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Comparison of the Roles of Lymphatic Vessels and Blood Vessels in the Recurrence of Pterygia: A Retrospective Study 认领
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作者 Wei Zhao Tao Wang +5 位作者 Jiezheng Yang Shiyi Yin Ting Wang Lei Zhong Lili Chen Shiqi Ling 《眼科学期刊(英文)》 2020年第2期142-153,共12页
Purpose: Increased conjunctival arteries and lymphangions accelerate the cellular immune response in recurrent pterygium, however, which plays a more important role warrants further investigation. The aim of the study... Purpose: Increased conjunctival arteries and lymphangions accelerate the cellular immune response in recurrent pterygium, however, which plays a more important role warrants further investigation. The aim of the study is to compare the roles of lymphatic and blood vessels in pterygium recurrence. Methods: Histological sections from 48 excised recurrent pterygia (including 14 Grade 1, 20 Grade 2, and 14 Grade 3 tissues) were examined. Histological sections from seven nasal epibulbar conjunctival segments served as normal controls. Blood and lymphatic vessels were evaluated and compared according to blood microvessel density (BMD), blood vascular area (BVA), lymphatic microvessel density (LMD), and lymph-vascular area (LVA). Furthermore, the following relationships were analyzed: LMD and pterygium recurrence time (RT), LVA and RT, BMD and RT, BVA and RT. Results: Compared to LVA, LMD, BVA, and BVD values in normal control tissues, these values were markedly enhanced in recurrent pterygia tissues. The LMD/BMD and LVA/BVA ratios were significant increased in Grade 2 and 3 recurrent pterygia, suggesting that development of conjunctival lymphangions was not proportional to blood vessel growth. LMD, LVA and BVA were significantly correlated with RT for all grades of pterygia, while BMD was the only factor correlated with RT in Grade 1 pterygia. Moreover, no significant correlation was found between BMD and RT in Grade 2 and 3 pterygia. Conclusion: Compared to blood vessels, lymphangions might have a greater impact on pterygium recurrence. 展开更多
关键词 RELATIONSHIP Hemangiogenesis LYMPHANGIOGENESIS Pterygia RECURRENCE
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片仔癀对LncRNA-ANRIL介导大肠癌淋巴管新生的影响 认领
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作者 逯遥 张敏 +3 位作者 刘洁 毛倩倩 曹治云 林久茂 《康复学报》 CSCD 2020年第5期387-394,共8页
目的:研究片仔癀(PZH)体外对长链非编码RNA-ANRIL(LncRNA-ANRIL)介导大肠癌淋巴管新生的抑制作用及其机制。方法:将体外培养人大肠癌细胞HCT-116用PZH进行干预,分为对照组和PZH干预组(0.25、0.50、0.75 mg/mL),采用CCK-8实验检测细胞活... 目的:研究片仔癀(PZH)体外对长链非编码RNA-ANRIL(LncRNA-ANRIL)介导大肠癌淋巴管新生的抑制作用及其机制。方法:将体外培养人大肠癌细胞HCT-116用PZH进行干预,分为对照组和PZH干预组(0.25、0.50、0.75 mg/mL),采用CCK-8实验检测细胞活力,用RT-qPCR检测LncRNA-ANRIL的表达,用Western blot法检测促淋巴管新生因子VEGF-C蛋白表达;用Lipofectamine RNAiMAX转染HCT-116细胞,分为Si-NC组和Si-ANRIL组,采用RT-qPCR法检测LncRNA-ANRIL的表达,在倒置显微镜下观察细胞形态变化,用CCK-8实验检测细胞活力,Western blot检测促淋巴管新生因子VEGF-C蛋白表达;分别以Si-NC、Si-ANRIL和Si-NC+PZH(0.25 mg/mL)干预HCT-116细胞并收集细胞培养上清液即肿瘤培养上清(TSNs)用于培养人淋巴内皮细胞(HLEC),采用CCK-8实验检测细胞活力,Transwell实验观察细胞迁移能力和细胞侵袭能力,管腔形成实验观察细胞管腔形成能力。结果:不同浓度PZH干预HCT-116细胞均可显著抑制其活力(P<0.01),下调LncRNA-ANRIL表达(P<0.01)和VEGF-C蛋白表达,呈浓度依赖性;在HCT-116细胞中沉默LncRNA-ANRIL的表达,与Si-NC组比较,Si-ANRIL组LncRNA-ANRIL表达显著降低(P<0.05),且可降低细胞密度、细胞活力(P<0.01)和VEGF-C蛋白表达;收集的TSNs上清干预HLEC后,与Si-NC组比较,Si-ANRIL和Si-NC+PZH(0.25 mg/mL)组可显著降低HLEC活力、迁移能力、侵袭能力和管腔形成能力(P<0.01)。结论:PZH可抑制大肠癌细胞LncRNA-ANRIL表达及其介导的大肠癌淋巴管新生,LncRNA-ANRIL是PZH抑制大肠癌淋巴管新生的作用靶点之一。 展开更多
