,VEGFR-3,MMP-2 and MMP-9 in the LSD1-shRNA group was significantly lower than that in the control group(P<0.05).CONCLUSION Downregulation of LSD1 expression inhibits metastatic potential of gastric can 展开更多
关键词 Gastric cancer Lysine specific histone DEMETHYLASE 1 CELL PROLIFERATION CELL INVASION VEGF-C/PI3K/AKT signaling pathway
在线阅读 免费下载
Identification of microRNAs and messenger RNAs involved in human umbilical cord mesenchymal stem cell treatment of ischemic cerebral infarction using integrated bioinformatics analysis 预览
18
作者 Yin-Meng Qu Xin Sun +3 位作者 Xiu-Li Yan Hang Jin Zhen-Ni Guo Yi Yang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1610-1616,共7页
In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are i... In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are involved in various biochemical processes,but the role of microRNAs(miRNAs)in this process is still unclear.From the Gene Expression Omnibus(GEO)database,we downloaded two microarray datasets for GSE78731(messenger RNA(mRNA)profile)and GSE97532(miRNA profile).The differentially expressed genes screened were compared between the hUMSC group and the middle cerebral artery occlusion group.Gene ontology enrichment and pathway enrichment analyses were subsequently conducted using the online Database for Annotation,Visualization,and Integrated Discovery.Identified genes were applied to perform weighted gene co-suppression analyses,to establish a weighted co-expression network model.Furthermore,the protein-protein interaction network for differentially expressed genes from turquoise modules was built using Cytoscape(version 3.40)and the most highly correlated subnetwork was extracted from the protein-protein interaction network using the MCODE plugin.The predicted target genes for differentially expressed miRNAs were also identified using the online database starBase v3.0.A total of 3698 differentially expressed genes were identified.Gene ontology analysis demonstrated that differentially expressed genes that are related to hUMSC treatment of ischemic cerebral infarction are involved in endocytosis and inflammatory responses.We identified 12 differentially expressed miRNAs in middle cerebral artery occlusion rats after hUMSC treatment,and these differentially expressed miRNAs were mainly involved in signaling in inflammatory pathways,such as in the regulation of neutrophil migration.In conclusion,we have identified a number of differentially expressed genes and differentially expressed mRNAs,miRNA-mRNAs,and signaling pathways involved in the hUMSC treatment of ischemic cerebral infarction.Bioinformatics and 展开更多
关键词 nerve REGENERATION ischemic cerebral infarction human umbilical cord mesenchymal STEM CELL TREATMENT bioinformatics analysis DIFFERENTIALLY EXPRESSED genes DIFFERENTIALLY EXPRESSED mRNAs inflammatory response STEM CELL therapy weighted gene co-suppression analysis WGCNA protein-protein interaction network PPI hUMSC neural REGENERATION
在线阅读 下载PDF
Lab-in-cell based on spontaneous amino-yne click polymerization
19
作者 Rong Hu Xu Chen +5 位作者 Taotao Zhou Han Si Benzhao He Ryan T. K. Kwok Anjun Qin Ben Zhong Tang 《中国科学:化学英文版》 SCIE EI CAS CSCD 2019年第9期1198-1203,共6页
Unnatural reaction in the living cells is a powerful tool for biological research. However, the polymerization inside cells is rarely reported. In this work, a lab-in-cell is illustrated based on our developed spontan... Unnatural reaction in the living cells is a powerful tool for biological research. However, the polymerization inside cells is rarely reported. In this work, a lab-in-cell is illustrated based on our developed spontaneous amino-yne click polymerization. Carbonyl group activated terminal diyne can spontaneously polymerize with tetraphenylethene(TPE)-containing primary diamine inside cells, and polymer with weight-average molecular weight of 7,300 was yielded. By utilizing this in vivo amino-yne click polymerization and taking advantage of the aggregation-induced emission feature of TPE, a "turn-on" cell imaging was realized,and in-situ killing of cells was also acquired by destroying the structures of actin and tubulin, which cannot be realized by preprepared polymer. This strategy provides a useful platform and holds great promise in biochemistry and therapy applications. 展开更多
关键词 LAB in CELL INTRACELLULAR POLYMERIZATION aggregation-induced emission CELL imaging
Overexpression of steroid receptor coactivators alleviates hyperglycemia-induced endothelial cell injury in rats through activating the PI3K/Akt pathway
20
作者 Xiao-juan Quan Chun-lian Liang +2 位作者 Ming-zhu Sun Lin Zhang Xiu-li Li 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第5期648-657,共10页
Hyperglycemia is a major factor in vascular endothelial injury that finally leads to a cardiovascular event. Steroid receptor coactivators (SRCs) are a group of non-DNA binding proteins that induce structural changes ... Hyperglycemia is a major factor in vascular endothelial injury that finally leads to a cardiovascular event. Steroid receptor coactivators (SRCs) are a group of non-DNA binding proteins that induce structural changes in steroid receptors (nuclear receptors) critical for transcriptional activation. SRCs, namely, SRC-1, SRC-2, and SRC-3, are implicated in the regulation of vascular homeostasis. In this study we investigate the role of SRCs in hyperglycemia-induced endothelial injury. Aortic endothelial cells were prepared from normal and diabetic rats, respectively. Diabetic rats were prepared by injection of streptozotocin (50 mg/kg, i.p.). The expression levels of SRC-1 and SRC-3 were signi?cantly decreased in endothelial cells from the diabetic rats. Similar phenomenon was also observed in aortic endothelial cells from the normal rats treated with a high glucose (25 mM) for 4 h or 8 h. The expression levels of SRC-2 were little affected by hyperglycemia. Overexpression of SRC-1 and SRC-3 in high glucose-treated endothelial cells significantly increased the cell viability, suspended cell senescence, and inhibited cell apoptosis compared with the control cells. We further showed that overexpression of SRC-1 and SRC-3 markedly suppressed endothelial injury through restoring nitric oxide production, upregulating the expression of antioxidant enzymes (SOD, GPX, and CAT), and activating the PI3K/Akt pathway. The beneficial effects of SRC-1 and SRC-3 overexpression were blocked by treatment with the PI3K inhibitor LY294002 (10 mM) or with the Akt inhibitor MK-2206 (100 nM). In conclusion, hyperglycemia decreased SRC-1 and SRC-3 expression levels in rat aortic endothelial cells. SRC-1 and SRC-3 overexpression might protect against endothelial injury via inhibition of oxidative stress and activation of PI3K/Akt pathway. 展开更多
关键词 DIABETICS STREPTOZOTOCIN HYPERGLYCEMIA endothelial cells STEROID receptor COACTIVATORS CELL senescence CELL apoptosis oxidative stress PI3K/Akt pathway LY294002 MK-2206
上一页 1 2 250 下一页 到第
使用帮助 返回顶部 意见反馈