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Immunolocalization of a Normal Wood Specific Pectin Methylesterase (CoPME) and Quantification of PME Gene Expression in Differentiating Xylem of <i>Chamaecyparis obtusa</i> 预览
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作者 Akinori Ota Masato Yoshida +3 位作者 Saori Sato Hideto Hiraide Miyuki Matsuo-Ueda Hiroyuki Yamamoto 《美国植物学期刊(英文)》 2019年第11期1949-1968,共20页
The transverse section of compression wood tracheids has a circular shape and intercellular spaces. The cause has not been determined yet;however, we hypothesized that peeling of the cell wall adhesion would cause cel... The transverse section of compression wood tracheids has a circular shape and intercellular spaces. The cause has not been determined yet;however, we hypothesized that peeling of the cell wall adhesion would cause cellular intervals, resulting in circularity of the transverse section of tracheids. Homogalacturonan, a type of pectin, functions in cell wall adhesion. Further, pectin methylesterase (PME) is involved in functionalization of homogalacturonan. We quantitated PME gene expression levels in differentiating xylem cells using different degrees of compression wood samples and examined the correlation with circularity of the transverse section of tracheids in each sample. We found that lower gene expression level of the sample corresponded with increasing circularity of the transverse section of tracheids. It is considered that the transverse section of compression wood tracheids becomes circular by suppression of PME gene expression during differentiation. Further, we observed the normal wood specific pectin methylesterase (CoPME) localization in differentiating xylem tracheids by immunolabeling. Labels localized at the entire perimeter of the compound middle lamella in normal wood, whereas sparse labeling was found in compression wood. It suggests that cell walls adhere at sites of CoPME function in differentiating xylem tracheids, but there is inadequate adhesion between cell walls where CoPME does not function. At the end of the expansion zone, the volume of the cell decreases due to a decrease in the turgor pressure of the tracheid. Further, due to moisture shrinkage of the tracheid, the adhesion begins to peel off in places of inadequate adhesion between cell walls, resulting in cell gaps and, thereby, generating a circular cell shape of cell wall formation in compression wood. 展开更多
关键词 REACTION WOOD WOOD Formation Cell Differentiation Immunohistochemistry Quantitative Polymerase Chain REACTION Monoclonal Antibody
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Clinical Significance of Serum Galectin-1 and Its Tissue Immunohistochemical Expression in Serous Ovarian Carcinoma Patients 预览
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作者 Mai Mohamed Abdelwahab Huda Fathy Ebian +5 位作者 Taiseer Refat Ibrahim Mohammad Samir Badr Mohamed El-Bakry Lashin Mahmoud Abdou Yassin Adel Mohamed Ismail Ahmed Ali Obaya 《妇产科期刊(英文)》 2019年第7期937-953,共17页
