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APE1基因多态性与南通地区汉族肝癌患者TACE疗效及预后的关联研究
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作者 陆小华 朱小庆 +2 位作者 袁洪新 张玉宇 储玉山 《生物医学工程与临床》 CAS 2019年第2期205-211,共7页
目的探讨脱嘌呤脱嘧啶核酸内切酶(APE1)基因141位点T/G、148位点T/G多态性与南通地区肝癌患者经动脉插管化学治疗栓塞术(TACE)疗效及预后的相关性。方法选择2012年1月1日至2013年12月31日在南通大学附属医院介入放射科住院的239例汉族... 目的探讨脱嘌呤脱嘧啶核酸内切酶(APE1)基因141位点T/G、148位点T/G多态性与南通地区肝癌患者经动脉插管化学治疗栓塞术(TACE)疗效及预后的相关性。方法选择2012年1月1日至2013年12月31日在南通大学附属医院介入放射科住院的239例汉族肝癌患者,其中男性163例,女性76例;年龄24~83岁,平均年龄55.61岁。吸烟史84例,无155例;有饮酒史103例,无136例;乙型肝炎病毒感染205例,无34例;巴塞罗那临床肝癌(BCLC)分期B期194例,C期45例;甲胎蛋白(AFP)> 400 ng/mL 186例,≤400 ng/mL 53例;Child-Pugh分级A级221例,B级18例。所有患者均行TACE治疗,在治疗前采集外周静脉血。利用两对引物-聚合酶链反应(PCR-CTPP)技术进行APE1基因141、148位点基因分型。治疗后1个月均复查腹部增强CT、AFP等。随访患者TACE治疗3年生存情况。结果 TACE治疗次数≥4次137例,<4次102例。TACE临床疗效评价完全缓解11例,部分缓解104例,稳定87例,控制率为84.52%;3年随访86例生存患者,生存率为35.98%。携带APE1基因141位点T/T基因型143例,携带APE1基因141位点T/G+G/G基因型96例;携带APE1基因141位点T/T基因型患者TACE治疗有效率88.81%,携带T/G+G/G基因型有效率78.13%。携带APE1基因148位点T/T基因型128例,携带APE1基因148位点T/G+G/G基因型111例;携带APE1基因148位点T/T基因型患者TACE治疗有效率86.72%,携带T/G+G/G基因型有效率81.98%。携带APE1基因141位点T/T基因型患者TACE治疗有效率高于T/G+G/G基因型,差异有统计学意义(P <0.05)。Logistic回归分析,患者年龄、性别、吸烟史、饮酒史、有无乙型肝炎病毒感染、BCLC分期、AFP水平、Child-Pugh分级及TACE治疗次数与疗效无关,校正后仅APE1基因141位点基因型与治疗疗效有关;APE1基因148位点T/T基因型与T/G+G/G基因型患者之间TACE治疗有效率比较,差异无统计学意义(P> 0.05)。携带APE1基因141位点T/T基因型患者3年生存率41.26%,携带T/G+G/G基因型� 展开更多
关键词 脱嘌呤脱嘧啶核酸内切酶(APE1)基因 基因多态性 肝癌 经动脉插管化学治疗栓塞术(TACE) T/T基因型 T/G+G/G基因型
Heterogeneity of <i>Pestivirus</i>Species in Asia 预览
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作者 Massimo Giangaspero Shu-Qin Zhang Claudio Apicella 《微生物学(英文)》 2019年第3期266-342,共77页
Pestivirus are responsible for cosmopolitan diseases affecting cattle, pigs and other ruminants, presenting a wide range of clinical manifestations, with relevant impact on zootechnic production. Understanding genomic... Pestivirus are responsible for cosmopolitan diseases affecting cattle, pigs and other ruminants, presenting a wide range of clinical manifestations, with relevant impact on zootechnic production. Understanding genomic characteristic and virus taxonomy is fundamental in order to sustain control and prophylactic programs. Given the recent various studies reporting a relatively high number of new strains, in particular from Asian countries, in the present study, six hundred-fifty-one genomic sequences have been considered applying the palindromic nucleotide substitutions method for genotyping. Based on the secondary structure analysis of the 5’ untranslated region of RNA, sequence characteristics among Asian genomic clusters within the different Pestivirus species suggested geographic segregation and occurrence of micro-evolutive steps in the genus evolutionary history. This aspect was particularly evident in atypical sequences originated from China or Turkey, indicating risk of diffusion by animals and products trade or contamination of biological products as bovine calf serum, with potential diagnostic and control difficulties. 展开更多
关键词 ASIA GENOTYPES PESTIVIRUS Secondary Structure
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Mango Yield Performance in Lake Victoria Cresent Region of Uganda 预览
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作者 G. Ddamulira I. Ramathani +4 位作者 T. Sebikejje R. Naluyimba A. Otim A. Pariyo M. Maphosa 《美国植物学期刊(英文)》 2019年第7期1141-1153,共13页
