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Toxoplasma ROP16I/III ameliorated inflammatory bowel diseases via inducing M2 phenotype of macrophages 预览
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作者 Yong-Wei Xu Rui-Xin Xing +7 位作者 Wen-Hui Zhang Lu Li Yi Wu Jing Hu Cong Wang Qing-Li Luo Ji-Long Shen Xi Chen 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第45期6634-6652,共19页
BACKGROUND Inflammatory bowel disease(IBD)is characterized by chronic and non-specific inflammation of the intestinal mucosa and mainly includes ulcerative colitis and Crohn's disease.AIM To explore the beneficial... BACKGROUND Inflammatory bowel disease(IBD)is characterized by chronic and non-specific inflammation of the intestinal mucosa and mainly includes ulcerative colitis and Crohn's disease.AIM To explore the beneficial effect of ToxoROP16I/III-induced M2 phynotype macrophages in homeostasis of IBDs through downregulation of M1 inflammatory cells.METHODS RAW264.7 macrophages stimulated by lipopolysaccharide(LPS)(M1 cells)were co-cultured with Caco-2 cells as an inflammatory model of IBD in vitro.The expression of ToxoROP16I/III was observed in RAW264.7 macrophages that were transfected with pEGFP-rop16I/III.The phenotypes of M2 and M1 macrophage cells were assessed by quantitative real-time reverse transcriptase polymerase chain reaction and the expression of tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6,transforming growth factor(TGF)-β1,IL-10,inducible nitric oxide synthase(iNOS),and arginase-1(Arg-1)was detected.The expression of iNOS,Arg-1,signal transducer and activator of transcription 3(Stat3),p-Stat3,Stat6,p-Stat6,programmed death ligand-2(PD-L2),caspase-3,-8,and-9 was analyzed by Western blotting,and Griess assays were performed to detect nitric oxide(NO).TNF-α,IL-1β,IL-6,TGF-β1,and IL-10 expression in the supernatants was detected by enzyme-linked immunosorbent assay,and Caco-2 cell apoptosis was co-culture system.RESULTS M1 cells exhibited significantly increased production of iNOS,NO,TNF-α,IL-1β,and IL-6,while ToxoROP16I/III induced macrophage bias to M2 cells in vitro,showing increased expression of Arg-1,IL-10 and TGF-β1 and elevated production of p-Stat3 and p-Stat6.The mixed M1 and M2 cell culture induced by ToxoROP16I/III exhibited decreased production of NO and iNOS and upregulated expression of Arg-1 and PD-L2.Accordingly,Caco-2 cells became apoptotic,and apoptosis-associated proteins such as caspase-3,-8 and-9 were dampened during co-culture of M1 and M2 cells.Flow cytometry analysis showed that co-culture of M1 cells with Caco-2 cells facilitated the apoptosis of Caco-2 cells,but co 展开更多
关键词 Toxoplasma ROP16I/III CACO-2 Inflammatory bowel disease IMMUNITY Classically activated macrophages Alternatively activated macrophages
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Influence of contact pressure and sliding speed dependence on the tribological characteristics of an activated carbonepoxy composite derived from palm kernel under dry sliding conditions
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作者 Dayang Nor Fatin MAHMUD Mohd Fadzli Bin ABDOLLAH +2 位作者 Nor Azmmi Bin MASRIPAN NoreffendyTAMALDIN Hilmi AMIRUDDIN 《摩擦(英文版)》 CSCD 2019年第3期227-236,共10页
The objective of this work is to investigate the influence of contact pressure and sliding speed on the coefficient of friction and wear of an activated carbon-epoxy composite derived from palm kernel under dry slidin... The objective of this work is to investigate the influence of contact pressure and sliding speed on the coefficient of friction and wear of an activated carbon-epoxy composite derived from palm kernel under dry sliding conditions.A wear mode map approach was employed to identify the transitions from mild to severe wear of the composite.The dry sliding test was executed by utilizing a ball-on-disc tribometer at different contact pressures and sliding speeds with a constant sliding distance and operating temperature.The results showed that,regardless of the sliding speed,the friction coefficient and wear rate of the composite increased drastically when a critical limit of contact pressure is exceeded.As for the sliding speed,both the friction coefficient and wear rate increased first and thereafter decreased at a higher speed of 500 rpm.A wear mode map is proposed to classify the boundary from mild to severe wear regimes.The predominant wear failures identified include micro-crack,fine grooves,debonding,delamination,debris,broken carbon,and fracture. 展开更多
关键词 activated carbon agriculture waste PALM KERNEL FRICTION and WEAR pressure and speed
Extract of Fructus Schisandrae chinensis Inhibits Neuroinflammation Mediator Production from Microglia via NF-κB and MAPK Pathways