Objectives: Serous ovarian carcinoma (SOC) is the commonest ovarian carcinoma type with poor prognosis due to early metastasis and first presentation with advanced stage. In this work, we investigated serum level of G... Objectives: Serous ovarian carcinoma (SOC) is the commonest ovarian carcinoma type with poor prognosis due to early metastasis and first presentation with advanced stage. In this work, we investigated serum level of Galactin-1 (Gal-1) and its tissue immunohistochemical expression in SOC patients at different stages trying to find out its significance as a diagnostic and prognostic marker. Patients and methods: The study included 95 females I-Control group: Twenty five healthy females;II-Patients group: Seventy females diagnosed as SOC at different stages;Stage I: 8 cases, Stage II: 12 cases, Stage III: 32 cases and Stage VI:18 cases. Serum Galectin-1 and CA-125 were measured by ELIZA and tissue Galectin-1 was assessed by immunohistochemistry. All patients were followed for up to 3 years after surgery. Results: Serum Gal-1 and CA-125 levels were significantly higehr in SOC patients compared to controls (p 0.001). We found a direct positive statistically significant correlation between serum Gal-1 and CA125 levels (p 0.001). Serum Gal-1 at cut off value > 135 ng/ml was superior to CA-125 a cut off value > 49 u/ml with sensitivity, specificity of 100%, vs 88.57, 96% for CA-125. Serum Gal-1 was significantly associated with tumor stage (p 0.001). Immunohistochemistry showed that patients with strong Gal-1 expression had higher serum level (p = 0.002). Stromal and tumor Gal-1 expression were significantly correlated with tumor grade (p 0.001) and stage (p = 0.001). Serum Gal-1, CA-125 and IHC Gal-1 expression were associated with poor survival (p 0.001, p = 0.009 and p = 0.002) respectively. Conclusion: Serum Gal-1 and its tissue IHC expression are useful diagnostic and prognostic markers for SOC patients. 展开更多
关键词 SEROUS OVARIAN Carcinoma SERUM IMMUNOHISTOCHEMISTRY GALECTIN-1 Overall Survival
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A Case of Struma Ovarii Diagnosed by Cytology during Laparoscopic Surgery 预览
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作者 Kenji Niwa Motoki Takenaka +5 位作者 Tsuneo Ishihara Sakae Mori Keigo Kuwabara Saki Murase Yoshio Yamaguchi Takuji Tanaka 《病理学期刊(英文)》 2019年第1期1-9,共9页
We report a case of pure struma ovarii tumor diagnosed by cytology during laparoscopic surgery. The patient was a 34-year-old Japanese woman, gravida 1, para 1, who had the left adnexal mass, and was pre-operatively d... We report a case of pure struma ovarii tumor diagnosed by cytology during laparoscopic surgery. The patient was a 34-year-old Japanese woman, gravida 1, para 1, who had the left adnexal mass, and was pre-operatively diagnosed as left ovarian endometriotic cyst or mature cystic teratoma by magnetic resonance imaging findings. She underwent laparoscopy, and the content of the left ovarian cystic tumor was found to be yellow gelatinous material, suggesting mature cystic teratoma. The imprint cytology of the tumor showed benign glandular pattern, suggesting struma ovarii. Histopathological findings led us to the diagnosis of pure struma ovarii with positive reactions for thyroglobulin and thyroid transcription factor-1. No metastases or disseminated lesions were detected. The patient has no recurrent signs 7 months after the operation. 展开更多
关键词 Struma Ovarii CYTOLOGY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY THYROGLOBULIN THYROID TRANSCRIPTION Factor-1
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Neoneurogenesis in Squamous Cell Carcinoma of Tongue: A New Mechanism for Its Development 预览
