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不同免疫状态患者念珠菌血症的实验室检测特征分析 预览
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作者 陈盈竹 夏云 《中国感染与化疗杂志》 CAS CSCD 北大核心 2020年第1期49-54,共6页
目的探讨不同免疫状态患者念珠菌血症的病原菌分布及药敏、血清标志物等实验室检验指标的差异。方法回顾性收集分析2016年1月-2018年12月重庆医科大学附属第一医院念珠菌血症患者的临床和实验室相关资料。结果103例患者纳入分析,免疫抑... 目的探讨不同免疫状态患者念珠菌血症的病原菌分布及药敏、血清标志物等实验室检验指标的差异。方法回顾性收集分析2016年1月-2018年12月重庆医科大学附属第一医院念珠菌血症患者的临床和实验室相关资料。结果103例患者纳入分析,免疫抑制组58例,非免疫抑制组45例。非免疫抑制组接受体内导管置入和完全肠外营养的比例明显高于免疫抑制组;微生物学特征显示免疫抑制组的热带念珠菌分离率明显高于非免疫抑制组,非免疫抑制组的近平滑念珠菌分离率明显高于免疫抑制组;对三唑类抗真菌药物的整体敏感率免疫抑制组低于非免疫抑制组,耐药菌主要为热带念珠菌;发生念珠菌血症时,两组患者血清降钙素原和C反应蛋白值均高于参考值范围;免疫抑制组患者血清(1,3)-β-D葡聚糖(BDG)阴性率明显高于非免疫抑制组。结论根据患者不同免疫状态,更好识别不同患者的病原菌分布特点,有助于临床医师选择更合理的抗真菌药物。然而,血清BDG检测不能早期识别所有念珠菌血症患者,特别是免疫抑制患者,需要结合降钙素原和C反应蛋白值综合判断。 展开更多
关键词 念珠菌 免疫抑制 非免疫抑制 血流感染
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Comparative Study of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Culture Test for <i>Candida</i>Identification 预览
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作者 Yoriko Kubota Chieko Taguchi +7 位作者 Masanori Saito Noriko Shinozaki-Kuwahara Tomoko Suzuki Masaaki Suemitsu Mitsuko Nakayama Tadahiko Utsunomiya Hiroyasu Endo Kayo Kuyama 《口腔学期刊(英文)》 2019年第12期295-306,共12页
Background: A new microorganism identification method using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) has been developed, however, reports on its use for delineating Candida spp. are... Background: A new microorganism identification method using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) has been developed, however, reports on its use for delineating Candida spp. are scarce. Objectives: The purpose of this study was to compare the identification accuracy of mixed infection between culture test and MALDI-TOF MS. Materials and Methods: Eighty-nine denture wearers (average 74.0 ± 9 years) were selected. Specimens were immediately inoculated onto selective medium for CHROMagarTM Candida, and were also carried out using MALDI-TOF MS. The distribution frequencies of them were analyzed. Results: The numbers and rates of detection/non-detection by MALDI-TOF MS of genus Candida were 58/31 (65.2%/34.8%), respectively. Infection types were single infection in 34 (38.2%), mixed infection in 24 (27.0%), and non-infection in 31 (34.8%) cases. Concerning the single infection, C. albicans was the most predominant (58.8%), followed by C. parapsilosis (17.6%), C. glabrata (14.7%), C. tropicalis (5.9%), and C. krusei (2.9%). As for the mixed infection, the most frequent combination was C. albicans and C. glabrata (50.0%), followed by C. albicans and C. parapsilosis (29.2%), C. albicans and C. tropicalis (8.3%), C. glabrata and C. tropicalis (4.2%), C. albicans, C. glabrata, and C. parapsilosis (4.2%), and C. albicans, C. parapsilosis, and C. glabrata (4.2%). There were four MALDI-TOF MS positive results that were negative by the culture test. Conversely, there were six MALDI-TOF MS negative results that were positive by the culture test. The sensitivity and specificity of MALDI-TOF MS were 0.929 and 0.840, respectively. The concordance rate of genus Candida was 0.644, indicating substantial agreement. Conclusion: Candida infection is complicated by disease type and oral cavity environment changes due to aging. A rapid microorganism detection method, such as MALDI-TOF MS, will be helpful to quickly determine the causative pathogen in dental infections. 展开更多
