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Molecular complexity of visual mapping:a challenge for regenerating therapy 预览
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作者 Mara Medori Gonzalo Spelzini Gabriel Scicolone 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期382-389,共8页
Investigating the cellular and molecular mechanisms involved in the development of topographically ordered connections in the central nervous system constitutes an important issue in neurobiology because these connect... Investigating the cellular and molecular mechanisms involved in the development of topographically ordered connections in the central nervous system constitutes an important issue in neurobiology because these connections are the base of the central nervous system normal function.The dominant model to study the development of topographic maps is the projection from the retinal ganglion cells to the optic tectum/colliculus.The expression pattern of Eph/ephrin system in opposing gradients both in the retina and the tectum,labels the local addresses on the target and gives specific sensitivities to growth cones according to their topographic origin in the retina.The rigid precision of normal retinotopic mapping has prompted the chemoaffinity hypothesis,positing axonal targeting to be based on fixed biochemical affinities between fibers and targets.However,several lines of evidence have shown that the mapping can adjust to experimentally modified targets with flexibility,demonstrating the robustness of the guidance process.Here we discuss the complex ways the Ephs and ephrins interact allowing to understand how the retinotectal mapping is a precise but also a flexible process. 展开更多
关键词 AXON growth AXON guidance development EPH and EPHRIN mapping regeneration RETINAL GANGLION cells retino-tectal system
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Time course analysis of sensory axon regeneration in vivo by directly tracing regenerating axons 预览
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作者 Yan Gao Yi-Wen Hu +3 位作者 Run-Shan Duan Shu-Guang Yang Feng-Quan Zhou Rui-Ying Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第6期1160-1165,共6页
Most current studies quantify axon regeneration by immunostaining regeneration-associated proteins,representing indirect measurement of axon lengths from both sensory neurons in the dorsal root ganglia and motor neuro... Most current studies quantify axon regeneration by immunostaining regeneration-associated proteins,representing indirect measurement of axon lengths from both sensory neurons in the dorsal root ganglia and motor neurons in the spinal cord.Our recently developed method of in vivo electroporation of plasmid DNA encoding for enhanced green fluorescent protein into adult sensory neurons in the dorsal root ganglia provides a way to directly and specifically measure regenerating sensory axon lengths in whole-mount nerves.A mouse model of sciatic nerve compression was established by squeezing the sciatic nerve with tweezers.Plasmid DNA carrying enhanced green fluorescent protein was transfected by ipsilateral dorsal root ganglion electroporation 2 or 3 days before injury.Fluorescence distribution of dorsal root or sciatic nerve was observed by confocal microscopy.At 12 and 18 hours,and 1,2,3,4,5,and 6 days of injury,lengths of regenerated axons after sciatic nerve compression were measured using green fluorescence images.Apoptosis-related protein caspase-3 expression in dorsal root ganglia was determined by western blot assay.We found that in vivo electroporation did not affect caspase-3 expression in dorsal root ganglia.Dorsal root ganglia and sciatic nerves were successfully removed and subjected to a rapid tissue clearing technique.Neuronal soma in dorsal root ganglia expressing enhanced green fluorescent protein or fluorescent dye-labeled microRNAs were imaged after tissue clearing.The results facilitate direct time course analysis of peripheral nerve axon regeneration.This study was approved by the Institutional Animal Care and Use Committee of Guilin Medical University,China(approval No.GLMC201503010)on March 7,2014. 展开更多