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作者 Zhenqi Xu Jianwei Shang 《口腔学期刊(英文)》 2019年第5期102-107,共6页
Objectives: To explore the role of the growth of new nerves (neo-neurogenesis) in the tumorogenesis of squamous cell carcinoma of tongue. Materials and Methods: 10 formalin-fixed specimens were gained from patients di... Objectives: To explore the role of the growth of new nerves (neo-neurogenesis) in the tumorogenesis of squamous cell carcinoma of tongue. Materials and Methods: 10 formalin-fixed specimens were gained from patients diagnosed with tongue squamous cell carcinoma. Animal models were made by subcutaneous injection in the dorsal midline with Tca-8113 cell line. Mice were sacrificed 2, 4, 6 weeks after cell injection, and tumor tissues were fixed in 4% paraformaldehyde, embedded in paraffin, sectioned. Detection of neo-neurogenesis was stained by Neurofila-ment-L antibody (NF-L) using immunohistochemistry method (IHC) in biopsy from both human body and animal model. Results: IHC staining of NF-L is positive in all 10 paraffins of tongue squamous cell carcinoma sections which suggest that newly formed nerves are observed in tumor microenvironment. NF-L staining is also positive in the paraffins from animal models indicating that the tongue cancer recruits newly formed nerves in its tumorogenesis. Conclusions: Tumor neo-neurogenesis may play an important role in the pathogenesis and development of tongue cancer. From a therapeutic perspective, further studies on the topic may provide new clinical opportunity. 展开更多
关键词 Neoneurogenesis SQUAMOUS CELL CARCINOMA TONGUE Neurofilament-L IMMUNOHISTOCHEMISTRY
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CRKL and CK2 <i>β</i>over Expression Are Independent Predictors of Patients Survival in Endometrioid Endometrial Carcinoma 预览
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作者 Randa Mohamed Kaf Wafaa El-Beshbishi +6 位作者 Ahmed A. Obaya Rham Z. Ahmed Khaled Fathy Helal Mohamed SH. Ramadan Abdelwahab S. Almoregy Ibrahim A. Heggy Ola A. Harb 《妇产科期刊(英文)》 2019年第4期479-493,共15页
Context: The prognostic significance of expression of V-Crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) and Protein kinase CK2 β in endometroid carcinoma is not fully clarified. Aim: To assess the prognosti... Context: The prognostic significance of expression of V-Crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) and Protein kinase CK2 β in endometroid carcinoma is not fully clarified. Aim: To assess the prognostic values and clinicopathological roles of CRKL and CK2 β expression in endometroid carcinoma by correlation their expression levels with clinicopathological parameters, response to therapy, and recurrence of the tumor and patients survival. Settings: Faculty of Medicine, Zagazig University, and Mansoura University. Methods: CRKL and CK2 β expressions were evaluated in 50 paraffin blocks EC patients that were followed up for 5 years. The relationship between their level of expressions, clinicopathological criteria and prognosis of patients was analyzed. Statistical analysis used: SPSS 22.0 for windows (SPSS Inc., Chicago, IL, USA) and MedCalc windows (Software bvba 13, Ostend, Belgium). Results: High expression of CRKL and CK was positively correlated with higher grade of the tumor (p = 0.004 and 0.006 respectively), presence of L.N metastases (p = 0.009 and 0.003 respectively), presence of distant metastases (p = 0.029 and 0.003 respectively) and advanced FIGO stage (p 0.001), poor response to therapy (p = 0.046 and 0.005 respectively), higher incidence of recurrence of the tumor after therapy (p = 0.004 and 0.048 respectively), higher incidence of cancer progression p = 0.018 and 0.044 respectively), poor PFS (p = 0.008 and 0.013 respectively), and OS rates (p 展开更多
