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Establishment and application of distant hybridization technology in fish
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作者 Shi Wang Chenchen Tang +11 位作者 Min Tao Qinbo Qin Chun Zhang Kaikun Luo Rurong Zhao Jing Wang Li Ren Jun Xiao Fangznou Hu Rong Zhou Wei Duan Shaojun Liu 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第1期22-45,共24页
Hybridization is widely used. However, for a long time, systematic theories and technologies related to hybridization in fish have been lacking. In this study, through long-term systematic research, we investigated an... Hybridization is widely used. However, for a long time, systematic theories and technologies related to hybridization in fish have been lacking. In this study, through long-term systematic research, we investigated and obtained the main rules regarding inheritance and reproduction related to fish distant hybridization. Furthermore, we established one-step and multistep breeding technologies that were suitable for interspecific hybridization and intraspecific hybridization. Simultaneously, we used these two breeding technologies to produce a batch of diploid fish lineages and tetraploid fish lineages and improved fishes. In addition, we widely discuss the methods, technologies and results of hybridization breeding, referring to the domestic and foreign literature on fish hybridization. We hope that this paper will be beneficial for the research and application of fish hybrid breeding. 展开更多
关键词 FISH DISTANT HYBRIDIZATION INTRASPECIFIC HYBRIDIZATION breeding TECHNOLOGY LINEAGE
Targeted disruption of tyrosinase causes melanin reduction in Carassius auratus cuvieri and its hybrid progeny
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作者 Qingfeng Liu Yanhua Qi +9 位作者 Qiuli Liang Jia Song Junmei Liu Wuhui Li Yuqin Shu Min Tao Chun Zhang Qinbo Qin Jing Wang Shaojun Liu 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第9期1194-1202,共9页
The white crucian carp(Carassius auratus cuvieri,WCC) not only is one of the most economically important fish in Asia,characterized by strong reproductive ability and rapid growth rates,but also represents a good germ... The white crucian carp(Carassius auratus cuvieri,WCC) not only is one of the most economically important fish in Asia,characterized by strong reproductive ability and rapid growth rates,but also represents a good germplasm to produce hybrid progenies with heterosis.Gene knockout technique provides a safe and acceptant way for fish breeding.Achieving gene knockout in WCC and its hybrid progeny will be of great importance for both genetic studies and hybridization breeding.Tyrosinase(TYR) is a key enzyme in melanin synthesis.Depletion of tyr in zebrafish and mice results in mosaic pigmentation or total albinism.Here,we successfully used CRISPR-Cas9 to target tyr in WCC and its hybrid progeny(WR) derived from the cross of WCC(♀) and red crucian carp(Carassius auratus red var.,RCC,♂).The level of TYR protein was significantly reduced in mutant WCC.Both the mutant WCC and the mutant WR showed different degrees of melanin reduction compared with the wild-type sibling control fish,resulting from different mutation efficiency ranging from 60% to 90%.In addition,the transcriptional expression profiles of a series of pivotal pigment synthesis genes,i.e.tyrp1,mitfa,mitfb,dct and sox10,were down-regulated in tyr-CRISPR WCC,which ultimately caused a reduction in melanin synthesis.These results demonstrated that tyr plays a key role in melanin synthesis in WCC and WR,and CRISPR-Cas9 is an effective tool for modifying the genome of economical fish.Furthermore,the tyr-CRISPR models could be valuable in understanding fundamental mechanisms of pigment formation in non-model fish. 展开更多
关键词 white crucian CARP HYBRIDIZATION CRISPR-Cas9 TYROSINASE PIGMENTATION
Comparison of diploid and triploid Carassius auratus provides insights into adaptation to environmental change
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作者 Li Ren Xin Gao +8 位作者 Conghui Yang Hui Tan Jialin cui Shi Wang Wuhui Li Chun Zhang Min Tao Qinbo Qin Shaojun Liu 《中国科学:生命科学英文版》 SCIE CAS CSCD 2018年第11期1407-1419,共13页