关键词 CANDIDA CULTURE TEST MALDI-TOF MS Non-C. ALBICANS CANDIDA (NCAC)
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Postoperative Endophthalmitis Caused by Candida Parapsilosis: A Case Report 预览
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作者 María del Pilar Lucena Federico Furno Sola +1 位作者 Mitzy E. Torres Soriano Fanny Andrea Gerster 《眼科学期刊(英文)》 2019年第2期64-69,共6页
Purpose: To report an unusual case of postcataract endophthalmitis secondary to Candida parapsilosis. Case Presentation: A 77-year-old female patient was admitted to the emergency ward with reduced visual acuity (VA),... Purpose: To report an unusual case of postcataract endophthalmitis secondary to Candida parapsilosis. Case Presentation: A 77-year-old female patient was admitted to the emergency ward with reduced visual acuity (VA), and redness and pain in the right eye (RE) with a few hours of evolution. The patient underwent cataract surgery in the right eye 50 days earlier;no abnormalities were discovered in postsurgery check-ups on the following days. Biomicroscopy revealed corneal oedema, tyndall ++++, and flare +++. Capsule-shaped whitish deposits and posterior synechiae were also reported. Fundoscopy: not visible due to anterior segment opacity. RE ultrasound: mild vitritis, attached retina. Due to a strong suspicion of endophthalmitis, an aqueous humour biopsy was performed and the presence of Candida parapsilosis sensitive to amphotericin B and voriconazole was revealed. In view of a poor prognosis, we decided to carry out a vitrectomy, removal of the IOL + capsular bag complex and intravitreal injection of amphotericin B. After several check-ups, the patient reported being diagnosed with a fungal infection in her fingernails some time ago. She was referred for a microbiological testing, which confirmed the presence of Candida in the fingernails. Conclusions: Fungal endophthalmitis is uncommon in the postoperative setting, with most of the cases being attributable to the Candida species. The combination of pars plana vitrectomy, removal of the IOL + capsular bag complex and antifungal agents was able to successfully treat this case of postoperative endophthalmitis due to Candida parapsilosis. The relationship between postoperative Candida endophthalmitis and Candida nail infection is dramatic. To date, only one case of endogenous endophthalmitis associated with complicated onychomycosis has been reported. 展开更多
关键词 ENDOPHTHALMITIS CANDIDA CATARACT Surgery NAIL INFECTION
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HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth(Lamiaceae) against Candida strains
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作者 Janaína Esmeraldo Rocha Henrique Douglas Melo Coutinho +7 位作者 Cícero Roberto Nascimento Saraiva Antonia Thassya Lucas dos Santos Antonio Judson Targino Machado Joao Tavares Calixto Júnior Irwin Rose Alencar Menezes JoséGalberto Martins da Costa Jaime Ribeiro-Filho Aracelio Viana Colares 《亚太热带生物医学杂志:英文版》 CAS 2019年第3期123-128,共6页
