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Effects of neural stem cell transplantation on the motor function of rats with contusion spinal cord injuries:a meta-analysis 预览
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作者 Kai Qian Tuo-Ye Xu +7 位作者 Xi Wang Tao Ma Kai-Xin Zhang Kun Yang Teng-Da Qian Jing Shi Li-Xin Li Zheng Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期748-758,共11页
Objective:To judge the efficacies of neural stem cell(NSC)transplantation on functional recovery following contusion spinal cord injuries(SCIs).Data sources:Studies in which NSCs were transplanted into a clinically re... Objective:To judge the efficacies of neural stem cell(NSC)transplantation on functional recovery following contusion spinal cord injuries(SCIs).Data sources:Studies in which NSCs were transplanted into a clinically relevant,standardized rat model of contusion SCI were identified by searching the PubMed,Embase and Cochrane databases,and the extracted data were analyzed by Stata 14.0.Data selection:Inclusion criteria were that NSCs were used in in vivo animal studies to treat contusion SCIs and that behavioral assessment of locomotor functional recovery was performed using the Basso,Beattie,and Bresnahan lo-comotor rating scale.Exclusion criteria included a follow-up of less than 4 weeks and the lack of control groups.Outcome measures:The restoration of motor function was assessed by the Basso,Beattie,and Bresnahan locomotor rating scale.Results:We identified 1756 non-duplicated papers by searching the aforementioned electronic databases,and 30 full-text articles met the inclusion criteria.A total of 37 studies reported in the 30 articles were included in the meta-analysis.The meta-analysis results showed that transplanted NSCs could improve the motor function recovery of rats following contusion SCIs,to a moderate extent(pooled standardized mean difference(SMD)=0.73;95%confidence interval(CI):0.47–1.00;P<0.001).NSCs obtained from different donor species(rat:SMD=0.74;95%CI:0.36–1.13;human:SMD=0.78;95%CI:0.31–1.25),at different donor ages(fetal:SMD=0.67;95%CI:0.43–0.92;adult:SMD=0.86;95%CI:0.50–1.22)and from different origins(brain-derived:SMD=0.59;95%CI:0.27–0.91;spinal cord-derived:SMD=0.51;95%CI:0.22–0.79)had similar efficacies on improved functional recovery;however,adult induced pluripotent stem cell-derived NSCs showed no significant efficacies.Furthermore,the use of higher doses of transplanted NSCs or the administration of immunosuppressive agents did not promote better locomotor function recovery(SMD=0.45;95%CI:0.21–0.70).However,shorter periods between the contusion induction and the NSC tr 展开更多
关键词 Basso Beattie and Bresnahan locomotor rating scale CELL TRANSPLANTATION META-ANALYSIS motor functional recovery NEURAL regeneration NEURAL stem CELL NEURAL stem CELL TRANSPLANTATION rat model SPINAL CONTUSION SPINAL cord injury
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Different protein expression patterns in rat spinal nerves during wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling 预览
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作者 Shuai Wei Xue-Zhen Liang +12 位作者 Qian Hu Wei-Shan Wang Wen-Jing Xu Xiao-Qing Cheng Jiang Peng Quan-Yi Guo Shu-Yun Liu Wen Jiang Xiao Ding Gong-Hai Han Ping Liu Chen-Hui Shi Yu Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期315-323,共9页
Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a label... Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the differences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between s 展开更多
关键词 gene ontology Kyoto ENCYCLOPEDIA of Genes and Genomes ISOBARIC tags for RELATIVE and absolute quantitation motor NERVE PROTEOMICS sensory NERVE spinal NERVE Wallerian degeneration
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喘可治注射液对甲型H1N1流感病毒FM1株致小鼠肺炎的影响
