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秸秆还田下灌溉方式对水稻产量的影响 预览
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作者 赵宏亮 王麒 +7 位作者 曾宪楠 宋秋来 孙羽 张小明 王萍 王曼力 赵雪涵 冯延江 《农机化研究》 北大核心 2020年第6期1-7,13共8页
为明确水稻不同品种在秸秆还田和灌溉方式互作条件下的表现差异,以9个水稻品种为供试材料,在秸秆全量还田下设置4种灌溉方式,测定水稻不同品种的产量及其构成因素,进而进行相关分析和主成分分析。结果表明:4种灌溉方式下,9个水稻品种的... 为明确水稻不同品种在秸秆还田和灌溉方式互作条件下的表现差异,以9个水稻品种为供试材料,在秸秆全量还田下设置4种灌溉方式,测定水稻不同品种的产量及其构成因素,进而进行相关分析和主成分分析。结果表明:4种灌溉方式下,9个水稻品种的8个性状指标变异系数最大的为瘪粒数,其次是有效穗数、产量、结实率和穗长;产量及其构成因素间存在着显著或极显著的相关性。主成分分析表明:8个相关性状可简化为3个彼此不相关主成分因子,其所提供的信息量占全部信息量的82.81%。基于主成分的综合评价表明:龙稻14、龙稻17、龙香稻2在传统灌溉方式下表现最好,其次是间歇灌溉和控制灌溉I;中龙粳2、龙稻27、中龙粳3、龙稻7、龙稻26、龙稻22在间歇灌溉方式下表现最好,其次是传统灌溉和控制灌溉I。 展开更多
关键词 水稻 秸秆还田 灌溉方式 主成分分析
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育苗纸钵自动装盘装置的试验研究 预览
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作者 张洋 范开钧 +4 位作者 初麒 苏颖欣 刘小耿 辜松 夏红梅 《农机化研究》 北大核心 2020年第4期180-183,共4页
纸钵育苗可有效提升种苗根系的发育质量,为种苗后续分级、嫁接、移植的机械化作业打下重要技术基础。目前,国内大多企业所采用的纸钵机多为单头、半自动作业形式,缺少纸钵自动装盘设备。纸钵机制作出纸钵基质块后,需人工向穴盘装填纸钵... 纸钵育苗可有效提升种苗根系的发育质量,为种苗后续分级、嫁接、移植的机械化作业打下重要技术基础。目前,国内大多企业所采用的纸钵机多为单头、半自动作业形式,缺少纸钵自动装盘设备。纸钵机制作出纸钵基质块后,需人工向穴盘装填纸钵,作业效率低,劳动强度大;国外虽有纸钵装填机,但价格高,且无法与国产设备配套。为实现纸钵自动快速装入穴盘,完善育苗生产线,设计了一款育苗纸钵自动装盘装置。该装置与纸钵机配合作业,将规格为高度50mm纸钵装入72孔穴盘。通过装盘性能试验,得到纸钵质量、纸钵和转运杯之间直径差与落料成功率的关系,结果表明:纸钵质量水平为高、纸钵和转运杯之间半径差为3mm时,成功率可达94.44%,生产可达3 130穴/h。 展开更多
关键词 育苗 纸钵 自动装盘 穴盘 转运杯
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基于Web of Science秸秆还田研究态势分析 预览
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作者 王麒 曾宪楠 +3 位作者 冯延江 孙羽 宋秋来 来永才 《农机化研究》 北大核心 2020年第5期263-268,共6页
为了解秸秆还田领域的研究进展、热点及趋势,基于Web of Science数据库,采用文献计量学分析方法,分析了1999-2018年秸秆还田领域文章。结果表明:1999-2018年世界范围内秸秆还田文章数量逐年增加,共计33 974篇;中国、美国、印度、巴西和... 为了解秸秆还田领域的研究进展、热点及趋势,基于Web of Science数据库,采用文献计量学分析方法,分析了1999-2018年秸秆还田领域文章。结果表明:1999-2018年世界范围内秸秆还田文章数量逐年增加,共计33 974篇;中国、美国、印度、巴西和西班牙位居世界的前5位,中国文章数量最多;中国科学院、中国农业大学、加拿大农业食品公司、华南理工大学、法国农业科学研究院文章数量分别为前5位;论文主要发表在《BIORESOURCE TECHNOLOGY》《BIORESOURCES》《BIOMASS BIOENERGY》《INDUSTRIAL CROPS AND PRODUCTS》《ENERGY FUELS》;主要研究学科是农学、能源燃料及工程学相关研究学科。 展开更多
关键词 秸秆还田 文献计量 研究态势
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Measurement of the neutron total cross section of carbon at the Back-n white neutron beam of CSNS 预览
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作者 Xing-Yan Liu Yi-Wei Yang +78 位作者 Rong Liu Jie Wen Zhong-Wei Wen Zi-Jie Han Zhi-Zhou Ren Qi An Huai-Yong Bai Jie Bao Ping Cao Qi-Ping Chen Yong-Hao Chen Pin-Jing Cheng Zeng-Qi Cui Rui-Rui Fan Chang-Qing Feng Min-Hao Gu Feng-Qin Guo Chang-Cai Han Guo-Zhu He Yong-Cheng He Yue-Feng He Han-Xiong Huang Wei-Ling Huang Xi-Ru Huang Xiao-Lu Ji Xu-Yang Ji Hao-Yu Jiang Wei Jiang Han-Tao Jing Ling Kang Ming-Tao Kang Bo Li Lun Li Qiang Li Xiao Li Yang Li Shu-Bin Liu Guang-Yuan Luan Ying-Lin Ma Chang-Jun Ning Bin-Bin Qi Jie Ren Xi-Chao Ruan Zhao-Hui Song Hong Sun Xiao-Yang Sun Zhi-Jia Sun Zhi-Xin Tan Hong-Qing Tang Jing-Yu Tang Peng-Cheng Wang Qi Wang Tao-Feng Wang Yan-Feng Wang Zhao-Hui Wang Zheng Wang Qing-Biao Wu Xiao-Guang Wu Xuan Wu Li-Kun Xie Han Yi Li Yu Tao Yu Yong-Ji Yu Guo-Hui Zhang Jing Zhang Lin-Hao Zhang Li-Ying Zhang Qing-Min Zhang Qi-Wei Zhang Xian-Peng Zhang Yu-Liang Zhang Zhi-Yong Zhang Ying-Tan Zhao Liang Zhou Zu-Ying Zhou Dan-Yang Zhu Ke-Jun Zhu Peng Zhu 《核技术:英文版》 SCIE CAS CSCD 2019年第9期73-82,共10页