Objective: To evaluate the anti Candida activity of Hyptis martiusii decoction and its major compound, caffeic acid alone or in the presence of fluconazole, as well as their cytotoxic effect. Methods: The decoction wa... Objective: To evaluate the anti Candida activity of Hyptis martiusii decoction and its major compound, caffeic acid alone or in the presence of fluconazole, as well as their cytotoxic effect. Methods: The decoction was characterized using high performance liquid chromatography coupled with diode array detector. For the antifungal activity, the minimum inhibitory concentration(MIC) and the potential effect of the decoction with the fluconazole were evaluated by microdilution method using 96-well microtiter trays. The osmotic fragility test was performed using erythrocytes under saline stress. All tests were performed in triplicate. Results: The chemical characterization of the decoction was performed by high performance liquid chromatography and revealed the presence of seven compounds, including caffeic acid as major constituent. The antifungal tests demonstrated that both decoction(DHm) and caffeic acid obtained from Hyptis martiusii presented MIC and MFC ≥4096 μg/mL against Candida albicans and Candida tropicalis strains. However, in the presence of fluconazole, DHm and caffeic acid presented IC50 of 2.60 and 2.53 μg/mL respectively, demonstrating significant synergistic effects against Candida strains. The modulator activity of DHm might be due to the presence of caffeic acid. Moreover, DHm and caffeic acid did not cause significant hemolytic effects, indicating that they present low cytotoxicity. Conclusions: These data indicate that DHm potentiates the activity of the fluconazole, without enhancement of the toxicity, encouraging further toxicological, pharmacological and phytochemical studies to provide consistent evidence of the potential of this plant to be used in drug development. 展开更多
关键词 Hyptis martiusii Caffeic acid CANDIDA ALBICANS CANDIDA TROPICALIS ANTIFUNGAL properties
Optimization of Candida tropicalis Fermentation Conditions to Produce Long-chain Dibasic Acids 预览
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作者 Xiang XIU Junqing WANG 《农业生物技术:英文版》 CAS 2019年第2期160-164,共5页
[Objectives] The fermentation conditions of Candida tropicalis 1798- pxa 1 were optimized to further improve its yield of long-chain dibasic acids.[Methods] The strains used in the study were C. tropicalis 1798, C. tr... [Objectives] The fermentation conditions of Candida tropicalis 1798- pxa 1 were optimized to further improve its yield of long-chain dibasic acids.[Methods] The strains used in the study were C. tropicalis 1798, C. tropicalis 1798- pxa 1 and C. tropicalis 1798- pxa1p2 . First, through single factor experiments, the activated three fungi were cultured in different carbon sources, and the absorbance was measured every 2h. The growth curve was drawn by software, and finally the most suitable substance for the substrate was selected, i.e., dodecane. Then, the composition of the fermentation medium and the fermentation process parameters were determined through the PB experiment of the single-deletion C. tropicalis 1798- pxa1 .[Results] The experiment determined the important components affecting the synthesis of long-chain dibasic acids, namely (NH4)2SO4, NaCl and dodecane. After optimization of the culture conditions, the yield of long-chain dibasic acids of C. tropicalis 1798- pxa1 increased from 5.6 to 10.1 g/L.[Conclusions] The scheme has been verified to be capable of greatly increasing the yield of the corresponding long-chain dibasic acids of the C. tropicalis engineering strain. 展开更多