关键词 axon regeneration cell apoptosis dorsal root ganglion in vivo electroporation micro RNAs peripheral nervous system sciatic nerve tissue clearing
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The Schlager mouse as a model of altered retinal phenotype 预览
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作者 Lakshini Y.Herat Aaron L.Magno +5 位作者 Márcio G.Kiuchi Kristy L.Jackson Revathy Carnagarin Geoffrey A.Head Markus P.Schlaich Vance B.Matthews 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期512-518,共7页
Hypertension is a risk factor for a large number of vision-threatening eye disorders.In this study,we investigated for the first time the retinal neural structure of the hypertensive BPH/2J mouse(Schlager mouse)and co... Hypertension is a risk factor for a large number of vision-threatening eye disorders.In this study,we investigated for the first time the retinal neural structure of the hypertensive BPH/2J mouse(Schlager mouse)and compared it to its control counterpart,the normotensive BPN/3J strain.The BPH/2J mouse is a selectively inbred mouse strain that develops chronic hypertension due to elevated sympathetic nervous system activity.When compared to the BPN/3J strain,the hypertensive BPH/2J mice showed a complete loss of outer layers of the neural retina at 21 weeks of age,which was indicative of a severe vision-threatening disease potentially caused by hypertension.To elucidate whether the retinal neural phenotype in the BPH/2J strain was attributed to increased BP,we investigated the neural retina of both BPN/3J and BPH/2J mice at 4 weeks of age.Our preliminary results showed for the first time that the BPH/2J strain develops severe retinal neural damage at a young age.Our findings suggest that the retinal phenotype in the BPH/2J mouse is possibly due to elevated blood pressure and may be contributed by an early onset spontaneous mutation which is yet to be identified or a congenital defect occurring in this strain.Further characterization of the BPH/2J mouse strain is likely to i)elucidate gene defects underlying retinal disease;ii)understand mechanisms leading to neural retinal disease and iii)permit testing of molecules for translational research to interfere with the progression of retinal disease.The animal experiments were performed with the approval of the Royal Perth Hospital Animal Ethics Committee(R535/17-18)on June 1,2017. 展开更多
关键词 blood pressure eye hypertension mice neural regeneration RETINA Schlager MOUSE SYMPATHETIC nervous system
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Characteristics and advantages of adenoassociated virus vector-mediated gene therapy for neurodegenerative diseases 预览
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作者 Yuan Qu Yi Liu +2 位作者 Ahmed Fayyaz Noor Johnathan Tran Rui Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期931-938,共8页
