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剑麻感染烟草疫霉菌后几种重要防御酶活性的变化 预览

Several Cases of Sisal( H.11648) Infection with Phytophthora nicotianae Changes in Important Defense Enzyme Activities
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摘要 对剑麻(H.11648)叶片接种烟草疫霉菌(Phytophthora nicotianae)前后7种重要防御酶的活性变化规律进行了研究。结果表明:对照(只对叶片进行针刺,喷无菌水,不接种烟草疫霉菌)剑麻苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、过氧化物酶(POD)、几丁质酶(CHT)、&-1,3葡聚糖酶(GLU)等7种酶活性在120 h时之内没有明显变化。接种烟草疫霉菌的剑麻叶片,PAL、PPO和POD活性随着时间的推移呈现先上升后下降的趋势,96 h时达到最高值;SOD活性随着时间的推移呈现先下降后上升的趋势,48 h时降到最低值;CAT活性随着时间的推移呈现先上升后下降的趋势,48 h时达到最高值;CHT和GLU活性随着时间的推移呈现逐步上升的趋势,120 h后仍呈上升趋势。 Studies were conducted on the activities of seven important defense enzymes activities in sisal(H.11648) leaves before and after inoculation with Phytophthora nicotianae. The results showed that phenylalanine ammonia enzyme solution(PAL),superoxide disproportionation enzyme(SOD),polyphenol oxidase(PPO),catalase(CAT),peroxidase(POD),chitinase(CHT) and &-1, 3 glucanase(GLU) contents of sisal in the control group(not inoculated with P. nicotianae) did not change significantly within 120 h. The enzyme activity of PAL, PPO and POD increased first and subsequently decreased with time, and reached their highest values at 96 h. The activity of CAT showed the similar dynamic,but reached its highest level at 48 h. The activity of SOD decreased first and later increased with time, and reached its lowest activity level at 48 h. CHT and GLU activities showed a gradually increasing trend over time, which was still observed even after 120 h.
作者 郑金龙 易克贤 习金根 黄兴 吴伟怀 高建明 张世清 陈河龙 贺春萍 粱艳琼 陆英 ZHENG Jinlong;YI Kexian;XI Jingen;HUANG Xing;WU Weihuai;GAO Jianming;ZHANG Shiqing;CHEN Helong;HE Chunping;LIANG Yanqiong;LU Ying(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture and Rural Affairs,P.R.China,Haikou,Hainan 571101,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China)
出处 《中国麻业科学》 2019年第5期210-216,共7页 Plant Fiber Sciences in China
基金 国家重点研发计划项目(2018YFD0201100) 国家麻类产业技术体系建设专项(CARS-16-E16) 国家自然科学基金面上项目(31771849) 中央级公益性科研院所基本科研业务费专项(1630042019028).
关键词 剑麻 烟草疫霉菌 酶活性 sisal Phytophthora nicotianae enzyme activity
作者简介 郑金龙(1982-),男,助理研究员,硕士,研究方向为植物病理。E-mail:zhengjinlong_36@163.com;通讯作者:易克贤(1964-),男,研究员,博士,研究方向为分子抗性育种。E-mail:yikexian@126.com;通讯作者:贺春萍(1974-),女,研究员,硕士,研究方向为植物病理。E-mail:hechunppp@163.com.
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