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作者 姚荣妹 毛鑫 +5 位作者 曲天歌 鮑岩岩 孙静 时瀚 时宇静 崔晓兰 《中国实验方剂学杂志》 CAS CSCD 北大核心 2019年第12期43-49,共7页
目的:评价喘可治注射液对甲型H1N1流感病毒FM1株致小鼠肺炎模型的药效作用。方法:将ICR小鼠随机分为正常组、模型组、达菲组(27.5 mg·kg^-1·d^-1)、喘可治注射液组(1.5 m L·kg-1·d^-1)。除正常组外,其余各组采用甲... 目的:评价喘可治注射液对甲型H1N1流感病毒FM1株致小鼠肺炎模型的药效作用。方法:将ICR小鼠随机分为正常组、模型组、达菲组(27.5 mg·kg^-1·d^-1)、喘可治注射液组(1.5 m L·kg-1·d^-1)。除正常组外,其余各组采用甲型H1N1流感病毒FM1株滴鼻感染ICR小鼠造成病毒性肺炎模型,分别进行死亡保护实验、治疗性给药实验和预防性给药实验。死亡保护实验中每组20只小鼠,以2倍半数致死量(LD50)流感病毒液滴鼻感染,感染后当天开始给药,每天1次,连续4 d,观察感染后14 d内动物的死亡情况,并计算死亡率、死亡保护率;治疗性给药实验中每组10只小鼠,以0.8倍LD50流感病毒液滴鼻感染,感染后当天开始给药,每天1次,连续4 d,于实验第5天处死小鼠,采用酶联免疫吸附测定(ELISA)检测肺组织中白细胞介素-8(IL-8)含量及脑组织内发热介质前列腺素E2(PGE2),精氨酸加压素(AVP)的含量,实时荧光定量聚合酶链式反应(Real-time PCR)检测肺组织内相对病毒载量;预防性给药实验中每组10只小鼠,分组当天开始给药,每天1次,连续5 d,末次给药1 h后,除正常组外,其余各组以0.8倍LD50流感病毒滴鼻感染ICR小鼠,感染后第4天称重后处死小鼠,计算各组肺指数、脾指数、胸腺指数及肺指数抑制率,Real-time PCR检测肺组织内相对病毒载量。结果:与正常组比较,模型组小鼠IL-8,PGE2含量,肺指数及肺组织内流感病毒株病毒载量均显著升高(P<0.01);与模型组比较,治疗性给予喘可治注射液可明显降低感染后小鼠的死亡率、肺组织内IL-8含量、脑组织内PGE2含量及肺组织中流感病毒病毒载量(P<0.05,P<0.01);预防性给予喘可治注射液可显著提高感染小鼠的胸腺指数(P<0.01),明显降低感染小鼠肺指数及肺组织中流感病毒病毒载量(P<0.05);两种给药方式均可明显减轻支气管、血管周围及肺泡间质等组织炎性细胞浸润,减少管腔内红细胞渗出。结� 展开更多
关键词 喘可治注射液 甲型H1N1流感病毒 病毒性肺炎 药效作用
Focus on Cancer Pain at Home: A Prospective Study of Quality of Life and Its Influencing Factors on Patients 预览
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作者 Aijun Ji Ruchun Shi +1 位作者 Jing Han Chen Yu 《癌症治疗(英文)》 2019年第10期815-828,共14页
Background and Aims: The treatment of patients with advanced cancer pain is mainly concentrated in the outpatient department, and most of the time in their family, these patients are easy to be ignored, To study the q... Background and Aims: The treatment of patients with advanced cancer pain is mainly concentrated in the outpatient department, and most of the time in their family, these patients are easy to be ignored, To study the quality of life and its influencing factors of cancer pain patients at home is of great significance to improve the quality of life of patients. Meanwhile, it provides theoretical and practical basis for medical personnel to develop and implement individualized comprehensive intervention programs. Patients and Methods: According to the inclusion and exclusion criteria, 200 patients with cancer pain at home are selected to treat, and their quality of life conditions are observed before treatment, 1 week after treatment and 1 month after treatment, and their influencing factors are analyzed. Results: The patients’ scores of body function, emotional function, cognitive function and social function exist significant difference before and after treatment (p scores of role function and the overall evaluation scores before and after treatment, two stages after treatment exist significant difference (p < 0.01), the symptoms scores of fatigue, pain, diarrhea, nausea and vomiting are significant differences before and after treatment (p appetite loss score before a month and a week after treatment and treatment exists significant difference (p the scores of constipation symptom before treatment and a month after treatment exist significant difference (p < 0.01), only gender on cognitive function before treatment has significant difference (p . One week after treatment, tumor staging and metastasis have impact on overall health evaluation, role function, cognitive function and emotional function (p The location of metastasis and the type of pain affect the role function and emotional function respectively (p . One month after treatment, age, metastasis, metastasis site and pain type have influence on cognitive function, emotional function, overall health evaluation and role function respectively (p The overall 展开更多
关键词 Cancer PAIN PROSPECTIVE Study Quality of Life Influence FACTOR HOME
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Comparison of Rapid Freezing and Vitrification for Human Sperm Cryopreservation Using Trehalose as a Cryoprotective Agent 预览
