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沙门氏菌裂解性噬菌体的分离鉴定及其生物学特性 预览 被引量:13

Isolation and Biological Characteristics of Lytic Salmonella Bacteriophage
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摘要 从家禽屠宰场污水中,以肠炎沙门氏菌ATCC13076和禽沙门氏菌CVCC2184为宿主菌,分离到两株裂解性沙门氏菌噬菌体,分别命名为v B_Sen M-PA13076(简称PA13076)和v B_Sen M-PC2184(简称PC2184)。同时对这两株噬菌体进行了噬菌斑、噬菌谱系(含耐药菌)、形态学(透射电镜)、遗传物质、酸碱及热稳定性、最佳感染复数、一步生长曲线等生物学特性研究。结果表明:两株噬菌体的裂解空斑均透亮清晰;除对本身宿主菌产生强裂解作用,还裂解其他沙门氏菌,为宽噬菌谱,其中PA13076能强裂解一株耐氨苄青霉素ATCC13076转化菌株(A+13076),PC2184则能裂解另一株耐氨苄青霉素CVCC2184转化菌株(A+2184);电镜观察这两株噬菌体均为肌尾病毒科;遗传物质为ds DNA;p H值和温度耐受能力较好;PA13076、PC2184最佳感染复数分别为0.01、10,潜伏期分别为20、30 min,爆发期分别为70、60 min,平均爆发量分别为21、23。 Two lytic Salmonella bacteriophages,namely v B_Sen M-PA13076(PA13076) and v B_Sen M-PC2184(PC2184) that can inactivate ATCC13076 or CVCC2184 were isolated from the sewage samples of poultry slaughtering plants.Their biological properties were identifi ed by plaque,host range(antibiotic-resistant bacteria),transmission electron microscopy(TEM),genetic material,p H and thermostability,optimal multiplicity of infection(MOI) and one-step growth experiment.Thei r plaq ues on host strains were clear and bright,and also caused bacteriolysis to other strains of Salmonella.PA13076 could strongly cause bacteriolysis to an ampicillin-resistant strain SE A+13076,while PC2184 only lyse another strain A+2184 slightly.The newly isolated bacte riophages were the members of family Myoviridae according to their ultrastructure under TEM.Their genomes were ds DNA and revealed excellent tolerance to p H and temperature.The MOI of PA13076(PC2184) was 0.01(10),and the latent period was 20 min(30 min),the burst period was 70 min(60 min) and the average burst size was 21(23) phages per cell.
作者 包红朵 张鹏禹 周艳 张莉莉 张辉 陶明煊 王冉 BAO Hongduo;ZHANG Pengyu;ZHOU Yan;ZHANG Lili;ZHANG Hui;TAO Mingxuan;WANG Ran;Jiangsu Key Laboratory of Food Quality and Safety-State Key Laboratory Cultivation Base of MOST,Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality,Ministry of Agriculture,Institute of Food Safety and Inspection,Jiangsu Academy of Agricultural Sciences;Ginling College,Nanjing Normal University
出处 《食品科学》 CSCD 北大核心 2015年第5期131-136,共6页 Food Science
基金 江苏省自然科学基金项目(BK2012788) 江苏省农业科技自主创新资金探索性项目(CX(12)5040) 国家自然科学基金青年科学基金项目(31402234)
关键词 肠炎沙门氏菌 裂解性噬菌体 生物学特性 耐药性 Salmonella enteritidis lytic phage biological characteristic multi-antibiotic resistance
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参考文献14

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