关键词 大肠癌 片仔癀 人淋巴内皮细胞 LncRNA-ANRIL 淋巴管新生
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心脏淋巴管的分布、功能和病理意义 认领
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作者 王海杰 谭玉珍 《解剖学报》 CAS CSCD 北大核心 2020年第3期469-472,共4页
心脏淋巴引流对于维持心肌内微环境稳定起着重要作用。在心肌梗死和心力衰竭等疾病,淋巴管损伤或功能障碍可引起心脏淋巴水肿,从而导致心肌纤维化,加重炎症反应和严重影响心功能。近年来,人们对于心脏淋巴水肿与心脏疾病的相互关系以及... 心脏淋巴引流对于维持心肌内微环境稳定起着重要作用。在心肌梗死和心力衰竭等疾病,淋巴管损伤或功能障碍可引起心脏淋巴水肿,从而导致心肌纤维化,加重炎症反应和严重影响心功能。近年来,人们对于心脏淋巴水肿与心脏疾病的相互关系以及心脏淋巴管新生的生理、病理意义的研究非常重视。靶向刺激心脏淋巴管新生可作为缓解心脏淋巴水肿的可靠疗法。然而,刺激心脏淋巴管新生的优化策略有待深入研究。本文主要综述心脏淋巴管的分布和功能特点,讨论心脏淋巴水肿的病理作用,心脏淋巴管新生的机制以及促进心脏淋巴管新生的措施和临床意义。 展开更多
关键词 淋巴管 淋巴水肿 淋巴管新生
胆管癌相关淋巴管上皮细胞高表达因子对淋巴管生成的促进作用 认领
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作者 张雯 孙铭阳 +5 位作者 巫雪茹 朱明玉 李响 唐思敏 葛贤秀 缪林 《医学研究生学报》 CAS 北大核心 2020年第6期582-586,共5页
目的淋巴管上皮细胞(LECs)是胆管癌微环境中参与淋巴转移的重要环节。文中检测胆管癌细胞刺激后LECs分泌炎症因子及趋化因子的调变情况,观察高表达因子对淋巴管生成的影响。方法制备胆管癌细胞(RBE、HCCC9810)、胆管癌细胞条件培养基(C... 目的淋巴管上皮细胞(LECs)是胆管癌微环境中参与淋巴转移的重要环节。文中检测胆管癌细胞刺激后LECs分泌炎症因子及趋化因子的调变情况,观察高表达因子对淋巴管生成的影响。方法制备胆管癌细胞(RBE、HCCC9810)、胆管癌细胞条件培养基(CCM)刺激后的LECs、正常LECs的条件培养基,通过蛋白芯片检测各样品中炎症因子与趋化因子的分泌情况。确定在CCM刺激后LECs培养上清中显著调高的因子,通过ELISA检测,确定目标因子含量与时间的关系。实验分空白对照组:由DMEM和ECM按浓度1∶1配置;CCM组:培养基由第48小时收集的CCM和ECM按浓度1∶1配置;CCM+Anti⁃ENA⁃78组:在CCM组培养基制备基础上加入1μg/mL上皮中性粒细胞活化肽⁃78(ENA⁃78)中和抗体;Anti⁃ENA⁃78组:在空白对照组的基础上加入1μg/mL ENA⁃78中和抗体;ENA⁃78组:在空白对照组培养基制备基础上加入20 ng/mL的ENA⁃78活性蛋白;ENA⁃78+SB225002组:在ENA⁃78组培养基制备基础上加入100 nmol/L SB225002;SB225002组:在空白对照组培养基制备基础上加入100 nmol/L SB225002。通过细胞增殖和淋巴管成管实验探索目标因子与淋巴管生成的关系。结果ENA⁃78、IP⁃10、GCP⁃2、MCP⁃2、MCP⁃3、MIP⁃3a、HCC⁃1、Lymphotactin在CCM刺激后的LECs上清中表达增高,其中ENA⁃78在CCM⁃LECs中调变程度最显著。I⁃TAC、MIP⁃1d、IL⁃10、MIG、PDGF⁃BB、CXCL16因子呈现表达下调。ENA⁃78蛋白在RBE⁃LECs、HCCC9810⁃LECs上清中浓度[(1190.94±120.64、1650.38±103.76)pg/mL]较正常LECs[(354.03±32.50)pg/mL]中富集(P<0.01)。CCM组和CCM+Anti⁃ENA⁃78组LECs增殖(1.19±0.14、0.59±0.07)较对照组(0.31±0.03)显著增强(P<0.05);CCM+Anti⁃ENA⁃78组较Anti⁃ENA⁃78组的增殖明显增强(P<0.05);但CCM+Anti⁃ENA⁃78组增殖较CCM组有明显减弱(P<0.01)。CCM组和CCM+Anti⁃ENA⁃78组LECs淋巴管成管数(27.67±5.73、7.67±1.25)较对照组(3.00±0.82)显著增强 展开更多
关键词 胆管癌 淋巴管上皮细胞 上皮中性粒细胞活化肽⁃78 IL⁃8B 淋巴管生成
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清解扶正颗粒对体外淋巴管生成的抑制作用 认领
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作者 关建华 逯遥 +1 位作者 朱晓勤 林久茂 《实用中西医结合临床》 2020年第11期153-156,共4页
目的:探讨清解扶正颗粒对体外淋巴管内皮细胞新生淋巴管能力的影响。方法:将体外培养淋巴管内皮细胞分为对照组、实验组(清解扶正颗粒浓度:0.5、1.0、2.0 mg/ml),采用MTT、DAPI染色,划痕损伤修复实验,迁移实验和管腔形成实验分别检测清... 目的:探讨清解扶正颗粒对体外淋巴管内皮细胞新生淋巴管能力的影响。方法:将体外培养淋巴管内皮细胞分为对照组、实验组(清解扶正颗粒浓度:0.5、1.0、2.0 mg/ml),采用MTT、DAPI染色,划痕损伤修复实验,迁移实验和管腔形成实验分别检测清解扶正颗粒对细胞活力、细胞凋亡、细胞迁移及淋巴管生成能力的影响。结果:清解扶正颗粒可抑制淋巴管内皮细胞增殖,促进细胞凋亡,抑制划痕损伤修复、细胞迁移及淋巴管腔的生成。结论:清解扶正颗粒对体外淋巴管内皮细胞新生淋巴管能力有显著的抑制作用。 展开更多
关键词 清解扶正颗粒 人淋巴管内皮细胞 淋巴管生成 抑制作用
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TNFSF15对肿瘤脉管生成调控的临床意义及研究进展 认领
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作者 秦婷婷 李凯 《肿瘤防治研究》 CAS CSCD 2020年第7期567-570,共4页
肿瘤坏死因子超家族成员15(TNFSF15)主要是由血管内皮细胞特异分泌的细胞因子。作为死亡受体3(DR3)的配体,它参与调控VEGF/VEGFR信号通路,进而影响多种脉管细胞的生理及病理过程,例如抑制血管内皮细胞增殖、促进树突状细胞成熟、促进淋... 肿瘤坏死因子超家族成员15(TNFSF15)主要是由血管内皮细胞特异分泌的细胞因子。作为死亡受体3(DR3)的配体,它参与调控VEGF/VEGFR信号通路,进而影响多种脉管细胞的生理及病理过程,例如抑制血管内皮细胞增殖、促进树突状细胞成熟、促进淋巴内皮细胞增殖和迁移、防止血管渗漏、抑制血管生成等。临床研究数据表明TNFSF15启动子区域突变引起的TNFSF15高表达会增加罹患多种肿瘤的风险。然而,TNFSF15对血管和淋巴管生成的影响截然相反,其可通过结合血管内皮细胞表面受体DR3抑制肿瘤内血管生成而抑制肿瘤生长,但与淋巴内皮细胞表面受体DR3结合后反导致肿瘤内淋巴管生成而促进肿瘤淋巴转移。分析其不同生物效应对肿瘤脉管生成治疗有一定临床意义,本文仅就此作一综述。 展开更多
关键词 TNFSF15 肿瘤 血管生成 淋巴管生成 肿瘤转移
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Adipose-derived stem cell therapy shows promising results for secondary lymphedema 认领