关键词 Synthesis of LONG-CHAIN dibasic acid CANDIDA TROPICALIS engineering FUNGUS Single factor EXPERIMENT PB EXPERIMENT Condition OPTIMIZATION
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孕妇阴道假丝酵母菌感染危险因素研究
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作者 夏丽 张崇星 《智慧健康》 2019年第5期36-38,共3页
目的探讨孕妇阴道假丝酵母菌感染的相关危险因素,指导临床防治孕妇阴道假丝酵母菌感染。方法选取2015年9月至2017年9月于新泰市人民医院就诊的孕妇1628例,收集每个病例临床资料。采用单因素及多因素Logistic回归模型,分析阴道假丝酵母... 目的探讨孕妇阴道假丝酵母菌感染的相关危险因素,指导临床防治孕妇阴道假丝酵母菌感染。方法选取2015年9月至2017年9月于新泰市人民医院就诊的孕妇1628例,收集每个病例临床资料。采用单因素及多因素Logistic回归模型,分析阴道假丝酵母菌感染的相关危险因素。结果 1628例孕妇阴道假丝酵母菌感染率为37.53%。多因素Logistic回归分析显示,孕前体重指数(OR=1.235,95%CI:1.057~1.443)、流产史(OR=1.848,95%CI:1.159~2.946)、孕前外阴阴道假丝酵母菌病(VVC)史(OR=3.725,95%CI:1.680~8.256)、孕期情绪焦虑(OR=2.085,95%CI:1.181~3.686)及妊娠合并糖尿病(OR=2.586,95%CI:1.334~5.011)均是孕妇阴道假丝酵母菌感染的独立危险因素(P<0.05)。结论孕前体重指数、流产史、孕前VVC史、孕期情绪焦虑及妊娠合并糖尿病这5项是孕妇阴道假丝酵母菌感染的独立危险因素,可为临床防治孕妇阴道假丝酵母菌感染提供指导。 展开更多
关键词 孕妇 假丝酵母菌 感染 危险因素
儿科重症监护病房院内侵袭性真菌血流感染21例临床分析
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作者 刘波 王丽杰 《中国实用儿科杂志》 CSCD 北大核心 2019年第6期508-512,共5页
目的提高对侵袭性真菌感染(invasive fungal infection,IFI)的认识,帮助早期诊断、早期治疗,积极改善预后。方法选取2012年1月至2018年1月于中国医科大学附属盛京医院儿科重症监护病房(PICU)住院,临床诊断IFI血流感染的21例患儿为研究对... 目的提高对侵袭性真菌感染(invasive fungal infection,IFI)的认识,帮助早期诊断、早期治疗,积极改善预后。方法选取2012年1月至2018年1月于中国医科大学附属盛京医院儿科重症监护病房(PICU)住院,临床诊断IFI血流感染的21例患儿为研究对象,回顾性分析其临床特点、高危因素及预后。结果临床诊断IFI血流感染的21例患儿中,真菌感染的发生与年龄性别无明显相关性,致病菌以白色念珠菌及近平滑假丝酵母菌为主,近平滑假丝酵母菌病死率较高(80%);21例真菌血流感染的患儿,28d随访存活8例,死亡13例,病死率高达61.90%;原发病以呼吸系统(14.29%)、消化系统疾病(23.81%)居多,其中重症肺炎4例,消化道穿孔4例;高危因素主要以广谱抗生素联合应用、侵入性操作(气管插管、中心静脉置管、导尿及留置胃管等)、长期ICU住院等为著,均在85%以上。真菌感染多表现为不典型发热(80.95%),白细胞异常升高或降低(47.62%),C反应蛋白多升高(80.95%),G试验的阳性率可达到42.86%;存活组的8例患儿诊断真菌感染前75%预防性应用抗真菌治疗,死亡组预防用药率为53.85%。结论长期ICU住院、大量广谱抗生素的长期及联合应用、侵入性操作、自身免疫缺陷等因素的存在增加了真菌感染的风险,对于临床原发病基础上出现不典型难治感染,要警惕真菌感染,应积极完善相关微生物学检查。 展开更多
关键词 侵袭性真菌感染 念珠菌 高危因素 临床分析
小檗碱对5种临床常见念珠菌体外抑菌作用分析 预览
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作者 姚冬婷 胡骏 +2 位作者 张雪清 张俊 胡晓波 《检验医学》 CAS 2019年第7期587-590,共4页
目的检测小檗碱对5种临床常见念珠菌的抑菌效果。方法收集分离自临床样本的56株念株菌(白念珠菌23株、光滑念珠菌20株、热带念珠菌6株、克柔念珠菌4株、近平滑念珠菌3株),采用微量肉汤稀释法对其进行体外药物敏感性试验。结果小檗碱对... 目的检测小檗碱对5种临床常见念珠菌的抑菌效果。方法收集分离自临床样本的56株念株菌(白念珠菌23株、光滑念珠菌20株、热带念珠菌6株、克柔念珠菌4株、近平滑念珠菌3株),采用微量肉汤稀释法对其进行体外药物敏感性试验。结果小檗碱对白念珠菌、光滑念珠菌、热带念珠菌、克柔念珠菌及近平滑念珠菌的最小抑菌浓度(MIC)50分别为32、16、8、4和16μg/mL,对白念珠菌的抑菌效果与其他4种念珠菌比较差异均有统计学意义(P<0.05),小檗碱对光滑念珠菌、热带念珠菌、克柔念珠菌的抑菌效果比较差异无统计学意义(P>0.05),小檗碱对敏感及非敏感光滑念珠菌的抑菌效果差异亦无统计学意义(P=0.063)。结论小檗碱对临床常见的5种念珠菌(包括非敏感光滑念珠菌)均有较好的抑菌效果,对非白念珠菌的抑菌效果优于白念珠菌。 展开更多
关键词 念珠菌 小檗碱 抑菌作用 最小抑菌浓度
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妊娠期外阴阴道假丝酵母菌病发病机制的研究概述
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作者 张仕茜 杨旭 《中国微生态学杂志》 CAS CSCD 2019年第5期617-620,共4页
外阴阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)是由假丝酵母菌引起的常见外阴阴道炎症。VVC在妊娠期有较高的发病率。妊娠期VVC的常见病原体主要是白色假丝酵母菌,其发病机制包括菌体本身的致病因素、妊娠期激素的升高及免疫功... 外阴阴道假丝酵母菌病(vulvovaginal candidiasis,VVC)是由假丝酵母菌引起的常见外阴阴道炎症。VVC在妊娠期有较高的发病率。妊娠期VVC的常见病原体主要是白色假丝酵母菌,其发病机制包括菌体本身的致病因素、妊娠期激素的升高及免疫功能下降。菌体的致病因素包括粘附力、细胞外酶及形态转换。同时VVC对妊娠有一定影响,主要导致宫内感染、胎膜早破、早产、低体质量儿出生、流产、死胎、假丝酵母菌性肺炎等疾病。本文就国内外妊娠期外阴阴道假丝酵母菌病的发病机制进行综述。 展开更多
关键词 妊娠期 假丝酵母菌 外阴阴道炎
卡泊芬净对16种念珠菌体外抗菌活性研究 预览
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作者 张丽 于淑颖 +5 位作者 李颖 王贺 肖盟 张戈 张京家 徐英春 《中国感染与化疗杂志》 CAS CSCD 北大核心 2019年第5期530-536,共7页