To verify the performance of the neutron total cross-sectional spectrometer, the neutron total cross section of carbon is initially measured in the energy range of 1 eV to 20 MeV using the time-of-flight method. The m... To verify the performance of the neutron total cross-sectional spectrometer, the neutron total cross section of carbon is initially measured in the energy range of 1 eV to 20 MeV using the time-of-flight method. The measurement is performed at the Back-n white neutron source with a 76-m time-of-flight path using the China Spallation Neutron Source. A multilayer fast fission chamber with 235U and 238U is employed as the neutron detector. The diameter and thickness of the natural graphite sample are 70 mm and 40 mm, respectively. Signal waveforms are collected using a data acquisition system. Off-line data processing was used to obtain the neutron time-of-flight spectra and transmissions. The uncertainty of the counting statistics is generally approximately 3% for each bin in the energy range of 1–20 MeV. It is determined that the results for the neutron total cross section of carbon obtained using ^235U cells are in good agreement with the results obtained using 238U cells within limits of statistical uncertainty. Moreover, the measured total cross sections show good agreement with the broadening evaluated data. 展开更多
关键词 CSNS Back-n WHITE NEUTRON source NTOX NEUTRON total cross section CARBON Multilayer fast FISSION chamber
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Effects of environmental contaminants on fertility and reproductive health
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作者 Yanmin Ma Xin He +5 位作者 Keyan Qi Tong Wang Yongchao Qi Lele Cui Fengbang Wang Maoyong Song 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第3期210-217,共8页
Recent research indicates that the human infertility rate is increasing. Although various reasons have been hypothesized for the growing infertility rate, environmental contaminants are potentially important causal ag... Recent research indicates that the human infertility rate is increasing. Although various reasons have been hypothesized for the growing infertility rate, environmental contaminants are potentially important causal agents associated with this change.Chemical contaminants are widespread throughout our environment and human exposure is virtually unavoidable. The overall contribution of environmental exposure to infertility is unknown, but studies involving occupational exposure, together with results from animal experiments, suggest that environmental contaminants may adversely affect fertility. We reviewed the adverse effects of environmental exposure on fertility and related reproductive outcomes. Environmental contaminants covered in this review include heavy metals, organic solvents, pesticides and endocrine disrupting chemicals. It is hoped that this review will highlight the need for further research in this area. 展开更多
关键词 INFERTILITY REPRODUCTIVE health Environmental CONTAMINANTS Exposure ADVERSE EFFECTS
Measurements of differential and angle-integrated cross sections for the 10B(n,α)^7 Li reaction in the neutron energy range from 1.0 eV to 2.5 MeV
