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肠胃清抑制人脐静脉内皮细胞血管生成的实验研究 预览

Experimental study on Inhibitory Effect of Changweiqing on angi ogenesis of Human Umbilical Vein Endothelial Cells
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摘要 目的:研究肠胃清对人脐静脉内皮细胞血管生成的影响,并探讨其作用机理。方法:通过体外培养人脐静脉内皮细胞(HUVECs),采用细胞划痕实验、transwell迁移实验、小管形成实验检测肠胃清含药血清对HUVECs的迁移、管腔形成能力的影响。采用实时荧光定量PCR(RT-PCR)检测肠胃清含药血清对HUVECs内血管内皮生长因子A(VEGF-A)、碱性成纤维生长因子(bFGF)、基质金属蛋白酶2/9(MMP2/9)mRNA表达的影响。结果:细胞划痕、transwell迁移实验显示肠胃清含药血清抑制HUVECs的迁移(P<0.01)。小管形成实验显示肠胃清含药血清抑制HUVECs的成管能力(P<0.01)。RT-PCR结果显示肠胃清下调HUVECs内VEGF-A、bFGF、MMP2、MMP9 mRNA的表达(P<0.01),呈浓度依赖性。结论:肠胃清能抑制人脐静脉内皮细胞血管生成,可能与降低HUVECs内血管生成因子VEGF-A、bFGF、MMP2、MMP9的mRNA表达有关。 Objective:To study the effect of Changweiqing on angiogenesis of human umbilical vein endothelial cells and to explore its mechanism of action.Methods:Human umbilical vein endothelial cells(HUVECs)were cultured in vitro.The effects of gastrointestinal serum containing serum on the migration and lumen formation of HUVECs were examined by cell scratch test,transwell migration test and tubule formation test.Real-time quantitative PCR(RT-PCR)was used to detect vascular endothelial growth factor A(VEGF-A),basic fibroblast growth factor(bFGF)and matrix metalloproteinase 2/9(MMP2/9)in HUVECs.The effect of mRNA expression.Results:Cell scratches and transwell migration experiments showed that gastrointestinal clear drug serum inhibited the migration of HUVECs(P<0.01).The tubule formation experiment showed that the serum containing gastrointestinal tract inhibited the tube-forming ability of HUVECs(P<0.01).The results of RT-PCR showed that the expression of VEGF-A,bFGF,MMP2 and MMP9 mRNA in HUVECs was down-regulated(P<0.01)in a concentration-dependent manner.Conclusion:Intestinal clearing can inhibit the angiogenesis of human umbilical vein endothelial cells,which may be related to the decrease of mRNA expression of VEGF-A,bFGF,MMP2 and MMP9 in HUVECs.
作者 吴瑞影 许建华 WU Ruiying;XU Jianhua(The First Affiliated Hospital of University of Science and Technology,Anhui Hefei 230001,China;Putuo Hospital Affiliated of Shanghai University of Traditional Chinese Medicine,Shanghai 200333,China)
出处 《中医药临床杂志》 2019年第11期2116-2119,共4页 Clinical Journal of Traditional Chinese Medicine
关键词 肠胃清 肿瘤血管生成 人脐静脉内皮细胞 Changweiqing Tumor angiogenesis HUVECs
作者简介 吴瑞影,女。研究方向:中医药防治恶性肿瘤的基础和临床研究。E-mail:siyepiaoying@126.com。
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