Common neurodegenerative diseases of the central nervous system are characterized by progressive damage to the function of neurons,even leading to the permanent loss of function.Gene therapy via gene replacement or ge... Common neurodegenerative diseases of the central nervous system are characterized by progressive damage to the function of neurons,even leading to the permanent loss of function.Gene therapy via gene replacement or gene correction provides the potential for transformative therapies to delay or possibly stop further progression of the neurodegenerative disease in affected patients.Adeno-associated virus has been the vector of choice in recent clinical trials of therapies for neurodegenerative diseases due to its safety and efficiency in mediating gene transfer to the central nervous system.This review aims to discuss and summarize the progress and clinical applications of adeno-associated virus in neurodegenerative disease in central nervous system.Results from some clinical trials and successful cases of central neurodegenerative diseases deserve further study and exploration. 展开更多
关键词 nerve REGENERATION central nervous system gene therapy NEURODEGENERATIVE DISEASE viral vector ADENO-ASSOCIATED virus Alzheimer’s DISEASE Parkinson’s DISEASE Huntington’s DISEASE amyotrophic lateral SCLEROSIS spinal muscular atrophy neural REGENERATION
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Diffusion kurtosis imaging of microstructural changes in brain tissue affected by acute ischemic stroke in different locations 预览
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作者 Liu-Hong Zhu Zhong-Ping Zhang +2 位作者 Fu-Nan Wang Qi-Hua Cheng Gang Guo 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第2期272-279,共8页
The location of an acute ischemic stroke is associated with its prognosis. The widely used Gaussian model-based parameter, apparent diffusion coefficient (ADC), cannot reveal microstructural changes in different locat... The location of an acute ischemic stroke is associated with its prognosis. The widely used Gaussian model-based parameter, apparent diffusion coefficient (ADC), cannot reveal microstructural changes in different locations or the degree of infarction. This prospective observational study was reviewed and approved by the Institutional Review Board of Xiamen Second Hospital, China (approval No. 2014002). Diffusion kurtosis imaging (DKI) was used to detect 199 lesions in 156 patients with acute ischemic stroke (61 males and 95 females), mean age 63.15 ± 12.34 years. A total of 199 lesions were located in the periventricular white matter (n = 52), corpus callosum (n = 14), cerebellum (n = 29), basal ganglia and thalamus (n = 21), brainstem (n = 21) and gray-white matter junctions (n = 62). Percentage changes of apparent diffusion coefficient (ΔADC) and DKI-derived indices (fractional anisotropy [ΔFA], mean diffusivity [ΔMD], axial diffusivity [ΔDa], radial diffusivity ΔDr, mean kurtosis [ΔMK], axial kurtosis [ΔKa], and radial kurtosis [ΔKr]) of each lesion were computed relative to the normal contralateral region. The results showed that (1) there was no significant difference in ΔADC, ΔMD, ΔDa or ΔDr among almost all locations. (2) There was significant difference in ΔMK among almost all locations (except basal ganglia and thalamus vs. brain stem; basal ganglia and thalamus vs. gray-white matter junctions; and brainstem vs. gray-white matter junctions. (3) The degree of change in diffusional kurtosis in descending order was as follows: corpus callosum > periventricular white matter > brainstem > gray-white matter junctions > basal ganglia and thalamus > cerebellum. In conclusion, DKI could reveal the differences in microstructure changes among various locations affected by acute ischemic stroke, and performed better than diffusivity among all groups. 展开更多
关键词 nerve REGENERATION APPARENT DIFFUSION coefficient DIFFUSION weighted IMAGING DIFFUSION KURTOSIS IMAGING acute ischemic stroke mean KURTOSIS microstructure changes white matter 1.5 TESLA magnetic resonance system neural REGENERATION