目的比较卡泊芬净国产与进口制剂对引起侵袭性感染的念珠菌属体外抗菌活性。方法从29所医院收集了分离自血液等无菌部位的337株念珠菌,包括白念珠菌、热带念珠菌、近平滑念珠菌、光滑念珠菌、克柔念珠菌等16种念珠菌,采用CLSI推荐的微... 目的比较卡泊芬净国产与进口制剂对引起侵袭性感染的念珠菌属体外抗菌活性。方法从29所医院收集了分离自血液等无菌部位的337株念珠菌,包括白念珠菌、热带念珠菌、近平滑念珠菌、光滑念珠菌、克柔念珠菌等16种念珠菌,采用CLSI推荐的微量肉汤稀释法对8种抗真菌药物进行体外药物敏感性试验。结果除1株光滑念珠菌和1株克柔念珠菌对国产卡泊芬净中介外,其余菌株均敏感。不同菌种药敏数据显示,国产与进口卡泊芬净的MIC50和MIC90完全一致,除热带念珠菌外,国产与进口卡泊芬净对其余菌种的MIC分布范围均一致。国产卡泊芬净对常见菌种的MIC50和MIC90为白念珠菌0.064mg/L和0.125mg/L、热带念珠菌0.125mg/L和0.25mg/L、光滑念珠菌0.125mg/L和0.125mg/L、克柔念珠菌0.25mg/L和0.25mg/L,近平滑念珠菌复合体和季也蒙念珠菌的MIC50和MIC90为0.5mg/L和1mg/L,略高于上述念珠菌。分别分析氟康唑敏感和耐药的白念珠菌和热带念珠菌对棘白菌素类药物的敏感率,发现氟康唑耐药组与敏感组菌株对应的棘白菌素类药物MIC分布范围基本一致,几何均数无明显差异,并且均未发现卡泊芬净耐药菌株。结论卡泊芬净对多数念珠菌属均有较好的抗真菌活性,并且国产与进口卡泊芬净体外抗真菌活性高度一致。 展开更多
关键词 卡泊芬净 氟康唑 药物敏感性试验 念珠菌
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念珠菌检验在妇科炎症感染中的应用价值分析 预览
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作者 黄敏 《中国继续医学教育》 2019年第27期53-55,共3页
目的总结并归纳念珠菌检验在妇科炎症感染中的应用价值。方法以128例妇科念珠菌感染患者作为研究对象进行此次研究,纳入的患者均于2018年1月—2019年1月期间在本院进行治疗,分别采用镜检法、培养法以及凝集法对患者的阴道分泌物进行念... 目的总结并归纳念珠菌检验在妇科炎症感染中的应用价值。方法以128例妇科念珠菌感染患者作为研究对象进行此次研究,纳入的患者均于2018年1月—2019年1月期间在本院进行治疗,分别采用镜检法、培养法以及凝集法对患者的阴道分泌物进行念珠菌检验,以最终病理结果为金标准,探究念珠菌的检出率,总结不同检验方法对妇科炎症感染患者的诊断有效率。结果镜检法、培养法以及凝集法对128例妇科炎症感染患者进行检查,念珠菌的检出率分别为75.8%(97/128)、78.1%(100/128)、78.9%(101/128),三种方法的检出率相比均无较大差异,数据经统计软件分析体现为P>0.05,差异无统计学意义。结论镜检法、培养法以及凝集法在对妇科炎症感染患者进行分泌物念珠菌检验时均可取得较好的检验结果,为临床治疗提供有效的诊断依据。 展开更多
关键词 妇科 炎症感染 镜检法 培养法 凝集法 念珠菌
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Non-albicans Candida prosthetic joint infections: A systematic review of treatment 预览
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作者 Christos Koutserimpas Stylianos G Zervakis +4 位作者 Sofia Maraki Kalliopi Alpantaki Argyrios Ioannidis Diamantis P Kofteridis George Samonis 《世界临床病例杂志》 2019年第12期1430-1443,共14页
BACKGROUND Non-albicans Candida prosthetic joint infections (PJIs) are rare. Optimal treatment involves a two-stage revision surgery in combination with an antifungal agent. However, no clear guidelines have been deve... BACKGROUND Non-albicans Candida prosthetic joint infections (PJIs) are rare. Optimal treatment involves a two-stage revision surgery in combination with an antifungal agent. However, no clear guidelines have been developed regarding the agent or treatment duration. Hence, a broad range of antifungal and surgical treatments have been reported so far. AIM To clarify treatment of non-albicans Candida PJIs. METHODS A literature review of all existing non-albicans Candida PJIs cases through April 2018 was conducted. Information was extracted about demographics, comorbidities, responsible species, duration and type of antifungal treatment, type of surgical treatment, time between initial arthroplasty and symptom onset, time between symptom onset and definite diagnosis, outcome of the infection and follow-up. RESULTS A total of 83 cases, with a mean age of 66.3 years, were located. The causative yeast isolated in most cases was C. parapsilosis (45 cases;54.2%), followed by C. glabrata (18 cases;21.7%). The mean Charlson comorbidity index was 4.4 ± 1.5. The mean time from arthropalsty to symptom onset was 27.2 ± 43 mo, while the mean time from symptom onset to culture-confirmed diagnosis was 7.5 ± 12.5 mo. A two stage revision arthroplasty (TSRA), when compared to one stage revision arthroplasty, had a higher success rate (96% vs 73%, P = 0.023). Fluconazole was the preferred antifungal agent (59;71%), followed by amphotericin B (41;49.4%). CONCLUSION The combination of TSRA and administration of prolonged antifungal therapy after initial resection arthroplasty is suggested on the basis of limited data. 展开更多