关键词 CRKL CK2 β Endometroid Carcinoma IMMUNOHISTOCHEMISTRY PROGNOSIS
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Clinic Pathological Study of an Eccrine Spiradenoma 预览
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作者 Xiao Lin Xiong Zhang 《生物科学与医学(英文)》 2019年第5期6-10,共5页
Eccrine Spiradenoma (ES) is an exceedingly rare sweat-gland tumor, it usually presents as a solitary lesion and painful nodule. ES is a kind of neoplasm with distinct histological characteristics and nonspecific clini... Eccrine Spiradenoma (ES) is an exceedingly rare sweat-gland tumor, it usually presents as a solitary lesion and painful nodule. ES is a kind of neoplasm with distinct histological characteristics and nonspecific clinical manifestations. Most ES cases have a benign course;however, malignant transformation would occur after a long period of latency. The diagnosis mostly depends on the clinic symptom, histological features and immunohistochemistry. Here, we report a case of ES and literature review. The aim of this study is to understand clinic and histological features for ES. 展开更多
关键词 ECCRINE spiradenoma CLINICAL PATHOLOGY IMMUNOHISTOCHEMISTRY DIAGNOSIS
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Primary Cutaneous Gamma Delta T Cell Lymphoma: A Clinicopathological Analysis 预览
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作者 Li Tang Yu Li 《生物科学与医学(英文)》 2019年第5期22-26,共5页
Primary cutaneous gamma-delta T-cell lymphoma (PCGD-TCL) is a very uncommon and extremely aggressive tumor, and is described in the newly re-vised World health organization for research and treatment of cancer classif... Primary cutaneous gamma-delta T-cell lymphoma (PCGD-TCL) is a very uncommon and extremely aggressive tumor, and is described in the newly re-vised World health organization for research and treatment of cancer classification of cutaneous lymphomas. A 43-year-old male patient presented with a 4 months history of cutaneous lesion over upper lip, without plaque and any constitutional symptom. Histopathological examination of skin biopsy revealed infiltration of atypical lymphocytes with hyperchromatic irregular nuclei. Immunophenotyping pattern of skin biopsy was compatible with PCGD-TLC. It is a highly aggressive tumor resistant to chemotherapy, immunotherapy, and radiation therapy. The GDTCL is characterized by a worse prognosis with a median survival of 15 months. Early diagnosis is essential and aggressive therapy is necessary. 展开更多
关键词 CUTANEOUS Gamma-Delta T-CELL LYMPHOMA HEMATOXYLIN and EOSIN IMMUNOHISTOCHEMISTRY
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Gastric Gist as Cause of High Digestive Hemorrhage 预览
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作者 Vinícius Moreira Paladino Thiago Sande Miguel +7 位作者 Vinicius Sande Miguel Marcia Machado Galv?o Pereira Fernando Antonio Galv?o Pereira Carolina Galv?o Teixeira Caroline Guida Babinski Nayrton Kalys Cruz dos Anjos Caio Marcus de Andrade Lopes Daniel Almeida da Costa 《生物科学与医学(英文)》 2019年第1期42-47,共6页
