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Macrophage migration inhibitory factor as a potential prognostic factor in gastric cancer 预览 被引量:1
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作者 Long-JunHe DanXie +4 位作者 Pin-JinHu Yi-JiLiao Hai-XiaDeng Hsiang-FuKung Sen-LinZhu 《世界胃肠病学杂志:英文版》 SCIE CAS 2015年第34期9916-9926,共11页
AIM:To investigate macrophage migration inhibitory factor(MIF) expression and its clinical relevance in gastric cancer,and effects of MIF knockdown on proliferation of gastric cancer cells. METHODS:Tissue microarray c... AIM:To investigate macrophage migration inhibitory factor(MIF) expression and its clinical relevance in gastric cancer,and effects of MIF knockdown on proliferation of gastric cancer cells. METHODS:Tissue microarray containing 117 samples of gastric cancer and adjacent non-cancer normal tissues was studied for MIF expression by immunohistochemistry(IHC) semiquantitatively,and the association of MIF expression with clinical parameters was analyzed. MIF expression in gastric cancer cell lines was detected by reverse transcriptionpolymerase chain reaction(RT-PCR) and Western blot. Two pairs of si RNA targeting the MIF gene(MIF si-1 and MIF si-2) and one pair of scrambled si RNA as a negative control(NC) were designed and chemically synthesized. All si RNAs were transiently transfected in AGS cells with OligofectamineTM to knock down the MIF expression,with the NC group and mock group(OligofectamineTM alone) as controls. At 24,48,and 72 h after transfection,MIF m RNA was analyzed by RTPCR,and MIF and proliferating cell nuclear antigen(PCNA) proteins were detected by Western blot.The proliferative rate of AGS cells was assessed by methylthiazolyl tetrazolium(MTT) assay and colony forming assay.RESULTS:The tissue microarray was informative for IHC staining,in which the MIF expression in gastric cancer tissues was higher than that in adjacent noncancer normal tissues(P < 0.001),and high level of MIF was related to poor tumor differentiation,advanced T stage,advanced tumor stage,lymph node metastasis,and poor patient survival(P < 0.05 for all). After si RNA transfection,MIF m RNA was measured by real-time PCR,and MIF protein and PCNA were assessed by Western blot analysis. We found that compared to the NC group and mock group,MIF expression was knocked down successfully in gastric cancer cells,and PCNA expression was downregulated with MIF knockdown as well. The cell counts and the doubling times were assayed by MTT 4 d after transfection,and colonies formed were assayed by colony forming assay 10 d after transfection; a 展开更多