A study was conducted to evaluate yield performance of mango (Mangifera indica L.) genotypes in the Lake Victoria Crescent zone. The experiment was superimposed on a seven-year-old mango field with 36 genotypes laid o... A study was conducted to evaluate yield performance of mango (Mangifera indica L.) genotypes in the Lake Victoria Crescent zone. The experiment was superimposed on a seven-year-old mango field with 36 genotypes laid out in a randomized complete block design, replicated thrice. Mango fruit set, fruit drop and yield were significantly (P 0.05) differently among years and genotypes. The highest fruit set was recorded in 2015 and the lowest in 2014. Among genotypes, the maximum and minimum fruit set were observed in Koona and Pinero, respectively. The highest fruit drop was observed in Heidi followed by Keitt and MP1 genotypes. The highest yield of 59.6 kg/tree/year was recorded in Kate while Kensington pride genotype yielded least (3.5 kg/tree/year). In this study variation in mango yield was mainly due to varietal differences. The findings will aid in selecting mango genotype suitable for production in Lake Victoria Crescent agro-ecological zone. 展开更多
关键词 GENOTYPES FRUIT SET MANGIFERA INDICA
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Genotypic Analysis Revealed Association of HLA Alleles with Clinical Parameters in Bangladeshi Patients with Rheumatoid Arthritis 预览
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作者 Fauzun Shaona Rubyyat Hassan +3 位作者 Sajib Chakraborty Shahanaz Sultana Jobaida Akther AHM Nurun Nabi 《美国分子生物学期刊(英文)》 2019年第4期173-190,共18页
This study investigated distribution of HLA alleles (HLADRB1*01, *03, *04, *07, HLA-DQB1*0201, *0301/4) in 34 healthy controls and 57 rheumatoid arthritis (RA) patients in a Bangladeshi population and correlated the g... This study investigated distribution of HLA alleles (HLADRB1*01, *03, *04, *07, HLA-DQB1*0201, *0301/4) in 34 healthy controls and 57 rheumatoid arthritis (RA) patients in a Bangladeshi population and correlated the genotypic frequencies with clinical parameters. Frequency distribution of HLA-DRB1*04 (34%) and HLA-DRB1*01 (32%) were the highest followed by HLA-DQB1*0301/4 (29%) and HLA-DQB1*0201 (26%) in RA patients while HLA-DRB1*03 (12%) had lowest frequency. Plasma level of anti-CCP and rheumatoid factor antibodies confirmed diagnosis of RA patients that varied significantly between patients and healthy controls. Likewise, plasma levels of C-reactive protein, triglycerides, cholesterol, HDL-cholesterol and activities of AST and ALT exhibited significant variation between the two groups. In contrast, the levels of glucose, total protein, uric acid, LDL-cholesterol and plasma activity of ALP in RA patients had no significant deviations from healthy controls. Relationship between HLA genotype frequency and clinical parameters revealed that the mean levels of anti-CCP and rheumatoid factor antibodies were highest in the patients harboring HLA-DRB1*04 allele. These findings underpin the correlation between HLA genotype with clinical markers of RA which are indicative of disease severity. The positive correlation of these markers with certain HLA genes may be used to identify susceptible individuals who are likely to have RA in Bangladeshi population. 展开更多
关键词 RHEUMATOID ARTHRITIS HLA ALLELE BANGLADESHI Population Gene Polymorphisms GENOTYPES
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Molecular Epidemiology of High-Risk Human Papilloma Virus Infection in Sexually Active Women at Bobo-Dioulasso University Teaching Hospital 预览
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作者 Souleymane Ouattara Der Adolphe Somé +6 位作者 Adama Dembélé Salif Sanfo Théodorat Zohoncon Abdoul-Karim Ouattara Moussa Bambara Blami Dao Jacque Simporé 《妇产科期刊(英文)》 2019年第8期1178-1188,共11页