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作者 Huanhuan Hu Rui Liu +7 位作者 Xiaowei Shi Guojie Ji Jing Zhang Han Zhang Pengbo Wang Zhibin Sun Guoao Xiao Mingwen Li 《妇产科期刊(英文)》 2019年第10期1407-1418,共12页
Rapid freezing and vitrification are becoming popular for human sperm cryopreservation;however, it remains unclear which method is better. The aims of the present study were to determine the optimal trehalose concentr... Rapid freezing and vitrification are becoming popular for human sperm cryopreservation;however, it remains unclear which method is better. The aims of the present study were to determine the optimal trehalose concentration and to compare the cryoprotective effects of rapid freezing and vitrification. The results showed that: 1) The optimal trehalose concentration was 0.25 mol/L;2) The post-thaw recovery rates of total and progressive sperm motilities after rapid freezing (38.6% ± 3.0% and 41.1% ± 5.0%) were significantly higher (P 0.05) than that after vitrification (26.1% ± 3.1% and 27.2% ± 1.3%) when 0.5 mL straws were used;3) However, the recovery rates of total and progressive motilities after rapid freezing in 0.5 mL straw (26.7% ± 9.6% and 26.8% ± 8.7%) were significantly lower (P 0.05) than that after vitrification in a novel straw-in-straw system (43.1% ± 4.2% and 41.8% ± 15.5%);and 4) The post-thaw sperm nuclear DNA damage level after rapid freezing in 0.5 mL straw (8.7% ± 2.8%) was not significantly different from that of sperm after vitrification in the straw-in-straw system (9.2% ± 2.5%). It was concluded that rapid freezing is superior to vitrification when using 0.5 mL straws;however, vitrification is superior to rapid freezing when using the straw-in-straw systems. 展开更多
关键词 Human SPERM RAPID FREEZING VITRIFICATION TREHALOSE
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Human Comfort Instrument Design Based on Embedded 预览
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作者 Shucheng Chen Jing Shi +2 位作者 Xiaobo Li Ming Cui Lianwei Su 《地球科学和环境保护期刊(英文)》 2019年第6期115-124,共10页
The traditional human comfort meter has the following defects: the interface is not uniform;the operation is cumbersome and complicated;the interface is unfriendly, and the stability and adaptability are poor. This pa... The traditional human comfort meter has the following defects: the interface is not uniform;the operation is cumbersome and complicated;the interface is unfriendly, and the stability and adaptability are poor. This paper presents a design scheme for human comfort instrument based on embedded system, using S3C2440 embedded development board and the sensors to collect the real-time temperature, relative humidity and wind speed data and to process the collecting data;then obtaining the human body comfort value according to the basic algorithm of human body comfort instrument;giving the human comfort conclusion according to the diastolic index range of human comfort, and showing the temperature and humidity, wind speed, comfort value and conclusion through writing the Qt graphical user interface program. At the same time, the human comfort instrument has the data storage function. The human comfort instrument is high in integration, strong in real time, high in sensitivity, stable and reliable, and it meets the development goals of the intelligent meteorological service, and meets the demand of the meteorological service that is closer to life, and it has broad development prospect. 展开更多
关键词 HUMAN COMFORT Instrument EMBEDDED S3C2440 QT
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复方土贝母制剂对裸鼠乳腺癌MCF-7移植瘤生长的抑制作用及对E-cad、Vimentin mRNA表达的影响 预览
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作者 董晶 凌仕良 +2 位作者 史国军 王邦才 施航 《中国中医药科技》 CAS 2019年第5期664-666,共3页