关键词 Fungal PROSTHETIC JOINT INFECTION Knee ARTHROPLASTY INFECTION Hip ARTHROPLASTY INFECTION ANTIFUNGAL TREATMENT Non-albicans Candida PROSTHETIC JOINT infections
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天疱疮患者感染危险因素分析
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作者 刘琼 许贵霞 张思平 《中国皮肤性病学杂志》 CAS CSCD 北大核心 2019年第7期788-791,共4页
目的探讨天疱疮患者感染的危险因素。方法回顾性分析2012-2017年本科58例住院的天疱疮患者的临床特点、感染部位、病原体,分析可能的易感因素。结果有39例发生感染,感染率67.24%;单因素分析显示寻常型天疱疮、口腔受累、病情重、合并糖... 目的探讨天疱疮患者感染的危险因素。方法回顾性分析2012-2017年本科58例住院的天疱疮患者的临床特点、感染部位、病原体,分析可能的易感因素。结果有39例发生感染,感染率67.24%;单因素分析显示寻常型天疱疮、口腔受累、病情重、合并糖尿病、低蛋白血症的患者感染率更高,差异有统计学意义(P<0.05);病情重、合并糖尿病的患者院感率也更高,差异有统计学意义(P<0.05);单独糖皮质激素和糖皮质激素联合环磷酰胺治疗的患者感染率和院感率差异均无统计学意义(P>0.05)。多因素分析显示口腔受累(OR=12.373)、病情重(OR=11.300)、合并糖尿病(OR=17.268)、低蛋白血症(OR=8.699)是感染的独立危险因素;病情重(OR=8.390)、合并糖尿病(OR=4.109)也是院感的独立危险因素。结论天疱疮患者病情严重程度、合并糖尿病、低蛋白血症、口腔黏膜损害是感染的危险因素,环磷酰胺的应用没有增加感染的风险。 展开更多
关键词 天疱疮 感染 糖尿病 低蛋白血症 念珠菌
The general transcriptional repressor Tup1 governs filamentous development in Candida tropicalis
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作者 Jiao Gong Qian Huang +2 位作者 Weihong Liang Yujia Wei Guanghua Huang 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第5期463-470,共8页
Filamentous development is associated with the ability to cause infections and colonize the host in pathogenic Candida species.Candida tropicalis is one of the major fungal pathogens of humans.The conserved transcript... Filamentous development is associated with the ability to cause infections and colonize the host in pathogenic Candida species.Candida tropicalis is one of the major fungal pathogens of humans.The conserved transcriptional repressor Tup1 plays a critical role in the regulation of transcription and filamentation in yeast species.Despite its central role,the full coding sequence of TUP1 has not been found in the reported genome sequence of C.tropicalis to date.In this study,we report the identification of Tup1 and characterize its role in filamentous growth in C.tropicalis.As expected,C.tropicalis Tup1 exhibits general conserved features to the orthologs of other fungi in terms of its structure and function.Deletion of TUP1 in C.tropicalis leads to increased filamentation under several culture conditions.However,Tup1 indeed exhibits species-specific roles in the regulation of filamentous development in C.tropicalis.For example,unlike the tup1/tup1 mutant of Candida albicans,the tup1/tup1 mutant of C.tropicalis is able to exist in the yeast form at low temperatures or in the presence of N-acetylglucosamine(GlcNAc).Acidic pH conditions also favor the yeast form of the tup1/tup1 mutant of C.tropicalis.Quantitative real-time PCR(qRT-PCR)assays indicate that Tup1 may regulate filamentous development through the transcriptional control of key filamentation regulators in C.tropicalis,such as Ume6,Brg1,Wor1,Sfl2,Ahr1,and Zcf3.Taken together,our findings demonstrate both conserved and species-specific roles of Tup1 in the regulation of filamentation and provide novel insights into the biology of C.tropicalis. 展开更多
关键词 CANDIDA TROPICALIS Tup1 FILAMENTATION MORPHOLOGICAL TRANSITIONS
上海地区临床分离念珠菌的耐药性监测 预览