The objective of this study was to determine the prevalence of HPV-HR genotypes in the population of sexually active women in Bobo-Dioulasso, Burkina Faso. Methods: This study took place at Souro Sanou Teaching Hospit... The objective of this study was to determine the prevalence of HPV-HR genotypes in the population of sexually active women in Bobo-Dioulasso, Burkina Faso. Methods: This study took place at Souro Sanou Teaching Hospital in Bobo-Dioulasso from September to June 2017. A total of 234 women in the gynecological period and also sexually active were enrolled after they gave an individual consent. Swabbing of the endocervical canal was done. From the sample stored at -20°C, the viral DNA was extracted using the “DNA-Sorb-A” kit from SACACE biotechnologies&reg. Amplification of the PCR of the extracted DNA was made, using the “HPV Genotypes 14 Real-TM Quant” V67-100 FRT kit. Data were analyzed with SPSS software version 17.0 and Epi Info 6. Chi-square and Fisher’s tests were used to compare proportions and averages;a link was significant when p 0.05. Results: The mean age was 30.7 ± 7.3 years (median: 30 years);84.5% of them were married, 43.5% had a socio-professional activity and 61% were schooled. A total of 20.6% of women were positive for at least one of the following HPV-HR genotypes: HPV 18, 31, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68. Genotypes the most frequently found were HPV 52 with 11 cases (17.2%) and 66 with 10 cases (15.6%). Conclusion: Our results show a predominance of HPV-HR genotypes not covered by available vaccines. Mapping genotypes are needed to fully understand viral ecology at the national level. But for instance, the nonavalent vaccine, which has a better coverage of the predominant genotypes, is to be promoted. 展开更多
关键词 HPV-HR EPIDEMIOLOGY GENOTYPES Bobo-Dioulasso
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北京地区妇科门诊患者沙眼衣原体和人类乳头瘤病毒感染及其基因型相关分析 预览
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作者 洪萍 王培昌 +1 位作者 刘宇 兰贺 《国际检验医学杂志》 CAS 2019年第6期649-652,共4页
目的分析北京地区妇科门诊因生殖道感染就诊的患者沙眼衣原体(CT)和人类乳头瘤病毒(HPV)感染及HPV基因型的分布情况。方法选取2016年12月至2017年12月于首都医科大学宣武医院妇科门诊患者的宫颈细胞标本1 768例,分别采用实时荧光聚合酶... 目的分析北京地区妇科门诊因生殖道感染就诊的患者沙眼衣原体(CT)和人类乳头瘤病毒(HPV)感染及HPV基因型的分布情况。方法选取2016年12月至2017年12月于首都医科大学宣武医院妇科门诊患者的宫颈细胞标本1 768例,分别采用实时荧光聚合酶链反应(PCR)法和PCR导流杂交法对CT、HPV病原体进行检测。结果在1 768例患者中,CT阳性率为11.1%(196/1 768),HPV阳性率为29.6%(524/1 768)。HPV感染单一亚型者414例(79.0%),多重亚型者110例(21.0%)。524例HPV阳性样本中,有122例CT检测阳性(23.3%),其中单一亚型者48例(39.3%),多重亚型者74例(60.7%),多重亚型患者阳性率明显高于单一HPV感染患者,差异有统计学意义(P<0.05)。在HPV合并CT感染患者中,多重亚型感染最常见的HR-HPV 16型的阳性率为13.0%(35/270),其次为52型11.1%(30/270),58型35.1%(26/270),33型8.1%(22/270)。HPV合并CT感染患者多重亚型的阳性率均高于单一HPV感染患者,差异有统计学意义(P<0.05)。结论对CT和HPV感染及HPV基因型的相关分析,进一步证明CT感染对于HPV的感染具有促进作用,预防和积极治疗CT感染对HPV感染的防治应具有积极意义,进而降低HPV感染诱发宫颈癌的可能性。 展开更多
关键词 沙眼衣原体 人类乳头瘤病毒 基因型 多重亚型 宫颈癌
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贵州省铜仁地区珠蛋白生成障碍性贫血患者基因分析及分布特征 预览
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作者 田敏 李丽 +2 位作者 毛万成 田晓静 杨韬 《现代检验医学杂志》 CAS 2019年第3期51-54,共4页
目的了解贵州省铜仁地区珠蛋白生成障碍性贫血基因分布情况及种族差异。方法选取2018年6月~2019年1月来医院就诊的400例疑似珠蛋白生成障碍性贫血样本,采用PCR+导流杂交法进行基因确诊,并对不同民族的基因型分布进行分析。结果在400例... 目的了解贵州省铜仁地区珠蛋白生成障碍性贫血基因分布情况及种族差异。方法选取2018年6月~2019年1月来医院就诊的400例疑似珠蛋白生成障碍性贫血样本,采用PCR+导流杂交法进行基因确诊,并对不同民族的基因型分布进行分析。结果在400例疑似标本中,188例确诊为珠蛋白生成障碍性贫血,占47%,其中α-珠蛋白生成障碍性贫血84例(21%),β-珠蛋白生成障碍性贫血100例(25%)。α-珠蛋白生成障碍性贫血基因均以缺失型为主,并以-SEA/αα(54.7%)和-α3.7/αα(38.2%)最为常见。β-珠蛋白生成障碍性贫血中,以基因型βCD17/βN(44%)和βCD41-42/βN(36%)为主。进一步对汉族、土家族、苗族及其他民族中不同类型珠蛋白生成障碍性贫血检出率进行比较,发现β-珠蛋白生成障碍性贫血在各民族间检出率差异有统计学意义(χ^2=8.5,P<0.05)。且该类型携带者在汉族人群中以βCD17/βN基因型居多(54.9%),在土家族与苗族人群中均以βCD41-42/βN基因型居多(53.8%和33.3%)。结论贵州省铜仁地区珠蛋白生成障碍性贫血携带者以-SEA/αα,βCD17/βN突变类型居多;β-珠蛋白生成障碍性贫血分布在铜仁地区具有种族差异性。 展开更多