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作者 SONG Fang-jiao ZENG Ke-wu +4 位作者 CHEN Jin-feng LI Yuan SONG Xiao-min TU Peng-fei WANG Xue-mei 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第2期131-138,共8页
Objective: To investigate the anti-neuroinflammation effect of extract of Fructus Schisandrae chinensis(EFSC) on lipopolysaccharide(LPS)-induced BV-2 cells and the possible involved mechanisms. Methods: Primary cortic... Objective: To investigate the anti-neuroinflammation effect of extract of Fructus Schisandrae chinensis(EFSC) on lipopolysaccharide(LPS)-induced BV-2 cells and the possible involved mechanisms. Methods: Primary cortical neurons were isolated from embryonic(E17-18) cortices of Institute of Cancer Research(ICR) mouse fetuses. Primary microglia and astroglia were isolated from the frontal cortices of newborn ICR mouse. Different cells were cultured in specific culture medium. Cells were divided into 5 groups: control group, LPS group(treated with 1 μg/mL LPS only) and EFSC groups(treated with 1 μg/mL LPS and 100, 200 or 400 mg/mL EFSC, respectively). The effect of EFSC on cells viability was tested by methylthiazolyldiphenyltetrazolium bromide(MTT) colorimetric assay. EFSC-mediated inhibition of LPS-induced production of pro-inflammatory mediators, such as nitrite oxide(NO) and interleukin-6(IL-6) were quantified and neuron-protection effect against microglia-mediated inflammation injury was tested by hoechst 33258 apoptosis assay and crystal violet staining assay. The expression of pro-inflammatory marker proteins was evaluated by Western blot analysis or immunofluorescence. Results: EFSC(200 and 400 mg/mL) reduced NO, IL-6, inducible nitric oxide synthase(iN OS) and cyclooxygenase 2(COX-2) expression in LPS-induced BV-2 cel s(P<0.01 or P<0.05). EFSC(200 and 400 mg/mL) reduced the expression of NO in LPS-induced primary microglia and astroglia(P<0.01). In addition, EFSC al eviated cel apoptosis and inflammation injury in neurons exposed to microglia-conditioned medium(P<0.01). The mechanistic studies indicated EFSC could suppress nuclear factor(NF)-κB phosphorylation and its nuclear translocation(P<0.01). The anti-inflammatory effect of EFSC occurred through suppressed activation of mitogen-activated protein kinase(MAPK) pathway(P<0.01 or P<0.05). Conclusion: EFSC acted as an anti-inflammatory agent in LPS-induced glia cel s. These effects might be realized through blocking of NF-κB activity and inhibition of M 展开更多
关键词 NEUROINFLAMMATION MICROGLIA Fructus Schisandrae CHINENSIS nuclear factor-κB mitogen- activated protein KINASE
Calcineurin/Nuclear Factor of Activated T-Cell Pathway in Cutaneous Squamous Cell Carcinoma
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作者 Yi Wu Feng-Juan Li +4 位作者 Ke Zhang Qun Lv Xue-Yuan Yang Li-Ming Li Ming-Jun Jiang 《国际皮肤性病学杂志(英文)》 2019年第3期156-159,共4页
Introduction Cutaneous squamous cell carcinoma (CSCC),a keratinocyte-derived skin neoplasm with malignant potential,1 represents 20%-50% of skin cancers and currently has an increasing incidence in the United States.2... Introduction Cutaneous squamous cell carcinoma (CSCC),a keratinocyte-derived skin neoplasm with malignant potential,1 represents 20%-50% of skin cancers and currently has an increasing incidence in the United States.2 Ultraviolet (UV)solar radiation is the primary risk factor for the development of CSCC,and the cumulative exposure received over a lifetime plays a major role in this development.3 Mutations in the p53 gene are the most common genetic abnormalities,causing nonfunctional p53 protein production and cells with damaged DNA replicate in CSCC. 展开更多
关键词 Calcineurin/Nuclear Factor ACTIVATED T-CELL Cell CARCINOMA
Effects of temperature and SO3 on re-emission of mercury from activated carbon under flue gas conditions
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作者 Michael Royko Benjamin Galloway +1 位作者 Noah D.Meeks Bihter Padak 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第5期67-73,共7页
Mercury(Hg) is a toxic and bio-accumulating heavy metal that is predominantly released via the combustion of coal. Due to its toxicity, the Environmental Protection Agency(EPA)has introduced Mercury and Air Toxics Sta... Mercury(Hg) is a toxic and bio-accumulating heavy metal that is predominantly released via the combustion of coal. Due to its toxicity, the Environmental Protection Agency(EPA)has introduced Mercury and Air Toxics Standards(MATS) Rule regarding allowable Hg emissions. In order to reduce emissions, power plants have widely adopted activated carbon(AC) injection. AC injection has proven to be an effective method to reduce Hg emissions, but the re-emission of previously adsorbed Hg during unit operation, likely due to changing temperature or flue gas