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作者 慎慧 郭建 +29 位作者 张旻 孔雯娜 肖晶晶 吴文娟 刘云 陈险峰 谢潋滟 汤荣 董郭平 李莉 孙燕 李敏 徐阳飏 张静华 黄声雷 奚卫 蔡金凤 陈祝俊 李丽 秦惠宏 舒文 曹文俊 王爱敏 卫颖珏 黄卫春 张雯雁 赵虎 袁轶群 沈振华 房华 《中国感染与化疗杂志》 CAS CSCD 北大核心 2019年第3期292-299,共8页
目的了解上海地区临床分离念珠菌的菌株分布以及对常用抗真菌药物的敏感性和耐药性。方法对上海地区27所医院临床分离念珠菌采用微量液基稀释法按统一方案进行抗真菌药物敏感性试验。按CLSI M60(2017年)判断结果。结果收集2017年8月1日-... 目的了解上海地区临床分离念珠菌的菌株分布以及对常用抗真菌药物的敏感性和耐药性。方法对上海地区27所医院临床分离念珠菌采用微量液基稀释法按统一方案进行抗真菌药物敏感性试验。按CLSI M60(2017年)判断结果。结果收集2017年8月1日-2018年4月30日上述医院临床分离念珠菌共354株,其中白念珠菌占41.81%(148/354),近平滑念珠菌占23.73%(84/354),热带念珠菌占15.54%(55/354),光滑念珠菌占9.32%(33/354),其他念珠菌占9.60%(34/354)。白念珠菌、近平滑念珠菌、光滑念珠菌耐药率与以往报道比较偏低或基本持平,无增长趋势,主要是对唑类抗真菌药物具有一定的耐药性。白念珠菌对氟康唑耐药率为1.45%、剂量依赖性敏感(SDD)率为4.35%,对伏立康唑耐药率和中介率均为2.17%;近平滑念珠菌对氟康唑呈现1.20%的SDD率,对伏立康唑呈现1.20%的中介率;光滑念珠菌对氟康唑表现出6.06%的耐药率和93.94%的SDD率;热带念珠菌耐药率呈增长趋势,对氟康唑表现出41.82%的耐药率和12.73%的SDD率,对伏立康唑表现出40.00%的耐药率和12.73%的中介率。白念珠菌、近平滑念珠菌、热带念珠菌对棘白菌素类药物尚未出现耐药菌株,仅1株光滑念珠菌对卡泊芬净耐药。结论上海地区临床念珠菌的分离率及菌株分布与国内外报道基本一致,临床分离菌株对常用抗菌药物的耐药率基本稳定,热带念珠菌对唑类抗真菌药物的耐药率呈上升趋势,应该引起临床医师重视。同时应进一步加强医院感染防控措施,继续做好真菌耐药性监测工作。 展开更多
关键词 真菌耐药性监测 药物敏感性试验 念珠菌 侵袭性真菌感染
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Etest方法与微量肉汤稀释法检测念珠菌属对唑类抗真菌药物的敏感性评价 预览
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作者 张丽 王贺 +1 位作者 肖盟 徐英春 《现代检验医学杂志》 CAS 2019年第4期67-70,共4页
目的比较分析Etest法检测临床常见念珠菌对氟康唑、伏立康唑和伊曲康唑敏感性与微量肉汤稀释法的一致率。方法针对220株临床常见的5种念珠菌,分别采用CLSI M27微量肉汤稀释法和Etest法进行药物敏感性检测。分别统计Etest法与微量肉汤稀... 目的比较分析Etest法检测临床常见念珠菌对氟康唑、伏立康唑和伊曲康唑敏感性与微量肉汤稀释法的一致率。方法针对220株临床常见的5种念珠菌,分别采用CLSI M27微量肉汤稀释法和Etest法进行药物敏感性检测。分别统计Etest法与微量肉汤稀释法检测三种唑类药物的基本一致率(EA)和分类一致率(CA)。结果 Etest方法和微量肉汤稀释法的EA分别为氟康唑(96.8%)、伏立康唑(97.3%)和伊曲康唑(81.8%)。对于氟康唑、伏立康唑和伊曲康唑,白念珠菌、热带念珠菌、近平滑念珠菌、光滑念珠菌和克柔念珠菌中两种检测方法的CA分别为96.2%,97.5%和—;92.5%,82.5%和97.5%;92.5%,97.5%和—;97.5%,—和70%;—,100%和90%。结论 Etest法检测念珠菌对唑类药物敏感性与微量肉汤稀释法一致率较高,是念珠菌唑类药物敏感性测定简便并且准确性较高的检测方法,推荐在真菌实验室常规使用。 展开更多
关键词 念珠菌 ETEST 微量肉汤稀释法 唑类药物
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Characterization of oral yeasts isolated from healthy individuals attended in different Colombian dental clinics 预览
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作者 Raul Eduardo Rivera Alejandra Zuluaga +18 位作者 Karen Arango Itzjak Kadar Paola Andrea Pinillos Luis Fernando Montes Eugenia Catalina Cepeda Ernesto Gonzalez Pedro Antonio Alfonso Andrea Alejandra Villalba Luis Fernando Casanova Adolfo Perez Armando Roa Martha Jhoana Arias Jorge Orlando Francisco Cuellar Lorena Pedraza Adiel Alberto Vasquez Blanca Lynne Suarez Beatriz LGomez Catalina De Bedout Luz Elena Cano 《生物医学研究杂志:英文版》 CAS CSCD 2019年第5期333-342,共10页
The aim of this study was to identify the most frequent yeasts in the oral cavity of adult individuals without immune disorders and to associate the presence of these oral yeasts with different characteristics of each... The aim of this study was to identify the most frequent yeasts in the oral cavity of adult individuals without immune disorders and to associate the presence of these oral yeasts with different characteristics of each individual. Oral rinse samples were obtained from 96 healthy adults and cultured in Sabouraud dextrose agar media and CHROMagar. Yeasts were identified by sequencing the D1/D2 region of the 28 S rRNA gene. Probable association among the socio-demographic characteristics, body mass index, family and personal medical history,oral hygiene, tobacco and/or alcohol consumption habits and presence of oral fungi was analyzed. Contingency tables and logistic regression were employed to evaluate possible relationships between the presence of oral fungi and mixed colonization with these variables. 