目的:研究复方土贝母制剂对MCF-7移植瘤抑制作用及对E-cad(E-钙粘蛋白)、Vimentin(波形蛋白) mRNA表达的影响。方法:40只雌性Balb/c裸鼠皮下接种造模后随机分为荷瘤对照组和复方土贝母制剂低(0. 015 g/kg)、中(0. 030 g/kg)、高(0. 060 ... 目的:研究复方土贝母制剂对MCF-7移植瘤抑制作用及对E-cad(E-钙粘蛋白)、Vimentin(波形蛋白) mRNA表达的影响。方法:40只雌性Balb/c裸鼠皮下接种造模后随机分为荷瘤对照组和复方土贝母制剂低(0. 015 g/kg)、中(0. 030 g/kg)、高(0. 060 g/kg)剂量给药组。各给药组予不同浓度(0. 5、1. 0、2. 0 g/m L)复方土贝母制剂连续灌胃30 d,灌胃容积均为0. 6 m L/20 g,对照组予生理盐水灌胃。所有小鼠第30天处死,测量各组瘤重、计算抑瘤率,实时荧光定量(PCR)法检测E-cad、Vimentin mRNA表达水平。结果:复方土贝母制剂低、中、高剂量组抑瘤率分别为13. 11%、38. 69%、42. 29%,中、高剂量给药组瘤重与对照组比较差异有统计学意义(P <0. 05);各剂量给药组瘤组织E-cad、Vimentin mRNA表达与对照组比较差异均有统计学意义(P <0. 05)。结论:复方土贝母制剂可抑制乳腺癌生长,其机制可能与上调E-cad表达、下调Vimentin表达有关。 展开更多
关键词 乳腺癌 复方土贝母制剂 E-钙粘蛋白 波形蛋白 肿瘤生长
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Clinical deep remission and related factors in a large cohort of patients with rheumatoid arthritis
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作者 Jia-Jia Liu Ru Li +14 位作者 Yu-Zhou Gan Rui-Jun Zhang Jing Li Yue-Ming Cai Jin-Xia Zhao Hua Liao Jing Xu Lian-Jie Shi Ji Li Sheng-Guang Li Xiao-Lin Sun Jing He Xu Liu Hua Ye Zhan-Guo Li 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第9期1009-1014,共6页
Background:Clinical remission is the treatment target in rheumatoid arthritis (RA).This study aimed to investigate clinical remission and related factors in a large cohort of patients with RA.Methods:This study compos... Background:Clinical remission is the treatment target in rheumatoid arthritis (RA).This study aimed to investigate clinical remission and related factors in a large cohort of patients with RA.Methods:This study composed of 342 patients with RA.Data were collected by face-to-face interview of 1049 patients with RA who visited the Department of Rheumatology of three teaching hospitals from September 2015 to May 2016.The patients with RA were clinically assessed by rheumatologists and a four-page questionnaire was completed on site.Subsequently,patients fulfilled remission criteria were further analyzed.The practicability of different definitions of remission of RA was rated by a panel of rheumatologists.Sustained intensive disease modifying anti-rheumatic drug (DMARD) treatment was defined as a combination treatment with two or more DMARDs for at least 6 months.Results:In this cohort of 342 patients with RA,the proportions of patients achieving remission were 38.0%,29.5%,24.9%,21.1%,19.0%,18.1%,and 17.0%,based on criteria of disease activity score in 28 joints (DAS28) using CRP (DAS28-CRP),DAS28 using ESR (DAS28-ESR),routine assessment of patient index data 3 (RAPID-3),Boolean,simplified disease activity index (SDAI),clinical disease activity index,and the newly described clinical deep remission (CliDR),respectively.Boolean and CliDR are the best in practicability scored by rheumatologists (7.5 and 8.0,respectively).Compared with the non-sustained intensive group,sustained intensive treatment with DMARDs yielded higher remission rates of 25.6%,23.8%,and 21.3% in patients with RA based on Boolean (χ^2=3.937,P=0.047),SDAI (χ^2=4.666,P=0.031),and CliDR criteria (χ^2=4.297,P=0.038).The most commonly prescribed conventional synthesized DMARDs (csDMARDs) in patients with RA was leflunomide,followed by methotrexate,and hydroxychloroquine.Compared with the non-remission group,patients achieving remission had a longer median duration of DMARDs (45.0 [22.8–72.3] months,Z=-2.295,P=0.022).Conclusions:The findings in this 展开更多
关键词 RHEUMATOID arthritis REMISSION Sustained Intensive Disease MODIFYING anti-rheumatic drug
Clinical characteristic and fecal microbiota responses to probiotic or antidepressant in patients with diarrhea-predominant irritable bowel syndrome with depression comorbidity: a pilot study
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作者 Lu Zhang Yi-Xuan Liu +9 位作者 Zhe Wang Xiao-Qi Wang Jing-Jing Zhang Rong-Huan Jiang Xiang-Qun Wang Shi-Wei Zhu Kun Wang Zuo-Jing Liu Huai-Qiu Zhu Li-Ping Duan 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第3期346-351,共6页