composition, could be problematic. This study specifically examined the effects of temperature and sulfur trioxide(SO3) concentration,by ramping temperature and SO3 concentration independently and simultaneously, on AC samples that are already exposed to flue gas and saturated in presence of Hg, sulfur dioxide(SO2) and nitric oxide(NO). Of these two suspected factors to cause re-emission,temperature had the greater impact and resulted in re-emission of both elemental and oxidized Hg with a greater fraction of oxidized Hg, which can be attributed to elemental Hg being more strongly bonded to the AC surface. Surprisingly, exposing the samples to increasing concentrations of SO3 had nearly no effect under the conditions examined in this study, possibly as a result of the samples being already saturated with sulfur prior to the SO3 ramp tests to simulate transient conditions in the plant. 展开更多
关键词 MERCURY re-emission Sulfur TRIOXIDE Activated carbon
Ozone-Biological Activated Carbon. Advanced Removal. Typical Persistent Organic pollutants from Micropolluted Source Water. Yangtze DeltaREgion 预览
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作者 Yaqiong Li 《探索-环境科学与技术》 2019年第1期18-21,共4页
Ozone-Biological Activated Carbon(O3-Bac). Studied. Advanced Removal. organics from micropolluted Source Water. water supply plant P located. J City. yangtze DeltaREgion..: Show, 19. 2%. permanganate Index10. 4%. tota... Ozone-Biological Activated Carbon(O3-Bac). Studied. Advanced Removal. organics from micropolluted Source Water. water supply plant P located. J City. yangtze DeltaREgion..: Show, 19. 2%. permanganate Index10. 4%. total organic carbon23. 0% UV254. Removed. advanced treatment. O3-Bac.Eight types. polycyclic aromatic Hydrocarbon(PAH)16 types. organochlorinated pesticides(OCPs), Five types. haloacetic Acids(Haas). Detected. Source Water.. Total concentrations. 53. 9-100. 06. 5-41. 8, 2. 5 × 10^3-1. 1 × 10^4Ng in L^-1... Advanced O3-Bac treatment removed 32. 5%. PAHs 25. 9%. OCPsGreatly improving. effluent. conventional Water Supply Process.HoweverHaas. mainly removed. conventional process. A removal. 33. 8%-0%.After. advanced treatment. O3-Bac. Chloroacetic acid slightly decreasedWhile the concentration of bromokinetic acid slightly created. 展开更多
关键词 Ozone-Biological Activated Carbon(O3.-Bac) POLYCYCLIC aromatic hydrocarbons(PAHs) ORGANIC Pesticides(OCPs) HALAL kids(Haas) Yangtza DeltaREgion Persistent ORGANIC pollutants(POPs)
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Removal of CO2 in a multistage fluidized bed reactor by amine impregnated activated carbon: optimization using response surface methodology 预览
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作者 Dipa Das Sushanta K. Behera B. C. Meikap 《国际煤炭科学技术学报:英文版》 2019年第3期445-458,共14页
Carbon dioxide (CO2) is the major component of greenhouse gas. Increase in concentration of CO2 in the atmosphere leads to global warming. To remove the CO2 from waste flue gas a four-stage counter-current multistage ... Carbon dioxide (CO2) is the major component of greenhouse gas. Increase in concentration of CO2 in the atmosphere leads to global warming. To remove the CO2 from waste flue gas a four-stage counter-current multistage fluidized bed adsorber was developed and operated in continuous bubbling fluidization regime for the two丒phase system. This paper describes the optimum condition for CO2 removal efficiency in a multistage fluidized bed reactor using amine impregnated activated carbon. Response surface methodology with central composite design was used to determine the effect of three variables on the response. The variables are inlet concentration of CO2 in ppm (ranging from 3000 to 20,000), impregnation ratio of monoethanol amine (ranging from 0.2 to 0.6) and weir height in mm (20-60). The response was CO2 removal efficiency. The factor which was most influential has been identified from the analysis of variance. The optimum CO2 removal efficiency for the amine impregnated activated carbon (MEA-AC) was found to be 95.17%, at initial concentration of CO2 7312.85 ppm, chemical impregnation ratio of 0.31, and weir height 48.65 mm. From the experiment, the CO2 removal efficiency was found to be 95.97% at the same operating conditions. The predicted response was found to relevance with experimental data. 展开更多
关键词 Activated carbon Response surface methodology COCONUT shell IMPREGNATION ratio MULTISTAGE fluidized BED OPTIMIZATION
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Unexpected activated carbon-catalyzed pyrrolo[1,2-a]quinoxalines synthesis in water
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作者 Qi Sun Liyan Liu +2 位作者 Yu Yang Zhenggen Zha Zhiyong Wang 《中国化学快报:英文版》 SCIE CAS CSCD 2019年第7期1379-1382,共4页