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作者 江浩雨 蒋伟 +85 位作者 白怀勇 崔增琪 张国辉 樊瑞睿 易晗 宁常军 周良 唐靖宇 安琪 鲍杰 鲍煜 曹平 陈昊磊 陈琪萍 陈永浩 陈裕凯 陈朕 封常青 高可庆 顾旻皓 韩长材 韩子杰 贺国珠 何泳成 洪杨 黄翰雄 黄蔚玲 黄锡汝 季筱路 吉旭阳 姜智杰 敬罕涛 康玲 康明涛 李波 李超 李嘉雯 李论 李强 李晓 李样 刘荣 刘树彬 刘星言 栾广源 穆奇丽 齐斌斌 任杰 任智洲 阮锡超 宋朝晖 宋英鹏 孙虹 孙康 孙晓阳 孙志嘉 谭志新 唐洪庆 唐新懿 田斌斌 王丽娇 王鹏程 王琦 王涛峰 王朝辉 文杰 温中伟 吴青彪 吴晓光 吴煊 解立坤 羊奕伟 于莉 余滔 于永积 张林浩 张奇玮 张显鹏 张玉亮 张志永 赵豫斌 周路平 周祖英 朱丹阳 朱科军 朱鹏 《中国物理C:英文版》 SCIE CAS CSCD 2019年第12期47-74,共28页
Differential and angle-integrated cross sections for the 10B(n,α)^7 Li,10B(n,α0)^7 Li and 10B(n,α1)^7 Li^*reactions have been measured at CSNS Back-n white neutron source.Two enriched(90%)10B samples 5.0 cm in diam... Differential and angle-integrated cross sections for the 10B(n,α)^7 Li,10B(n,α0)^7 Li and 10B(n,α1)^7 Li^*reactions have been measured at CSNS Back-n white neutron source.Two enriched(90%)10B samples 5.0 cm in diameter and~85.0μg/cm^2 in thickness each with an aluminum backing were prepared,and back-to-back mounted at the sample holder.The charged particles were detected using the silicon-detector array of the Light-charged Particle Detector Array(LPDA)system.The neutron energy En was determined by TOF(time-of-flight)method,and the valid a events were extracted from the En-Amplitude two-dimensional spectrum.With 15 silicon detectors,the differential cross sections of a-particles were measured from 19.2°to 160.8°.Fitted with the Legendre polynomial series,the(n,a)cross sections were obtained through integration.The absolute cross sections were normalized using the standard cross sections of the 10B(n,α)^7 Li reaction in the 0.3-0.5 MeV neutron energy region.The measurement neutron energy range for the 10B(n,α)^7 Li reaction is 1.0 eV≤En<2.5 Me V(67 energy points),and that for the 10B(n,α0)^7 Li and10B(n,α1)^7 Li^*reactions is 1.0 eV≤En<1.0 MeV(59 energy points).The present results have been analyzed by the resonance reaction mechanism and the level structure of the 11B compound system,and compared with existing measurements and evaluations. 展开更多
关键词 10B(n α)^7Li reaction cross sections LPDA CSNS Back-n white neutron source
中国第一台高性能白光中子源——CSNS反角白光中子源及其应用 预览
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作者 唐靖宇 安琪 +78 位作者 白怀勇 鲍杰 曹平 陈琪萍 陈永浩 程品晶 崔增琪 樊瑞睿 封常青 顾旻皓 郭凤琴 韩长材 韩子杰 贺国珠 何泳成 何越峰 黄翰雄 黄蔚玲 黄锡汝 季筱璐 吉旭阳 江浩雨 蒋伟 敬罕涛 康玲 康明涛 李波 李论 李强 李晓 李洋 李样 刘荣 刘树彬 刘星言 栾广源 马应林 宁常军 齐斌斌 任杰 阮锡超 宋朝晖 孙虹 孙晓阳 孙志嘉 谭志新 唐洪庆 王鹏程 王琦 王涛峰 王艳凤 王朝辉 王征 文杰 温中伟 吴青彪 吴晓光 吴煊 解立坤 羊奕伟 易晗 于莉 余滔 于永积 张国辉 张旌 张林浩 张利英 张清民 张奇玮 张显鹏 张玉亮 张志永 赵映潭 周良 周祖英 朱丹阳 朱科军 朱鹏 《原子能科学技术》 CSCD 北大核心 2019年第10期2012-2022,共11页
基于中国散裂中子源(CSNS)建设的我国第一台高性能白光中子源——反角白光中子源(Back-n)是国际上综合性能最好的白光中子源之一,能区范围覆盖meV~百MeV,飞行时间测量分辨率可在全能区达到1%以内,中子注量率国际领先。自2018年3月建成以... 基于中国散裂中子源(CSNS)建设的我国第一台高性能白光中子源——反角白光中子源(Back-n)是国际上综合性能最好的白光中子源之一,能区范围覆盖meV~百MeV,飞行时间测量分辨率可在全能区达到1%以内,中子注量率国际领先。自2018年3月建成以来,Back-n已开展了一系列的核数据测量实验、探测器标定实验、中子辐射效应实验和中子照相研究,科研产出效率非常高,实验数据质量达到了研究要求,为我国多领域的科学研究和应用研究提供了一个强大的平台。本文对该白光中子源的性能特点、已投入运行和规划中的核数据测量实验谱仪进行了综述,并指出了主要应用方向。 展开更多
关键词 核数据测量 反应截面 白光中子源 实验谱仪
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Human Reproductive Health in Relation to Thyroid Alterations 预览
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作者 Thushani Siriwardhane Karthik Krishna +6 位作者 Qi Song Vinodh Ranganathan Vasanth Jayaraman Tianhao Wang Kang Bei John J. Rajasekaran Hari Krishnamurthy 《健康(英文)》 2019年第8期1095-1133,共39页
