期刊文献+

红景天苷对肥胖小鼠胰腺组织脂质沉积及PI3K/Akt信号的影响

Effects of Salidroside on Pancreatic Lipid Deposition and PI3K/Akt Pathway in Obese Mice
收藏 分享 导出
摘要 目的:研究红景天苷(salidroside,SAL)对肥胖小鼠胰腺脂质沉积及磷脂酰肌醇-3-激酶(phosphatidylinositol 3-kinase,PI3K)/Akt信号的影响。方法:采用高脂饲料喂养法(high-fat diet,HFD)建立肥胖小鼠模型,随机分为模型组和SAL(100 mg·kg^-1·d^-1)治疗组,同时设立正常对照组,连续给药8周;治疗期间检测体质量与血糖变化,治疗结束后解剖小鼠,收集胰腺组织行HE染色病理切片制作,以生化分析法对胰腺组织中甘油三酯(triglyceride,TG)含量进行检测,以Western blot法对胰腺组织PI3K/Akt信号水平进行检测。结果:SAL治疗可缓解HFD引起的体质量增加和血糖升高,病理切片和TG含量检测结果显示SAL可减少胰腺组织的异位脂肪沉积,Western blot结果提示SAL可增加胰腺组织PI3K/Akt信号的关键分子Akt和糖原合成酶激酶β的磷酸化水平。结论:SAL干预可减少HFD肥胖小鼠胰腺组织脂质沉积,并激活胰腺PI3K/Akt信号。 Objective To study the effects of salidroside(SAL) on pancreatic lipid deposition and phosphatidylinositol 3-kinase(PI3 K)/Akt signal in obese mice. Methods Obese mouse models were established by high-fat diet(HFD) and randomly divided into model group and SAL(100 mg·kg^-1·d^-1) treatment group, and normal control group was established at the same time. The drug was given for 8 weeks. The body mass and blood glucose were measured during the treatment. The mice were dissected after the treatment, and the pancreatic tissue was collected and stained with HE staining. The content of triglyceride(TG) in pancreatic tissue was detected by biochemical analysis. The PI3 K/Akt signal level of pancreatic tissue was detected by Western blot. Results SAL treatment can alleviate the increase in body mass and blood glucose caused by HFD. The results of pathological section and TG content showed that SAL can reduce ectopic fat deposition in pancreatic tissue. Western blot results suggested that SAL can increase the phosphorylation levels of Akt and glycogen synthase kinase β, key molecules of PI3 K/Akt signal in pancreatic tissue. Conclusion SAL intervention can reduce pancreatic lipid deposition and activate pancreatic PI3 K/Akt signal in HFD-induced obese mice.
作者 郑涛 王启斌 赵艳 陈黎 ZHENG Tao;WANG Qi-bin;ZHAO Yan;CHEN Li(Department of Pharmacy, Taihe Hospital, Hubei University of Medicine;Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China)
出处 《湖北医药学院学报》 CAS 2019年第4期320-324,F0003共6页 Journal of Hubei University of Medicine
基金 国家自然科学基金青年基金(81703582) 湖北省自然科学基金(2016CFB153) 湖北省卫生健康科研基金(WJ2019M057) 湖北医药学院附属太和医院博士科研启动基金(2016BSQD03,2016BSQD05).
关键词 红景天苷 肥胖 胰腺 脂质沉积 PI3K/Akt信号 Salidroside Obesity Pancreas Lipid deposition PI3K/Akt pathway
作者简介 郑涛(1985-),男,湖北黄冈人,医学博士,主管药师,研究方向:糖尿病发病机制及天然药物干预作用。Email:ztice@fbxmail.com;通信作者:陈黎(1973-),女,湖北十堰人,医学博士,主任药师,研究方向:中药资源及制剂研发。E-mail:chenli0201@sina.com.
  • 相关文献
投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