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五味子醇甲和五味子甲素对四氯化碳所致小鼠急性肝损伤的保护作用及机制

Protective effects of schisandrin and deoxyschisandrin on acute liver injury induced by carbon tetrachloride in mice and its mechanism
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摘要 目的观察五味子醇甲、五味子甲素对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用及其机制。方法按照体重将雄性小鼠随机分为5组:正常组(0. 5%羧甲基纤维素钠)、模型组(0. 5%羧甲基纤维素钠)、对照组(双环醇300mg·kg-1·d-1)、五味子醇甲组(五味子醇甲400 mg·kg-1·d-1)和五味子甲素组(五味子甲素400 mg·kg-1·d-1),每组10只。2次/天,连续灌胃给药7d。末次给药1 h后,除正常组外,其余各组均腹腔注射0. 3%的CCl4花生油溶液,复制小鼠急性肝损伤模型。收集小鼠血清和肝组织。以自动生化分析仪检测各组小鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)水平,试剂盒法测定肝组织中丙二醛(MDA)和超氧化物歧化酶(SOD)水平。结果正常组、模型组、对照组、五味子醇甲和五味子甲素组的ALT水平分别为(30. 90±3. 14),(3986. 90±78. 63),(387. 00±24. 39),(1914. 70±89. 35)和(2142. 10±98. 35)U·L-1;这5组的AST水平分别为(191. 50±18. 02),(2337. 70±80. 34),(978. 10±95. 65),(1525. 60±96. 91)和(1405. 30±92. 31) U·L-1;这5组的肝组织MDA水平分别为(3. 08±0. 18),(8. 67±0. 28),(5. 13±0. 22),(6. 63±0. 28)和(6. 18±0. 34)μmol·g-1;这5组的SOD水平分别为(162. 42±4. 03),(102. 86±3. 55),(148. 78±7. 57),(132. 83±6. 42)和(138. 21±4. 94) U·mg-1。模型组与正常组相比或者对照组、五味子醇甲、五味子甲素组与模型组相比,上述4个指标差异均有统计学意义(均P <0. 01)。结论五味子醇甲、五味子甲素对CCl4所致小鼠急性肝损伤具有一定保护作用,其机制与抗氧化有关。 Objective To observe the protective effect of schisandrin and deoxyschisandrin on acute liver injury induced by carbon tetrachloride(CCl4)in mice and to study its mechanism.Methods Mice were randomly divided into five groups:normal group(0.5%carboxymethylcellulose sodium,0.5%CMC-Na),model group(0.5%CMC-Na),control group(bicyclol 300 mg·kg-1·d-1),schisandrin group(schisandrin 400 mg·kg-1·d-1),and deoxyschisandrin group(deoxyschisandrin 400 mg·kg-1·d-1),with 10 mice in each group,intragastrically twice a day for 7 d.One hour after the last administration,except for normal group,the mice of other groups were intraperitoneally injected with 0.3%CCl4 peanut oil solution for reproduction of acute liver injury model in mice.The serum and liver tissues of mice of each group were collected.The serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were measured by automatic biochemical analyzer.The malondialdehyde(MDA)and superoxide dismutase(SOD)levels in liver tissue were measured by biochemical kits.Results The serum levels of ALT in normal group,model group,control group,schisandrin group,and deoxyschisandrin group were(30.90±3.14),(3986.90±78.63),(387.00±24.39),(1914.70±89.35),and(2142.10±98.35)U·L-1,respectively;the serum levels of AST in the 5 groups were(191.50±18.02),(2337.70±80.34),(978.10±95.65),(1525.60±96.91),and(1405.30±92.31)U·L-1,respectively;the MDA levels in liver tissue in the 5 groups were(3.08±0.18),(8.67±0.28),(5.13±0.22),(6.63±0.28),and(6.18±0.34)μmol·g-1,respectively;the SOD levels in liver tissue in the 5 groups were(162.42±4.03),(102.86±3.55),(148.78±7.57),(132.83±6.42),and(138.21±4.94)U·mg-1,respectively.Comparison between model group and normal group or between control group,schisandrin group,deoxyschisandrin group and model group,the differences of the factors were significant(all P<0.01).Conclusion Schisandrin and deoxyschisandrin have protective effects on acute liver injury induced by CCl4 in mice,and its mechanism is related to anti-o
作者 王陈萍 宣东平 陈霞 乔进 窦志华 WANG Chen-ping;XUAN Dong-ping;CHEN Xia;QIAO Jin;DOU Zhi-hua(Department of Pharmacy,Nantong Third Affiliated Hospital of Nantong University,Nantong 226006,Jiangsu Province,China)
出处 《中国临床药理学杂志》 CAS CSCD 北大核心 2019年第8期791-794,共4页 The Chinese Journal of Clinical Pharmacology
基金 南通市应用研究计划基金资助项目(MS2015061) 南通市青年医学人才科研基金资助项目(WQ2015039).
关键词 五味子醇甲 五味子甲素 四氯化碳 急性肝损伤 schisandrin deoxyschisandrin carbon tetrachloride acute liver injury
作者简介 王陈萍(1988-),女,硕士,主管药师,主要从事药理学研究;通信作者:窦志华,主任中药师,副教授,硕士生导师,MP:13951319121,E-mail:zhihuadou@163.com。
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