关键词 珠蛋白生成障碍性贫血 基因型 分布特征
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徐州地区73株大肠杆菌O157:H7的遗传多样性分析 预览
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作者 顾玲 祖荣强 +2 位作者 周璐 杨华富 张雪峰 《江苏预防医学》 CAS 2019年第1期30-32,共3页
目的 了解不同来源大肠杆菌O157:H7菌株遗传标志物的分布频率。方法对1999年徐州地区O157:H7暴发疫情中分离到的73株O157:H7菌株,用PCR方法对stx1和stx2阳性菌株分别进行亚型分型;对stx2阳性菌株检测q933和q21基因;用RT-PCR进行tir等位... 目的 了解不同来源大肠杆菌O157:H7菌株遗传标志物的分布频率。方法对1999年徐州地区O157:H7暴发疫情中分离到的73株O157:H7菌株,用PCR方法对stx1和stx2阳性菌株分别进行亚型分型;对stx2阳性菌株检测q933和q21基因;用RT-PCR进行tir等位基因检测。结果73株菌中除1株tir(255A)和tir(255T)均为阴性外,其余菌株均携带tir(255T)基因;10株携带stx1,亚型分型检测显示均为stx1a;全部分离株均为stx2阳性,其中携带stx2a13株(17.81%),携带stx2c60株(82.19%)。食品中未检出stx2a;病人分离株中stx2a检出率高于动物分离株,差异有统计学意义(P<0.01),食品、动物分离株中stx2c检出率(100.00%、87.30%)较高。13株(17.81%)菌株为单q933阳性,60株(82.19%)为单q21阳性。食品中未检出q933,病人分离株中检出率高于动物分离株(P<0.01);q21在病人分离株中未检出,食品、动物分离株检出率较高(100.00%、87.30%)。结论不同来源的O157:H7分离菌株的遗传标志存在多样性,这可能有助于从外环境来源菌株中识别具有潜在人类疾病风险的基因型。 展开更多
关键词 大肠杆菌O157:H7 志贺毒素 基因亚型 遗传标志物
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热带西太平洋浮游有孔虫Globigerinoides ruber(白色)的基因型研究及其古海洋学意义
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作者 李青霞 类彦立 +2 位作者 李铁刚 翦知湣 石峻峰 《微体古生物学报》 CSCD 北大核心 2019年第1期1-11,共11页
Globigerinoides ruber是最常见的热带-亚热带浮游有孔虫种类,被广泛地应用于古环境重建。本实验通过浮游生物垂直拖网采集热带西太平洋海区目标站位表层200 m以浅水体中的浮游有孔虫,在体视显微镜下挑选出单个活体G.ruber(白色)个体,采... Globigerinoides ruber是最常见的热带-亚热带浮游有孔虫种类,被广泛地应用于古环境重建。本实验通过浮游生物垂直拖网采集热带西太平洋海区目标站位表层200 m以浅水体中的浮游有孔虫,在体视显微镜下挑选出单个活体G.ruber(白色)个体,采用DOC法提取虫体的总DNA,利用有孔虫特异性引物扩增SSU rDNA目的片段。我们通过构建系统发育树研究了G.ruber(白色)基因型多样性,并比较了不同基因型G.ruber(白色)的形态差异和分布特点。ML/BI树显示:在热带西太平洋海区获得的G.ruber(白色)样本中,有两大基因型Type I和Type II,Type I又包括Type Ia、Type Ib2两种类型。Type I和Type II个体的形态特征和栖息环境存在明显差异:Type I个体的三个房室呈显著的球状,主要分布在表层30 m以浅的水体中;Type II个体的三个房室呈扁平的近似球状,主要分布在水深30 m以下的水体中。本工作是对热带西太平洋浮游有孔虫DNA分子数据的重要补充,旨在为古海洋学重建提供更加精确的替代性指标。 展开更多
关键词 浮游有孔虫 Globigerinoides ruber SSU rDNA 基因型 热带西太平洋 古海洋学
深圳市罗湖区人乳头瘤病毒感染基因型别及分布情况分析
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作者 许晓清 刘惠玲 +3 位作者 张水兰 阚丽娟 刘小柳 莫红梅 《国际免疫学杂志》 CAS 2019年第3期257-262,共6页
目的探讨深圳市罗湖区15种人乳头瘤病毒(human papilloma virus,HPV)基因型的分子流行病学特点。方法选取2017年1月至2017年12月在深圳罗湖区人民医院妇科、体检科及社康中心接受HPV检测的22 496例女性患者作为研究对象,依据样本来源分... 目的探讨深圳市罗湖区15种人乳头瘤病毒(human papilloma virus,HPV)基因型的分子流行病学特点。方法选取2017年1月至2017年12月在深圳罗湖区人民医院妇科、体检科及社康中心接受HPV检测的22 496例女性患者作为研究对象,依据样本来源分为妇科组、体检组与社康组;按年龄分为5个年龄段:≤25岁,26~35岁,36~45岁,46~55岁,≥56岁。采用荧光PCR法检测15种高危型HPV(16、18、31、33、35、39、45、51、52、53、56、58、59、66、68型)感染情况。结果总体感染率为15.30%(3443/22 496),妇科组患者感染率为16.58%(2614/15 767),社康组感染率为14.03%(429/3058),体检组感染率为10.90%(400/3671)。高危型HPV感染年龄呈"U"形分布,≤25岁年龄段感染率最高,其次为≥56岁年龄段。总体感染率排名前五位的HPV基因型别依次为52、58、16、39、51型。各年龄段以单一感染最多见,多重感染以二重感染为主。结论深圳市罗湖区HPV感染亚型分布存在地区特异性,五个年龄段及三组不同风险人群HPV感染状况亦有差异。 展开更多
关键词 人乳头瘤病毒 基因分型 感染率
Hepatitis C Virus Genotyping in Saudi Population 预览
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作者 Ali Hazazi Mohammed Almubarak +3 位作者 Fawaz Albloui Omer Alsaweed Yousef Hawsawi Faisal Alseraye 《美中医学:英文版》 2019年第3期144-148,共5页