Background: Thyroid alterations have adverse effects on other health systems in the body, and reproductive system is one of the most affected organs. Here, we perform a comprehensive analysis on the effects of differe... Background: Thyroid alterations have adverse effects on other health systems in the body, and reproductive system is one of the most affected organs. Here, we perform a comprehensive analysis on the effects of different thyroid abnormalities on sexual and reproductive-related hormones in both men and women in different age groups. Method: A comprehensive analysis was performed on 15,043 subjects within the reproductive age (15 - 49 years). They were tested for thyroid markers (TSH, FT4, anti-TPO, anti-Tg) and 13 reproductive hormones in Vibrant America Clinical Laboratory between March 2016 to July 2018. The alterations of each reproductive hormone in hypothyroidism, hyperthyroidism, anti-TPO+ anti-Tg+ groups were evaluated. Results: A total of 10,626 women was tested. Women with hypothyroidism had elevated cortisol and low SHBG and DHEA-S. Women with hyperthyroidism showed elevated total testosterone and SHBG. Women seropositive for anti-TPO had elevated total testosterone and low cortisol. Women seropositive for anti-Tg had low parathyroid hormones. Similarly, a total of 4417 men was tested. Men with hypothyroidism had low IGF-I. Similarly, men with hyperthyroidism had low DHEA-S, but elevated estradiol, FSH, LH and prolactin. Men seropositive for anti-TPO had elevated SHBG and low progesterone. Men seropositive for anti-Tg had elevated progesterone. The reproductive and related hormone levels of age group 36 - 49 showed the most variations. Conclusion: Our results clearly show that individuals with different thyroid alterations have different effects on reproductive health, especially in the age group 36 - 49. Hence, routine testing and follow-up checkups on reproductive system would be beneficial for individuals in the age group 36 - 49 with thyroid disorders. 展开更多
关键词 REPRODUCTIVE HORMONES HYPOTHYROIDISM HYPERTHYROIDISM THYROID Disease THYROID AUTOANTIBODIES
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Runx2在宫颈癌组织中的表达及其临床意义 预览
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作者 宋聚星 齐文秀 +3 位作者 李小静 锅红芬 宋英 安琪 《肿瘤药学》 CAS 2019年第4期621-625,共5页
目的探讨Runx2基因在宫颈癌组织中的表达及其临床意义。方法采用RT-PCR及Western blotting法检测80例宫颈癌患者的癌组织及相应癌旁组织和22例正常宫颈组织中Runx2基因和蛋白的表达;采用免疫组化法检测Runx2在三组组织中的阳性表达率,... 目的探讨Runx2基因在宫颈癌组织中的表达及其临床意义。方法采用RT-PCR及Western blotting法检测80例宫颈癌患者的癌组织及相应癌旁组织和22例正常宫颈组织中Runx2基因和蛋白的表达;采用免疫组化法检测Runx2在三组组织中的阳性表达率,并分析Runx2与宫颈癌临床分期及分化程度的关系。结果宫颈癌组织中Runx2的阳性率为78.9%(62/80),癌旁组织中为35.0%(28/80),正常宫颈织中未见Runx2的表达。宫颈癌组织中Runx2基因和蛋白的表达均较相应癌旁组织及正常组织显著上调(P<0.05)。低分化、中分化及高分化的宫颈癌组织中Runx2的阳性率分别为89.4%(34/38)、75.0%(18/24)及11.1%(2/18),肿瘤组织分化程度越低,Runx2的阳性率越高。Runx2在Ⅲ、Ⅱ、Ⅰ期肿瘤组织中表达阳性率分别为94.4%(34/36)、57.1%(16/28)、25.0%(4/16),肿瘤组织临床分期越晚,Runx2的阳性率越高。结论宫颈癌组织中Runx2的表达上调,并可能促进了宫颈癌的发生和发展。 展开更多
关键词 宫颈癌 Runx2基因 临床意义
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针灸相关疗法对多囊卵巢综合征患者排卵率及妊娠率影响的网状Meta分析 被引量:1
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作者 宋燕娟 梁凤霞 +9 位作者 吴松 杨会生 陈丽 黄琪 唐宏图 卢威 王华 陈松 张艳佶 张艳琳 《中国针灸》 CAS CSCD 北大核心 2019年第7期792-798,共7页