An interesting and recyclable activated carbon/water catalytic system for efficient synthesis of pyrrolo[1,2-a]quinoxaline derivatives was developed. The intramolecular C-N and C-C bond can be easily constructed in wa... An interesting and recyclable activated carbon/water catalytic system for efficient synthesis of pyrrolo[1,2-a]quinoxaline derivatives was developed. The intramolecular C-N and C-C bond can be easily constructed in water under mild condition. This reaction features a broad substrate scope, a good tolerance to water and air, metal-free, additive-free and redox reagent-free. 展开更多
关键词 Pyrrolo[1 2-a]quinoxalines Activated carbon C-C C-N bond-forming Metal free WATER SOLVENT
Experimental evaluation of activated carbon derived from South Africa discard coal for natural gas storage 预览
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作者 Jibril Abdulsalam Jean Mulopo +2 位作者 Bilainu Oboirien Samson Bada Rosemary Falcon 《国际煤炭科学技术学报:英文版》 2019年第3期459-477,共19页
Lacking in literature is the use of discard coal to produce activated carbon and in its subsequent use in the storage of natural gas. In this study, the characterization and gas storage evaluation of a largely porous ... Lacking in literature is the use of discard coal to produce activated carbon and in its subsequent use in the storage of natural gas. In this study, the characterization and gas storage evaluation of a largely porous activated carbon with large surface area synthesized from discard coal were investigated. Discard coals are waste material generated from coal beneficiation process. In developing the activated carbon, chemical activation route with the use of KOH reagent was applied. The effects of KOH/discard coal weight ratio (1:1, 2.5:1, 4:1), temperature (400-800 ℃) and particle size (0.15-0.25 mm, 0.25-0.5 mm, 0.5-1 mm) on the adsorptive properties of the activated carbon were methodically evaluated and optimized using response surface methodology. The synthesized activated carbon was characterized using BET, SEM/EDS, and XRD. The results showed that for each activation process, the surface area and pore volume of the resulting activated carbon increased with increased temperature and KOH/discard coal weight ratio. The maximum surface area of 1826.41 m2/g, pore volume of 1.252 cm^3/g and pore size of 2.77 nm were obtained at carbonization temperature of 800 ℃ and KOH/discard coal weight ratio of 4:1. Methane and nitrogen adsorption data at high pressure were fitted to Toth isotherm model with a predictive accuracy of about 99%. Adsorption parameters using the Toth model provides useful information in the design of adsorbed natural gas storage system. According to the requirements of adsorbent desired for natural gas storage, it could be stated that the synthesized activated carbon could well be applied for natural gas storage. 展开更多
关键词 DISCARD COAL ACTIVATED carbon Surface area METHANE ADSORPTION
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Preparation and Adsorption Properties of Biomass Activated Carbon from Ginger Stems 预览
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作者 Jinling Liu Xiaoming Song +1 位作者 Shanshan Gao Fushan Chen 《造纸与生物质材料:英文版》 2019年第4期45-50,共6页
Biomass activated carbon(BAC)was produced from ginger stems by carbonization and activation presented high specific surface areas and mesoporous structures.The carbonization temperature of the ginger stems were contro... Biomass activated carbon(BAC)was produced from ginger stems by carbonization and activation presented high specific surface areas and mesoporous structures.The carbonization temperature of the ginger stems were controlled within 500~900℃.The optimal carbonization condition is as follows:carbonization temperature of 700℃,carbonization time of 6 h.The determined optimum activation condition is:temperature of 800℃,activator of KOH and carbonized product/alkali ratio of 1:4(w/w).The carbonization yield,BAC yield and Brunauer-Emmett-Teller(BET)surface area were measured and the adsorption performance of BAC to nitrogen was investigated.The results showed that the nitrogen adsorption isotherm curve was as type I isotherm.It was finally determined that the BET surface area was 660 m2/g under the abovementioned optimal conditions of carbonization and activation.The FESEM analysis indicates that the obtained BAC is of micropore structure. 展开更多
关键词 ginger stems biomass activated carbon CARBONIZATION activation
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Microstructure and magnetic properties of Nd-Fe-B sintered magnets with P addition
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作者 Fang Yang Guang-Le Dong +5 位作者 Yan-Li Sui Si-Yang Ye Ping Li Cun-Guang Chen Xue-Xu Gao Zhi-Meng Guo 《稀有金属:英文版》 SCIE EI CAS CSCD 2019年第8期770-775,共6页