Introduction: Gastrointestinal stromal tumor (GIST) occurrence is uncommon and usually manifested by nonspecific signs and symptoms, which hinders its reasoning as a diagnostic hypothesis. The objective is to report a... Introduction: Gastrointestinal stromal tumor (GIST) occurrence is uncommon and usually manifested by nonspecific signs and symptoms, which hinders its reasoning as a diagnostic hypothesis. The objective is to report a case of female GIST with curative treatment through video-laparoscopy with partial gastrectomy. Case report: A 61-year-old female patient attended the Luiz Gioseffi Jannuzzi School Hospital (HELGJ) emergency room, with a sudden onset of upper gastrointestinal bleeding with hemodynamic instability. After stabilization, she was admitted for diagnostic investigation, showing sub-epithelial lesion of antrum in upper digestive endoscopy (UDE) and solid-cystic mass in the gastric antrum to computed tomography (CT). The conduct was the video laparoscopy surgical approach with partial gastrectomy. The histopathological and immunohistochemical aspects of the surgical specimen showed a gastrointestinal stromal tumor (GIST) in a gastric antrum of low grade of malignancy, positive e-Kit and mycotic index (50 cga) Discussion: Compared to the scarce reports in literature, the case described presented typical epidemiology, but the clinical picture was not the most incident. Conclusions: Given the rarity of this pathology cases, it is essential to report these in order to elucidate the specificities and also to discuss diagnostic and therapeutic methods. 展开更多
关键词 GASTROINTESTINAL STROMAL Tumor GASTROINTESTINAL HEMORRHAGE IMMUNOHISTOCHEMISTRY
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藤梨根提取物对宫颈癌的抑制作用
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作者 杨誉佳 陆远富 +2 位作者 安强 柏永华 张静静 《中华中医药学刊》 CAS 北大核心 2019年第7期1710-1714,I0033,I0034共7页
目的:通过一系列分子生物学方法研究藤梨根提取物对人宫颈癌Hela细胞的抑制,探索其发生抗肿瘤细胞作用可能的原理。方法:采用体外细胞培养方法,以不同浓度(1、10、100μg/mL)的藤梨提取物作用于HeLa细胞12、24 h,荧光显微镜观察细胞凋亡... 目的:通过一系列分子生物学方法研究藤梨根提取物对人宫颈癌Hela细胞的抑制,探索其发生抗肿瘤细胞作用可能的原理。方法:采用体外细胞培养方法,以不同浓度(1、10、100μg/mL)的藤梨提取物作用于HeLa细胞12、24 h,荧光显微镜观察细胞凋亡;荧光染色观察细胞核内变化;应用MTT法检测其对宫颈癌Hela细胞的抑制效果;流式术检测宫颈癌HeLa细胞的周期分布和凋亡率情况;琼脂糖凝胶电泳法观察HeLa细胞的DNA Aladder形成情况;免疫组化SP法观察Bcl-2、Bax、Caspase-3的表达情况;Real time-PCR法观察宫颈癌HeLa细胞内的COX-2和PCNA mRNA含量变化。结果:低、中、高剂量组作用于HeLa细胞12、24 h后,可见明显的细胞凋亡现象,且凋亡现象明显高于对照组,其中24 h藤梨根(100μg/mL)组凋亡率最高,达38.8%;细胞的增殖逐渐减少,细胞的生长周期主要集中在G0/G1期;DNA Aladder的合成受阻,高剂量组凋亡条带明显可见;较对照组相比,Bcl-2、Cox-2、Pcna基因表达明显减少,Bax及Caspase-3 mRNA表达显著增加,高剂量组(100μg/mL)尤为明显(均P<0.05)。结论:藤梨根提取物抑制宫颈癌HeLa细胞的生长效果良好,与HeLa细胞增殖减少和细胞凋亡的促进有关,而降低Bcl-2、Cox-2、Pcna基因表达、增强Bax基因表达,活化Caspase-3 mRNA也可能是其促进细胞凋亡机制之一。 展开更多
关键词 藤梨根 HELA细胞 细胞抑制 MTT法 流式细胞术 免疫组化 琼脂糖凝胶电泳
Rsf-1在胃癌和癌前病变组织中的表达及临床意义 预览
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作者 杨勒 薛立峰 +3 位作者 柳玉红 李晓鸣 吕志武 刘锦涛 《现代肿瘤医学》 CAS 2019年第8期1375-1380,共6页
目的:染色质重塑因子1(chromatin remodeling factor 1,Rsf-1)可作为肿瘤靶向治疗的靶点,因此探讨其在胃癌(gastric cancer)及癌前病变(precancerous lesions)组织中的表达及临床意义。方法:采用免疫组化SP法检测30例萎缩性胃炎、30例... 目的:染色质重塑因子1(chromatin remodeling factor 1,Rsf-1)可作为肿瘤靶向治疗的靶点,因此探讨其在胃癌(gastric cancer)及癌前病变(precancerous lesions)组织中的表达及临床意义。方法:采用免疫组化SP法检测30例萎缩性胃炎、30例肠上皮化生、22例不典型增生Rsf-1蛋白表达水平。并检测64例胃癌与配对癌旁组织(手术切缘距离肿瘤>5 cm)中Rsf-1和p53蛋白表达水平并分别分析与胃癌临床病理参数间的关系,同时分析Rsf-1与p53在胃癌中表达的相关性。结果:Rsf-1在胃癌、肠上皮化生、不典型增生组织中的阳性表达率均高于癌旁组织(P<0.05)。Rsf-1在胃癌中表达与淋巴结转移有关(P<0.05)。p53在胃癌中的阳性表达率高于癌旁组织,其表达与淋巴结转移有关(P<0.05)。p53和Rsf-1在胃癌中的表达存在正相关性(r=0.38,P<0.05),两者在胃癌中共阳性表达与淋巴结转移有关(P<0.05)。结论:Rsf-1可能参与了胃癌病变的发生及发展,对胃癌早期筛查具有重要指导意义。Rsf-1和p53两者共阳性表达对于预测胃癌淋巴结转移可能具有一定的参考价值。 展开更多