To the Editor: Irritable bowel syndrome (IBS) is a prevalent functional gastrointestinal disorder that presents as abdominal pain with altered bowel habits. The pathophysiologic mechanism of IBS is not well understood... To the Editor: Irritable bowel syndrome (IBS) is a prevalent functional gastrointestinal disorder that presents as abdominal pain with altered bowel habits. The pathophysiologic mechanism of IBS is not well understood, although many hypotheses have been proposed, including visceral hypersensitivity, gastrointestinal dysmotility, lowgrade inflammation of the intestinal mucosa, and dysfunction of the brain-gut interaction. Dysfunction of the braingut interaction is thought to be involved in IBS because a considerable proportion of patients with IBS have some form of psychologic comorbidity, such as depression or anxiety. In addition, mental and psychologic problems increase the risk for IBS and the symptom severity of IBS. Recently, changes in the gut microbiota have also been suggested to contribute to both IBS and depression. Our previous study demonstrated significant altered gut microbiota profiles in patients with diarrhea-predominant IBS (IBS-D) and depression. 展开更多
关键词 FECAL microbiota RESPONSES DEPRESSION COMORBIDITY diarrhea-predominant IRRITABLE bowel syndrome
慢性应激致抑郁小鼠不同组织C型钠尿肽表达的变化 预览
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作者 任敬一 付媛媛 +4 位作者 荆潇橦 苏玉娇 史晓瑞 杜玉茹 史海水 《中国医药导报》 CAS 2019年第22期26-29,共4页
目的探讨慢性应激致抑郁小鼠不同组织和血清中C型钠尿肽(CNP)表达的影响。方法选取20只雄性ICR小鼠,并按照随机数字表法将其分为对照组和应激组,每组10只。采用慢性轻度应激(CMS)方法建立抑郁小鼠模型,经动物行为学检测后取材,采用酶联... 目的探讨慢性应激致抑郁小鼠不同组织和血清中C型钠尿肽(CNP)表达的影响。方法选取20只雄性ICR小鼠,并按照随机数字表法将其分为对照组和应激组,每组10只。采用慢性轻度应激(CMS)方法建立抑郁小鼠模型,经动物行为学检测后取材,采用酶联免疫吸附测定(ELISA)法对小鼠海马、肝、肾、心和血清中CNP进行定量分析。结果行为学测试结果显示,与对照组比较,应激组小鼠糖水偏爱值明显减小,强迫游泳测试、悬尾测试的不动潜伏期明显缩短,强迫游泳的不动时间明显延长,差异均有统计学意义(P<0.05);两组小鼠总摄水量比较,差异无统计学意义(P>0.05)。与对照组比较,应激组小鼠心、肾和血清中CNP水平明显升高,差异均有统计学意义(均P<0.05)。结论慢性应激可引起心、肾和血清中CNP水平显著升高,提示外周组织的CNP表达变化可能与应激致抑郁的发生发展有关。 展开更多
关键词 抑郁症 慢性应激 钠尿肽 C型钠尿肽
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Characteristics of β-oxidative and reductive metabolism on the acyl side chain of cinnamic acid and its analogues in rats
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作者 Kai-jing Zhao Yang Chen +11 位作者 Shi-jin Hong Yi-ting Yang Jiong Xu Han-yu Yang Liang Zhu Ming Liu Qiu-shi Xie Xian-ge Tang Ting-ting Yang Ya-qian Zhou Li Liu Xiao-dong Liu 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第8期1106-1118,共13页
Cinnamic acid and its analogues (pyragrel and ozagrel) undergo chain-shortened (β-oxidative) and reductive metabolism on acyl side chain. In this study, we characterized the β-oxidative and reductive metabolism on a... Cinnamic acid and its analogues (pyragrel and ozagrel) undergo chain-shortened (β-oxidative) and reductive metabolism on acyl side chain. In this study, we characterized the β-oxidative and reductive metabolism on acyl side chain of cinnamic acid and its analogues using primary rat hepatocytes, hepatic mitochondrial, and microsomal systems. A compartmental model including parent compounds and metabolites was developed to characterize in vivo β-oxidative and reductive metabolism following an intravenous dose of parent compounds to rats. The ?tted total in vivo clearance values were further compared with the in vitro values predicted by the well-stirred model. We showed that hepatic microsomal CYP450s did not catalyze β-oxidative or reductive metabolism of the three compounds. Similar to β-oxidation of fatty acids,β-oxidative metabolism on their acyl side chain occurred mainly in mitochondria, which was highly dependent on ATP, CoA and NAD+. Fatty acids and NADH inhibited the β-oxidative metabolism. Reductive metabolism occurred in both mitochondria and microsomes. Reduction in mitochondria was ATP-, CoA-, and NAD(P)H-dependent and