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水稻三结构域多铜氧化酶基因家族生物信息学分析

Bioinformatics Analysis on the Gene Family of Three-domain Multi-copper Oxidases in Rice
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摘要 三结构域多铜氧化酶是生物体内非常重要的金属氧化酶,对机体生长和发育具有重要影响。为了系统地了解水稻三结构域多铜氧化酶基因家族的基本情况,本研究运用生物信息学方法对其成员进行了预测和分析;研究发现,以拟南芥三结构域多铜氧化酶基因At5g21105为靶序列,利用BlastP为工具在水稻基因组中共搜索到38个同源基因;这些基因的蛋白产物都依次含有Cu-oxidase3、Cu-oxidase和Cu-oxidase2三种结构域,各结构域中含有铜离子结合位点,二级结构具有α-螺旋和β-转角等基本特征,特推断它们都属于三结构域多铜氧化酶家族成员;进一步地,根据同源性程度将水稻多铜氧化酶基因家族成员划分为ClassⅠ和ClassⅡ两个亚家族,其中ClassⅠ包含31个基因,ClassⅡ包含7个基因。这些研究结果为进一步研究水稻多铜氧化酶基因家族成员的功能提供了参考依据。 Three-domain multi-copper oxidases, a very important metal oxidase in organism, are essential for the growth and development of organism. In order to understand the basic situation of multi-copper oxidase gene family in rice, in this study, bioinformatics methods were used to predict and analyze its family members. By performing a program of BlastP with a target sequence of At5g21105 encoding a three-domain multi-copper oxidase, 38 homologous proteins were identified from rice genome. Furthermore, these proteins products of these genes were predicted to contain three domains including Cu-oxidase3, Cu-oxidase and Cu-oxidase2 in order. In particular, the copper ion binding sites were found at every domain, and also, the secondary structure was characterized in alpha helix and beta turn. It was inferred that they would belong to the family of three-domain multi-copper oxidases. Furthermore, according to the degree of homology, the polycopper oxidase gene family in rice was divided into two subgroups, with 31 gene for ClassⅠand 7 for ClassⅡrespectively. The results of our study might provide beneficial information for further functional investigation on its family members in rice.
作者 王有成 谭炎宁 张超 殷小林 何强 肖应辉 邓华凤 Wang Youcheng;Tan Yanning;Zhang Chao;Yin Xiaolin;He Qiang;Xiao Yinghui;Deng Huafeng(College of Agronomy,Hunan Agricultural University,Changsha,410128;State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha,410125;Longping Branch of Graduate School,Hunan University,Changsha,410125)
出处 《分子植物育种》 CAS CSCD 北大核心 2019年第5期1385-1392,共8页 Molecular Plant Breeding
基金 国家863课题(2014AA10A604) 湖南农业科技创新资金(2017XC09)共同资助.
关键词 水稻 多铜氧化酶 基因家族 结构域 生物信息学分析 Rice Multi-copper oxidase Gene family Domain Bioinformatics analysis
作者简介 通讯作者:邓华凤,dhf@hhrrc.ac.cn;通讯作者:肖应辉,xiaoyh@hunau.edu.cn;通讯作者:何强,heqiang@hhrrc.ac.cn.
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