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A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells
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作者 Tun Tang Yu-Qi Chen +6 位作者 Bo-Shi Fu Zhi-Yong He Heng Xiao Fan Wu Jia-Qi Wang Shao-Ru Wang Xiang Zhou 《中国化学快报:英文版》 SCIE CAS CSCD 2016年第4期540-544,共5页
In this study, a resorufin derivative RTP-1, which is a novel fluorescent ‘‘turn-on’’ probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester ... In this study, a resorufin derivative RTP-1, which is a novel fluorescent ‘‘turn-on’’ probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester group of the probe by hydrazine led to a prominent enhancement of fluorescent intensity, as well as a remarkable color change from colorless to pink, which could be distinguished by naked eye. The fluorescence enhancement showed decent linear relationship with hydrazine concentration ranging from 0 to 50 mmol/L, with a detection limit of 0.84 mmol/L. The specificity of RTP-1 for hydrazine to a number of metal ions, anions and amines is satisfactory. The sensing mechanism of RTP-1 and hydrazine was evaluated by HPLC, ESI mass spectrometry and density functional theory(DFT).Moreover, we have utilized this fluorescent probe for imaging hydrazine in living cells, and the fluorescence was clearly observed when the cells were incubated with hydrazine(100 mmol/L) for 30 min. 展开更多
关键词 选择性检测 活细胞成像 荧光探针 水合肼 CYP2E1 应用 密度泛函理论 HPLC法
金属硫蛋白的抗休克作用及其与内皮素的关系 预览 被引量:1
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作者 张连元 董淑云 +6 位作者 杨军 佟利家 汤健 唐朝枢 杨晔 石湘云 程时 《基础医学与临床》 CSCD 1993年第1期 67-70,共4页
为探讨金属硫蛋白(MT)的细胞保护机制及与内皮素(ET)的关系,在大鼠止血带休克模型上观察到MT明显改善动物休克状态的同时显著抑制其血浆ET含量的增加。离体实验发现MT呈剂量依赖地抑制ET的缩血管作用和抑制血管紧张素Ⅱ刺激ET的释放。提... 为探讨金属硫蛋白(MT)的细胞保护机制及与内皮素(ET)的关系,在大鼠止血带休克模型上观察到MT明显改善动物休克状态的同时显著抑制其血浆ET含量的增加。离体实验发现MT呈剂量依赖地抑制ET的缩血管作用和抑制血管紧张素Ⅱ刺激ET的释放。提示MT可以通过抑制ET的释放和拮抗ET的缩血管效应,发挥其内源性抗损伤物质的作用。 展开更多
关键词 金属硫蛋白 内皮素 再灌注损伤
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