reversible, which was suppressed by enoyl reductase inhibitor triclosan. Reduction in microsomes was ATP-, CoA-, and NADPH-dependent but little affected by triclosan. Both plasma concentrations of β-oxidative metabolites and reductive metabolites were successfully ?tted using the compartmental model. The estimated total in vivo clearance values were consistent with those predicted from hepatocytes and organelles, implicating signi?cance of in vitro kinetics. These ?ndings demonstrate the roles of hepatic mitochondria and microsomes in β-oxidative and reductive metabolism on acyl side chain of cinnamic acid and its analogues along with their metabolic characteristics. 展开更多
关键词 ANTIPLATELET DRUG cinnamic acid pyragrel OZAGREL DRUG METABOLISM hepatocytes mitochondria MICROSOMES pharmacokinetics in vitro-in vivo correlation
物联网与虚拟现实融合的智能火灾营救机制
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作者 闫丰亭 史志才 +4 位作者 苏前敏 董婧 陈珏 石蕴玉 汤显 《系统仿真学报》 CAS CSCD 北大核心 2019年第11期2306-2322,共17页
虚拟现实、物联网及人工智能的融合,可为火灾救援决策提供智能WebVR交互。然而需要解决数据轻量化、基于物联网实时数据传输,以及火灾情景数据在网页端可视化的问题,需要构建有效的机制、算法。提出一种构建物联网与虚拟现实融合的智能... 虚拟现实、物联网及人工智能的融合,可为火灾救援决策提供智能WebVR交互。然而需要解决数据轻量化、基于物联网实时数据传输,以及火灾情景数据在网页端可视化的问题,需要构建有效的机制、算法。提出一种构建物联网与虚拟现实融合的智能火灾营救(IoTandVirtualReality based Intelligent Fire Rescue,IVIFR)机制,提出了虚拟智能火灾救援(Virtual Intelligent Fire Rescue,VIFR)算法,构建了智慧火灾救援原型系统。将VIFR算法与贪心算法、随机算法针对营救被困人员的成功率,进行了比较。实验证明VIFR算法的营救成功率均高于其他3种算法。 展开更多
关键词 物联网 网络虚拟现实 火灾营救 IVIFR机制 VIFR算法
Attenuation of periostin in retinal Müller glia by TNF-αand IFN-γ
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作者 Ying-Qian Peng Man-Jing Cao +8 位作者 Shigeo YoShida Lu-Si Zhang Hui-Lan Zeng Jing-Ling Zou Yoshiyuki Kobayashi Takahito Nakama Jing-Ming Shi Song-Bai Jia Ye-Di Zhou 《国际眼科杂志:英文版》 SCIE CAS 2019年第2期212-218,共7页
AIM:To investigate the regulation and mechanisms of periostin expression in retinal Müller glia, and to explore the relevance to retinal neovascularization. METHODS:The oxygen-induced retinopathy(OIR) mouse model... AIM:To investigate the regulation and mechanisms of periostin expression in retinal Müller glia, and to explore the relevance to retinal neovascularization. METHODS:The oxygen-induced retinopathy(OIR) mouse model and the human Moorfield/Institute of Ophthalmology-Müller 1(MIO-M1) cell line were used in the study. Immunofluorescence staining was used to determine the distribution and expression of periostin and a Müller glial cell marker glutamine synthetase(GS). Cytokines TNF-α and IFN-γ were added to stimulate the MIO-M1 cells. ShRNA was used to knockdown periostin expression in MIO-M1 cells. Quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR) was conducted to assess the mRNA expression of periostin. RESULTS:Immunofluorescence staining showed that periostin was expressed by MIO-M1 Müller glia. GS-positive Müller glia and periostin increased in OIR retinas, and were partially overlaid. The stimulation of TNF-α and IFN-γ reduced the mRNA expression of periostin significantly and dose-dependently in MIO-M1 cells. Knockdown of periostin reduced mRNA expression of vascular endothelial growth factor A(VEGFA) in MIO-M1 cells, while VEGFA expression was not changed in periostin knock-out OIR retinas. CONCLUSION:Müller glia could be one of the main sources of periostin in the retina, and might contribute to the pathogenesis of retinal neovascularization. Proinflammatory cytokines TNF-α and IFN-γ attenuate the periostin expression in retinal Müller glia, which provides a potential and novel method in treating retinal neovascular diseases. 展开更多
关键词 TNF-Α IFN-Γ PERIOSTIN Müller GLIA RETINAL neovascularization
干燥方式对NaY(WO4)2:Dy3+,Eu3+荧光粉粒度及上转换发光性能的影响
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作者 史忠祥 王晶 +4 位作者 卢杨 关昕 时军 戴丽静 刘冰倩 《过程工程学报》 CAS CSCD 北大核心 2019年第2期323-328,共6页