In this paper,the effects of P doping on magnetic properties and microstructure were studied in Nd-Fe-B sintered magnets.With P doping,the grain size gets refined and the distribution of the main phase is optimized du... In this paper,the effects of P doping on magnetic properties and microstructure were studied in Nd-Fe-B sintered magnets.With P doping,the grain size gets refined and the distribution of the main phase is optimized due to the reduction of the liquidus temperature.The liquidus temperature for the 0.05 wt% P-doped magnets is 1022 K,while that for the P-free magnets is 1038 K.As P content increases,the liquidus temperature significantly decreases.Clear and continuous grain boundary phases are formed in the P-containing magnets with smaller grain size.The optimized microstructure with average grain size of 8.43 μm is obtained in the 0.05 wt% P-doped magnets,which is approximately 0.69 μm smaller than that of P-free sintered magnets (9.12 μm).Though P is usually thought to be an impurity element,it might be beneficial in Nd-Fe-B sintered magnets with proper addition.The coercivity of the 0.05 wt% P-doped magnets could be increased to 1283 kA·m-1,with slight changes of the remanence and the maximum magnetic energy product.NdPO4 phases in the grain boundary are of hexagonal structure,while those at the triple junctions have monoclinic structure.Activated sintering is achieved by doping proper P element in the Nd-Fe-B sintered magnets. 展开更多
关键词 ND-FE-B sintered MAGNETS P MICROSTRUCTURE MAGNETIC properties Activated SINTERING
Continuous flow removal of acid fuchsine by dielectric barrier discharge plasma water bed enhanced by activated carbon adsorption
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作者 Rusen Zhou Renwu Zhou +2 位作者 Xianhui Zhang Kateryna Bazaka Kostya (Ken) Ostrikov 《化学科学与工程前沿:英文版》 EI CAS CSCD 2019年第2期340-349,共10页
Continuous processes which allow for large amount of wastewater to be treated to meet drainage standards while reducing treatment time and energy consumption are urgently needed. In this study, a dielectric barrier di... Continuous processes which allow for large amount of wastewater to be treated to meet drainage standards while reducing treatment time and energy consumption are urgently needed. In this study, a dielectric barrier discharge plasma water bed system was designed and then coupled with granular activated carbon (GAC) adsorption to rapidly remove acid fuchsine (AF) with high efficiency. Effects of feeding gases, treatment time and initial concentration of AF on removal efficiency were investigated. Results showed that compared to the N2 and air plasmas treatments, O2 plasma processing was most effective for AF degradation due to the strong oxidation ability of generated activated species, especially the OH radicals. The addition of GAC significantly enhanced the removal efficiency of AF in aqueous solution and shorten the required time by 50%. The effect was attributed to the ability of porous carbon to trap and concentrate the dye, increasing the time dye molecules were exposed to the plasma discharge zone, and to enhance the production of OH radicals on/in GAC to boost the degradation of dyes by plasma as well as in situ regenerate the exhausted GAC. The study offers a new opportunity for continuous effective remediation of wastewater contaminated with organic dyes using plasma technologies. 展开更多
关键词 continuous REMOVAL dye-containing WASTEWATER dielectric barrier discharge plasma WATER BED activated carbon adsorption
Application of activated carbon-decorated polyacrylonitrile nanofibers as an adsorbent in dispersive solid-phase extraction of fluoroquinolones from wastewater 预览
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作者 K. Mogolodi Dimpe Philiswa N. Nomngongo 《药物分析学报:英文版》 CAS CSCD 2019年第2期117-126,共10页
A cheap and simple sample preparation method,consisting of a dispersive solid-phase method and an adsorbent,activated carbon decorated PAN nanofibers,was employed and used for the extraction of antibiotics (ciprofloxa... A cheap and simple sample preparation method,consisting of a dispersive solid-phase method and an adsorbent,activated carbon decorated PAN nanofibers,was employed and used for the extraction of antibiotics (ciprofloxacin,danofloxacin,and enrofloxacin) in wastewater.Electrospun PAN nanofibers that were decorated with activated carbon produced from waste tires were used as the solid phase and the antibiotics analyzed by using high-performance liquid chromatography.Parameters such as pH,mass of adsorbent (MA),extraction volume (EV),and extraction time (ET) were optimized owing to their potential effect on the extraction of antibiotics from water.The recovery of all antibiotics was satisfactory,in the range of 