Background:Hepatitis C is one of the common types of viral hepatitis that threatens human life worldwide.Hepatitis C virus(HCV)is a single-strand RNA virus that has the ability to be transmitted by contaminated blood ... Background:Hepatitis C is one of the common types of viral hepatitis that threatens human life worldwide.Hepatitis C virus(HCV)is a single-strand RNA virus that has the ability to be transmitted by contaminated blood products and intravenous drug use.HCV is characterized by its ability to have a high genetic diversity,which eventually results in different HCV genotypes and numerous subtypes;however,this genetic variety is a core reason for the lack of a successful vaccine for these types of viruses.Methods:HCV nucleic acid was isolated from 597 patients diagnosed with chronic hepatitis C in Security Forces Hospital(SFHR)in Riyadh.HCV nucleic acid was extracted to make the target accessible for amplification and genotype detection.The process of nucleic acid extraction and amplification was accomplished with the Abbott Molecular Systems m2000sp and m2000rt using the Abbott RealTime HCV Genotype II.Results:The findings in this study illustrated a major prevalence of genotype 4(60%of cases).The study also showed a large number of patients(27%)identified as HCV genotype 1.Furthermore,the HCV genotype 1 testing detected subtypes 1a and 1b in a number of patients.It was also demonstrated that a limited number of hepatitis C patients had mixed genotypes and undermined genotypes.Conclusion:The study has confirmed that HCV genotype 4 has a high prevalence when compared to the rest of the identified genotypes.Furthermore,the majority of identified genotypes and subtypes were found to be linked with age more than 50 years,which might indicate an association of HCV genotype detection with age. 展开更多
关键词 HEPATITIS C HCV VIRUS GENOTYPES
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IHHNV PCR检测方法改进及广西流行株基因型分析 预览
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作者 杨慧赞 童桂香 +5 位作者 郑晓聪 谭红连 黄国秋 廖永志 韦信贤 胡庭俊 《南方农业学报》 CAS CSCD 北大核心 2019年第5期1127-1132,共6页
[目的]建立并优化传染性皮下及造血组织坏死病毒(IHHNV)检测及其基因分型的套式PCR,并确定IHH-NV在广西流行的基因型,为有效防控广西对虾传染性皮下及造血组织坏死病(IHHN)提供参考依据。[方法]在以PCR检测IHHNV现有标准的基础上,于389... [目的]建立并优化传染性皮下及造血组织坏死病毒(IHHNV)检测及其基因分型的套式PCR,并确定IHH-NV在广西流行的基因型,为有效防控广西对虾传染性皮下及造血组织坏死病(IHHN)提供参考依据。[方法]在以PCR检测IHHNV现有标准的基础上,于389F/R和309F/R两对引物扩增片段之外的绝对保守区域设计外引物IHHNV-WF/WR,优化退火温度和引物浓度后建立IHHNV检测及基因分型的套式PCR,并通过与一步法PCR对比以验证其优越性;采用建立的套式PCR对546株2010 2018年收集的IHHNV广西流行株进行基因型分析,确定IHHNV在广西流行的基因型。[结果]优化后的第一轮PCR反应体系20.0 L:2×F8 FastLong PCR MasterMix 10.0 μL,上、下游引物(20 μmol/L)各0.8 μL,DNA模板2.0 μL,无核菌酶灭菌水补足至20.0 μL。扩增程序:94.0 ℃预变性3 min;94.0 ℃ 10 s,59.0 ℃ 15 s,72.0 ℃ 15 s,进行35个循环;72.0 ℃延伸5 min。套式PCR检测IHHNV的灵敏度较一步法PCR提高100倍;对100份IHHNV阳性临床样品的检测结果与一步法PCR的检测结果一致,符合率达100%。从546株IHHNV广西流行株中均能扩增获得309 bp的目的条带,全部为感染型(基因1型和基因2型)。[结论]在PCR检测IHHNV现有标准基础上建立的IHHNV检测及基因分型套式PCR,其灵敏度较一步法PCR提高100倍,尤其适用于检测IHHNV含量低的样品,可为疫病监测及进出口检疫提供更敏感的技术手段。当前广西流行的IHHNV均为感染型(基因1型和基因2型)。 展开更多
关键词 对虾 传染性皮下及造血组织坏死病毒(IHHNV) 套式PCR 基因型
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不同基因型玉米品种灌浆及高产早熟特性研究 预览
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作者 洪德峰 张学舜 +6 位作者 马俊峰 卫晓轶 王稼苜 魏锋 马毅 唐振海 彭东 《中国农学通报》 2019年第30期16-21,共6页
为探明不同基因型玉米品种产量、熟期及籽粒灌浆之间的差异,为玉米机收熟期选择提供理论依据。选择‘新单68’、‘新单61’、‘郑单958’为研究材料,研究生育期、品质及籽粒鲜重、体积、干重的变化,并对其产量及产量构成因素进行比较分... 为探明不同基因型玉米品种产量、熟期及籽粒灌浆之间的差异,为玉米机收熟期选择提供理论依据。选择‘新单68’、‘新单61’、‘郑单958’为研究材料,研究生育期、品质及籽粒鲜重、体积、干重的变化,并对其产量及产量构成因素进行比较分析。结果表明,‘新单68’亲本早熟性好,籽粒粗淀粉含量高,生育期短,‘新单61’和‘新单68’的籽粒体积均小于‘郑单958’;‘新单61’、‘新单68’的籽粒体积没有明显差异。‘郑单958’籽粒干重自授粉平稳增加,‘新单61’、‘新单68’授粉24天内,干重快速增加,之后增速放缓。‘新单68’产量显著高于‘郑单958’,‘新单68’和‘新单61’的含水量显著低于‘郑单958’。总而言之,‘新单68’生育期短,粗淀粉含量高,后期灌浆快,穗轴较细,说明‘新单68’籽粒容易脱水,适宜机械化收获籽粒。 展开更多
关键词 基因型 玉米 灌浆速率 高产 早熟
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Prevalence and Genotypes of Rotavirus A and Human Adenovirus among Hospitalized Children with Acute Gastroenteritis in Fujian, China, 2009-2017 预览