关键词 MACROPHAGE MIGRATION INHIBITORY FACTOR Proliferati
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巨噬细胞游走抑制因子在卵巢癌侵袭中的作用与意义 预览 被引量:2
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作者 武鸿美 朱森林 +2 位作者 贺龙君 刘艳辉 谢丹 《癌症:英文版》 SCIE CAS CSCD 北大核心 2009年第10期1054-1060,共7页
背景与目的:巨噬细胞游走抑制因子(macrophage migration inhibitory factor,MIF)与肿瘤的恶性进展密切相关。本研究探讨MIF基因对卵巢癌HO-8910和OVCAR-3细胞侵袭和增殖能力的影响及其在卵巢癌组织中表达的意义。方法:瞬时转染小... 背景与目的:巨噬细胞游走抑制因子(macrophage migration inhibitory factor,MIF)与肿瘤的恶性进展密切相关。本研究探讨MIF基因对卵巢癌HO-8910和OVCAR-3细胞侵袭和增殖能力的影响及其在卵巢癌组织中表达的意义。方法:瞬时转染小干扰RNA(small interfering RNA,siRNA)靶向敲除MIF基因,RT-PCR和Westernblot检测MIF在mRNA和蛋白水平的表达,通过体外迁移、侵袭实验和噻唑蓝比色法(MTT)分析MIF对HO-8910和OVCAR-3细胞迁移、侵袭和增殖能力的影响;免疫组织化学检测MIF蛋白在卵巢癌组织中的表达情况。结果:与阴性对照组细胞比较,瞬时转染MIF siRNA的HO-8910和OVCAR-3细胞中MIF基因表达水平明显降低。MTT结果显示,转染MIFsiRNA的两株卵巢癌细胞增殖率显著低于阴性对照组(P〈0.05)。转染MIFsiRNA的HO-8910细胞穿膜细胞数(MIF—sil:48.0±7.3;MIF—si2:38.0±3.6)与阴性对照组(78.0±8.5)比较差异有统计学意义(P〈0.05),其穿过铺了Matrigel膜的细胞数(MIF—sil:35.0±5.0:MIF—si2:30.0±5.6)也显著少于阴性对照组(P〈0.05)。同样,转染了MIF siRNA的OVCAR-3细胞穿膜细胞数(MIF—sil:40.0±4.5:MIF—si2:42.0±3.0)与阴性对照组(65±2.1)比较,差异也有统计学意义(P〈0.05),其穿过铺了Matrigel膜的细胞数(MIF—sil:25.0±3.0;MIF—si2:27.0±3.4)也明显低于阴性对照组(P〈0.05)。53.6%(37/69)卵巢癌组织中有MIF蛋白表达,MIF蛋白表达与肿瘤临床分期呈显著正相关(P〈0.01)。结论:MIF基因可能通过促进肿瘤细胞的增殖和侵袭能力在卵巢癌的发生发展中发挥重要作用.有可能成为分子靶向治疗卵巢癌的潜在靶点。 展开更多
关键词 巨噬细胞游走抑制因子 RNA干扰 卵巢肿瘤 迁移 侵袭
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幽门螺杆菌疫苗接种小鼠产生免疫后胃炎的影响因素 预览 被引量:1
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作者 陈洁 陈旻湖 +1 位作者 王锦辉 朱森林 《世界华人消化杂志》 CAS 北大核心 2006年第23期2275-2280,共6页
目的:研究幽门螺杆菌(H pylori)疫苗接种小鼠产生免疫后胃炎的影响因素. 方法:将H pylori疫苗免疫C57BL/6和BALB/c的小鼠,观察攻击后胃黏膜H pylori定植和炎症情况.将H pylori疫苗免疫C57BL/6小鼠,然后予不同菌量的H pylor... 目的:研究幽门螺杆菌(H pylori)疫苗接种小鼠产生免疫后胃炎的影响因素. 方法:将H pylori疫苗免疫C57BL/6和BALB/c的小鼠,观察攻击后胃黏膜H pylori定植和炎症情况.将H pylori疫苗免疫C57BL/6小鼠,然后予不同菌量的H pylori攻击,观察胃黏膜H pylori定植和炎症情况.将H pylori疫苗经口和经腹腔免疫C57BL/6小鼠,观察攻击后胃黏膜Ⅳ砌定植和炎症情况.对感染H pylori C57BL/6小鼠予H pylori疫苗治疗,观察治疗免疫后胃黏膜Ⅳ砌定植和炎症情况. 结果:不同品系的小鼠免疫保护程度无明显差异,但C57BL/6小鼠免疫后胃炎重于BALB/c小鼠.接受不同攻击菌量的小鼠保护程度无明显差异,但大的攻击菌量可诱导更严重的免疫后炎症.不同免疫途径诱导的免疫保护程度及攻击后不同时间点的炎症程度均无显著性差异.治疗性免疫导pylori定植明显降低,同时也引发更为严重的胃炎. 结论:在不同的免疫宿主、免疫途径和治疗性免疫中均存在免疫后胃炎.免疫后胃炎的强弱程度受免疫宿主和攻击菌量的影响. 展开更多
关键词 幽门螺杆菌 疫苗 免疫后胃炎 小鼠
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