Focusing on adaptation of aquatic organisms, especially fish, can help elucidate complex dynamics in freshwater ecology. The differences in genetic and epigenetic regulation between diploid and triploid Carassius aura... Focusing on adaptation of aquatic organisms, especially fish, can help elucidate complex dynamics in freshwater ecology. The differences in genetic and epigenetic regulation between diploid and triploid Carassius auratus affect survival under eutrophication. To identify the underlying mechanisms that lead to better adaption of triploids than diploids, we compared mRNA and microRNA(miRNA) expressions in liver tissue of diploid and triploid individuals obtained from the Dongting lake water system in central China. Differential expression analysis revealed that 566 transcripts were significantly up-regulated, whereas758 were down-regulated in triploids; of these differentially expressed transcripts, 33 transcripts including cacna1 d, nfkb2, hspa1 and fgfr4 were involved in the MAPK signaling pathway, and eight transcripts were determined to be regulated by seven miRNAs. Additionally, four of 25 differential expressed(DE) transcripts(mhc1, irf7, nfkb2 and pik3 c) involving the viral carcinogenesis pathway were regulated by four miRNAs. Furthermore, genetic polymorphisms analysis showed that more heterozygous mutations were detected in triploids than diploids. The d N/d S results revealed that 21 genes were under positive selection(d N/d S>1) in C. auratus complex. We hypothesize that these changes related to genetic and epigenetic regulation may be caused by abiotic stresses, and facilitate adaptation to environmental changes. 展开更多
关键词 TRIPLOID ADAPTING positive selection differential expression
A comparative study of distant hybridization in plants and animals
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作者 Jie Chen Mi Luo +7 位作者 Shengnan Li Min Yao Xiaolan Ye Wei Duan Chun Zhang Qinbo Qin Jun Xiao Shaojun Liu 《中国科学:生命科学英文版》 SCIE CAS CSCD 2018年第3期285-309,共25页
关键词 动物 植物 杂交 生物特征 基因变化 新种类 混血儿 生长特点
The chimeric genes in the hybrid lineage of Carassius auratus cuvieri(♀)×Carassius auratus red var.(♂)
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作者 Qingfeng Liu Yanhua Qi +11 位作者 Qiuli Liang Xiujuan Xu Fangzhou Hu Jing Wang Jun Xiao Shi Wang Wuhui Li Min Tao Qinbo Qin Rurong Zhao Zhanzhou Yao Shaojun Liu 《中国科学:生命科学英文版》 SCIE CAS CSCD 2018年第9期1079-1089,共11页
关键词 基因变化 var 混合 混血儿 新陈代谢 免疫系统 染色体 遗传型
Autotriploid origin of Carassius auratus as revealed by chromosomal locus analysis
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作者 Qinbo Qin Juan Wang +2 位作者 Min Hu Shengnan Huang Shaojun Liu 《中国科学:生命科学英文版》 SCIE CAS CSCD 2016年第6期622-626,共5页
在 Dongting 水系统, Carassius auratus (鲫鱼鲤鱼) 建筑群被双形式的共存描绘(2n=100, 2nCC ) 并且多倍体形式。Chromosomal 和 karyotypic 分析建议了多倍体 C。auratus 有一三倍性(3n=150, 3nCC ) 并且 tetraploid 起源(4n=200 )... 在 Dongting 水系统, Carassius auratus (鲫鱼鲤鱼) 建筑群被双形式的共存描绘(2n=100, 2nCC ) 并且多倍体形式。Chromosomal 和 karyotypic 分析建议了多倍体 C。auratus 有一三倍性(3n=150, 3nCC ) 并且 tetraploid 起源(4n=200 ) 分别地。然而,有直接基因的证据的缺乏支持这个结论。在这份报纸, 5S rDNA chromosomal 地点的分析表明 3nCC 具有三倍性的起源。种类特定的 chromosomal 着丝点地点的分析表明 3nCC 个人拥有 C 的三个集合。导出 auratus 的染色体。我们的结果提供建议有 150 个染色体的个人具有在 C 以内的 autotriploid 起源的直接细胞发生的证据。auratus 建筑群。它标记重要贡献到 polyploidization 和脊椎动物的进化的学习。 展开更多
关键词 染色体核型分析 同源三倍体 位点分析 鲫鱼 起源 染色体位点 多倍体 成因分析
Research advances in animal distant hybridization
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作者 ZHANG ZhuoHui CHEN Jie +6 位作者 LI Ling TAO Min ZHANG Chun QIN QinBo XIAO Jun LIU Yun LIU ShaoJun 《中国科学:生命科学英文版》 SCIE CAS 2014年第9期889-902,共14页
Distant hybridization refers to crosses between two different species or higher-ranking taxa that enables interspecific genome transfer and leads to changes in phenotypes and genotypes of the resulting progeny.If prog... Distant hybridization refers to crosses between two different species or higher-ranking taxa that enables interspecific genome transfer and leads to changes in phenotypes and genotypes of the resulting progeny.If progeny derived from distant hybridization are bisexual and fertile,they can form a hybrid lineage through self-mating,with major implications for evolutionary biology,genetics,and breeding.Here,we review and summarize the published literature,and present our results on fish distant hybridization.Relevant problems involving distant hybridization between orders,families,subfamilies,genera,and species of animals are introduced and discussed,with an additional focus on fish distant hybrid lineages,genetic variation,patterns,and applications.Our review serves as a useful reference for evolutionary biology research and animal genetic breeding. 展开更多
关键词 动物遗传育种 远缘杂交 进化生物学 分类单元 基因转移 遗传变异 基因型 遗传学
Polyploid organisms
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作者 SONG Can LIU ShaoJun XIAO Jun HE WeiGuo ZHOU Yi QIN QinBo ZHANG Chun LIU Yun 《中国科学:生命科学英文版》 SCIE CAS 2012年第4期301-311,共11页