目的:系统评价针灸相关疗法对多囊卵巢综合征(PCOS)患者排卵率及妊娠率安全性和有效性的影响。方法:计算机检索PubMed、荷兰医学文献数据库(EMbase)、Cochrane图书馆(Cochrane Library)、中国生物医学文献数据库(CBM)、中国期刊全文数据... 目的:系统评价针灸相关疗法对多囊卵巢综合征(PCOS)患者排卵率及妊娠率安全性和有效性的影响。方法:计算机检索PubMed、荷兰医学文献数据库(EMbase)、Cochrane图书馆(Cochrane Library)、中国生物医学文献数据库(CBM)、中国期刊全文数据库(CNKI)、万方(Wanfang)数据库及维普(VIP)数据库,搜集针灸治疗PCOS的随机对照试验(RCT),检索时限均为从建库至2018年1月8日。由2位研究者独立筛选文献、提取数据和评价纳入研究的偏倚风险后,采用Stata13.0和WinBUGS 1.4.3软件进行数据分析。结果:共纳入39个RCT,包括4605例PCOS患者,涉及针灸相关疗法及其综合疗法14种,均为短期疗效观察。网状Meta分析结果显示:在排卵率方面,针药联合疗法疗效优于西药,排名前6位的治疗措施分别为穴位埋线+药物联合疗法(93.3%)、艾灸+中药(91.4%)、艾灸(74.5%)、针药联合疗法(65.7%)、针灸+耳穴(61.9%)、针刺+耳穴(49.6%);在妊娠率方面,针药联合疗法疗效优于西药,排名前6位的治疗措施分别为针刺+耳穴(91.5%)、艾灸+中药(86.9%)、针灸+耳穴(81.1%)、穴位埋线+药物联合疗法(69.4%)、针药联合疗法(66.1%)、安慰剂+西药(58.7%)。结论:在针灸相关疗法中,联合疗法优于单一疗法,且安全性优于西药疗法,各类联合疗法也互有优劣。由于现有研究的局限性,本研究的结论还需大量设计合理、高质量且方法得当的RCT予以证实。 展开更多
关键词 多囊卵巢综合征 排卵率 妊娠率 针灸相关疗法 网状Meta分析
Di (2-ethylhexyl) phthalate Disorders Lipid Metabolism via TYK2/STAT1 and Autophagy in Rats 预览
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作者 ZHANG Yue Zhu ZHANG Zhao Ming +10 位作者 ZHOU Li Ting ZHU Jian ZHANG Xiao Han QI Wen DING Shuang XU Qi HAN Xu ZHAO Ya Ming SONG Xin Yue ZHAO Tian Yang YE Lin 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第6期406-418,共13页
Objective Previous studies have indicated that the plasticizer di(2-ethylhexyl) phthalate(DEHP) affects lipid accumulation;however, its underlying mechanism remains unclear. We aim to clarify the effect of DEHP on lip... Objective Previous studies have indicated that the plasticizer di(2-ethylhexyl) phthalate(DEHP) affects lipid accumulation;however, its underlying mechanism remains unclear. We aim to clarify the effect of DEHP on lipid metabolism and the role of TYK2/STAT1 and autophagy. Methods In total, 160 Wistar rats were exposed to DEHP [0, 5, 50, 500 mg/(kg·d)] for 8 weeks. Lipid levels, as well as mRNA and protein levels of TYK2, STAT1, PPARγ, AOX, FAS, LPL, and LC3 were detected. Results The results indicate that DEHP exposure may lead to increased weight gain and altered serum lipids. We observed that DEHP exposure affected liver parenchyma and increased the volume or number of fat cells. In adipose tissue, decreased TYK2 and STAT1 promoted the expression of PPARγ and FAS. The mRNA and protein expression of LC3 in 50 and 500 mg/(kg·d) groups was increased significantly. In the liver, TYK2 and STAT1 increased compensatorily;however, the expression of FAS and AOX increased, while LPL expression decreased. Joint exposure to both a high-fat diet and DEHP led to complete disorder of lipid metabolism. Conclusion It is suggested that DEHP induces lipid metabolism disorder by regulating TYK2/STAT1. Autophagy may play a potential role in this process as well. High-fat diet, in combination with DEHP exposure, may jointly have an effect on lipid metabolism disorder. 展开更多
关键词 DEHP TYK2 STAT1 HIGH-FAT diet AUTOPHAGY Lipid metabolism
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基于低摩擦和SMA的新型自复位支撑力学性能与设计方法 预览
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作者 顾琦 宋固全 +2 位作者 胡淑军 郭琪 姜俊 《南昌大学学报:工科版》 CAS 2019年第3期265-270,303共7页
结合形状记忆合金、扩孔型螺栓连接和低摩擦原理,提出一种强耗能、自复位、低残余变形的新型自复位支撑。采用校正的有限元方法对合理设计的新型自复位支撑模型进行分析,以得到其滞回曲线、应力、骨架曲线和累积耗能能力等,由此明确其... 结合形状记忆合金、扩孔型螺栓连接和低摩擦原理,提出一种强耗能、自复位、低残余变形的新型自复位支撑。采用校正的有限元方法对合理设计的新型自复位支撑模型进行分析,以得到其滞回曲线、应力、骨架曲线和累积耗能能力等,由此明确其力学性能。通过理论分析推导新型自复位支撑的力学模型和设计方法,且与有限元分析结果对比,验证力学模型的正确性。研究表明:新型自复位支撑呈现出饱满的滞回曲线,且残余变形较小,具有良好的自复位能力。 展开更多
关键词 自复位支撑 形状记忆合金 低摩擦 长圆孔 力学性能
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油菜品种宁油26的选育与栽培要点 预览
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作者 付三雄 戚存扣 +10 位作者 周晓婴 张洁夫 陈锋 陈松 龙卫华 浦惠明 高建芹 张维 胡茂龙 彭琦 王晓东 《种业导刊》 2019年第4期19-21,共3页