90%–99%.The limits of detection and quantification were 0.05,0.11,0.20,and 0.53,1.21,2.17 mg/L,respectively.The precision was determined from the repeatability and reproducibility and expressed as the intra-day (n=20) and inter-day (n=5) precision.The intra-day and inter-day precision was reported in terms of the percentage relative standard deviation,which was 3% and 4%,respectively.The adsorption capacity of the activated carbon-decorated PAN nanofibers was satisfactory,and the reusability of the adsorbent was impressive when reused ten times.The accuracy of the dispersive solid phase extraction (DSPE) was validated by spike recovery tests;the results proved the reliability and efficiency of adsorbing antibiotics from wastewater.Finally,the proposed method was applied to wastewater samples collected from a wastewater treatment plant,which included influent,secondary,and effluent wastewater. 展开更多
关键词 WASTEWATER NANOFIBERS Activated carbon ANTIBIOTICS POLYACRYLONITRILE
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Upgrading VFAs bioproduction from waste activated sludge via co-fermentation with soy sauce residue
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作者 Yanqing Duan Aijuan Zhou +4 位作者 Kaili Wen Zhihong Liu Wenzong Liu Aijie Wang Xiuping Yue 《中国环境科学与工程前沿:英文版》 SCIE EI CAS CSCD 2019年第1期53-62,共10页
Conditioning of extra carbon sources has been widely reported to facilitate fermentation of waste activated sludge (WAS). Soy sauce residue (SSR) was a relatively untapped carbon source for sludge conditioning. This b... Conditioning of extra carbon sources has been widely reported to facilitate fermentation of waste activated sludge (WAS). Soy sauce residue (SSR) was a relatively untapped carbon source for sludge conditioning. This batch study aimed to evaluate the possible implementation of SSR for volatile fatty acids (VFAs) production from WAS. To upgrade the bioavailability of feedstock, three typical pretreatment methods were conducted, i.e., ammonium hydroxide (AH), sulfuric acids (SA) and thermal assisted alkaline (TA). AH pretreated test (AH-PT) outperformed due to a relatively strong structure decomposition of cellulosic materials as revealed by infrared spectroscopic analysis and crystal index. As a result, performed a high hydrolysis rate of 4449 mg COD/d, 1.12-1.23-fold higher than that in TA and SA pretreated tests (TA-PT and SA-PT), and 7.8-fold higher than that in the Control test. Meanwhile, a volatile fatty acids (VFAs) contribution of 401.2 mg COD/g SSR L and a maximum acidification rate of 3.59 d was recorded, with a high sum proportion of mall molecular acetic and propionic 82.2%, 11%-70% increase over the other three tests. Besides, speciation process characterized with functional genus differentiation was identified by microbial diversity and distribution investigation and canonical correspondence analysis (CCA). Finally, a potential market value of 0.49-0.65 Billion €/year was preliminary estimated, showing promise of resource recovery from both WAS and SSR instead of extensive disposal. 展开更多
关键词 Waste activated SLUDGE (WAS) SOY SAUCE residue (SSR) SLUDGE conditioning VOLATILE fatty acids (VFAs) Microbial diversity
Acute and chronic toxicity of nickel to nitrifiers at different temperatures
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作者 Xiaoguang Liu Mohammad M.I. Chowdhury +2 位作者 Masuduz Zaman Mingu Kim George Nakhla 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第8期169-178,共10页
This study investigated the acute nickel toxicity on nitrification of low ammonia synthetic wastewater at 10, 23, and 35°C. The nickel inhibition half-velocity constants(KI,Ni) for ammonia oxidizing bacteria(AOB)... This study investigated the acute nickel toxicity on nitrification of low ammonia synthetic wastewater at 10, 23, and 35°C. The nickel inhibition half-velocity constants(KI,Ni) for ammonia oxidizing bacteria(AOB) and nitrite oxidizing bacteria(NOB) based on Ni/MLSS ratio at 10, 23, and 35°C were 5.4 and 5.6 mg Ni/g MLSS, 4.6 and 3.5 mg Ni/g MLSS, and 9.1 and 2.7 mg Ni/g MLSS, respectively. In addition, chronic toxicity of nickel to nitrification of low ammonia synthetic wastewater was investigated at 10°C in two sequencing batch reactors(SBRs). Long-term SBRs operation and short-term batch tests were comparable with respect to the extent of inhibition and corresponding Ni/MLSS ratio. The μmax, b, and Ko of AOB were 0.16 day^-1, 0.098 day^-1 and 2.08 mg O2/L after long-term acclimatization to nickel of 1 mg/L at 10°C, high dissolved oxygen(DO)(7 mg/L) and long solids retention time(SRT) of 63–70 days. Acute nickel toxicity of nitrifying bacteria was completely reversible. 展开更多
关键词 NICKEL INHIBITION Temperature Nitrifying culture ACTIVATED SLUDGE TOXICITY