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Comparison of walking quality variables between incomplete spinal cord injury patients and healthy subjects by using a footscan plantar pressure system 预览
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作者 Xiang-Nan Yuan Wei-Di Liang +4 位作者 Feng-Hua Zhou Han-Ting Li Li-Xin Zhang Zhi-Qiang Zhang Jian-Jun Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第2期354-360,共7页
The main goal of spinal cord rehabilitation is to restore walking ability and improve walking quality after spinal cord injury (SCI). The spatiotemporal parameters of walking and the parameters of plantar pressure can... The main goal of spinal cord rehabilitation is to restore walking ability and improve walking quality after spinal cord injury (SCI). The spatiotemporal parameters of walking and the parameters of plantar pressure can be obtained using a plantar pressure analysis system. Previous studies have reported step asymmetry in patients with bilateral SCI. However, the asymmetry of other parameters in patients with SCI has not been reported. This was a prospective, cross-sectional study, which included 23 patients with SCI, aged 48.1 ± 14.5 years, and 28 healthy subjects, aged 47.1 ± 9.8 years. All subjects underwent bare foot walking on a plantar pressure measurement device to measure walking speed and spatiotemporal parameters. Compared with healthy subjects, SCI patients had slower walking speed, longer stride time and stance time, larger stance phase percentage, and shorter stride length. The peak pressures under the metatarsal heads and toe were lower in SCI patients than in healthy subjects. In the heel, regional impulse and the contact area percentage in SCI patients were higher than those in healthy subjects. The symmetry indexes of stance time, step length, maximum force, impulse and contact area were increased in SCI patients, indicating a decline in symmetry. The results confirm that the gait quality, including spatiotemporal variables and plantar pressure parameters, and symmetry index were lower in SCI patients compared with healthy subjects. Plantar pressure parameters and symmetry index could be sensitive quantitative parameters to improve gait quality of SCI patients. The protocols were approved by the Clinical Research Ethics Committee of Shengjing Hospital of China Medical University (approval No. 2015PS54J) on August 13, 2015. This trial was registered in the ISRCTN Registry (ISRCTN42544587) on August 22, 2018. Protocol version: 1.0. 展开更多
关键词 nerve REGENERATION spinal cord injury WALKING gait QUALITY PLANTAR PRESSURE SYSTEM PLANTAR PRESSURE distribution speed PLANTAR PRESSURE impulse contact area symmetry index neural REGENERATION
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Silencing Huwe1 reduces apoptosis of cortical neurons exposed to oxygen-glucose deprivation and reperfusion 预览
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作者 Guo-Qian He Wen-Ming Xu +3 位作者 Hui-Juan Liao Chuan Jiang Chang-Qing Li Wei Zhang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1977-1985,共9页
HECT, UBA and WWE domain-containing 1(Huwe1), an E3 ubiquitin ligase involved in the ubiquitin-proteasome system, is widely expressed in brain tissue. Huwe1 is involved in the turnover of numerous substrates, includin... HECT, UBA and WWE domain-containing 1(Huwe1), an E3 ubiquitin ligase involved in the ubiquitin-proteasome system, is widely expressed in brain tissue. Huwe1 is involved in the turnover of numerous substrates, including p53, Mcl-1, Cdc6 and N-myc, thereby playing a critical role in apoptosis and neurogenesis. However, the role of Huwe1 in brain ischemia and reperfusion injury remains unclear. Therefore, in this study, we investigated