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作者 Li-Ru Hu Jian Pan 《世界干细胞杂志:英文版(电子版)》 SCIE CAS 2020年第7期612-620,共9页
Lymphedema is mainly identified by progressive soft tissue swelling in impaired lymphatic system.Secondary lymphedema attributed to cancer therapy,parasite infection,and trauma remains a serious global disease.Patient... Lymphedema is mainly identified by progressive soft tissue swelling in impaired lymphatic system.Secondary lymphedema attributed to cancer therapy,parasite infection,and trauma remains a serious global disease.Patients with lymphedema suffer swelling,pain,and fatigue,with the dysfunction of the deformed extremities reducing the quality of life and increasing the risk of infection and lymphangiosarcoma.Adipose-derived stem cells(ADSCs)possess prominent regenerative potential to differentiate into multilineage cells,and produce various lymphangiogenic factors,making ADSC therapy a promising approach for lymphedema.The development of lymphedema consists of local inflammation,the fibrosis of lymphatic vessels,and the deposition of adipose fat.Existing animal models do not mimic the chronic inflammation environment,therefore suitable models are required in further studies.Some signal pathways and molecular mechanisms in physiological and pathological lymphagiogenesis remain unclear.In previous animal and human trials,ADSC therapy reduced edema in varying degrees.A larger number of trials with larger samples and longer follow-up periods are required to verify the efficiency and feasibility of ADSC therapy.ADSCs are of easy availability and immune exemption,making them a candidate for lymphedema treatment.Whether ADSCs enhance malignant characteristics or trigger the malignant change deserves further exploration and study before ADSC therapy can be made widely available. 展开更多
关键词 Secondary lymphedema Adipose-derived stem cells LYMPHANGIOGENESIS Stem cells Cell therapy Lymphatic regeneration
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癌相关成纤维细胞与食管鳞状细胞癌淋巴转移及淋巴管生成的相关性研究 认领
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作者 陈超 田翔宇 +3 位作者 邱森 王潼 韩俊雅 陈奎生 《食管疾病》 2020年第1期34-37,共4页
目的探讨食管鳞状细胞癌中癌相关成纤维细胞浸润与淋巴转移及淋巴管生成的关系。方法本研究中收集53例手术切除并病理确诊的食管鳞状细胞癌新鲜组织,25例距癌灶>5 cm的正常食管黏膜新鲜组织,并统计患者性别、年龄、病理级别、浸润深... 目的探讨食管鳞状细胞癌中癌相关成纤维细胞浸润与淋巴转移及淋巴管生成的关系。方法本研究中收集53例手术切除并病理确诊的食管鳞状细胞癌新鲜组织,25例距癌灶>5 cm的正常食管黏膜新鲜组织,并统计患者性别、年龄、病理级别、浸润深度、淋巴结是否转移等临床病理资料,应用免疫组织化学染色法检测癌相关成纤维细胞标记蛋白α-SMA以及淋巴管内皮细胞标记蛋白D2-40。采用SPSS 21.0统计学软件对数据进行分析。结果食管鳞状细胞癌组织中癌相关成纤维细胞数量与微淋巴管密度均高于正常食管黏膜组织(P<0.05)。食管鳞状细胞癌组织中癌相关成纤维细胞数量与临床分期、浸润深度、肿瘤淋巴结转移密切相关(P<0.05),与性别、年龄无关。食管鳞状细胞癌组织中癌相关成纤维细胞数量与微淋巴管密度呈正相关(P<0.05)。结论癌相关成纤维细胞可能通过促进淋巴管生成参与食管鳞状细胞癌的浸润和转移,这可能为食管鳞状细胞癌的早期预防和靶向治疗提供新方向。 展开更多
关键词 癌相关成纤维细胞 食管鳞状细胞癌 淋巴转移 淋巴管生成
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诱导型一氧化氮合成酶在胃腺癌表达特点及与淋巴管形成的关系 认领
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作者 王嘉鑫 闫志军 +4 位作者 石安华 刘宥苡 吕煜 陈芸 代佑果 《医学研究生学报》 CAS 北大核心 2020年第9期937-941,共5页
目的诱导型一氧化氮合成酶(iNOS)表达与胃癌淋巴管形成及淋巴结转移的关系研究较少。文中旨在探讨胃腺癌组织中iNOS的表达,及与淋巴管形成的相关性。方法收集2017年7月至2018年12月昆明医科大学第三附属医院腹部外科78例胃腺癌并行胃腺... 目的诱导型一氧化氮合成酶(iNOS)表达与胃癌淋巴管形成及淋巴结转移的关系研究较少。文中旨在探讨胃腺癌组织中iNOS的表达,及与淋巴管形成的相关性。方法收集2017年7月至2018年12月昆明医科大学第三附属医院腹部外科78例胃腺癌并行胃腺癌根治术患者的癌组织及癌旁组织标本。采用免疫组化法检测iNOS和D2-40蛋白的表达,分析iNOS与D2-40蛋白表达的相关性,分析iNOS表达与胃腺癌临床病理特征的关系。结果胃腺癌组织中iNOS阳性表达率为64.10%(50/78),D2-40阳性表达率为73.08%(57/78)。胃腺癌组织的iNOS、D2-40阳性表达率明显高于癌旁组织(P<0.05)。胃腺癌组织中iNOS和D2-40的表达呈显著正相关(rs=0.532,P=0.001)。胃腺癌组织中iNOS表达与肿瘤分化程度有关,低分化腺癌阳性表达率较高中分化明显升高(72% vs 28%,P=O.CMl),iNOS阳性表达率随着临床T分期和N分期越晚越高(P<0.05)。结论iNOS表达在胃癌的淋巴管的形成中起重要作用,iNOS蛋白具有潜在靶向治疗的价值。 展开更多