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作者 WU Bing Shan HUANG Zhi Miao +4 位作者 WENG Yu Wei CHEN Feng Qin ZHANG Yun Lin LIN Wei Dong YU Ting Ting 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第3期210-214,共5页
Gastroenteritis is an infectious diarrhea that has been considered as an important cause of hospitalizations and death in children aged < 5 years, particularly in developing countries. Unsanitary water, contaminate... Gastroenteritis is an infectious diarrhea that has been considered as an important cause of hospitalizations and death in children aged < 5 years, particularly in developing countries. Unsanitary water, contaminated food, poor hygiene, and inadequate disposal of waste and feces are all risk factors for gastroenteritis, resulting in the higher incidence in developing countries. Gastroenteritis is generally caused by viral infections, among which rotavirus (RV) infections have been reported to be the most common, especially among young children aged < 5 years with acute gastroenteritis in Asia and Africa[1]. Other viruses associated with acute gastroenteritis include human Adenovirus (HAdV), Norovirus, Sapovirus (SaV), human Astrovirus (HAstV), and Aichi virus. Recent research has reported that adenovirus types 40 and 41, belonging to species F, cause gastroenteritis and were therefore termed as enteric adenoviruses. In addition, non-enteric HAdV species such as A, B, C, and D have been associated with diarrheal. 展开更多
关键词 PREVALENCE and GENOTYPES ROTAVIRUS A ACUTE GASTROENTERITIS
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广州地区尖锐湿疣患者HPV感染情况及其基因分型
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作者 方伟祯 蔡振华 +3 位作者 古文深 丁睿 潘昆贻 段朝晖 《中国微生态学杂志》 CAS CSCD 2019年第6期673-676,共4页
目的分析广州地区尖锐湿疣患者人乳头瘤病毒感染状况及其基因型分布情况,为临床早期诊断和治疗提供依据。方法采用基因芯片技术对2013年1月至2017年12月在我院皮肤科就诊的421例不同年龄性别的尖锐湿疣患者进行HPV基因检查,分析HPV-DNA... 目的分析广州地区尖锐湿疣患者人乳头瘤病毒感染状况及其基因型分布情况,为临床早期诊断和治疗提供依据。方法采用基因芯片技术对2013年1月至2017年12月在我院皮肤科就诊的421例不同年龄性别的尖锐湿疣患者进行HPV基因检查,分析HPV-DNA亚型分布状况。结果 421例受检者中共检出HPV阳性者395例,阳性率为93.82%,其中高危型HPV感染阳性率为39.43%(166/421)。在被检测的21个HPV亚型中,最常见类型依次为6型(45.13%,190/421)、11型(29.22%,123/421)和16型(9.03%,38/421),其中女性高危型HPV感染率为23.28%(98/421),明显高于男性的16.15%(68/421)。HPV阳性者中多重感染率为32.54%(137/421),以二重感染最常见(19.71%,83/421)。不同年龄段人群HPV阳性率有差异,21~30岁人群HPV阳性率最高(47.74%,201/421)。结论广州地区尖锐湿疣患者HPV最常见的亚型是HPV6、HPV11和HPV16型,高危型HPV感染率较高并多以混合感染形式存在。分析HPV基因亚型是临床尖锐湿疣诊断和疗效观察的理想方法。 展开更多
关键词 尖锐湿疣 人乳头状瘤病毒 感染 基因分型
乙型肝炎病毒B和C基因型S蛋白特异性CTL表位保守性分析 预览
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作者 陈庆新 李文姝 +4 位作者 陈卓艳 何佳莉 汪文寰 林茂 叶菊秀 《温州医科大学学报》 CAS 2019年第4期253-257,共5页
目的:分析国内流行乙型肝炎病毒(HBV)基因型S区基因编码蛋白的特异性CTL表位保守性,发现稳定且特异性的CTL表位。方法:设计HBVS基因特异性引物,克隆S基因并构建到pIRES载体;利用3种表位分析方法对HBVB和C基因型S区基因编码蛋白特异性CT... 目的:分析国内流行乙型肝炎病毒(HBV)基因型S区基因编码蛋白的特异性CTL表位保守性,发现稳定且特异性的CTL表位。方法:设计HBVS基因特异性引物,克隆S基因并构建到pIRES载体;利用3种表位分析方法对HBVB和C基因型S区基因编码蛋白特异性CTL表位进行分析筛选,对候选CTL表位用BLAST搜索HBV蛋白库,分析候选CTL表位在HBVB和C基因型中的保守性。结果:成功克隆HBVS基因并构建真核表达载体;根据设定条件,利用生物信息学分析方法对S区基因编码蛋白进行分析共筛选到17个CTL表位,其中已经鉴定6个,未鉴定表位11个;保守性分析发现,在B和C基因型中具有较高保守性的有6个(>80%),均是未鉴定的表位,其余表位(包括6个已鉴定)的保守性较低。结论:国内流行HBV基因型间S区基因编码蛋白的特异性CTL表位存在较大差异性,共筛选到6个保守性较高的特异性CTL表位可用于进一步的免疫学研究。 展开更多
关键词 HBV 基因型 CTL表位 保守性分析
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Evapotranspiration, Grain Yield, and Water Productivity of Spring Oat (Avena sativa L.) under Semiarid Climate 预览
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作者 Koffi Djaman Michael O’Neill +2 位作者 Curtis Owen Komlan Koudahe Kevin Lombard 《农业科学(英文)》 2018年第9期1188-1204,共17页
