期刊文献+

HSP110家族在各发育阶段小鼠睾丸、附睾中的表达及与激素调控的关系 预览

Expressions of HSP110 family members in the testes and epididymis of mice at different stages of development and their regulation by hormones
在线阅读 免费下载
收藏 分享 导出
摘要 目的研究HSP110家族在发育中的小鼠睾丸和附睾中的表达及其是否受激素调控。方法收集不同周龄(出生后14、21、28、35、42、49、70、90d)小鼠睾丸及附睾组织,每周龄3只,应用RT-PCR检测HSP110家族成员在不同发育阶段小鼠睾丸、附睾中的基因表达水平。建立小鼠去势实验模型,应用RT-PCR检测HSP110家族基因在假手术组、去势组及去势后补充睾酮组小鼠附睾中的表达水平。结果HSP110家族成员随着发育过程的进行其基因表达水平发生变化:HSPA4、HSPA4l、HSPH1的基因在出生后不同发育阶段的小鼠睾丸中表现出先增后降,逐渐趋于平稳的趋势;HSP110家族4个成员在出生后不同发育阶段小鼠附睾中的表达也是先增后降,逐渐平稳。小鼠去势实验结果显示HSPA4和HSPA4l随着激素水平的降低其基因表达水平降低(P<0.05),补充外源性激素后其表达恢复正常。结论HSP110家族成员的基因表达水平受发育调控的影响,HSPA4和HSPA4l基因的表达受激素调控。 Objective To study the expressions of the members of HSP110 family in the testis and epididymis of mice at different stages of development and whether they are regulated by hormones.Methods The testicular and epididymis tissues of mice at different ages (14,21,28,35,42,49,70,and 90 days after birth,3 mice at each age) were collected for RT-PCR detection of the expression levels of HSP110 family members.Forty-eight mice were randomized into 3 groups for sham operation,castration, or castration with testosterone injections every other day (starting at 7 days after castration),and at 1,3,5,and 7 days after first testosterone injection,the expressions of HSP110 family in the epididymis were detected using RT-PCR.Results The mRNA expression levels of HSP110 family members underwent obvious variations with the development of the mice: HSPA4,HSPA4l and HSPH1 expressions in the testicles of the mice first increased and then decreased,and gradually became stable;they also exhibited similar temporal patterns of changes in the epididymis.In the castrated mice,the mRNA expressions of HSPA4 and HSPA4l in the epididymis decreased significantly with the reduction of serum hormone levels (P<0.05),and became normal after the supplementation of exogenous hormone.Conclusion The expression levels of HSP110 family are affected by developmental regulation,and the expressions of HSPA4 and HSPA4l are under the regulation by hormones.
作者 戎成婷 杜子伟 刘娟 吴新安 RONG Chengting;DU Ziwei;LIU Juan;WU Xinan(Department of Pharmacy,Hefei BOE Hospital,Anhui,Hefei 230041,China;Central Laboratory of Yantai Yuhuangding Hospital,Yantai 264000,China)
出处 《南方医科大学学报》 CAS CSCD 北大核心 2019年第9期1083-1088,共6页 Journal of Southern Medical University
基金 国家自然科学基金(81501313) 中华医学会临床医学科研专项基金-生殖医学青年医师研究与发展项目(18010200749).
关键词 HSP110家族 发育 细胞凋亡 激素调控 HSP110 family development apoptosis hormone regulation
作者简介 第一作者:戎成婷,主管药师,硕士研究生,E-mail:rongchengting@boe.com.cn;第一作者:杜子伟,主管药师,硕士研究生;通信作者:吴新安,副主任药师,博士,E-mail:wuxinan@boe.com.cn.
  • 相关文献

参考文献3

二级参考文献60

  • 1Meng X,Lindahl M,Hyvonen ME,et al.Regulation of cell fate decision of undifferentiated spermatogonia by GDNF [ J ].Science,2000,287(5457):1489-1493. 被引量:1
  • 2McLachlan RI,ODonnell L,Meachem SJ,et al.Hormonal regulation of spermatogenesis in primates and man:insights for development of the male contraceptive [ J ].J Androl,2002,23(2):149-162. 被引量:1
  • 3Themmen APN,Huhtaniemi IT.Mutations of gonadotropins and gonadotropin receptors:elucidating the physiology and pathophysiology of pituitary-gonadal function [ J ].Endoor Rev,2000,21(5):551-583. 被引量:1
  • 4Layman LC.Mutations in the follicle-stimulating hormone-beta(FSH beta)and FSH receptor genes in mice and humans[ J].Semin Reprod Med,2000,18(1):5-10. 被引量:1
  • 5Ramaswamy S,Plant TM.Operation of the follicle-stimulating hormone(FSH)-inhibin B feedback loop in the control of primate spermatogenesis[ J ].Mol Cell Endocrinol,2001,180(1-2):93-101. 被引量:1
  • 6Kinniburgh D,Anderson RA,Baird DT.Suppression of spermatogenesis with desogestrel and testosterone pellets is not enhanced by addition of finasteride[ J].J Androl,2001,22(1):88-95. 被引量:1
  • 7葛秦生主编.临床生殖内分泌学--男性与女性[M].北京:科学技术文献出版社,2001.762-800. 被引量:1
  • 8Wang C,Swerdloff RS.Male contraception [ J ].Best Pract Res Clin Obstet Gynaecol,2002,16(2):193-203. 被引量:1
  • 9Plant TM,Marshall GR.The functional significance of FSH in spermatogenesis and the control of its secretion in male primates [J].Endocr Rev,2001,22(6):764-786. 被引量:1
  • 10ODonnell L,Narula A,Balourdos G,et al.Impairment of spermatogonial development and spermiation after testosterone-induced gonadotropin suppression in adult monkeys(Macaca fascicularis)[J].J Clin Endoorinol Metab,2001,86(4):1814-1822. 被引量:1

共引文献29

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