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二陈汤及其不同组方对Lewis肺癌小鼠免疫功能的影响 预览

Effects of Erchen decoction and its different prescriptions on immune function in Lewis lung cancer mice
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摘要 目的探讨二陈汤其不同组方对Lewis肺癌小鼠免疫功能的影响。方法60只C57BL/6小鼠建立Lewis肺癌小鼠模型,随机分为模型组、二陈汤组、二陈汤益气养阴组(二陈汤加生黄芪、知母)、二陈汤解毒散结组(二陈汤加山慈菇、姜黄)、二陈汤破血逐瘀组(二陈汤加三棱、莪术)、顺铂组,每组各10只。模型组灌胃生理盐水,二陈汤及其组方分别灌胃二陈汤及相应组方溶液,顺铂组腹腔注射顺铂溶液,每日1次,连用3天,同时灌胃生理盐水,每日1次,连续12天。测量小鼠重、脾重和胸腺重,计算脾指数和胸腺指数;全自动血液流变测试仪测定外周血细胞数量;流式细胞术测定小鼠脾脏T淋巴细胞亚群水平;CCK-8法测定脾淋巴细胞增殖能力。结果与模型组相比,二陈汤及其组方脾淋巴细胞刺激指数均显著升高,顺铂组明显下降,二陈汤组脾指数、胸腺指数、外周血细胞及CD3^+、CD4^+、CD8^+显著增高,益气养阴组脾指数、白细胞、血小板及CD4^+升高,解毒散结组外周血细胞及CD3^+、CD4^+显著增高,破血逐瘀组胸腺指数、外周血细胞及CD3^+、CD4^+增高,顺铂组红细胞、血小板及CD4^+/CD8^+增高;与顺铂组相比,二陈汤及其组方脾指数、刺激指数及白细胞均显著升高,二陈汤组红细胞及CD3^+、CD4^+、CD8^+显著升高,破血逐瘀组CD8^+升高,CD4^+/CD8^+方面,二陈汤组、益气养阴组、破血逐瘀组均降低;二陈汤及其组方间比较,二陈汤组胸腺指数及CD3^+、CD4^+均显著高于益气养阴组,二陈汤组CD3^+高于解毒散结组,二陈汤组CD3^+、CD4^+高于破血逐瘀组,破血逐瘀组胸腺指数高于益气养阴组,解毒散结组CD4^+/CD8^+高于破血逐瘀组。以上差异均具有统计学意义(P<0.01或P<0.05)。结论二陈汤及其不同组方均对Lewis肺癌小鼠的免疫功能指标有不同程度的提高,均可增加机体免疫功能,其中,以二陈汤增强免疫功能的作用最为明显。 Objective To explore the effects of Erchen decoction(ECD)and its different prescriptions on immune function of Lew-is lung cancer mice.Methods Lewis lung cancer model was established in 60 C57BL/6 mice.They were randomly divided into model group,ECD group,ECD Yiqi Yangyin(YQYY)group(ECD with Radix Astragali and Rhizoma Anemarrhenae),ECD Jiedu Sanjie(JDSJ)group(ECD with Pseudobulbus Cremastrae seu Pleiones and Rhizoma Curcumae Longae),ECD Poxue Zhuyu(PXZY)group(ECD with Rhizoma Sparganii and Rhizoma Curcumae)and cisplatin group,each with 10 mice.The model group was treated by gavage with normal saline,the ECD group and its different prescription groups were treated by gavage with ECD and its corresponding prescriptions respectively,and the cisplatin group was intraperitoneally injected with cisplatin solution.The treatment was for once a day for 3 consecutive days.Simultaneously,they were treated with normal saline by gavage once a day for 12 consecutive days.The body weight,spleen weight and thymus weight of mice were measured,and spleen index and thymus index were calculated.The number of peripheral blood cells was measured by automatic blood rheometer.Flow cytometry was used to measure T lymphocyte subsets in spleens of mice.Proliferative ability of splenic lymphocytes was measured by CCK-8 method.Results Compared with the model group,the stimulating index of splenic lymphocytes in the ECD and its prescription groups was significantly increased,while that in the cisplatin group was significantly decreased.The spleen and thymus indexes,peripheral blood cells,CD3^+,CD4^+and CD8^+in the ECD group were increased significantly.The spleen index,WBC,PLT and CD4^+in the YQYY group were increased.The peripheral blood cells,CD3^+and CD4^+in the JDSJ group were significantly increased.The thymus index,peripheral blood cells,CD3^+and CD4^+in the PXZY group were increased.The red blood cells(RBC),PLT and CD4^+/CD8^+in the cisplatin group were increased.Compared with the cisplatin group,the spleen index,stimulating index and
作者 王子卿 李燕 王芬 江洋 张弘 王玥慧 张燕娜 黄璐璐 杜倩倩 WANG Ziqingy;LI Yan;WANG Fen(Beijing University of Chinese Medicine,Beijing 100029,China)
出处 《环球中医药》 CAS 2019年第10期1463-1470,共8页 Global Traditional Chinese Medicine
基金 国家自然科学基金面上项目(81573798).
关键词 二陈汤 二陈汤不同组方 LEWIS肺癌小鼠 免疫功能 T淋巴细胞亚群 免疫器官指数 外周血细胞 脾淋巴细胞增殖能力 Erchen decoction Different prescriptions of Erchen decoction Lewis lung cancer mice Immune function T lymphocyte subsets Immune organ index Peripheral blood cells Splenic lymphocyte proliferative ability
作者简介 王子卿(1995-),女,2017级在读硕士研究生。研究方向:中西医结合治疗肿瘤的临床与基础研究。E-mail:503132547@qq.com;通信作者:王芬(1975-),女,博士,主任医师,副教授,博士生导师。研究方向:中西医结合治疗肿瘤的临床与基础研究。E-mail:wangfen919@siiia.com;江洋,硕士研究生;张弘,博士研究生;王玥慧,硕士研究生;张燕娜,硕士研究生;黄璐璐,硕士研究生;杜倩倩,硕士研究生.
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