关键词 胃腺癌 诱导型一氧化氮合酶 D2-40 淋巴管形成
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结直肠癌肿瘤中心区和边缘区淋巴管生成的临床意义 认领
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作者 张彦斌 刘月 +3 位作者 张辉 黄红艳 李莎 任军 《中华普通外科杂志》 CSCD 北大核心 2020年第8期612-615,共4页
目的 探讨肿瘤中心区和边缘区淋巴管生成与结直肠癌发展及预后的关系.方法 收集120例结直肠癌肿瘤标本,以淋巴管特异性标志物podoplanin抗体进行免疫组化染色,分别检测肿瘤中心区淋巴管密度和边缘区淋巴管密度,以了解结直肠癌不同区域... 目的 探讨肿瘤中心区和边缘区淋巴管生成与结直肠癌发展及预后的关系.方法 收集120例结直肠癌肿瘤标本,以淋巴管特异性标志物podoplanin抗体进行免疫组化染色,分别检测肿瘤中心区淋巴管密度和边缘区淋巴管密度,以了解结直肠癌不同区域的淋巴管生成情况,同时分析肿瘤不同区域淋巴管密度与结直肠癌患者的临床病理特征及预后关系.结果 与肿瘤边缘区淋巴管相比,肿瘤中心区淋巴管通常表现为体积更小的闭塞状和不规则状.结直肠癌肿瘤中心区淋巴管密度与肿瘤大小(t=2.673,P=0.009)、组织学分级(t=-2.296,P=0.023)及患者的预后(x^2=4.386,P=0.036)均有关,而肿瘤边缘区淋巴管密度与淋巴结转移(t=-4.053,P<0.001)、肿瘤分期(t=4.740,P=0.004)及患者的预后(x^2=5.806,P=0.016)均有关.结论 肿瘤边缘区淋巴管生成在结直肠癌淋巴结转移中发挥重要作用;肿瘤中心区淋巴管生成与结直肠癌肿瘤生长及分化密切相关;肿瘤边缘区和中心区淋巴管生成对于结直肠癌的发展及预后均具有重要影响. 展开更多
关键词 结直肠肿瘤 淋巴管生成 预后
Anlotinib suppresses lymphangiogenesis and lymphatic metastasis in lung adenocarcinoma through a process potentially involving VEGFR-3 signaling 认领
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作者 Tingting Qin Zhujun Liu +5 位作者 Jing Wang Junling Xia Shaochuan Liu Yanan Jia Hailin Liu Kai Li 《癌症生物学与医学:英文版》 SCIE CAS CSCD 2020年第3期753-767,共15页
Objective:Lymphatic metastasis is one of the leading causes of malignancy dispersion in various types of cancer.However,few anti-lymphangiogenic drugs have been approved for clinical use to date.Therefore,new therapie... Objective:Lymphatic metastasis is one of the leading causes of malignancy dispersion in various types of cancer.However,few anti-lymphangiogenic drugs have been approved for clinical use to date.Therefore,new therapies to block lymphangiogenesis are urgently required.Methods:Immunohistochemistry,immunofluorescence,Western blot,migration assays,and lymphangiogenesis and lymphatic metastasis assays were used.Results:Anlotinib,a receptor tyrosine kinase inhibitor,suppressed the rate of new metastatic lesions(31.82%in the placebo arm and 18.18%in the anlotinib arm)in patients with advanced lung adenocarcinoma who were enrolled in our ALTER-0303 study.D2-40+-lymphatic vessel density was strongly correlated with disease stage,metastasis,and poor prognosis in 144 Chinese patients with lung adenocarcinoma.In mice bearing A549EGFP tumors,tumor lymphatic vessel density,tumor cell migration to lymph nodes,and the number of distant metastatic lesions were lower in the anlotinib group than in the controls.Anlotinib inhibited the growth and migration of human lymphatic endothelial cells(hLECs)and lymphangiogenesisin vitro andin vivo.Treatment of hLECs with anlotinib downregulated phosphorylated vascular endothelial growth factor receptor 3(VEGFR-3).Conclusions:Anlotinib inhibits lymphangiogenesis and lymphatic metastasis,probably through inactivating VEGFR-3 phosphorylation.The results indicate that anlotinib may be beneficial for treatment in avoiding lymphangiogenesis and distant lymphatic metastasis in lung adenocarcinoma.(Trial registration:ALTER0303;NCT02388919;March 17,2015.) 展开更多
关键词 Anlotinib VEGFR-3 dephosphorylation LYMPHANGIOGENESIS lymph node metastasis lung adenocarcinoma
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LRG1 promotes corneal angiogenesis and lymphangiogenesis in a corneal alkali burn mouse model 认领
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作者 Shan Song Jun Cheng +3 位作者 Bing-Jie Yu Li Zhou Hai-Feng Xu Ling-Ling Yang 《国际眼科杂志:英文版》 SCIE CAS 2020年第3期365-373,共9页