57.3% of the healthy individuals had oral yeasts and 21.8% had mixed colonization. The most prevalent yeasts were Candida albicans(52%), C. parapsilosis(17.9%), and C.dubliniensis(7.57%). Yeasts with most frequently mixed colonization were C. albicans and C. parapsilosis. No relationships were found among the variables analyzed. However, the presence of mixed colonization was related to the presence of dental prostheses(P<0.006), dental apparatuses(P=0.016) and O’Leary index(P=0.012). This is the first study that characterized oral yeasts in Colombian healthy individuals, determined the most prevalent oral yeasts C. albicans, C. parapsilosis and C. dublinensis and an association of mixed colonization with the use of dental prostheses and aparatology and poor hygiene. 展开更多
关键词 ORAL YEAST CANDIDA species MICROBIAL EPIDEMIOLOGY
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氯己定联合抗真菌药物抗念珠菌生物膜效应
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作者 刘朝阳 唐璟 +2 位作者 杨宏宇 曾同祥 孙毅 《临床口腔医学杂志》 2019年第9期519-521,共3页
目的:比较氯己定与氟康唑、伊曲康唑、克霉唑联合体外抗念珠菌生物膜效应。方法:利用改良96孔板构建念珠菌生物膜,并参考美国临床实验室标准化研究所(clinical laboratory standard institute,CLSI)M27-A3方案及棋盘法检测氯己定与氟康... 目的:比较氯己定与氟康唑、伊曲康唑、克霉唑联合体外抗念珠菌生物膜效应。方法:利用改良96孔板构建念珠菌生物膜,并参考美国临床实验室标准化研究所(clinical laboratory standard institute,CLSI)M27-A3方案及棋盘法检测氯己定与氟康唑、伊曲康唑和克霉唑对念珠菌生物膜的联合抗菌效应。结果:氯己定、氟康唑、伊曲康唑、克霉唑对念珠菌生物膜的80%最低抑菌浓度SMIC80均>32μg/mL。读取SMIC50时,氯己定与3种抗真菌药物的协同率为100%。读取SMIC80时,氯己定与3种抗真菌药物的协同率均≥80%。结论:在体外,氯己定与3种抗真菌药物联合对念珠菌生物膜时,能够降低3种药物对念珠菌生物膜的SMIC值,增强的抗念珠菌生物膜的作用。 展开更多
关键词 氯己定 念珠菌 生物膜
血液病免疫抑制治疗后播散性白色念珠菌感染的CT表现 预览
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作者 包云飞 翁卿吉 +2 位作者 胡苗苗 赵红星 郑银元 《医学影像学杂志》 2019年第5期808-810,813共4页
目的探讨血液病免疫抑制治疗后的播散性白色念珠菌感染的CT表现。方法分析5例经临床和病理活检证实的免疫抑制治疗后播散性白色念珠菌感染的患者并进行文献复习。结果5例均发现肝多发结节病灶,其中2例伴肺部多发结节病灶,1例伴有脾脏及... 目的探讨血液病免疫抑制治疗后的播散性白色念珠菌感染的CT表现。方法分析5例经临床和病理活检证实的免疫抑制治疗后播散性白色念珠菌感染的患者并进行文献复习。结果5例均发现肝多发结节病灶,其中2例伴肺部多发结节病灶,1例伴有脾脏及双肾、后腹膜区多发结节病灶。肝脏CT平扫均表现为多发类圆形等或略低密度结节影。增强动脉期表现为结节中心低密度无强化区、周边为高密度强化环,即呈“牛眼征”2例;结节均匀强化2例;结节中心小结节样强化、周边低密度环、外周环状强化1例。所有活动期病灶周围肝实质动脉期均伴有一过性高密度灌注区。门脉期及延迟期病灶边缘仍可见环形强化,强化范围较动脉期缩小2例、呈花环样强化2例、外周强化环消退1例。脾脏和肾脏病灶表现与肝脏类似;肺部结节表现为实性结节或伴空洞。结论血液病免疫抑制治疗后播散性白色念珠菌感染的CT表现有一定特征性,该特征性有助于早期诊断和疗效监测。 展开更多
关键词 血液病 白色念珠菌 免疫抑制 体层摄影术 X线计算机
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2433例真菌性阴道炎患者病原菌分析及耐药性研究 预览
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作者 田际云 陈轼 +1 位作者 杨永刚 姚敏婕 《医药前沿》 2019年第14期14-16,共3页
目的:了解杭州地区真菌性阴道炎患者阴道分泌物病原菌的分布及其耐药情况,为临床诊疗提供依据.方法:选取2017年5月-2018年6月浙江省杭州市第三人民医院收治的2433例真菌性阴道炎患者,对其阴道分泌物进行真菌培养、鉴定和药敏试验,并对... 目的:了解杭州地区真菌性阴道炎患者阴道分泌物病原菌的分布及其耐药情况,为临床诊疗提供依据.方法:选取2017年5月-2018年6月浙江省杭州市第三人民医院收治的2433例真菌性阴道炎患者,对其阴道分泌物进行真菌培养、鉴定和药敏试验,并对结果进行统计学分析.结果:A组为20岁以下的病人共165例,占6.8%;B组为21~30岁,共659例,占27.1%;C组为31~40岁,共743例,占30.5%;D组为41~50岁,共637例,占26.2%;E组为大于50岁的患者,共229例,占9.4%.引起妇女真菌性阴道炎的致病菌主要是白念珠菌、光滑念珠菌、热带念珠菌和近平滑念珠菌,所占比例分别为76.0%、12.5%、7.9%和1.9%.各种念珠菌对两性霉素B均为100%敏感;除0.5%的白念珠菌对5-氟胞嘧啶中介外,其他念珠菌均对5-氟胞嘧啶均为100%敏感;白念珠菌、光滑念珠菌、热带念珠菌、近平滑念珠菌对氟康唑敏感率依次为:95.3%、98%、95.3%和95.7%,对伏立康唑的敏感率依次为98.9%、94.8%、96.9%和93.6%,对伊曲康唑敏感率的波动较大,依次为89.4%,55.7%、50.3%和91.5%.结论:育龄女性为真菌性阴道炎好发群体,病原菌以白念珠菌为主,念珠菌对常见的抗真菌药物较为敏感,但耐药菌株也在逐渐增多,需引起临床重视. 展开更多
关键词 念珠菌 真菌性阴道炎 药敏试验 耐药分析 抗真菌药物
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