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养阴口香合剂中枳壳的质量控制研究 预览

Quality Control of Aurantii Fructus in Yangyin Kouxiang Mixture
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摘要 目的研究并控制养阴口香合剂中枳壳的质量。方法采用薄层色谱法对枳壳进行鉴别研究;采用高效液相色谱法测定枳壳中柚皮苷和新橙皮苷的含量,色谱柱为Waters Xbridge C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(20∶80),柱温为30℃,流速为1.0 mL·min^-1,检测波长283 nm。结果薄层色谱斑点清晰,分离度较好;高效液相色谱测定柚皮苷对照品在0.05680μg~0.7100μg范围内线性关系良好(r=0.9999),平均回收率为100.21%;新橙皮苷对照品在0.03053μg~0.3817μg范围内线性关系良好(r=0.9999),平均回收率为98.33%。结论本研究方法准确可靠,重复性好,可用于养阴口香合剂中枳壳的质量控制。 Objective To study and control quality of Aurantii Fructus in Yangyin Kouxiang Mixture.Methods Thin layer chromatography(TLC)was used for qualitative identification of Aurantii Fructus.High performance liquid chromatography(HPLC)was used for content determination of naringin and neohesperidin in Aurantii Fructus(AF)on a Waters Xbridge C18(250 mm×4.6 mm,5μm),and the mobile phase was acetonitrile-water(20:80)at the column temperature of 30℃.The flow rate was 1.0 mL·min-1 and the detection wavelength was 283 nm.Results The TLC spots were clear and showed a good separation.HPLC showed the calibration curve of naringin had a good linear relationship in the mass range of 0.05680μg~0.7100μg(r=0.9999)and the average recovery was 100.21%;the calibration curve of neohesperidin had a good linear relationship in the mass range of 0.03053μg~0.3817μg(r=0.9999)and the average recovery was 98.33%.Conclusion The method is accurate with good reproducibility,which can be used for the quality control of Aurantii Fructus in Yangyin Kouxiang Mixture.
作者 郭璐玫 刘兴鹏 陈文碧 姜莲 陈凤 GUO Lumei;LIU Xingpeng;CHEN Wenbi;JIANG Lian;CHEN Feng(Guizhou Institute for Food and Drug Control,Guiyang 550004,China;Guizhou Wanshuntang Pharmaceutical Co.,Ltd.Guiyang 550018,China)
出处 《云南中医学院学报》 2019年第1期89-93,共5页 Journal of Yunnan University of Traditional Chinese Medicine
基金 国家药典委员会国家药品标准提高行动计划项目(编号:554)。
关键词 养阴口香合剂 枳壳 薄层色谱法 柚皮苷 新橙皮苷 高效液相色谱法 Yangyin Kouxiang Mixture Aurantii Fructus TLC naringin neohesperidin HPLC
作者简介 第一作者:郭璐玫(1987-),女,硕士,主管药师,研究方方向:中药检验及质量研究;通信作者:刘兴鹏,E-mail:24617309@qq.com。
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