AIM:To investigate the potential effect and mechanism of leucine-richα-2-glycoprotein-1(LRG1)on corneal angiogenesis and lymphangiogenesis.METHODS:Corneal neovascularization and lymphatics were induced by establishin... AIM:To investigate the potential effect and mechanism of leucine-richα-2-glycoprotein-1(LRG1)on corneal angiogenesis and lymphangiogenesis.METHODS:Corneal neovascularization and lymphatics were induced by establishing alkali burn mouse model.Immunofluorescence staining was performed to detect the location of LRG1 in cornea tissues and to verify the source of LRG1-positive cells.Corneal whole-mount staining for CD31(a panendothelial cell marker)and lymphatic endothelial hyluronan receptor-1(LYVE-1;lymphatic marker)was performed to detect the growth of blood and lymphatic vessels after local application of exogenous LRG1 protein or LRG1 si RNA.In addition,expressions of the proangiogenic vascular endothelial growth factor(VEGF)related proteins were detected using Western blot analysis.RESULTS:LRG1 was dramatically increased in alkali burned corneal stroma in both the limbal and central areas.LRG1-positive cells in the corneal stroma were mainly derived from Vimentin-positive cells.Local application ofexogenous LRG1 protein not only aggravated angiogenesis but also lymphangiogenesis significantly(P<0.01).LRG1 group upregulated the levels of VEGF and the vascular endothelial growth factor receptor(VEGFR)family when compared with the phosphate-buffered saline(PBS)control group.We also found that LRG1-specific si RNA could suppress corneal angiogenesis and lymphangiogenesis when compared with the scramble si RNA-treated group(P<0.01).CONCLUSION:LRG1 can facilitate corneal angiogenesis and lymphangiogenesis through heightening the stromal expression of VEGF-A,B,C,D and VEGFR-1,2,3;LRG1-specific si RNA can suppress corneal angiogenesis and lymphangiogenesis in corneal alkali burn mice. 展开更多
关键词 leucine-richα-2-glycoprotein-1 ANGIOGENESIS LYMPHANGIOGENESIS CORNEA alkali BURN vascular endothelial growth factor
Application and prospect of adipose stem cell transplantation in treating lymphedema 认领
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作者 Zhu-Jun Li Elan Yang +4 位作者 Yun-Zhu Li Zheng-Yun Liang Jiu-Zuo Huang Nan-Ze Yu Xiao Long 《世界干细胞杂志:英文版(电子版)》 SCIE CAS 2020年第7期676-687,共12页
BACKGROUND Lymphedema is a chronic,debilitating and incurable disease that affects 0.13%-2%of the global population.Emerging evidence indicates that adipose-derived stem cells(ADSCs)might serve as suitable seed cells ... BACKGROUND Lymphedema is a chronic,debilitating and incurable disease that affects 0.13%-2%of the global population.Emerging evidence indicates that adipose-derived stem cells(ADSCs)might serve as suitable seed cells for lymphatic tissue engineering and lymphedema therapy.AIM To summarize applications of ADSCs for treating lymphedema in both animal studies and clinical trials.METHODS A systematic search was performed on four databases-PubMed,Clinicaltrials.gov,the evidence-based Cochrane Library,and OVID-using the following search string:(“lymphedema”or“lymphoedema”or“lymphangiogenesis”)and(“adipose-derived stem cells”or“adipose-derived stromal cells”or“adipose-derived regenerative cells”).A manual search was performed by skimming the references of relevant studies.Animal studies and clinical trials