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富血小板血浆复合骨骼肌干细胞可促进骨修复 预览

Bone repair using skeletal muscle stem cells combined with platelet-rich plasma
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摘要 背景:富血小板血浆含有的血小板颗粒富含多种活性因子,将其作为促进干细胞分化的细胞因子进行组织工程骨修复具有一定的优势。目的:探讨富血小板血浆复合骨骼肌干细胞促进兔肱骨近端骨缺损修复的效果。方法:制备兔富血小板血浆,提取兔比目鱼肌组织中的骨骼肌干细胞。将28只新西兰白兔随机分为4组,自然愈合组、骨骼肌干细胞组,富血小板血浆组、复合材料组,每组7只,均制备肱骨近端骨缺损模型,后3组造模后骨缺损处分别植入同种异体骨骼肌干细胞、自体富血小板血浆、富血小板血浆复合骨骼肌干细胞。术后进行CT检查及组织学观察骨愈合情况。结果与结论:①术后4周起复合材料组影像学上成骨速率明显高于其他3组(P <0.05);骨骼肌干细胞组、富血小板血浆组成骨速率无明显差异(P> 0.05),均明显高于自然愈合组(P <0.05);②复合材料组骨缺损处组织学评分最高,骨骼肌干细胞组和富血小板血浆组组织学评分无明显差异,自然愈合组组织学评分最低;③结果表明,富血小板血浆和骨骼肌干细胞都具有促进骨修复的作用,而二者结合能更好地修复骨缺损。 BACKGROUND:Platelet-rich plasma contains a variety of active factors,which has certain advantages as a cytokine that promotes stem cell differentiation for bone tissue engineering.OBJECTIVE:To investigate the effect of skeletal muscle stem cells combined with platelet-rich plasma to promote the repair of rabbit bone.METHODS:(1)Rabbit platelet-rich plasma was prepared and skeletal muscle stem cells from rabbit soleus muscle were isolated.Twenty-eight New Zealand white rabbits were randomly divided into four groups(n=7/group),in which the proximal humeral bone defect model was prepared in each rabbit,followed by implantation of nothing(natural healing group),allogeneic skeletal muscle stem cells,autologous platelet-rich plasma or platelet-rich plasma combined with skeletal muscle stem cells,respectively.CT and histological examinations were performed to detect bone healing postoperatively.RESULTS AND CONCLUSION:In the postoperative 4-week CT film,new bone formation was faster in the combination group than the other three groups(P<0.05).No significant difference in the bone formation rate was observed in the skeletal muscle stem cell and platelet-rich plasma groups(P>0.05),but the bone formation rate in these two groups was significantly better than that in the natural healing group(P<0.05).(2)Histological score of the one defect specimens was highest in the combination group and lowest in the natural healing group,and there was no significant difference between the skeletal muscle stem cell and platelet-rich plasma groups.These results reveal that both platelet-rich plasma and skeletal muscle stem cells could promote bone repair,and their combination could achieve better outcomes in bone repair.
作者 殷诺 薛锋 肖海军 丁亮 袁君杰 潘明芒 喻都 鞠金勇 朱龙章 Yin Nuo;Xue Feng;Xiao Haijun;Ding Liang;Yuan Junjie;Pan Mingmang;Yu Du;Ju Jinyong;Zhu Longzhang(Shanghai Fengxian District Central Hospital(South Campus,Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University),Shanghai 201499,China)
出处 《中国组织工程研究》 CAS 北大核心 2019年第17期2678-2683,共6页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 上海市医学重点专科建设项目(ZK2015B06) 上海市奉贤区科委项目(奉卫科2010-01) 上海市健康医学院种子基金项目(HMSF-17-21-025).
关键词 富血小板血浆 骨骼肌干细胞 肱骨近端骨缺损 诱导成骨 骨修复 骨折愈合 成骨速度 platelet-rich plasma skeletal muscle stem cells proximal humeral defect osteogenic induction bone repair fracture healing bone formation rate
作者简介 殷诺,男,1982年生,黑龙江省哈尔滨市人,汉族,2008年上海交通大学医学院毕业,硕士,主治医师,主要从事组织工程骨修复的相关研究;通讯作者:朱龙章,硕士,副主任医师,上海市奉贤区中心医院(上海交通大学附属第六人民医院南院),上海市201499.
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