Roles of extra-cellular signal-regulated protein kinase 5 signaling pathway in the development of spinal cord injury
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作者 Chen-Jun Liu Hai-Ying Liu +3 位作者 Zhen-Qi Zhu Yuan-Yuan Zhang Kai-Feng Wang Wei-Wei Xia 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第21期2601-2611,共11页
Background:In consideration of characteristics and functions,extra-cellular signal-regulated protein kinase 5(ERK5)signaling pathway could be a new target for spinal cord injury(SCI)treatment.Our study aimed to evalua... Background:In consideration of characteristics and functions,extra-cellular signal-regulated protein kinase 5(ERK5)signaling pathway could be a new target for spinal cord injury(SCI)treatment.Our study aimed to evaluate the roles of ERK5 signaling pathway in secondary damage of SCI.Methods:We randomly divided 70 healthy Wistar rats into five groups:ten in the blank group,15 in the sham surgery+BIX02188(sham+B)group,15 in the sham surgery+dimethyl sulfoxide(DMSO;sham+D)group,15 in the SCI+BIX02188(SCI+B)group,and 15 in the SCI+DMSO(SCI+D)group.BIX02188 is a specific inhibitor of the ERK5 signaling pathway.SCI was induced by the application of vascular clips(with the force of 30 g)to the dura on T10 level,while rats in the sham surgery group underwent only T9-T11 laminectomy.BIX02188 or DMSO was intra-thecally injected at 1,6,and 12 h after surgery or SCI.Spinal cord samples were taken for testing at 24 h after surgery or SCI.Results:Expression of phosphorylated-ERK5(p-ERK5)significantly increased after SCI.Application of BIX02188 indeed inhibited ERK5 signaling pathway and reduced the degree of spinal cord tissue injury,neutrophil infiltration and proinflammatory cytokine expression,nuclear factor-kB(NF-kB)activation and apoptosis(measured by TdT-mediated 20-deoxyuridine 50-triphosphate nickend labeling,expression of Fas-ligand,BCL2-associated X[Bax],and B-cell lymphoma-2[Bcl-2]).Double immunofluorescence revealed activation of ERK5 in neurons and microglia after SCI.Conclusion:ERK5 signaling pathway was activated in spinal neurons and microglia,contributing to secondary injury of SCI.Moreover,inhibition of ERK5 signaling pathway could alleviate the degree of SCI,which might be related to its regulation of infiltration of inflammatory cells and release of inflammatory cytokines,expression of NF-kB and cell apoptosis. 展开更多
关键词 Extracellular signal-regulated protein kinase 5 Mitogen activated protein kinase Spinal cord injury Nuclear factor-kB Apoptosis
A strategy for enhancing anaerobic digestion of waste activated sludge: Driving anodic oxidation by adding nitrate into microbial electrolysis cell
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作者 Hong Peng Zhiqiang Zhao +3 位作者 Hong Xiao Yafei Yang Huimin Zhao Yaobin Zhang 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第7期34-42,共9页
Cathodic reduction of CO2 and anodic oxidation of organic matters are crucial to methaneproducing microbial electrolysis cell(MEC) applied in anaerobic digestion of waste activated sludge. However, cathodic CO2 reduct... Cathodic reduction of CO2 and anodic oxidation of organic matters are crucial to methaneproducing microbial electrolysis cell(MEC) applied in anaerobic digestion of waste activated sludge. However, cathodic CO2 reduction is usually restrained by slow metabolism rates of H2-utilizing methanogens and low electron-capturing capacity of CO2, which consequently slows down the anodic oxidation that participates to sludge disintegration. Herein, a strategy with adding nitrate as electron acceptor to foster electronic transfer between the anode and cathode was proposed to improve anodic oxidation. Results showed that the average efficiency of anodic oxidation in the nitrate-added MEC increased by 55.9%. Accordingly,volatile suspended solid removal efficiency in the nitrate-added MEC was 21.9% higher than that of control MEC. Although the initial cumulative methane production in the nitrateadded MEC was lower than that of control MEC, the cumulative methane production in 24 days was 8.9% higher. Fourier transform infrared spectroscopy analysis indicated that anodic oxidation of MEC with nitrate accelerated the disintegration of sludge flocs and cell walls. Calculation on current signal further revealed that anodic oxidation driven by cathodic nitrate reduction was the main mechanism responsible for the improved sludge digestion. 展开更多
关键词 Waste ACTIVATED SLUDGE MICROBIAL ELECTROLYSIS cell NITRATE Anodic oxidation SLUDGE reduction
Strengthening decomposition of oxytetracycline in DBD plasma coupling with Fe-Mn oxide-loaded granular activated carbon
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作者 唐首锋 李雪 +3 位作者 张晨 刘洋 张维涛 袁德玲 《等离子体科学与技术:英文版》 SCIE EI CAS CSCD 2019年第2期90-96,共7页