using adipose-derived cells for the treatment of any kind of lymphedema were included.RESULTS A total of eight research articles published before November 2019 were included for this analysis.Five articles focused on animal studies and another three focused on clinical trials.ADSC transplantation therapy was demonstrated to be effective against lymphedema in all studies.The animal studies found that coadministration of ADSCs and controlled-release vascular endothelial growth factor-C or platelet-rich plasma could improve the effectiveness of ADSC therapy.Three sequential clinical trials were conducted on breast cancer-related lymphedema patients,and all showed favorable results.CONCLUSION ADSC-based therapy is a promising option for treating lymphedema.Large-scale,multicenter randomized controlled trials are needed to develop more effective and durable therapeutic strategies. 展开更多
关键词 LYMPHEDEMA Adipose-derived stem cells Animal model Clinical trial Vascular endothelial growth factor-C LYMPHANGIOGENESIS
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Gut Lymphangiopathy: Adding Fuel to the Fire in Chronic Liver Disease 认领
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作者 Luis Tresierra Maria-Angeles Aller +2 位作者 Isabel Prieto Luis Santamaria Jaime Arias 《生命科学与技术进展(英文)》 2019年第10期305-319,共15页
The splanchnic inflammation inchronic liver disease increases intestinal angiogenesis. In the current study our aim was demonstrating that the small bowel lymphangiogenesis is associated with angiogenesis in chronic c... The splanchnic inflammation inchronic liver disease increases intestinal angiogenesis. In the current study our aim was demonstrating that the small bowel lymphangiogenesis is associated with angiogenesis in chronic cholestasis in the rat. A stereological study of the lymphatic microcirculation in the small intestine was performed in cholestatic rats. Portal enteropathy in cholestasis increases lymphatic microvessels in the mucosa and submucosa layers. Thus, the lymphatic microvessel volume fraction was superior (p < 0.001) in the mucosa (0.16 ± 0.01) and submucosa (0.16 ± 0.01), in regard to the muscle layer 0.015 ± 0.01. The lymphatic microvessel length density was higher in the mucosa (76.89 ± 2.86 mm-2;p -2;p < 0.01), in relationship to the muscle layer (5.04 ± 2.92 mm-2). These alterations predominate in the duodenum (volume fraction: 0.10 ± 0.01 and length density: 33.55 ± 5.98 mm-2) and ileum (volume fraction: 0.16 ± 0.01 and length density: 38.62 ± 6.07 mm-2). This study demonstrates the predominance of an increased lymphangiogenic response in both end sides of the small bowel associated with chronic liver disease. Since the porto-systemic venous collateral circulation in the chronic liver insufficiency is developed in the ends of the gastrointestinal tract, the excessive duodeno-ileal lymphangiogenesis could suggest the development of amesenteric-systemic lymphatic bypass in the chronic portal hypertension. 展开更多
关键词 CHRONIC Liver Disease LYMPHANGIOGENESIS MICROSURGERY COLLATERAL LymphaticCirculation
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人参皂苷Rg3通过TGF-β1/ERK信号通路调控原位荷瘤人肺癌裸鼠的淋巴管生成的机制 认领
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作者 陶涛 汪国文 +1 位作者 李其才 黎传奎 《中国比较医学杂志》 CAS 北大核心 2019年第11期34-40,共7页