Spring oat (Avena sativa) is produced for grain, hay, and green manure and can be integrated into a cropping system as a cover crop. Twenty-eight oat genotypes (G1, G2, G3, …., G28), selected for their adaptability t... Spring oat (Avena sativa) is produced for grain, hay, and green manure and can be integrated into a cropping system as a cover crop. Twenty-eight oat genotypes (G1, G2, G3, …., G28), selected for their adaptability to the Southwestern United States, were evaluated for their yield performance under sprinkler irrigation during four growing seasons (2005-2008) at the Agricultural Science Center at Farmington, New Mexico State University. The genotypes were arranged in randomized complete blocs design with four replications. Irrigation scheduling was based on evapotranspiration and the depletion criterion of 40% to 45% total available water (TAW) was practiced to prevent the plants from experiencing any water stress. Crop evapotranspiration estimated by the FAO crop coefficient and reference evapotranspiration approach was low about 2 mm/day during crop initial stage and increased with plant growth and reached the maximum during crop mid-season or reproductive stage. It decreased during crop late season. Daily crop evapotranspiration varied from 0.5 to 12.6 mm in 2008 and the seasonal Spring oat evapotranspiration varied from 535.8 to 591 mm. Averaged across the four growing seasons, oat evapotranspiration was 570.4 mm. The results showed that Spring oat plant height varied significantly with genotypes and ranged from 59.1 to 100.8 cm. Oat grain yield significantly varied with years and genotypes. Grain yield varied from 3386 to 6498 kg/ha and average yield was 4245, 4265, 5477, and 4025 kg/ha during the 2005, 2006, 2007 and 2008, respectively. The best performing genotypes were G1, G2, G7, G19, G20, G21 and G23 with average yield greater than 4800 kg/ha while G3, G13, G17 and G27 showed the lowest yield among the genotypes. Oat crop water use efficiency (CWUE) varied with genotype and years and ranged from 0.53 to 1.07 kg/m3 and averaged 0.65, 0.78, 0.91 and 0.70 kg/m3 in 2005, 2006, 2007 and 2008, respectively. The highest CWUE was achieved by G19 and the lowest CWUE was obtained by G13. Irrigation water use effi 展开更多
关键词 SPRING OAT Genotypes Evaluation Grain Yield Water Productivity High Elevation SEMIARID Climate
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Molecular Diversity Analysis of Some Chilli (<i>Capsicum</i>spp.) Genotypes Using SSR Markers 预览
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作者 Afsana Sharmin Md. Ekramul Hoque +1 位作者 Md. Masudul Haque Fahima Khatun 《美国植物学期刊(英文)》 2018年第3期368-379,共12页
Chilli belongs to the genus Capsicum which possesses enormous wealth of genetic diversity. Extent of genetic diversity determines the success level of crop improvement programme. Simple sequence repeats (SSRs) are the... Chilli belongs to the genus Capsicum which possesses enormous wealth of genetic diversity. Extent of genetic diversity determines the success level of crop improvement programme. Simple sequence repeats (SSRs) are the most widely used marker system for molecular diversity analysis especially in cultivated species. The aim of our present study was to assess the molecular genetic diversity of 20 local chilli genotypes of Bangladesh using SSR markers. Genomic DNA was extracted from young leaves and PCR reactions were performed. Eleven SSR primers were used in PCR amplification. Total 10 alleles were detected for the five polymorphic SSR loci, with a mean of 2.00 alleles per primer. Gene diversity ranged from 0.333 to 1.00 with an average of 0.567. Polymorphic Information Content (PIC) values of the SSR primers ranged from 