宁油26是从甘蓝型油菜组合“中双9号×扬6026”中杂交选育而成的常规油菜新品种,属甘蓝型油菜早、中熟品种类型。从选育过程、产量表现、品质性状、抗性表现、特征特性、农艺性状、经济性状入手,总结了油菜品种宁油26的栽培技术、... 宁油26是从甘蓝型油菜组合“中双9号×扬6026”中杂交选育而成的常规油菜新品种,属甘蓝型油菜早、中熟品种类型。从选育过程、产量表现、品质性状、抗性表现、特征特性、农艺性状、经济性状入手,总结了油菜品种宁油26的栽培技术、种子选育技术要点。 展开更多
关键词 甘蓝型油菜 栽培技术 杂交选育 宁油26
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Whole Genome Analyses of Chinese Population and De Novo Assembly of A Northern Han Genome
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作者 Zhenglin Du Liang Ma +27 位作者 Hongzhu Qu Wei Chen Bing Zhang Xi Lu Weibo Zhai Xin Sheng Yongqiao Sun Wenjie Li Meng Lei Qiuhui Qi Na Yuan Shuo Shi Jingyao Zeng Jinyue Wang Yadong Yang Qi Liu Yaqiang Hong Lili Dong Zhewen Zhang Dong Zou Yanqing Wang Shuhui Song Fan Liu Xiangdong Fang Hua Chen Xin Liu Jingfa Xiao Changqing Zeng 《基因组蛋白质组与生物信息学报:英文版》 CAS CSCD 2019年第3期229-247,共19页
To unravel the genetic mechanisms of disease and physiological traits,it requires comprehensive sequencing analysis of large sample size in Chinese populations.Here,we report the primary results of the Chinese Academy... To unravel the genetic mechanisms of disease and physiological traits,it requires comprehensive sequencing analysis of large sample size in Chinese populations.Here,we report the primary results of the Chinese Academy of Sciences Precision Medicine Initiative(CASPMI)project launched by the Chinese Academy of Sciences,including the de novo assembly of a northern Han reference genome(NH1.0)and whole genome analyses of 597 healthy people coming from most areas in China.Given the two existing reference genomes for Han Chinese(YH and HX1)were both from the south,we constructed NH1.0,a new reference genome from a northern individual,by combining the sequencing strategies of PacBio,10×Genomics,and Bionano mapping.Using this integrated approach,we obtained an N50 scaffold size of 46.63 Mb for the NH1.0 genome and performed a comparative genome analysis of NH1.0 with YH and HX1.In order to generate a genomic variation map of Chinese populations,we performed the whole-genome sequencing of 597 participants and identified 24.85 million(M)single nucleotide variants(SNVs),3.85 M small indels,and 106,382 structural variations.In the association analysis with collected phenotypes,we found that the T allele of rs1549293 in KAT8 significantly correlated with the waist circumference in northern Han males.Moreover,significant genetic diversity in MTHFR,TCN2,FADS1,and FADS2,which associate with circulating folate,vitamin B12,or lipid metabolism,was observed between northerners and southerners.Especially,for the homocysteine-increasing allele of rs1801133(MTHFR 677T),we hypothesize that there exists a “comfort”zone for a high frequency of 677T between latitudes of 35–45 degree North.Taken together,our results provide a high-quality northern Han reference genome and novel population-specific data sets of genetic variants for use in the personalized and precision medicine. 展开更多
关键词 De novo assembly Reference GENOME Variation map PHENOTYPE association Large POPULATION
Differentially expressed long noncoding RNAs and regulatory mechanism of LINC02407 in human gastric adenocarcinoma 预览