the role of Huwe1 in an in vitro model of ischemia and reperfusion injury. At 3 days in vitro, primary cortical neurons were transduced with a control or shRNA-Huwe1 lentiviral vector to silence expression of Huwe1. At 7 days in vitro, the cells were exposed to oxygen-glucose deprivation for 3 hours and reperfusion for 24 hours. To examine the role of the c-Jun N-terminal kinase(JNK)/p38 pathway, cortical neurons were pretreated with a JNK inhibitor(SP600125) or a p38 MAPK inhibitor(SB203508) for 30 minutes at 7 days in vitro, followed by ischemia and reperfusion. Neuronal apoptosis was assessed by TUNEL assay. Protein expression levels of JNK and p38 MAPK and of apoptosis-related proteins(p53, Gadd45 a, cleaved caspase-3, Bax and Bcl-2) were measured by western blot assay. Immunofluorescence labeling for cleaved caspase-3 was performed. We observed a significant increase in neuronal apoptosis and Huwe1 expression after ischemia and reperfusion. Treatment with the shRNA-Huwe1 lentiviral vector markedly decreased Huwe1 levels, and significantly decreased the number of TUNEL-positive cells after ischemia and reperfusion. The silencing vector also downregulated the pro-apoptotic proteins Bax and cleaved caspase-3, and upregulated the anti-apoptotic proteins Gadd45 a and Bcl-2. Silencing Huwe1 also significantly reduced p-JNK levels and increased p-p38 levels. Our findings show that downregulating Huwe1 affects the JNK and p38 MAPK signaling pathways as well as the expression of apoptosis-related genes to provide neuroprotection during ischemia and reperfusion. All animal experiments and 展开更多
关键词 nerve REGENERATION ischemic stroke oxygen-glucose DEPRIVATION and REPERFUSION ischemia/reperfusion cortical neuron ubiquitin proteasome system Huwe1 APOPTOSIS therapeutic targets CELL culture CELL death neural REGENERATION
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A novel large animal model of recurrent migraine established by repeated administration of inflammatory soup into the dura mater of the rhesus monkey 预览
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作者 Ning Chen Wei Su +4 位作者 Shu-Hui Cui Jian Guo Jia-Chuan Duan Hong-Xia Li Li He 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第1期100-106,共7页
关键词 动物模型 周期性 恒河猴 偏头痛 母亲 c-fos 管理 反常行为
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BEST系统变工况特性及控制方式研究
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作者 王雅倩 付亦葳 +1 位作者 张泽雄 殷江涛 《热能动力工程》 CAS CSCD 北大核心 2019年第5期42-48,共7页
为分析抽汽背压式汽轮机(Backpressure Extraction Steam Turbine,BEST)回热系统的变工况工作特性及保证小汽轮机与给水泵之间的功率匹配,提出采用小发电机、节流阀、补汽阀的3种控制策略,根据汽轮机、回热加热器等主要设备的变工况过... 为分析抽汽背压式汽轮机(Backpressure Extraction Steam Turbine,BEST)回热系统的变工况工作特性及保证小汽轮机与给水泵之间的功率匹配,提出采用小发电机、节流阀、补汽阀的3种控制策略,根据汽轮机、回热加热器等主要设备的变工况过程建立BEST系统的变工况数学模型,分析各负荷下BEST和给水泵系统的功率匹配特性和回热系统抽汽参数的变化规律。研究表明:小汽轮机与给水泵的功率差值随着负荷降低先增加后降低,最大值出现在约50%负荷左右;采用小发电机调节,BEST末级排汽流量与压力近似不变;采用节流阀调节,BEST末级排汽流量与压力随负荷降低而降低;采用补汽阀调节,BEST末级排汽压力与流量随负荷降低而升高;小发电机调节方式的热经济性最佳,相对于其他两种调节方式的热耗率明显偏低,节流调节方式存在节流损失,补汽阀调节在BEST末级排汽供汽中引入了大量经过再热的抽汽,提高了抽汽过热度,降低了系统效率。 展开更多
关键词 BEST系统 变工况 回热系统 汽轮机 给水泵
贡菊再生体系建立及羧苄青霉素浓度筛选 预览
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作者 徐亚男 任镘蓉 +3 位作者 岳圆圆 赵丽 廉美兰 高日 《延边大学农学学报》 2019年第3期41-45,共5页
以贡菊试管苗叶片为外植体,研究了影响不定芽分化和生根的因素,旨在建立贡菊高效的再生体系,同时探究了羧苄青霉素浓度对叶片不定芽分化的影响,为贡菊育种奠定基础。结果表明:在MS+BA 2.0 mg/L+NAA 0.6 mg/L的培养基中,贡菊试管苗叶龄为... 以贡菊试管苗叶片为外植体,研究了影响不定芽分化和生根的因素,旨在建立贡菊高效的再生体系,同时探究了羧苄青霉素浓度对叶片不定芽分化的影响,为贡菊育种奠定基础。结果表明:在MS+BA 2.0 mg/L+NAA 0.6 mg/L的培养基中,贡菊试管苗叶龄为25 d的叶片中愈伤组织诱导和不定芽分化率达到最高(88.4%),每个叶盘平均分化4.6个不定芽。当MS培养基中的IBA浓度为0.25 mg/L时,最适合贡菊试管苗根的生长,根长为4.5 cm,根数达15条,同时地上部茎叶生长也较好。低于250 mg/L的羧苄青霉素不影响不定芽愈伤组织中的诱导,但浓度高于500 mg/L时,抑制不定芽的分化。 展开更多
关键词 贡菊 再生体系 羧苄青霉素 不定芽 生长调节剂