关键词 Rsf-1 P53 胃癌 癌前病变 免疫组织化学
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Brachyury在脊索瘤诊断中的应用
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作者 孙晓淇 丁宜 +3 位作者 张铭 宫丽华 王倩倩 王超 《诊断病理学杂志》 2019年第5期269-273,共5页
目的探讨Brachyury在脊索瘤中表达的敏感性和特异性,评价其应用价值。方法采用EnVision法检测Brachyury蛋白在40例骶骨脊索瘤中表达,另外选取40例组织形态与脊索瘤相类似的病例其中包括5例混合瘤(副脊索瘤/肌上皮瘤),8例骨外黏液样软骨... 目的探讨Brachyury在脊索瘤中表达的敏感性和特异性,评价其应用价值。方法采用EnVision法检测Brachyury蛋白在40例骶骨脊索瘤中表达,另外选取40例组织形态与脊索瘤相类似的病例其中包括5例混合瘤(副脊索瘤/肌上皮瘤),8例骨外黏液样软骨肉瘤,10例肾透明细胞癌和17例富含黏液的软骨肉瘤作为对照。同时比较CK,vimentin、EMA和S-100在上述肿瘤中表达情况。结果 40例脊索瘤病例中,Brachyury全部表达(40/40),表达强度为轻-重度,退分化脊索瘤中退分化成分不表达,对照组40例病例中2例混合瘤(副脊索瘤/肌上皮瘤)、2例骨外黏液样软骨肉瘤和2例富含黏液的软骨肉瘤表达Brachyury(6/40),表达强度为轻-中度,经统计学分析,Brachury在本组实验中表达敏感性为100%,特异性为85%。结论 Brachury是诊断脊索瘤敏感性和特异性均较好的免疫标记物,可以作为一线抗体使用,但也需要结合组织形态特点,标本处理情况及其他免疫组化抗体CK、EMA、vimentin、S-100等综合判断。 展开更多
关键词 BRACHYURY 脊索瘤 免疫组化
N-乙酰转移酶8在人肾透明细胞癌中的表达及临床意义 预览
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作者 张绍崎 唐朝朋 +2 位作者 沈天一 蔡维奇 周文泉 《中国现代医药杂志》 2019年第10期22-26,共5页
目的探讨N-乙酰转移酶8(N-acetyltransferase 8,NAT8)在人肾透明细胞癌(clear cell renal cell carcinoma,CCRCC)组织中的表达及临床意义,分析其表达与临床病理特征之间的关系。方法采用实时荧光定量PCR(qRTPCR)法检测12例CCRCC新鲜癌... 目的探讨N-乙酰转移酶8(N-acetyltransferase 8,NAT8)在人肾透明细胞癌(clear cell renal cell carcinoma,CCRCC)组织中的表达及临床意义,分析其表达与临床病理特征之间的关系。方法采用实时荧光定量PCR(qRTPCR)法检测12例CCRCC新鲜癌组织及其癌旁正常肾组织中NAT8的mRNA,分析其含量与CCRCC的关系;同时采用免疫组化法在蛋白水平验证CCRCC中NAT8的表达,分析其与CCRCC临床病理特征的关系。结果qRT-PCR显示CCRCC组织中NAT8mRNA的相对表达量明显低于癌旁正常肾组织,差异有统计学意义(P=0.003);免疫组织化学染色进一步显示NAT8蛋白在肾癌中呈低表达,癌旁正常肾组织中高表达,肾癌及癌旁组织中低表达率分别为60%(25/42)、12%(5/42),差异具有统计学意义(P<0.001),肿瘤直径≤7cm的患者中NAT8低表达15例(48.4%),肿瘤直径>7cm的患者中NAT8低表达10例(90.9%),差异有统计学意义(P=0.016);NAT8低表达在高分化、中分化、低分化肿瘤组织中分别为11例(44.0%)、14例(82.4%)、0例(0),差异有统计学意义(P=0.024);NAT8低表达在Ⅰ期、Ⅱ期肿瘤组织中分别为15例(48.4%)、10例(90.9%),差异有统计学意义(P=0.016);不同年龄、性别患者NAT8的表达差异无统计学意义(P=0.187、0.952)。结论NAT8在CCRCC肿瘤组织中呈低表达,其表达高低与肿瘤的大小、肿瘤分期及分级相关,提示其可能与CCRCC的发生、发展相关。 展开更多
关键词 N-乙酰转移酶8 肾透明细胞癌 实时荧光定量PCR 免疫组织化学
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RANK蛋白表达在胰腺癌组织中的临床意义 预览
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作者 肖烨 张健 《胃肠病学和肝病学杂志》 CAS 2019年第11期1277-1281,共5页
目的探讨核因子κB受体活化因子(receptor activator of nuclear factor-κB,RANK)蛋白表达在胰腺癌组织中的临床意义。方法收集2014年1月至2018年12月在徐州医科大学附属医院行胰腺癌切除手术的83例胰腺癌患者标本和临床资料,采用免疫... 目的探讨核因子κB受体活化因子(receptor activator of nuclear factor-κB,RANK)蛋白表达在胰腺癌组织中的临床意义。方法收集2014年1月至2018年12月在徐州医科大学附属医院行胰腺癌切除手术的83例胰腺癌患者标本和临床资料,采用免疫组织化学法检测RANK在胰腺癌组织及癌旁组织中的表达情况,并分析RANK与胰腺癌临床病理特征的关系,采用Kaplan-Meier法绘制生存曲线并分析RANK表达与不良预后的相关性。结果免疫组化结果显示,RANK在胰腺癌组织中的表达率显著高于癌旁组织(53.01%vs 10.84%),胰腺癌组织中RANK蛋白的表达IOD值显著高于癌旁组织[(5165.17±247.42)vs(1040.69±207.64)],差异有统计学意义(P<0.05)。RANK高表达与TNM分期、CA19-9水平及淋巴转移相关(P<0.05)。Spearman相关性分析结果显示,RANK表达与胰腺癌分化程度、TNM分期呈负相关(r=-0.479,P=0.014;r=-0.382,P=0.031),而RANK表达与CA19-9水平呈正相关(r=0.446,P=0.017)。生存分析结果显示,RANK高表达患者的5年生存率显著低于RANK低表达患者(P<0.05),RANK高表达与胰腺癌患者预后不良显著相关(P<0.05)。结论RANK蛋白在胰腺癌组织中高表达,且与恶性临床病理特征及不良预后相关,可作为胰腺癌早期诊断及预后判断的一项重要指标。 展开更多
关键词 胰腺癌 RANK 免疫组化 预后 临床意义
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新孢子虫急性感染对小鼠器官中TLR3表达的影响
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作者 苏钰 吕超超 +3 位作者 张慧芬 刘玉婷 纪伟霞 钱伟锋 《黑龙江畜牧兽医》 CAS 北大核心 2019年第14期55-59,173共6页