0.255 to 0.500 with an average value of 0.371. The similarity index matrix ranged from 0.00 to 1.000. It was highest in several germplasms viz. Pop-2 vs Pop-18;Pop-3 vs Pop-5 vs Pop-19 vs Pop-20 and the lowest in the germplasm Pop-8 vs Pop-18. Dendrogram based on Nei’s genetic distance using Unweighted Pair Group Method of Arithmetic Means (UPGMA) indicated the segregation of 20 chilli genotypes into two main clusters. The SSR markers showed genetic variability in the studied pepper genotypes and they are powerful tools for estimating molecular diversity of chilli. The findings of the present study have potential applications in future breeding programme for the genetic improvement of chilli. 展开更多
关键词 CAPSICUM MOLECULAR Diversity GENOTYPES SSR Markers Polymorphism
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Evaluating the Optimum Transplanting Time for Different Coarse Rice Genotypes under Semi-Arid Conditions of Faisalabad 预览
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作者 Muhammad Ahtisham Tahir Muhammad Ahmad Arain +7 位作者 Saba Durrani Abdul Shakoor Ahtsham Bilal Nasir Ali Muhammad Ishfaq Umer Farooq Siraj Ahmed Muhammad Irfan 《农业科学(英文)》 2018年第1期69-77,共9页
Continuously changing climate and availability of different rice genotypes make it necessary to find optimum time of sowing as well as suitable variety for cultivation to get maximum productivity under a specific set ... Continuously changing climate and availability of different rice genotypes make it necessary to find optimum time of sowing as well as suitable variety for cultivation to get maximum productivity under a specific set of climatic conditions. A field study was carried out to search out the suitable rice transplanting time for four different coarse genotypes under the semi-arid environment of Faisalabad. The experiment was conducted at Agronomic Research Area, University of Agriculture, Faisalabad and was laid out in randomized complete block design (RCBD) with split plot arrangement keeping transplanting time in main plots while rice genotypes in subplots. Variability among treatments was measured by Fisher’s ANOVA (P ≤ 5%) and LSD test was applied to compare the differences among treatments’ means. The ANOVA indicated statistically significant differences among genotypes as well as transplanting dates irrespective of all studied traits while interactive effects of both were found to be non-significant. NIBGE-1 performed best with maximum paddy yield of 6.05 t/ha while KSK-434 performed poor with paddy yield of 2.78 t/ha. Increased paddy yield and yield related parameters of all rice genotypes were recorded where transplantation was done on 25th of June. Generally, paddy yield decreased with delaying the transplanting time. The results suggested that NIBGE-1 can perform better under the semi-arid conditions of Faisalabad and last week of June might be the optimum time for nursery transplantation. It can also be further elucidated that late transplanting causes yield reduction which could not be recommended among farmers. 展开更多
关键词 Coarse Rice GENOTYPES TRANSPLANTING Time PADDY Yield SEMI-ARID
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Effect of Drought Stress on Yield Performance of Parental Chickpea Genotypes in Semi-arid Tropics 预览
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作者 Ruth Muruiki Paul Kimurto +3 位作者 Vincent Vandez Rao Gangarao Said Silim Moses Siambi 《生命科学:英文版(ISSN1934-7391)》 2018年第3期159-168,共10页
关键词 半干旱 遗传型 应力 父母 热带 性能 计算中心 肯尼亚
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