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作者 Li-Li Zhou Yan Jiao +9 位作者 Hong-Mei Chen Li-Hua Kang Qi Yang Jing Li Meng Guan Ge Zhu Fei-Qi Liu Shuang Wang Xue Bai Yan-Qiu Song 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第39期5973-5990,共18页
BACKGROUND Long noncoding RNAs(lncRNAs)have been identified to play important roles in the development and progression of various tumors,including gastric cancer(GC).However,the molecular role of lncRNAs in GC progres... BACKGROUND Long noncoding RNAs(lncRNAs)have been identified to play important roles in the development and progression of various tumors,including gastric cancer(GC).However,the molecular role of lncRNAs in GC progression remains unclear.AIM To investigate the differential expression of lncRNAs in human GC and elucidate the function and regulatory mechanism of LINC02407.METHODS The Cancer Genome Atlas database was used to investigate the involvement of lncRNAs in GC.Quantitative real-time polymerase chain reaction was used to estimate the relative expression level of LINC02407 in GC tissues and cells.Functional experiments including CCK8 assay,apoptosis assay,wound healing assay,and transwell assay were used to investigate the effect of LINC02407 on GC cells.Some microRNAs were predicted and verified via bioinformatics analysis and the luciferase reporter system.Predictive analysis and Western blot assay were used to analyze the expression of related proteins.RESULTS Many differentially expressed lncRNAs were identified in GC,and some of them including LINC02407 can affect the survival.LINC02407 was upregulated in tumor tissues compared with adjacent tissues.HGC-27 cells showed the highest LINC02407 expression and HaCaT cells exhibited the lowest expression.Different experiment groups were constructed using LINC02407 overexpressing plasmids and related siRNAs.The results of functional experiments showed that LINC02407 can promote the proliferation,migration,and invasion of GC cells but inhibit apoptosis.Luciferase reporter assay showed that hsa-miR-6845-5p and hsa-miR-4455 was downstream regulated by LINC02407.Western blot analysis showed that adhesion G protein-coupled receptor D1(ADGRD1)was regulated by the LINC02407-miR-6845-5p/miR-4455-ADGRD1 pathways.CONCLUSION LINC02407 plays a role in GC through the LINC02407-miR-6845-5p/miR-4455-ADGRD1 pathways,and thus,it may be an important oncogene and has potential value in GC diagnosis and treatment. 展开更多
关键词 Gastric cancer LONG noncoding RNAS LINC02407 ADHESION G proteincoupled receptor D1 MicroRNA-6845-5p MicroRNA-4455
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基于RNA-Seq筛选APEC及其PhoP/Q缺失株感染雏鸡肠道免疫相关基因
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作者 徐柳柳 祁克宗 +3 位作者 涂健 宋祥军 薛媚 王琦 《基因组学与应用生物学》 CAS CSCD 北大核心 2019年第1期159-164,共6页
本研究以禽致病性大肠杆菌(APEC)及其PhoP/Q缺失株感染雏鸡小肠为模型,以分析其免疫相关基因的表达变化为目的,采用转录组测序(RNA-Seq)技术对感染APEC及其PhoP/Q缺失株的雏鸡小肠样本RNA进行测序,分析免疫相关基因的表达变化,结果为野... 本研究以禽致病性大肠杆菌(APEC)及其PhoP/Q缺失株感染雏鸡小肠为模型,以分析其免疫相关基因的表达变化为目的,采用转录组测序(RNA-Seq)技术对感染APEC及其PhoP/Q缺失株的雏鸡小肠样本RNA进行测序,分析免疫相关基因的表达变化,结果为野生株攻毒组与对照组相比、野生株攻毒组与缺失株攻毒组相比、缺失株攻毒组与对照组相比,分别筛选出131、105、172个差异表达基因(fold change≥2, FDR≤0.05),GO功能分类结果显示分别有87、99、159个基因得到注释,这些基因主要富集到氧化还原过程、脂蛋白转运、血管内皮细胞迁移、免疫反应、凋亡过程负调控、肝素结合、铁离子结合、CCR趋化因子受体结合等功能,得出APEC及其PhoP/Q缺失株感染雏鸡后引起机体肠道免疫相关基因变化的结论,根据GO功能注释筛选出PTPRC、LCP1、YFV等免疫相关基因,为深入研究雏鸡肠道免疫提供依据。 展开更多
关键词 禽致病性大肠杆菌 PhoP/PhoQ二元调控系统 雏鸡小肠 转录组测序 免疫相关基因