为了研究新孢子虫急性感染对小鼠体内不同器官中Toll样受体3(TLR3)表达的影响,试验将培养、纯化的新孢子虫Nc-1株速殖子通过腹腔注射途径感染5周龄C57BL/6小鼠(8×106个速殖子/只),感染后第2天和第8天分别采集小鼠的心脏、肝脏、脾... 为了研究新孢子虫急性感染对小鼠体内不同器官中Toll样受体3(TLR3)表达的影响,试验将培养、纯化的新孢子虫Nc-1株速殖子通过腹腔注射途径感染5周龄C57BL/6小鼠(8×106个速殖子/只),感染后第2天和第8天分别采集小鼠的心脏、肝脏、脾脏、肺脏和脑,通过荧光定量PCR和免疫组织化学方法,分别从mRNA和蛋白质水平检测各器官中TLR3的表达量。结果表明:感染组小鼠脾脏和肺脏中TLR3mRNA的相对表达量较对照组显著或极显著升高(P<0.05或P<0.01),其中脾脏中第2天和第8天TLR3mRNA的相对表达量为对照组的6倍或9倍,肺脏中TLR3mRNA的相对表达量约为对照组的4倍;肝脏和心脏中TLR3mRNA的相对表达量约为对照组的2倍,但差异均不显著(P>0.05),脑中TLR3mRNA的相对表达量未见明显变化。免疫组织化学结果与荧光定量PCR结果基本一致。说明新孢子虫急性感染可上调小鼠多个器官中TLR3的表达。 展开更多
关键词 新孢子虫 小鼠 TLR3 荧光定量PCR 免疫组化
食管神经内分泌癌107例的临床及病理特点分析
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作者 孟宇 练延帮 +4 位作者 李珊珊 勾贺 王亚梦 陈洁 宋敏 《中华消化杂志》 CAS CSCD 北大核心 2019年第8期533-538,共6页
目的探讨食管神经内分泌癌(ENEC)患者的临床表现及病理特点。方法纳入2011年1月至2018年11月郑州大学第一附属医院经病理复诊为ENEC的107例患者,分析其临床表现、肿瘤的部位、肿瘤大小、临床病理分型和免疫组织化学标志物情况。计量资... 目的探讨食管神经内分泌癌(ENEC)患者的临床表现及病理特点。方法纳入2011年1月至2018年11月郑州大学第一附属医院经病理复诊为ENEC的107例患者,分析其临床表现、肿瘤的部位、肿瘤大小、临床病理分型和免疫组织化学标志物情况。计量资料采用统计描述,分类资料采用卡方检验。结果107例ENEC患者中,首发症状以进食梗噎最常见,占63.6%(68/107);其次为胸背疼痛,占13.1%(14/107)。60.7%(65/107)的患者经内镜活组织检查确诊,39.3%(42/107)的患者经手术病理确诊。肿瘤位于食管胸上段相对较少,占13.1%(14/107);位于胸中段和胸下段分别占45.8%(49/107)和41.1%(44/107)。肿瘤长径为0.7~9.0 cm,中位数为2.5 cm,其中≤2.5 cm者占57.0%(61/107),>2.5 cm者占43.0%(46/107)。107例患者中,溃疡型50例(46.7%),髓质型32例(29.9%),蕈伞型16例(15.0%),隆起型9例(8.4%)。107例患者中,纯神经内分泌癌(P-NEC) 96例(其中小细胞型95例,大细胞型1例),占89.7%;混合性神经内分泌癌(M-NEC)11例(其中小细胞癌鳞状细胞癌混合型9例,小细胞癌腺癌混合型2例),占10.3%。突触素阳性率为99.0%(104/105),CD56阳性率为98.0%(100/102),嗜铬粒蛋白A(CgA)阳性率为31.5%(17/54),Ki-67为>90%~100%占47.7%(51/107)。肿瘤最大径>2.5 cm的P-NEC占42.1(45/107),M-NEC占0.9%(1/107),P-NEC组的肿瘤最大径大于M-NEC组,差异有统计学意义(χ^2=4.311,P=0.038)。结论ENEC是一种临床表现不特异且有高度侵袭性的恶性肿瘤,确诊主要靠组织病理及免疫组织化学检查。 展开更多
关键词 神经内分泌 食管 免疫组织化学 病理学 临床
蕈样肉芽肿患者临床资料32例回顾性研究
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作者 耿祎喆 李冬芹 《医药论坛杂志》 2019年第7期69-72,共4页
目的对32例蕈样肉芽肿患者的临床资料进行全面分析,提高临床大夫对本病的认识,降低临床误诊率,旨在对本病达到早期诊断,及早治疗,改善愈后的目的。方法回顾性分析2012年—2019年在郑州大学第一附属医院皮肤科住院治疗的、经临床及组织... 目的对32例蕈样肉芽肿患者的临床资料进行全面分析,提高临床大夫对本病的认识,降低临床误诊率,旨在对本病达到早期诊断,及早治疗,改善愈后的目的。方法回顾性分析2012年—2019年在郑州大学第一附属医院皮肤科住院治疗的、经临床及组织病理确诊的、具有完整病理资料的32例蕈样肉芽肿患者,分析其社会人口统计学数据、临床症状和体征、疾病分期分类、实验室检查结果数据、误诊情况。结果 32例MF患者中,男性23例,女性9例,男:女比例2.55∶1,平均发病年龄(45.13±17.47)岁。其中红斑期15例(47%),斑块期13例(40%),肿瘤期4例(13%)。出现亲表皮现象17例(51%),典型Pautrier微脓肿14例(43%)。32例中免疫组化结果提示有32例CD3~+(100%),29例CD4~+(90%),22例CD8~-(69%),26例CD20~-(81%),12例患者进行了基因重排检测,10例(83%)检测出克隆性T细胞受体(TCR)基因重排。32例蕈样肉芽肿患者中,有19例(58%)患者被误诊为其他疾病。结论①蕈样肉芽肿皮肤表现多样性,临床容易误诊。②蕈样肉芽肿早期组织病理不典型,临床容易漏诊。 展开更多
关键词 皮肤T淋巴细胞瘤 蕈样肉芽肿 临床病理 免疫组化 误诊
胃癌中PD-L1蛋白表达及分布特征的病理学意义 预览
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作者 王岳君 王娜娜 +4 位作者 杨苗苗 王源 孙晶璐 章俊生 孟刚 《临床与实验病理学杂志》 CAS CSCD 北大核心 2019年第9期1053-1059,共7页
目的检测胃癌中程序性死亡配体-1(programmed death ligand-1,PD-L1)蛋白的表达,总结其在癌组织及癌间质中肿瘤浸润单核细胞(tumor infiltrating mononuclear cells,TIMCs)中的表达水平及分布特征,探讨影响胃癌PD-L1蛋白表达的病理学因... 目的检测胃癌中程序性死亡配体-1(programmed death ligand-1,PD-L1)蛋白的表达,总结其在癌组织及癌间质中肿瘤浸润单核细胞(tumor infiltrating mononuclear cells,TIMCs)中的表达水平及分布特征,探讨影响胃癌PD-L1蛋白表达的病理学因素,以提高其判读的准确性及可重复性。方法收集安徽医科大学第四附属医院2015~2019年期间110例行胃癌根治切除术标本,运用免疫组化MaxVision两步法检测PD-L1蛋白表达,观察其在癌组织及TIMCs中的表达水平及分布特征,计算并比较不同判读方法下PD-L1蛋白表达及其检测阳性率的差异,并分析其与临床病理学特征及临床预后的关系。结果运用CPS、TPS及MIDS三种不同的判读方法,胃癌中PD-L1蛋白的检测阳性率分别为46.4%、24.5%、42.7%,三者一致性较好(P<0.05)。其中运用CPS检测的阳性率最高,且呈高表达者最多。CPS、TPS及MIDS三者检测阳性率与胃癌肿瘤实质性坏死、TIMCs数量、肿块大小呈正相关(P<0.05),其中CPS检测PD-L1表达还与癌组织浸润深度相关(P<0.05),但与患者性别、年龄、肿瘤病变部位、组织学类型等均无关。PD-L1蛋白高表达者在胃癌组织的分布以弥漫性分布特征为主,低表达者以斑驳样分布特征为主,且无论表达高低均存在癌巢与间质接触面区域PD-L1着色强度较高的苔藓样分布特征。TIMCs中PD-L1阳性表达亦以苔藓样分布特征为主,着色细胞主要是淋巴细胞、巨噬细胞等,且接触面并列分布的癌细胞也更易见PD-L1阳性表达。结论胃癌中PD-L1蛋白检测以CPS判读指标为优。进展期胃癌中,当肿块直径>5.0 cm、TIMCs数目较多、肿瘤实质性坏死明显时,PD-L1均易呈阳性,且癌巢与间质接触面区域易出现PD-L1表达或表达较高的情况,判读时要注意观察。 展开更多
