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Cre/LoxP系统在眼部动物模型中的研究进展 预览

Research progress of Cre/LoxP system in the eyes of animal model
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摘要 Cre/LoxP系统在新型基因打靶中应用广泛,可对特定组织和器官中的基因进行改造,将特定的基因片段删除,以研究特定基因对生长发育的影响。Cre/LoxP系统是条件性、诱导性和时空特异性基因打靶策略的技术核心。研究Cre/LoxP系统的工作机制有助于获得视网膜不同细胞类型的Cre转基因小鼠模型,如视网膜双极细胞及神经节细胞上特异性表达的Cre工具鼠,可为特定组织单基因的缺失在生长和发育过程中的影响提供实验基础。本文着重对Cre/LoxP系统及眼部特异性Cre重组酶模型鼠进行阐述。 Cre/LoxP system has been widely used as new type of gene targeting,which can transform genes in specific tissues and organs,remove specific gene fragments,and help to study the effects of specific genes on growth and development.Cre/LoxP system is the core technology of conditional gene targeting,which induces gene targeting,and spatiotemporal specific gene targeting.The resource about the mechanism of the Cre/LoxP system is helpful to obtain the Cre transgenic mouse model on different cell types of the retina.The Cre transgenic mice with specific expression in retinal bipolar cells and ganglion cells can provide the experimental basis for the absence of specific tissue single genes during growth and development.This review focused on the Cre/LoxP system and specific Cre recombinase mouse model in eyes.
作者 娄翔峰(综述) 罗雪 沈吟(审校) Lou Xiangfeng;Luo Xue;Shen Yin(Eye Center,Renmin Hospital of Wuhan University,Wuhan 430060,China)
出处 《中华实验眼科杂志》 CAS CSCD 北大核心 2019年第9期759-763,共5页 Chinese Journal Of Experimental Ophthalmology
基金 国家自然科学基金项目(81470628、81270998) 湖北省卫健委青年人才项目(WJ2015Q014) 武汉市科技局晨光计划项目(2016070204010153)。
关键词 小鼠/Cre转基因 小鼠模型 视网膜 Mice,Cre transgenic Disease model,mice Retina
作者简介 通信作者:沈吟,Email:yinshen@whu.edu.cn。
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