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Mitophagy links oxidative stress conditions and neurodegenerative diseases 预览
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作者 Ulfuara Shefa Na Young Jeong +4 位作者 In Ok Song Hyung-Joo Chung Dokyoung Kim Junyang Jung Youngbuhm Huh 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期749-756,共8页
Mitophagy is activated by a number of stimuli,including hypoxia,energy stress,and increased oxidative phosphorylation activity.Mitophagy is associated with oxidative stress conditions and central neurodegenerative dis... Mitophagy is activated by a number of stimuli,including hypoxia,energy stress,and increased oxidative phosphorylation activity.Mitophagy is associated with oxidative stress conditions and central neurodegenerative diseases.Proper regulation of mitophagy is crucial for maintaining homeostasis;conversely,inadequate removal of mitochondria through mitophagy leads to the generation of oxidative species,including reactive oxygen species and reactive nitrogen species,resulting in various neurodegenerative diseases,such as Alzheimer’s disease,Parkinson’s disease,Huntington’s disease,and amyotrophic lateral sclerosis.These diseases are most prevalent in older adults whose bodies fail to maintain proper mitophagic functions to combat oxidative species.As mitophagy is essential for normal body function,by targeting mitophagic pathways we can improve these disease conditions.The search for effective remedies to treat these disease conditions is an ongoing process,which is why more studies are needed.Additionally,more relevant studies could help establish therapeutic conditions,which are currently in high demand.In this review,we discuss how mitophagy plays a significant role in homeostasis and how its dysregulation causes neurodegeneration.We also discuss how combating oxidative species and targeting mitophagy can help treat these neurodegenerative diseases. 展开更多
关键词 nerve regeneration MITOPHAGY central nervous system Alzheimer’s DISEASE Parkinson’s DISEASE Huntington’s DISEASE amyotrophic lateral SCLEROSIS oxidative SPECIES REACTIVE oxygen SPECIES REACTIVE nitrogen SPECIES
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文化识别性在历史街区更新中的提升策略--以上海市愚园路(定西路-江苏路)历史街区为例 预览
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作者 王与纯 沈则诚 《城市建筑》 2019年第16期173-182,共10页
城市历史街区由于其丰厚的人文遗存、浓郁的生活气息成了极具开发潜力的城市更新地段,而愚园路(定西路-江苏路)历史街区作为上海市规模较大、优秀历史建筑较多的城市保护地段,近几年的更新工作卓有成效。本文以愚园路的定西路至江苏路... 城市历史街区由于其丰厚的人文遗存、浓郁的生活气息成了极具开发潜力的城市更新地段,而愚园路(定西路-江苏路)历史街区作为上海市规模较大、优秀历史建筑较多的城市保护地段,近几年的更新工作卓有成效。本文以愚园路的定西路至江苏路街区为例,对该城市地段进行了空间权力、整体性、吸引力及文化脉络的分析,发现了其在现阶段更新中丧失了文化特色这一核心问题,并针对该问题提出文化识别性在该类型街区更新中的提升策略,主要内容包括社会生活方式、城市历史建筑及城市肌理的识别性提升。该策略能够充分发挥历史街区文化资源的价值,实现城市文化可持续发展。 展开更多
关键词 城市更新 历史文化风貌区 愚园路街区 文化资源 空间体系
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五莲杨(Populus wulianensis)再生体系建立及试管苗玻璃化的防治
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作者 王艳 吴丹 +6 位作者 刘立江 韩义 孙涛 王宁 解孝满 董昕 鲁仪增 《分子植物育种》 CSCD 北大核心 2019年第19期6434-6446,共13页
为开展特有树种五莲杨异地保存与离体保存、促进其保护利用,以其两年生春梢嫩芽为外植体建立组织再生体系。结果表明,五莲杨玻璃化发生率高达65.9%,且与基础培养基的类型显著相关;采用两步正交试验L9(34)方法探索了不同生长调节因子和... 为开展特有树种五莲杨异地保存与离体保存、促进其保护利用,以其两年生春梢嫩芽为外植体建立组织再生体系。结果表明,五莲杨玻璃化发生率高达65.9%,且与基础培养基的类型显著相关;采用两步正交试验L9(34)方法探索了不同生长调节因子和外源添加物对五莲杨试管苗玻璃化的影响,筛选出五莲杨初代培养基配方:1/2MS(NH4NO3)+0.05 mg/L NAA+0.4 mg/L 6-BA+7 g/L琼脂+30 g/L蔗糖,试管苗不定芽玻璃化发生率降低至5%以下,平均苗高5.32 cm;将基础培养基调整为MS,微调节NAA和6-BA浓度,筛选出五莲杨增殖培养基配方:MS+0.04 mg/L NAA+0.3 mg/L 6-BA+7 g/L琼脂+30 g/L蔗糖,试管苗不定芽增殖系数为15.35,平均苗高5.64 cm,无玻璃化试管苗,无菌苗茎叶粗壮且生长旺盛;调节NAA、IBA浓度及蔗糖配比,筛选出五莲杨生根培养基配方:1/2MS+0.05 mg/L NAA+15 g/L蔗糖+7.0 g/L琼脂粉,五莲杨生根率达92.0%,平均苗高为4.07 cm;幼苗经炼苗后进行移栽,成活率可达95%。本研究建立了五莲杨组织快繁体系,并通过正交实验的方法解决了试管苗玻璃化的问题,为五莲杨遗传转化及分子育种提供了理论依据。 展开更多
关键词 五莲杨(Populus wulianensis) 再生体系 玻璃化试管苗 1/2MS(NH4NO3)
假俭草(Eremochloa ophiuroides(Munro.)Hack)的高效组织培养再生体系的建立