采用水热法合成了Dy3+和Eu3+共掺杂的NaY(WO4)2上转换荧光粉,用XRD, SEM和荧光光谱(PL)等方法对不同干燥方式所得样品的晶体结构、微观形貌、晶粒尺寸及上转换发光性能进行了分析。结果表明,合成样品均为四方白钨矿结构的NaY(WO4)2,空... 采用水热法合成了Dy3+和Eu3+共掺杂的NaY(WO4)2上转换荧光粉,用XRD, SEM和荧光光谱(PL)等方法对不同干燥方式所得样品的晶体结构、微观形貌、晶粒尺寸及上转换发光性能进行了分析。结果表明,合成样品均为四方白钨矿结构的NaY(WO4)2,空间群为I41/a,掺入Dy3+和Eu3+未改变基质晶格;常规干燥、喷雾干燥、冷冻干燥及真空干燥后荧光粉的尺寸分别为817.91, 486.04,388.74和349.82nm,真空干燥的样品分散性最好。在793nm近红外光激发下,冷冻干燥样品的上转换发光性能最佳,与干燥过程中粉体团聚程度减弱及表面层缺陷减少有关。576 nm处的黄光发射峰来自Dy3+的4F9/2→6H13/2跃迁,595 nm处的橙光、616和655 nm处的红光发射峰分别归属于Eu3+的5D0→7F1, 5D0→7F2和5D0→7F3跃迁。荧光粉的CIE色坐标均位于红光区,表现出良好的红光发射特性,在发光二极管和彩色显示等光电领域具有潜在应用价值。 展开更多
关键词 水热法 干燥方式 荧光粉 粒度 上转换发光
不同剂量米库氯铵在不同年龄儿童气管异物取出术麻醉中的应用 预览
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作者 史静 石磊 +3 位作者 王俊霞 赵海涛 包文娟 容俊芳 《河北医科大学学报》 CAS 2019年第4期466-471,共6页
目的探讨不同剂量米库氯铵在不同年龄儿童气管异物取出术麻醉中的应用。方法选择拟行气管异物取出术儿童100例,依据其年龄和米库氯铵的诱导剂量随机分为4组各25例。Ⅰa、Ⅰb组(幼儿组,1~3岁)分别于麻醉诱导时静脉注射米库氯铵0.15mg/kg... 目的探讨不同剂量米库氯铵在不同年龄儿童气管异物取出术麻醉中的应用。方法选择拟行气管异物取出术儿童100例,依据其年龄和米库氯铵的诱导剂量随机分为4组各25例。Ⅰa、Ⅰb组(幼儿组,1~3岁)分别于麻醉诱导时静脉注射米库氯铵0.15mg/kg、0.20mg/kg;Ⅱa、Ⅱb组(年长儿组,>3~5岁)分别于麻醉诱导时静脉注射米库氯铵0.15mg/kg,0.20mg/kg。术中观察并记录肌松时效指标,包括起效时间,临床作用时间,RI、TOFr75和TOFr90的时间。观察并记录置镜前后MAP、HR、SpO2的变化,置镜满意率和不良反应的发生情况。结果不同剂量米库氯铵起效时间比较:与Ⅰa、Ⅱa组比较,Ⅰb、Ⅱb组ThD75、ThD90和ThDmax明显缩短,差异均有统计学意义(P<0.05)。不同年龄米库氯铵起效时间比较:与Ⅰa、Ⅱa组比较,Ⅱa、Ⅱb组ThDmax明显延长,差异有统计学意义(P<0.05)。各组临床作用时间,RI、TOFr75、TOFr90的时间差异均无统计学意义(P>0.05)。4组置镜满意率分别为96.0%、100.0%、88.0%和96.0%,其差异无统计学意义(P>0.05)。MAP、HR、SpO2在组间、时点间、组间·时点间交互作用差异均无统计学意义(P>0.05)。4组颈前及胸前皮肤潮红发生率差异无统计学意义(P>0.05),4组均未发生血压下降和支气管痉挛。结论在应用同等剂量米库氯铵进行麻醉诱导时,幼儿的起效时间显著短于年长儿,但对肌松的恢复无影响;0.15mg/kg米库氯铵即可为行气管异物取出术儿童提供满意的肌松效果。 展开更多
关键词 气管 异物 米库氯铵 儿童
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Normal values of shear wave velocity in liver tissue of healthy children measured using the latest acoustic radiation force impulse technology 预览
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作者 Pei-Xuan Sun Yu-Yang Tong +3 位作者 Jing Shi Huan Zhang Shi-Jian Liu Jun Du 《世界临床病例杂志》 2019年第21期3463-3473,共11页
BACKGROUND Several studies have demonstrated the feasibility and effectiveness of using ultrasound elastography to assess liver tissue stiffness.Virtual touch imaging quantification(VTIQ)based on acoustic radiation fo... BACKGROUND Several studies have demonstrated the feasibility and effectiveness of using ultrasound elastography to assess liver tissue stiffness.Virtual touch imaging quantification(VTIQ)based on acoustic radiation force impulse imaging has been developed as a latest and noninvasive method for assessing liver stiffness in children.AIM To determine the standard value in healthy children,and to identify possible factors that might influence the VTIQ measurement.METHODS With the ethical approval,202 children between 1 month and 15 years old were included in this study.None of them had any liver or systematic diseases.All children had a normal ultrasound scan and normal body mass index(BMI)range.The subjects were divided into four age and BMI groups.The effects of gender,age,liver lobe,measurement depth,and BMI on liver elasticity were investigated.RESULTS A significant correlation was found between age and shear wave velocity(SWV)value.At measurement depths of 1.5 cm and 2.0 cm in the left lobe,there were significant differences among the age groups.SWV values were significantly negatively correlated with the measurement depth.Gender,liver lobe,and BMI showed no significant effect on the SWV values.Age and BMI may influence the quality of the elastogram.CONCLUSION VTIQ is a noninvasive technique that is feasible to measure liver stiffness in children.The afore-mentioned velocity value obtained utilizing VTIQ method could be used as reference value for normal liver stiffness in children. 展开更多
关键词 Acoustic radiation force impulse Virtual touch imaging quantification Liver stiffness Shear wave velocity
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桩蛋白基因克隆及其在肝癌细胞Hep G2中的作用 预览
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作者 孙达权 石静 +2 位作者 石松 薛殿婷 徐莹莹 《新医学》 2019年第9期671-675,共5页