Polyploids are organisms with three or more complete chromosome sets. Polyploidization is widespread in plants and animals, and is an important mechanism of speciation. Genome sequencing and related molecular systemat... Polyploids are organisms with three or more complete chromosome sets. Polyploidization is widespread in plants and animals, and is an important mechanism of speciation. Genome sequencing and related molecular systematics and bioinformatics studies on plants and animals in recent years support the view that species have been shaped by whole genome duplication during evolution. The stability of polyploids depends on rapid genome recombination and changes in gene expression after formation. The formation of polyploids and subsequent diploidization are important aspects in long-term evolution. Polyploids can be formed in various ways. Among them, hybrid organisms formed by distant hybridization could produce unreduced gametes and thus generate offspring with doubled chromosomes, which is a fast, efficient method of polyploidization. The formation of fertile polyploids not only promoted the interflow of genetic materials among species and enriched the species diversity, but also laid the foundation for polyploidy breeding. The study of polyploids has both important theoretical significance and valuable applications. The production and application of polyploidy breeding have brought remarkable economic and social benefits. 展开更多
关键词 多倍体育种 有机体 基因组测序 染色体组 动物物种 生物信息学 分子系统学 未减数配子
The formation of improved tetraploid population of red crucian carp × common carp hybrids by androgenesis
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作者 DUAN Wei QIN QinBo CHEN Song LIU ShaoJun WANG Jing ZHANG Chun SUN YuanDong LIU Yun 《中国科学:生命科学英文版》 SCIE CAS 2007年第6期753-761,共9页
Bisexual fertile diploid androgenetic individuals (A0) (2n=100) were formed by androgenesis. In this way, the diploid spermatozoa from male allotetraploid hybrids (AT) (4n=200) of red crucian carp (Carassius auratus r... Bisexual fertile diploid androgenetic individuals (A0) (2n=100) were formed by androgenesis. In this way, the diploid spermatozoa from male allotetraploid hybrids (AT) (4n=200) of red crucian carp (Carassius auratus red var.) (♀) × common carp (Cyprinus carpio L.) (♂) were used to fertilize the UV-treated haploid eggs of goldfish (Carassius auratus), and living androgenetic diploid fish were developed. The A0 became sexually mature at the age of 2 years, and they fertilized with each other to form their offspring (A1). In this study, we observed the chromosomal number, gonadal structure and appearance of A1 fish. The results are as follows: (1) In A1, there were 85% tetraploids (A1-4n), 10% triploids (A1-3n) and 5% diploids (A1-2n), suggesting that diploid A0 could produce diploid gametes. It was concluded that the formation of diploid gametes generated from diploid A0 was probably related to the mechanism of pre-meiotic endoreduplication. (2) Among A1, only A1-4n possessed normal ovaries and testes. The mature males of A1-4n produced white semen. Under the electron microscope, the head of diploid sperm generated by A1-4n was bigger than that of haploid sperm generated by red crucian carp. In the testes of the A1-4n, there were many mature normal spermatozoa with a head bearing plasma membrane and a tail having the typical structure of '9+2' microtubules. Between the head and the tail, there were some mitochondria. The ovaries of A1-4n developed well and mainly contained Ⅱ, Ⅲ and Ⅳ-stage oocytes. The Ⅳ-stage oocytes were surrounded by inner and outer follicular cells. The micropyle was observed on the oolemma of follicular cells. There were abundant yolks and plenty of endoplasmic reticulum in the cytoplasm of Ⅳ-stage oocytes. Because A1-2n and A1-3n were distant crossing diploid hybrids and triploid hybrids respectively, they possessed abnormal gonads, and no mature semen and eggs were observed. (3) Compared with allotetraploids, the A1-4n fish not only had advantages such as fast growth rate and 展开更多
关键词 ALLOTETRAPLOID hybrid DIPLOID sperm HAPLOID egg androgenesis DIPLOID ANDROGENETIC individuals
地表水文过程与地下水动力过程耦合模拟及应用 预览 被引量:4
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作者 凌敏华 陈喜 +2 位作者 程勤波 薛显武 秦年秀 《水文》 CSCD 北大核心 2011年第6期 8-13,共6页
地表水和地下水是水循环系统不可缺少的部分,对相互作用与反馈的模拟计算有助于水资源精准评价。在非饱和带数值计算模型(UZF1)与模块化地下水动力模型(MODFLOW2005)耦合的基础上构建了一个地表水文过程与地下水动力过程耦合数值... 地表水和地下水是水循环系统不可缺少的部分,对相互作用与反馈的模拟计算有助于水资源精准评价。在非饱和带数值计算模型(UZF1)与模块化地下水动力模型(MODFLOW2005)耦合的基础上构建了一个地表水文过程与地下水动力过程耦合数值模拟模型,模型可以同时输出流域出口断面流量和地下水位的空间分布,有利地提高了模型的可靠性。将模型应用于资料条件较好的临涣集流域,从模拟结果分析来看,模型可以很好地模拟流域出口流量与地下水位空间动态变化过程,具有较高的模拟精度。 展开更多
关键词 地表水文过程 地下水 UZF1 MODFLOW2005 耦合模拟
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