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表面具有非交联结构糖蛋白识别位点的分子印迹聚合物微球的可控制备 被引量:1

Controlled preparation of molecularly imprinted polymer microspheres with surface uncrosslinked glycoprotein binding cavities
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摘要 将原子转移自由基沉淀聚合技术、表面锚定糖蛋白策略及表面引发的可控自由基聚合方法相结合,发展了一种简便高效地制备表面具有(由亲水性聚合物刷形成的)非交联结构糖蛋白(卵清蛋白(OVA))识别位点的分子印迹聚合物(MIP)微球的新方法.对所得具有不同非交联印迹壳层厚度的MIP微球的形貌、化学结构、表面亲水性及模板吸附性能进行了系统研究.结果表明,该方法可高效制备在水溶液中对OVA具有优异识别性能的MIPs.随着MIP微球表面亲水性聚合物刷的引入,其表面亲水性与水相分散稳定性明显提高;同时亲水性聚合物刷的长度亦对MIPs的模板吸附性能有显著影响:只有当亲水性聚合物刷长度与OVA粒径加上微球表面修饰的苯硼酸基的总长度相近时, MIP微球对OVA的吸附容量与专一性吸附方能达到最优;此外,该MIP还具有良好的OVA选择性. Molecularly imprinted polymers(MIPs)are synthetic receptors with tailor-made recognition sites for target molecules.Their high affinity and selectivity,excellent stability,easy preparation,and low cost make them promising substitutes to biological receptors(e.g.,antibody and enzyme)in many applications where molecular recognition is important.Despite significant progress made in the imprinting of small templates,the imprinting of biomacromolecules(e.g.,proteins)remains a big challenge because of their large sizes,complexed structures,and conformational variability.These inherent characteristics of biomacromolecules lead to many significant problems for the resulting macromolecularly imprinted polymers(mMIPs)such as laborious removal of large templates and their slow access to the binding sites.Recent years have witnessed much efforts being devoted to the development of mMIPs due to their great potential in proteome analysis,clinical diagnostics,and biomedicine.So far,some useful strategies have been developed for the imprinting of proteins,mainly including the bulk polymerization method,epitope imprinting strategy,and surface imprinting approach.Among them,the surface imprinting approach has been most widely used because it can readily lead to mMIPs with higher efficiency for removing large templates and more rapid template binding kinetics.Nevertheless,the presently developed mMIPs normally have crosslinked template binding sites,whose rigid structures might have negative influence on the removal of large templates and template binding kinetics.In this sense,the development of mMIPs with more flexible biomacromolecular binding cavities(e.g.,uncrosslinked ones)should be useful for solving the above problems.To our knowledge,however,only rather limited numbers of publications relating to mMIPs with uncrosslinked binding sites have been disclosed,which are all based on the use of self-assembled monolayer strategy.The development of versatile new approaches for preparing m MIPs with uncrosslinked binding sites and g
作者 黄丽洁 郑从光 张会旗 Lijie Huang;Congguang Zheng;Huiqi Zhang(State Key Laboratory of Medicinal Chemical Biology,Key Laboratory of Functional Polymer Materials of Ministry of Education,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),and College of Chemistry,Nankai University,Tianjin 300071,China)
出处 《科学通报》 EI CAS CSCD 北大核心 2019年第13期1407-1417,共11页 Chinese Science Bulletin
基金 国家自然科学基金(21574070,21774063) 天津市自然科学基金重点项目(16JCZDJC36800)资助.
关键词 分子印迹聚合物 糖蛋白 表面锚定 可控表面印迹 亲水性聚合物刷 未交联的印迹位点 molecularly imprinted polymers glycoprotein surface immobilization controlled surface imprinting hydrophilic polymer brushes uncrosslinked binding sites
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