目的:分析NSCLC EGFR突变与吸烟等因素的交互作用,及其与miR-221表达的相关性,为肺癌分子机制研究提供线索和依据。方法:采用高分辨熔解曲线法(high resolution melting,HRM)进行EGFR突变检测,以EGFR突变阳性为病例组,野生型为对照组,logistic回归分析EGFR突变与吸烟等因素在肺癌中的交互作用,Spearman分析EGFR突变与miR-221表达的相关性。结果:吸烟者相对于不吸烟者,EGFR突变差异具有统计学意义(P=0.034),不吸烟者EGFR突变率较高。性别、年龄、饮酒、组织学类型、肿瘤直径、TNM分期、分化程度、肿瘤家族史以及心脑血管疾病史与EGFR突变无统计学关系(P>0.05);NSCLC EGFR突变与吸烟、饮酒、肿瘤家族史以及心脑血管疾病史对肺癌的发生不存在相乘交互作用;miR-221表达在NSCLC组织明显高于非癌肺组织,差异有统计学意义(P=0.017);EGFR突变与miR-221表达无相关性(r=-0.034,P=0.858)。结论:NSCLC EGFR突变与吸烟、饮酒、肿瘤家族史及心脑血管疾病史均不存在相乘交互作用,miR-221表达在NSCLC组织中高于非癌病人肺组织,与EGFR突变无相关性。
Objective:To detect the interaction of epidermal growth factor receptor(EGFR) mutations with smoking and other factors in non-small cell lung cancer(NSCLC).And find out the correlation between miR-221 expression and EGFR mutations to provide clue and basis for studying the potential molecular mechanism of lung cancer.Methods:We took the EGFR mutation as the case group and the wild type for the control group to analyse the interaction of EGFR mutation with smoking & other factors by logistic regression.EGFR mutant was detected by high resolution melting(HRM).The correlation between miR-221 expression and EGFR gene mutations was done by Spearman correlation analysis.Results:Significant difference of EGFR gene mutation was observed between smoking and nonsmoking(P=0.034).No significant difference was observed between EGFR gene mutation and other factors and clinic pathologic parameters,including gender,age,drinking,histology,tumor size,p-TNM stage,degrees of pathological differentiation,family history of cancer and history of cardiovascular disease(P>0.05).There were no multiplicative interactions between EGFR mutation and smoking,drinking,family history of cancer,history of cardiovascular disease in lung cancer.The expression level of miR-221 was significantly higher in NSCLC tissues than in lung benign lesion tissues( P = 0. 017) and no correlation between the expression of miR-221 and EGFR mutation was found( r =-0. 034,P = 0. 858). Conclusion:No multiplicative interactions were observed between EGFR mutation and smoking,drinking,family history of cancer and history of cardiovascular disease in NSCLC. miR-221 expression was up-regulated in NSCLC tumor tissues compared to the control untumor lung tissues. No correlation was observed between the expression of miR-221 and EGFR mutation in NSCLC.