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作者 马生健 鲁泽东 +1 位作者 曾富华 刘金祥 《分子植物育种》 CSCD 北大核心 2019年第19期6461-6468,共8页
本研究以假俭草(Eremochloa ophiuroides(Munro.)Hack)为材料,探讨了不同外植体、基本培养基类型、外源激素与固化剂种类对愈伤组织诱导的影响,并进行了三种不同外观状态的愈伤组织体细胞胚胎发生和内源激素含量测定及乙烯发生抑制剂对... 本研究以假俭草(Eremochloa ophiuroides(Munro.)Hack)为材料,探讨了不同外植体、基本培养基类型、外源激素与固化剂种类对愈伤组织诱导的影响,并进行了三种不同外观状态的愈伤组织体细胞胚胎发生和内源激素含量测定及乙烯发生抑制剂对愈伤组织分化长芽的影响研究。结果表明:含低浓度2,4-D的培养基上萌发的芽尖作外植体,诱导效果最佳;2,4-D进行愈伤组织诱导的最佳浓度为3.5 mg/L;基本培养基诱导率以N6最高;Phytagel与Agarose为效果最优的固化剂。体细胞胚胎研究表明初代淡黄色松软愈伤组织,其核小或无明显细胞核,以非胚性化细胞为主;部分区域开始呈现胚性愈伤组织,核大,胞质较浓,胚性化特征明显。内源激素测定赤霉素(GA3)只存在非胚性愈伤组织(non-embryonic callus,NEC)中;生长激素(IAA)只存在胚性愈伤组织(embryonic callus,EC);细胞分裂素(ZT)含量在三类愈伤组织中都较低。分化结果表明,1~5 mg/L的CoCl2和5~30 mg/L的AgNO3能提高假俭草愈伤组织分化长芽率,生根壮苗则在1/2MS+NAA0.5 mg/L易于长根。本研究通过建立详细的假俭草高效组织培养再生体系,为其后续转基因分子育种及用CRISPR研究其基因功能打下基础。 展开更多
关键词 假俭草(Eremochloa ophiuroides(Munro.)Hack) 组织培养 再生体系
Dendritic shrinkage after injury: a cellular killer or a necessity for axonal regeneration? 预览
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作者 An Beckers, Lieve Moons 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第8期1313-1316,共4页
Dendrites form an essential component of the neuronal circuit have been largely overlooked in regenerative research. Nevertheless, subtle changes in the dendritic arbors of neurons are one of the first stages of vario... Dendrites form an essential component of the neuronal circuit have been largely overlooked in regenerative research. Nevertheless, subtle changes in the dendritic arbors of neurons are one of the first stages of various neurodegenerative diseases, leading to dysfunctional neuronal networks and ultimately cellular death. Maintaining dendrites is therefore considered an essential neuroprotective strategy. This mini-review aims to discuss an intriguing hypothesis, which postulates that dendritic shrinkage is an important stimulant to boost axonal regeneration, and thus that preserving dendrites might not be the ideal therapeutic method to regain a full functional network upon central nervous system damage. Indeed, our study in zebrafish, a versatile animal model with robust regenerative capacity recently unraveled that dendritic retraction is evoked prior to axonal regrowth after optic nerve injury. Strikingly, inhibiting dendritic pruning upon damage perturbed axonal regeneration. This constraining effect of dendrites on axonal regrowth has sporadically been proposed in literature, as summarized in this short narrative. In addition, the review discusses a plausible underlying mechanism for the observed antagonistic axon-dendrite interplay, which is based on energy restriction inside neurons. Axonal injury indeed leads to a high local energy demand in which efficient axonal energy supply is fundamental to ensure regrowth. At the same time, axonal lesion is known to induce m让ochondrial depolarization, causing energy depletion in the axonal compartment of damaged neurons. Mitochondria, however, become mostly stationary after development, which has been proposed as a potential underlying reason for the low regenerative capacity of adult mammals. Per contra, upon reduced neuronal activity, mitochondrial mobility enhances. In this view, dendritic shrinkage after axonal injury in zebrafish could result in less synaptic input and hence, a release of mitochondria within the soma-dendrite compartment that then translocate 展开更多
关键词 AXONAL regeneration DENDRITIC remodeling RETINA central nervous system zebrafish MITOCHONDRIAL transport MITOCHONDRIAL dynamics energy supply
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马来甜龙竹种胚培养增殖芽芽尖再生体系建立 预览
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作者 诸葛菲 洪彬 +2 位作者 徐胤 沈琦超 林新春 《核农学报》 CAS CSCD 北大核心 2019年第12期2319-2327,共9页