目的 探究人参皂苷Rg3(GS-Rg3)通过转化生长因子-β1(TGF-β1)/细胞外调节蛋白激酶(ERK)信号通路调控原位荷瘤人肺癌裸鼠淋巴管生成的机制。方法 60只裸鼠随机分为3组,即模型组、GS-Rg3组和ERK抑制剂(SCH772984)组。GS-Rg3组和SCH77298... 目的 探究人参皂苷Rg3(GS-Rg3)通过转化生长因子-β1(TGF-β1)/细胞外调节蛋白激酶(ERK)信号通路调控原位荷瘤人肺癌裸鼠淋巴管生成的机制。方法 60只裸鼠随机分为3组,即模型组、GS-Rg3组和ERK抑制剂(SCH772984)组。GS-Rg3组和SCH772984组建立人肺癌裸鼠原位移植模型,GS-Rg3组小鼠使用GSRg3灌胃,SCH772984组小鼠腹腔注射ERK抑制剂SCH772984。观察各组小鼠建模和淋巴转移情况。并分析各组肿瘤组织中淋巴管生成情况、TGF-β1/ERK信号通路以及血管内皮生长因子(VEGF)表达情况。结果 HE染色病理学检查证实肺癌以及肺癌淋巴转移。GS-Rg3组和SCH772984组小鼠出现淋巴转移的比例、肿瘤体积和肿瘤质量均显著低于模型组,GS-Rg3组和SCH772984组无显著差异。使用podoplanin蛋白标记淋巴管,GS-Rg3组和SCH772984组podoplanin蛋白表达水平和淋巴管相对密度显著低于模型组,GS-Rg3组和SCH772984组无显著差异。GS-Rg3和SCH772984可以显著抑制TGF-β1/ERK通路的激活。GS-Rg3组和SCH772984组的VEGF-C、VEGF-3表达水平较模型组低,GS-Rg3组和SCH772984组无显著差异。结论 GS-Rg3可以通过下调TGF-β1/ERK信号通路的转导水平抑制VEGF-C、VEGF-3蛋白的表达,从而抑制淋巴管的生成降低肺癌的淋巴转移率,其作用水平与SCH772984相似。 展开更多
关键词 肺癌 人参皂苷RG3 转化生长因子-β1/细胞外调节蛋白激酶 淋巴管生成 血管内皮生长因子 裸鼠
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去甲斑蝥素对人真皮淋巴管内皮细胞体外三维培养淋巴管生成的影响及其机制 认领
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作者 徐永良 孙平 +1 位作者 李明秋 赵微 《世界复合医学》 2019年第7期116-119,共4页
目的观察去甲斑蝥素(NCTD)对人真皮淋巴管内皮细胞(HDLEC)体外三维培养淋巴管生成的影响,并分析其机制。方法构建HDLEC体外培养淋巴管生成模型,遵循随机原则分为NCTD组与空白对照组,于NCTD组加入1/3IC50NCTD40nM,持续反应24 h,对两组淋... 目的观察去甲斑蝥素(NCTD)对人真皮淋巴管内皮细胞(HDLEC)体外三维培养淋巴管生成的影响,并分析其机制。方法构建HDLEC体外培养淋巴管生成模型,遵循随机原则分为NCTD组与空白对照组,于NCTD组加入1/3IC50NCTD40nM,持续反应24 h,对两组淋巴管生成的形态变化情况以及淋巴管生成因子蛋白、基因表达情况予以比较。结果 NCTD组呈现出明显的HDLEC细胞形态改变,仅有少部分表现为管状结构,随着时间的延长,细胞表现为浮起、变圆形态直至死亡。空白对照组淋巴管生成数量为(67.29±5.36)个,NCTD组淋巴管生成数量为(10.92±2.35)个,差异有统计学意义(t=23.552,P<0.05);经过免疫细胞化学染色及Western blot试验,可以发现NCTD组三维培养淋巴管中的VEGF-C以及VEGF-D蛋白呈现出明显的下降趋势,分别为(2.43±0.12)%、(16.09±1.24)%,且对VEGF-C、VEGF-D以及VEGFR-3基因表达呈现出降低趋势,差异有统计学意义(t=8.493、10.275、6.432,P<0.05)。结论去甲斑蝥素能够对淋巴管生成因子VEGF-C以及VEGF-D蛋白及基因表达进行下调,进而起到抗淋巴管生成作用。 展开更多
关键词 去甲斑蝥素 淋巴管内皮细胞 三维培养 淋巴管生成 影响机制
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疏肝健脾饮对乳腺癌癌前病变裸鼠淋巴管生成的影响研究 认领 被引量:1
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作者 李斐斐 刘晓菲 +2 位作者 张洋 王圆圆 韩笑 《现代中西医结合杂志》 CAS 2019年第21期2292-2296,共5页
目的研究疏肝健脾饮对乳腺癌癌前病变裸鼠淋巴管生成途径COX-2/VEGF通路中血管内皮生长因子-C(VEGF-C)、血管内皮生长因子受体3(VEGFR-3)、环氧合酶-2(COX-2)蛋白及mRNA表达的影响,探讨疏肝健脾饮对乳腺癌癌前病变淋巴管生成的干预作用... 目的研究疏肝健脾饮对乳腺癌癌前病变裸鼠淋巴管生成途径COX-2/VEGF通路中血管内皮生长因子-C(VEGF-C)、血管内皮生长因子受体3(VEGFR-3)、环氧合酶-2(COX-2)蛋白及mRNA表达的影响,探讨疏肝健脾饮对乳腺癌癌前病变淋巴管生成的干预作用机制,寻找中药复方抑制淋巴管生成的作用靶点。方法取120只BALB/c-nudemice裸鼠,采用乳腺原位移植MCF-10AT细胞株方法建立癌前病变模型,建模成功后随机分成6组,每组20只。模型对照组裸鼠予以0.9%氯化钠溶液灌胃,疏肝健脾饮组、疏肝理气组、健脾化痰组、活血化瘀组分别予以相应中药制剂灌胃,均1次/d;他莫昔芬组给予他莫昔芬腹腔注射,每周2次。各组均连续干预9周。至第9周周末处死各组裸鼠,分别采用Western-blot、RT-PCR技术检测肿瘤组织中VEGF-C、VEGFR-3、COX-2蛋白及mRNA表达情况。结果疏肝健脾饮组、疏肝理气组、活血化瘀组、他莫昔芬组VEGF-C蛋白及mRNA相对表达量均明显低于模型对照组(P均<0.05),且疏肝健脾饮组均明显低于活血化瘀组(P均<0.05)。疏肝健脾饮组、健脾化痰组、他莫昔芬组VEGFR-3蛋白相对表达量均明显低于模型对照组(P均<0.05),且疏肝健脾饮组及他莫昔芬组均明显低于疏肝理气组(P均<0.05);疏肝健脾饮组、疏肝理气组、他莫昔芬组COX-2蛋白相对表达量均明显低于模型对照组(P均<0.05),且疏肝健脾饮组及他莫昔芬组均明显低于健脾化痰组及活血化瘀组(P均<0.05)。疏肝健脾饮组、疏肝理气组、他莫昔芬组VEGFR-3mRNA相对表达量均明显低于模型对照组(P均<0.05),且疏肝健脾饮组和他莫昔芬组明显低于活血化瘀组(P均<0.05);疏肝健脾饮组、他莫昔芬组COX-2mRNA相对表达量均明显低于模型对照组和疏肝理气组(P均<0.05)。疏肝健脾饮组与他莫昔芬组VEGF-C、VEGFR-3、COX-2蛋白及mRNA相对表达量比较差异均无统计学意义(P均>0.05)。结论疏肝健� 展开更多
关键词 疏肝健脾饮 乳腺癌癌前病变 淋巴管生成 蛋白表达 基因表达
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Relationship between tumor lymphangiogenesis and tumor metastasis 认领
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作者 Ya-Nan Man Rui Cao Xiong-Zhi Wu 《肿瘤微环境研究》 2019年第1期14-22,共9页