Surgical management of newborns with combined tracheoesophageal fistula, esophageal atresia, and duodenal obstruction
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作者 Zhu-Ping Cao Qi-Feng Li +5 位作者 Shi-Qi Liu Jian-Hua Niu Jing-Ru Zhao Ya-Jun Chen Da-Yong Wang Xiao-Song Li 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第6期726-730,共5页
To the Editor: The incidence of esophageal atresia (EA) along with tracheoesophageal fistula (TEF) is approximately 1/3000 in newborns.[1] A large population study revealed that 46% of patients with TEF/EA had at leas... To the Editor: The incidence of esophageal atresia (EA) along with tracheoesophageal fistula (TEF) is approximately 1/3000 in newborns.[1] A large population study revealed that 46% of patients with TEF/EA had at least one of the other vertebral, anal, cardiac, tracheal, esophageal, renal, and limb (VACTERL) malformations.[2] Duodenal obstruction has an incidence of 1/7000 live births and can be caused by duodenal atresia (DA) or annular pancreas (AP).[3] AP is an uncommon congenital anomaly, and duodenal obstruction most commonly presents in the infancy or early childhood of patients.[4] The incidence of DA combined with EA varies between 3% and 6%,[5] and DA combined with EA is often associated with significant morbidity and mortality.[6] 展开更多
关键词 the EDITOR INCIDENCE approximately
儿科抗病毒药物应用现状研究
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作者 宋岐 李月阳 +1 位作者 宋燕青 张文锐 《长春中医药大学学报》 2019年第3期500-502,共3页
目的分析我院儿科抗病毒药物的临床使用情况,促进合理用药。方法调取我院儿科2018年4月1日-9月30日住院期间使用过抗病毒药物注射剂的患儿,共计12 937例,涉及5 127例患儿;随机抽取20例,共计260例病例进行抗病毒注射剂使用点评,进行合理... 目的分析我院儿科抗病毒药物的临床使用情况,促进合理用药。方法调取我院儿科2018年4月1日-9月30日住院期间使用过抗病毒药物注射剂的患儿,共计12 937例,涉及5 127例患儿;随机抽取20例,共计260例病例进行抗病毒注射剂使用点评,进行合理性分析。结果在统计的人群中,幼儿期和婴儿期合计3 188人,占62.18%;抗病毒药使用西药以注射用单磷酸阿糖腺苷为主,占41.70%;中药以注射用炎琥宁为主,占24.51%,共计有74例病例存在使用2种及以上的抗病毒药物,占28.46%,且较多存在中西药联合抗病毒治疗情况。结论临床抗病毒药物注射剂的使用不合理情况较多,药师需加强处方点评,规范抗病毒药物的合理使用。 展开更多
关键词 儿童 抗病毒药物 临床应用 合理用药
肺腺癌表皮生长因子受体基因突变与临床病理特征的关系
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作者 朱森林 齐宇 +4 位作者 张春敭 刘帅帅 侯志超 宋亚男 赵松 《中华实用诊断与治疗杂志》 2019年第10期1002-1004,共3页
目的探讨肺腺癌表皮生长因子受体(epidermal growth factor receptor, EGFR)基因突变与其临床病理特征的关系。方法 278例肺腺癌患者,采用荧光PCR法检测手术切除标本中的EGFR基因,分析肺腺癌EGFR基因突变率与临床病理特征的关系;采用多... 目的探讨肺腺癌表皮生长因子受体(epidermal growth factor receptor, EGFR)基因突变与其临床病理特征的关系。方法 278例肺腺癌患者,采用荧光PCR法检测手术切除标本中的EGFR基因,分析肺腺癌EGFR基因突变率与临床病理特征的关系;采用多因素logistic回归分析肺腺癌EGFR基因突变的影响因素;分析浸润性腺癌不同组织亚型EGFR基因突变情况。结果 278例肺腺癌中EGFR基因突变为61.9%,女性(69.4%)、无吸烟史(67.3%)、肿瘤直径≤2.4 cm(66.9%)、浸润性腺癌(65.9%)患者EGFR基因突变率明显高于男性(52.1%)、有吸烟史(42.6%)、肿瘤直径>2.4 cm(54.5%)、浸润性腺癌变异型(10.0%)患者(P<0.05),低分化腺癌患者EGFR基因突变率(44.2%)低于中分化(67.7%)和高分化腺癌(80.0%)(P<0.05);中分化腺癌(OR=1.534,95%CI:1.056~8.274,P=0.032)、浸润性腺癌(OR=6.588,95%CI:5.879~32.316,P=0.001)是肺腺癌患者发生EGFR基因突变的独立影响因素;浸润性腺癌不同组织亚型中腺泡型(71.3%)、附壁生长型(68.8%)、乳头型(61.1%)、微乳头型(57.1%)、实体型(29.2%)患者EGFR基因突变率依次降低(P<0.05)。结论 EGFR基因突变与性别、有无吸烟史、肿瘤直径、分化程度、组织学亚型有关;中分化腺癌、浸润性腺癌是影响肺腺癌患者发生EGFR基因突变的独立因素,腺泡型为主肺腺癌的EGFR突变率最高。 展开更多
关键词 肺腺癌 表皮生长因子受体 基因突变
铜催化β,γ-不饱和腙与二硫/硒化物的硫/硒胺基化反应:合成硫/硒化吡唑啉类化合物
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作者 任培星 齐林 +6 位作者 方卓越 吴天舒 高雅蒙 沈松 宋金燕 王力竞 李玮 《有机化学》 SCIE CAS CSCD 北大核心 2019年第6期1776-1786,共11页
发展了一类β,γ-不饱和腙与二硫/硒化物在铜催化下进行的自由基历程的硫/硒胺基化反应,合成了一系列的硫/硒化吡唑啉类化合物.该反应操作简便且反应条件温和.
关键词 铜催化 β γ-不饱和腙 硫/硒胺基化 吡唑啉
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新型冠状病毒肺炎防控与诊疗专栏