为建立马来甜龙竹高效稳定的再生体系,本研究以马来甜龙竹种胚培养增殖芽的芽尖为试验材料,研究不同植物激素、有机添加物以及弱光处理对其再生体系建立的影响。结果表明,马来甜龙竹芽尖愈伤组织诱导较适宜培养基为添加0.5~1.0 mg·... 为建立马来甜龙竹高效稳定的再生体系,本研究以马来甜龙竹种胚培养增殖芽的芽尖为试验材料,研究不同植物激素、有机添加物以及弱光处理对其再生体系建立的影响。结果表明,马来甜龙竹芽尖愈伤组织诱导较适宜培养基为添加0.5~1.0 mg·L-1 NAA和3 mg·L-1 2,4-D的MS培养基,致密颗粒状愈伤组织诱导率达61.7%;较适宜的分化培养基为添加1 mg·L-1 6-BA、1 mg·L-1 KT和0.25 mg·L-1 NAA的MS培养基,10μmol·m-2·s-1弱光处理6 d,分化率达46.67%,再生苗伸长生长良好;较适宜的生根培养基为添加3 mg·L-1 IBA的1/2 MS培养基,生根率为55%,根较粗,其中部分根长有侧根。试管苗移植到混合基质中,成活率为83.33%。本研究初步建立了马来甜龙竹种胚培养增殖芽芽尖再生体系,为马来甜龙竹遗传转化体系的研究和应用奠定了基础。 展开更多
关键词 马来甜龙竹 种胚培养增殖芽芽尖 再生体系 组织细胞学观察
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可再生Fe3O4/CuFeS2/生物质复合降解柱对有机染料的处理性能 预览
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作者 张巨擘 孙晓晗 +5 位作者 高桥娇 王海鑫 梁大鑫 刘志明 韩光亭 姜伟 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2019年第3期425-430,共6页
采用具有单分散性、平均粒径分别为(200±0.5)nm和(6.5±0.5)nm的Fe3O4纳米微球和CuFeS2纳米晶与生物质废弃物复合制备出Fe3O4/CuFeS2/生物质复合降解柱.该降解柱能高效处理有机染料亚甲基蓝(MB)和罗丹明B(RhB),降解率超过90%.C... 采用具有单分散性、平均粒径分别为(200±0.5)nm和(6.5±0.5)nm的Fe3O4纳米微球和CuFeS2纳米晶与生物质废弃物复合制备出Fe3O4/CuFeS2/生物质复合降解柱.该降解柱能高效处理有机染料亚甲基蓝(MB)和罗丹明B(RhB),降解率超过90%.CuFeS2中的Cu+能显著促进芬顿反应,而Fe3O4较强的磁性有利于催化剂的回收.更重要的是,再生后的复合柱对有机染料的降解率没有明显降低,仍可达90%以上.此外,该降解柱可充分利用生物质废弃物,解决其回收利用的难题. 展开更多
关键词 复合降解柱 有机染料 再生 芬顿体系
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贵港报春苣苔的叶片组培与植株再生 预览
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作者 闫海霞 黄昌艳 +4 位作者 张自斌 崔学强 邓杰玲 关世凯 卜朝阳 《热带作物学报》 CSCD 北大核心 2019年第1期98-106,共9页
以贵港报春苣苔的叶片为外植体,研究不同培养基对其不定芽诱导和增殖、愈伤组织诱导与分化以及生根的影响。结果表明,外植体叶片以纵切为宜,不定芽诱导最适培养基为MS+6-BA 4.0 mg/L+IAA 1.5 mg/L,愈伤组织诱导最适培养基为MS+6-BA 3.0~... 以贵港报春苣苔的叶片为外植体,研究不同培养基对其不定芽诱导和增殖、愈伤组织诱导与分化以及生根的影响。结果表明,外植体叶片以纵切为宜,不定芽诱导最适培养基为MS+6-BA 4.0 mg/L+IAA 1.5 mg/L,愈伤组织诱导最适培养基为MS+6-BA 3.0~5.0 mg/L+2,4-D 0.5~1.0 mg/L,不定芽诱导率以及愈伤组织诱导率均为100.00%;愈伤在MS+KT 1.0 mg/L+NAA 0.2 mg/L+potato 30 g/L+banana 30 g/L+apple 20 g/L+coconut juice 100 mL/L 培养基上分化系数达12.64;不定芽在MS+ZT 1.0 mg/L+NAA 0.10 mg/L+potato 30 g/L+banana 30 g/L+apple 20 g/L+coconut juice 100 mL/L 培养基的增殖系数为8.55;不定芽在3/4 MS+NAA 0.01~0.05 mg/L+活性炭1.0~3.0 g/L 培养基上的生根率为100.00%。综上所述,叶片纵切后能通过不定芽途径以及愈伤组织途径建立贵港报春苣苔的组织培养技术体系,在该体系下不定芽增殖系数高、愈伤分化高,组培苗生根好。 展开更多
关键词 贵港报春苣苔 组织培养 再生体系 天然提取物
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苦瓜下胚轴的离体再生体系 预览
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作者 潘琼玉 田丽波 +4 位作者 商桑 邹凯茜 周萌萌 朱国鹏 曾丽萍 《中国瓜菜》 CAS 北大核心 2019年第5期22-27,共6页
为了建立高频的苦瓜离体再生体系,为苦瓜遗传转化提供技术基础,筛选了最适消毒方法和外植体类型。以MS培养基为基础培养基,添加不同浓度激素组合,筛选最适于苦瓜不定芽诱导、伸长和生根的培养基。最佳的消毒方法为:用水浸泡去壳的种子15... 为了建立高频的苦瓜离体再生体系,为苦瓜遗传转化提供技术基础,筛选了最适消毒方法和外植体类型。以MS培养基为基础培养基,添加不同浓度激素组合,筛选最适于苦瓜不定芽诱导、伸长和生根的培养基。最佳的消毒方法为:用水浸泡去壳的种子15min,在超净工作台中用75%酒精浸泡30s后再用4%NaClO浸泡6min,用无菌水冲洗4~5遍,污染率为0,发芽率为98%;最佳的愈伤组织诱导培养基为MS+2.5mg·L-16-BA+0.01mg·L-1IBA,愈伤组织密度为0.79g·cm-3,不定芽的分化率为39%,最佳外植体为下胚轴(12d),诱导率为41%;最佳的增殖培养基为MS+1.0mg·L-16-BA+0.005mg·L-1IBA;最佳的生根培养基为MS+0.1mg·L-1IBA,移栽存活率为74%。初步建立了苦瓜离体再生体系。 展开更多
关键词 苦瓜 再生体系 离体培养 愈伤组织
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转基因杜仲再生体系的优化 预览
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作者 王玲 董旋 +2 位作者 谭艾娟 赵德刚 赵懿琛 《种子》 北大核心 2019年第5期18-22,27共6页
为了提高杜仲不定芽的获得率及生根率,选用B5、MS和WPM 3种培养基,以杜仲下胚轴为外植体,利用农杆菌介导pGM 626-Act1-EuDIR3和pSH 737-35S-EuDIR1遗传转化杜仲,并将浸染后的各外植体分别接种于3种不同培养基上,观察记录各培养基上外植... 为了提高杜仲不定芽的获得率及生根率,选用B5、MS和WPM 3种培养基,以杜仲下胚轴为外植体,利用农杆菌介导pGM 626-Act1-EuDIR3和pSH 737-35S-EuDIR1遗传转化杜仲,并将浸染后的各外植体分别接种于3种不同培养基上,观察记录各培养基上外植体的生长情况,通过GUS染色检测和PCR验证统计得到阳性芽数。结果表明:转化质粒在B5、MS和WPM培养基上的平均出芽率分别为(14.79±6.20)%、(7.68±2.37)%和(4.05±0.64)%,相较于MS和WPM培养基,B5培养基上的愈伤组织生长旺盛,色泽鲜艳呈绿色,质地紧密脆嫩,平均出芽率显著高于另外2种培养基,但是WPM培养基的生根率显著高于另外2种培养基,WPM和B5培养基的生根率分别为53.85%和3.57%,在MS培养基上的阳性芽并未生根。因此,在杜仲遗传转化过程中,B5培养基更适合诱导不定芽,WPM培养基利于不定芽生根。本研究优化了转基因杜仲再生体系,为杜仲基因功能研究提供技术支持。 展开更多
关键词 转基因 杜仲 再生体系 培养基
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