Objective:To summarize the research progress on the relationship between tumor lymphangiogenesis and tumor metastasis.Methods:PubMed and China National Knowledge Infrastructure(CNKI)database were used to search for re... Objective:To summarize the research progress on the relationship between tumor lymphangiogenesis and tumor metastasis.Methods:PubMed and China National Knowledge Infrastructure(CNKI)database were used to search for related literatures with the keywords of"tumor,lymphangiogenesis and lymphatic metastasis".Inclusion criteria:(1)the morphology and function of tumor lymphatic vessels;(2)the markers of lymphatic vessels;(3)the mechanism of tumor lymphangiogenesis;(4)the molecular regulation mechanism of tumor lymphangiogenesis.Results:The lymphatic vessel wall is only composed of endothelial cells,and the basement membrane is discontinuous with high permeability.The markers of lymphatic vessels include VEGFR-3,LYVE-1,podoplanin and thehomeoboxgene Prox-1,which facilitate the research on the function of lymphatic vessels in tumor.The mechanism of tumor lymphangiogenesis is very complicated,and there is no conclusion yet.It is believed that the tumor lymphangiogenesis is mainly derived from the existing lymphatic endothelial cells in the tissue.During the process of tumor lymphangiogenesis,many factors,such as VEGF-C,VEGF-D,VEGF-A,bFGF,PDGF-BB,Ang-2,NO and COX-2,are directly or indirectly involved.However,most studies believe that VEGF-C and VEGF-D are the most important regulators of tumor lymphangiogenesis.Conclusion:Tumor lymphangiogenesis is closely related to tumor lymphatic metastasis.Inhibition of tumor lymphangiogenesis can open a new way for tumor treatment. 展开更多
关键词 LYMPHANGIOGENESIS TUMOR METASTASIS BIOMARKER MOLECULAR mechanism
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高盐对血管内皮生长因子受体-3^+巨噬细胞表型及功能的影响 认领
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作者 余芳芳 杨国红 +5 位作者 李铭 陶燕燕 王秀娟 牛秀珑 李玉明 赵季红 《天津医药》 CAS 北大核心 2018年第12期1257-1262,1369共7页
目的观察高盐对血管内皮生长因子受体(VEGFR)-3^+巨噬细胞表型、淋巴管内皮细胞特性及功能的影响。方法利用流式分选将小鼠RAW264.7巨噬细胞中VEGFR-3^+亚群分选出来,分为Control组、低盐组(LS组,20mmol/LNaCl)和高盐组(HS组,40mmol/LNa... 目的观察高盐对血管内皮生长因子受体(VEGFR)-3^+巨噬细胞表型、淋巴管内皮细胞特性及功能的影响。方法利用流式分选将小鼠RAW264.7巨噬细胞中VEGFR-3^+亚群分选出来,分为Control组、低盐组(LS组,20mmol/LNaCl)和高盐组(HS组,40mmol/LNaCl)。利用CCK-8法观察不同组VEGFR-3^+细胞活性;Real-timePCR检测NaCl干预后VEGFR-3^+巨噬细胞表型变化及淋巴管内皮细胞标志物mRNA表达水平;Transwell实验检测各组细胞的迁移功能,流式细胞术检测不同组细胞的吞噬能力。结果与Control组相比,高盐干预可以使VEGFR-3^+巨噬细胞的白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α、CC类趋化因子配体(CCL)2、血管内皮生长因子(VEGF)-C及张力应答性增强子结合蛋白(TonEBP)mRNA表达水平上调(P<0.05);HS组在高盐干预24、48h后细胞活性均显著低于LS组(P<0.05);同时,HS组细胞迁移能力及细胞的吞噬能力与Control组相比显著增强,差异均有统计学意义(P<0.05)。结论高盐可使VEGFR-3^+巨噬细胞向M1型巨噬细胞偏移并表现出促淋巴管生成的特性,其迁移及吞噬能力显著增强,为进一步研究该亚群与淋巴管生成及心血管疾病的关系提供了依据。 展开更多
关键词 巨噬细胞 免疫表型分型 淋巴管生成 血管内皮生长因子受体3 高盐
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动态增强MRI检查对肺癌病灶内细胞增殖、血管和淋巴管新生的评估价值 认领
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作者 曹参 蔡丽 《海南医学院学报》 CAS 2018年第3期365-368,372共5页
目的:探讨动态增强MRI检查对肺癌病灶内细胞增殖、血管和淋巴管新生的评估价值。方法:选择在本院接受手术治疗的肺部小结节患者519例,根据术后病理结果分为NSCLC组410例、良性病变组109例。对比两组术前病灶动态增强MRI参数水平,病灶组... 目的:探讨动态增强MRI检查对肺癌病灶内细胞增殖、血管和淋巴管新生的评估价值。方法:选择在本院接受手术治疗的肺部小结节患者519例,根据术后病理结果分为NSCLC组410例、良性病变组109例。对比两组术前病灶动态增强MRI参数水平,病灶组织中增殖基因、血管新生相关基因、淋巴管新生相关基因表达量的差异。采用Pearson检验评估NSCLC患者病灶动态增强MRI参数水平与肿瘤细胞恶性程度的相关关系。结果:NSCLC组患者病灶动态增强MRI参数Ktrans、Kep水平高于良性病变组;NSCLC组病灶组织中增殖基因Axin、FHIT mRNA的表达量低于良性病变组,RACK1、HMGN5、MIF mRNA的表达量高于良性病变组;NSCLC组病灶组织中血管新生相关基因VEGF、COX 2、EGFR、MACC1 mRNA的表达量高于良性病变组;NSCLC组病灶组织中淋巴管新生相关基因Akt1、HIF 1α、Prox 1、PTTG mRNA的表达量高于良性病变组。相关性分析发现,NSCLC患者动态增强MRI参数Ktrans、Kep水平与病灶内细胞增殖、血管和淋巴管新生程度直接相关。结论:NSCLC患者病灶组织动态增强MRI参数水平异常改变,具体水平与肿瘤细胞恶性程度密切相关。 展开更多
关键词 肺癌 动态增强MRI 增殖基因 血管新生 淋巴管新生
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