目的克隆桩蛋白基因(PXN)的蛋白编码区并构建PXN基因真核表达载体,建立PXN稳转肝癌细胞株,分析PXN基因对肝癌细胞的作用.方法Trizol法提取肝癌细胞总RNA并逆转录成cDNA,PCR法扩增PXN基因蛋白编码区并构建真核表达质粒pEBFP-PXN.脂质体... 目的克隆桩蛋白基因(PXN)的蛋白编码区并构建PXN基因真核表达载体,建立PXN稳转肝癌细胞株,分析PXN基因对肝癌细胞的作用.方法Trizol法提取肝癌细胞总RNA并逆转录成cDNA,PCR法扩增PXN基因蛋白编码区并构建真核表达质粒pEBFP-PXN.脂质体法转染肝癌细胞HepG2,G418筛选培养获得桩蛋白高表达稳转肝癌细胞株.实时无标记细胞分析检测细胞生长速率,细胞创伤-愈合实验分析细胞迁移速率.蛋白免疫印迹法检测细胞内蛋白表达变化.结果成功克隆PXN基因并构建真核表达载体pEBFP-PXN.获得桩蛋白高表达单克隆稳转肝癌细胞株,胞内显示蓝色荧光,蛋白免疫印迹法显示稳转株内表达外源融合蛋白BFP-PXN.桩蛋白表达不影响HepG2肝癌细胞的生长速率但可以抑制其细胞迁移,稳转细胞上皮标志蛋白E-Cadherin表达升高、间质标志物N-Cadherin和Vimentin表达下降.结论PXN基因不影响HepG2肝癌细胞生长增殖速率但可以抑制其细胞迁移,这可能与其可以影响肝癌细胞上皮-间质转化标志蛋白表达有关. 展开更多
关键词 肝癌细胞 桩蛋白基因 真核表达 细胞迁移
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“四维一体”模式下大学生创业教育专业教育的有效融合 预览 被引量:2
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作者 史晶 石茶 《高教学刊》 2019年第3期21-23,共3页
创业教育作为一种新的人才培养方式,正逐渐作为高校的教育教学改革重点被不断完善和深化。创业教育与专业教育进行融合,才能有效培养出具有创新意识和创业能力的高素质人才。文章从剖析创业教育与专业教育深度融合的现实意义着手,从思... 创业教育作为一种新的人才培养方式,正逐渐作为高校的教育教学改革重点被不断完善和深化。创业教育与专业教育进行融合,才能有效培养出具有创新意识和创业能力的高素质人才。文章从剖析创业教育与专业教育深度融合的现实意义着手,从思维路径、理论基础、互动平台、实践支点四个方面进行融合教育探索,力图打造“四维一体”模式下两者融合新的教育模式。 展开更多
关键词 创业教育 专业教育 四维一体 融合
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动眼神经麻痹33例临床分析 预览
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作者 井艳丽 石亚军 +4 位作者 周林甫 李仓霞 李敏昌 安一菲 史锋 《脑与神经疾病杂志》 2019年第8期474-479,共6页
目的探讨动眼神经麻痹(ONP)患者的临床表现及病因。方法分析2012年9月至2017年12月陕西解放军第三医院神经内科住院治疗的33例ONP患者的病例资料。结果33例ONP患者中单眼发病30例陕西(90.9%),双眼发病3例(9.1%)。病因分析结果显示:糖尿... 目的探讨动眼神经麻痹(ONP)患者的临床表现及病因。方法分析2012年9月至2017年12月陕西解放军第三医院神经内科住院治疗的33例ONP患者的病例资料。结果33例ONP患者中单眼发病30例陕西(90.9%),双眼发病3例(9.1%)。病因分析结果显示:糖尿病9例(27.3%),脑干梗死6例(18.2%),脑神经炎3例(9.0%),病毒性脑炎2例(6.1%),痛性眼肌麻痹2例(6.1%),重症肌无力2例(6.1%),颅内动脉瘤2例(6.1%),先天性动眼神经麻痹1例(3.0%),多发性硬化3例(9.1%),病因不明3例(9.1%)。治疗及随访结果:22例(66.7%)患者临床症状完全恢复,包括糖尿病性动眼神经麻痹9例(27.3%),脑干梗死5例(15.2%),脑神经炎3例(9.1%),重症肌无力2例(6.1%),病毒性脑炎2例(6.1%),后交通动脉瘤1例(3.0%)。结论临床上根据动眼神经麻痹患者发病年龄、起病方式、临床症状及相关辅助检查大多数可明确诊断,早期、合理的综合治疗可使大部分患者临床症状改善。 展开更多
关键词 动眼神经麻痹 临床表现 病因 诊断
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Precision Higgs physics at the CEPC
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作者 安芬芬 白羽 +70 位作者 陈春晖 陈新 陈振兴 Joao Guimaraes da Costa 崔振崴 方亚泉 付成栋 高俊 高艳彦 高原宁 葛韶锋 顾嘉荫 郭方毅 郭军 韩涛 韩爽 何红建 何显柯 何小刚 胡继峰 徐士杰 金山 荆茂强 Susmita Jyotishmati Ryuta Kiuchi 郭家铭 赖培筑 李博扬 李聪乔 李刚 李海峰 李亮 李数 李通 李强 梁浩 梁志均 廖立波 刘波 刘建北 刘涛 刘真 娄辛丑 马连良 Bruce Mellado 莫欣 Mila Pandurovic 钱剑明 钱卓妮 Nikolaos Rompotis 阮曼奇 Alex Schuy 单连友 史静远 史欣 苏淑芳 王大勇 王锦 王连涛 王贻芳 魏彧骞 许悦 杨海军 杨迎 姚为民 于丹 张凯栗 张照茹 赵明锐 赵祥虎 周宁 《中国物理C:英文版》 SCIE CAS CSCD 2019年第4期5-44,共40页
The discovery of the Higgs boson with its mass around 125 GeV by the ATLAS and CMS Collaborations marked the beginning of a new era in high energy physics.The Higgs boson will be the subject of extensive studies of th... The discovery of the Higgs boson with its mass around 125 GeV by the ATLAS and CMS Collaborations marked the beginning of a new era in high energy physics.The Higgs boson will be the subject of extensive studies of the ongoing LHC program.At the same time,lepton collider based Higgs factories have been proposed as a possible next step beyond the LHC,with its main goal to precisely measure the properties of the Higgs boson and probe potential new physics associated with the Higgs boson.The Circular Electron Positron Collider(CEPC)is one of such proposed Higgs factories.The CEPC is an e^+e^- circular collider proposed by and to be hosted in China.Located in a tunnel of approximately 100 km in circumference,it will operate at a center-of-mass energy of 240 GeV as the Higgs factory.In this paper,we present the first estimates on the precision of the Higgs boson property measurements achievable at the CEPC and discuss implications of these measurements. 展开更多
关键词 CEPC HIGGS BOS on HIGGS BOSON properties HIGGS BOSON COUPLINGS HIGGS FACTORY effective field theory EFT
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