A catalytic approach using a synthesized iron and manganese oxide-supported granular activated carbon(Fe-Mn GAC) under a dielectric barrier discharge(DBD) plasma was investigated to enhance the degradation of oxytetra... A catalytic approach using a synthesized iron and manganese oxide-supported granular activated carbon(Fe-Mn GAC) under a dielectric barrier discharge(DBD) plasma was investigated to enhance the degradation of oxytetracycline(OTC) in water. The prepared Fe-Mn GAC was characterized by x-ray diffraction and scanning electron microscopy, and the results showed that the bimetallic oxides had been successfully spread on the GAC surface. The experimental results showed that the DBD?+?Fe-Mn GAC exhibited better OTC removal efficiency than the sole DBD and DBD?+?virgin GAC systems. Increasing the fabricated catalyst and discharge voltage was favorable to the antibiotic elimination and energy yield in the hybrid process. The coupling process could be elucidated by the ozone decomposition after Fe-Mn GAC addition, and highly hydroxyl and superoxide radicals both play significant roles in the decontamination. The main intermediate products were identified by HPLC-MS to study the mechanism in the collaborative system. 展开更多
关键词 dielectric barrier discharge plasma Iron and MANGANESE OXIDES OXYTETRACYCLINE DECOMPOSITION supported GRANULAR activated carbon
Polymorphism dependent triplet-involved emissions of a pure organic luminogen
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作者 Zihan He Wenbo Li +2 位作者 Gan Chen Yongming Zhang Wang-Zhang Yuan 《中国化学快报:英文版》 SCIE CAS CSCD 2019年第4期933-936,共4页
Polymorphism has been frequently used in tuning the singlet emissions of pure organic dyes. The modulation of triplet-involved emissions, particularly room temperature phosphorescence (RTP), however, is scarcely repor... Polymorphism has been frequently used in tuning the singlet emissions of pure organic dyes. The modulation of triplet-involved emissions, particularly room temperature phosphorescence (RTP), however, is scarcely reported. Herein, polymorphism is reported to tune the triplet-involved emissions of 2CZBZL, a newly designed pure organic luminogen consisting of twisted benzil and two planar carbazole moieties. Other than the conventional modulation through changing molecular conformation and packing, vibration can also finely tune the triplet-involved emissions. Besides prompt fluorescence (PF), polymorph B with relatively extended conformation emits thermally activated delayed fluorescence (TADF), whereas the others (A, C-E) with similarly more twisted conformations generate predominant RTP or simultaneous DF and RTP. These results demonstrate the fascinating chance to regulate the tripletinvolved emissions through controlling conformation and vibration. 展开更多
关键词 POLYMORPHISM Room temperature phosphorescence PURE ORGANIC luminogens Thermally activated delayed fluorescence Triplet-involved EMISSIONS
Long-and short-chain AHLs affect AOA and AOB microbial community composition and ammonia oxidation rate in activated sludge
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作者 Jie Gao Yu Duan +5 位作者 Ying Liu Xuliang Zhuang Yichen Liu Zhihui Bai Wenlin Ma Guoqiang Zhuang 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第4期53-62,共10页
Quorum sensing(QS)regulation of the composition of ammonia-oxidising archaea(AOA)and ammonia-oxidising bacteria(AOB)communities and functions in wastewater treatment was investigated.Specifically,we explored the role ... Quorum sensing(QS)regulation of the composition of ammonia-oxidising archaea(AOA)and ammonia-oxidising bacteria(AOB)communities and functions in wastewater treatment was investigated.Specifically,we explored the role of N-acyl-L-homoserine lactones(AHLs)in microbial community dynamics in activated sludge.On average,the specific ammoniaoxidising-rate increased from 1.6 to 2.8 mg NH4+-N/g MLSS/hr after treatment with long-chain AHLs for 16 days,and the addition of AHLs to sludge resulted in an increased number of AOA/AOB amoA genes.Significant differences were observed in the AOA communities of control and AHL-treated cultures,but not the AOB community.Furthermore,the dominant functional AOA strains of the Crenarchaeota altered their ecological niche in response to AHL addition.These results provide evidence that AHLs play an important role in mediating AOA/AOB microbial community parameters and demonstrate the potential for application of QS to the regulation of nitrogen compound metabolism in wastewater treatment. 展开更多
关键词 QUORUM sensing Activated sludge Ammonia-oxidising ARCHAEA Ammonia-oxidising bacteria Nitrogen removal Community composition
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