关键词 胃肿瘤 PD-L1 免疫组化 结果判读
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Follicular dendritic cell sarcoma detected in hepatogastric ligament: A case report and review of the literature 预览
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作者 Wen-Xin Yan You-Xi Yu +2 位作者 Ping Zhang Xing-Kai Liu Yan Li 《世界临床病例杂志》 2019年第1期116-121,共6页
The most common organ where follicular dendritic cell sarcoma (FDCS) occurs is in cervical lymph nodes, while few cases are found in extranodal organs such as liver, spleen, and soft tissue. This is a case report that... The most common organ where follicular dendritic cell sarcoma (FDCS) occurs is in cervical lymph nodes, while few cases are found in extranodal organs such as liver, spleen, and soft tissue. This is a case report that FDCS occurs in the hepatogastric ligament. To our knowledge, there is no such case that has been reported previously. A 47-year-old male patient was found to have an intraabdominal mass during an annual physical examination. Computed tomography showed a 4.2 cm × 4.1 cm mass located at the lesser curvature of the stomach, above the pancreas. During operation, a tumor mass was found in the hepatogastric ligament and a radical resection was performed. The tumor was diagnosed as FDCS by pathology and immunohistochemical testing. The patient had a favorable recovery, and no obvious abnormality was found 3 months postoperation. 展开更多
关键词 FOLLICULAR DENDRITIC cell SARCOMA Hepatogastric LIGAMENT Pathology IMMUNOHISTOCHEMISTRY Computed tomography Case report
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结肠癌组织RASGRF1蛋白表达与基因甲基化状态变化及其临床意义 预览
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作者 钱伟明 王海燕 李可 《山东医药》 CAS 2019年第19期10-13,共4页
目的探讨结肠癌组织Ras蛋白特异性鸟苷酸释放因子1(RASGRF1)蛋白表达与基因甲基化状态变化及其临床意义。方法取手术切除的结肠癌组织及其配对的癌旁正常组织各81例份,采用免疫组化法检测RASGRF1蛋白表达,采用甲基化特异性PCR法检测RASG... 目的探讨结肠癌组织Ras蛋白特异性鸟苷酸释放因子1(RASGRF1)蛋白表达与基因甲基化状态变化及其临床意义。方法取手术切除的结肠癌组织及其配对的癌旁正常组织各81例份,采用免疫组化法检测RASGRF1蛋白表达,采用甲基化特异性PCR法检测RASGRF1基因甲基化状态。分析RASGRF1蛋白表达与基因甲基化状态以及二者与患者临床病理特征的关系。结果结肠癌组织RASGRF1蛋白阳性表达率明显低于癌旁正常组织(χ^2=51.995,P<0.01),RASGRF1基因甲基化阳性率明显高于癌旁正常组织(χ^2=44.100,P<0.01)。结肠癌组织RASGRF1蛋白阳性表达与RASGRF1基因甲基化阳性呈负相关关系(ρ=-0.486,P<0.05)。结肠癌组织RASGRF1蛋白阳性表达与肿瘤TNM分期、淋巴结转移有关(P均<0.05),与患者性别、年龄、肿瘤直径、组织分化程度无关(P均>0.05);结肠癌组织RASGRF1基因甲基化阳性与肿瘤TNM分期、淋巴结转移有关(P均<0.05),与患者性别、年龄、肿瘤直径、组织分化程度无关(P均>0.05)。结论结肠癌组织RASGRF1蛋白低表达、RASGRF1基因甲基化异常升高,二者呈负相关关系,且均与肿瘤TNM分期和淋巴结转移有关。 展开更多
关键词 结肠癌 Ras蛋白特异性鸟苷酸释放因子1 基因甲基化 临床病理特征 免疫组化 甲基化特异性PCR
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Notch3在肾透明细胞癌中的表达及临床意义 预览
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作者 韩冬冰 寇宏博 +1 位作者 范以生 韩起鹏 《现代肿瘤医学》 CAS 2019年第16期2891-2894,共4页
目的:通过研究Notch3蛋白在肾透明细胞癌组织和癌旁组织中的表达变化情况,探讨其临床意义及相关影响因素。方法:采用免疫组织化学染色检测57例肾透明细胞癌组织、38例癌组织外缘1.0cm处组织中的Notch3蛋白表达情况,并观察其与临床病理... 目的:通过研究Notch3蛋白在肾透明细胞癌组织和癌旁组织中的表达变化情况,探讨其临床意义及相关影响因素。方法:采用免疫组织化学染色检测57例肾透明细胞癌组织、38例癌组织外缘1.0cm处组织中的Notch3蛋白表达情况,并观察其与临床病理参数之间的关系。结果:肾透明细胞癌、癌旁组织中Notch3蛋白的阳性表达率分别为80.7%(46/57)、31.6%(12/38),P<0.05。单因素分析表明,Notch3蛋白的表达与肾透明细胞癌的临床分期、病理学分级、远处转移密切相关(P<0.05)。多因素分析表明,患者的年龄、临床分期、吸烟史是Notch3蛋白在肾透明细胞癌中表达的独立危险因素(P<0.05)。结论:Notch3蛋白在肾透明细胞癌组织中的表达明显增高,Notch3可能成为肾透明细胞癌的潜在基因治疗靶点和早期诊断检测及预防的指标之一。 展开更多
关键词 NOTCH3 肾透明细胞癌 免疫组织化学
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