Three-domain multi-copper oxidases, a very important metal oxidase in organism, are essential for the growth and development of organism. In order to understand the basic situation of multi-copper oxidase gene family in rice, in this study, bioinformatics methods were used to predict and analyze its family members. By performing a program of BlastP with a target sequence of At5g21105 encoding a three-domain multi-copper oxidase, 38 homologous proteins were identified from rice genome. Furthermore, these proteins products of these genes were predicted to contain three domains including Cu-oxidase3, Cu-oxidase and Cu-oxidase2 in order. In particular, the copper ion binding sites were found at every domain, and also, the secondary structure was characterized in alpha helix and beta turn. It was inferred that they would belong to the family of three-domain multi-copper oxidases. Furthermore, according to the degree of homology, the polycopper oxidase gene family in rice was divided into two subgroups, with 31 gene for ClassⅠand 7 for ClassⅡrespectively. The results of our study might provide beneficial information for further functional investigation on its family members in rice.
Molecular Plant Breeding