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Myogenin在不同发育阶段小鼠骨骼肌中的表达差异 预览

Difference of Myogenin Expression in the Skeletal Muscle of Mice at Different Developmental Stages
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摘要 Myogenin是骨骼肌生长发育过程中的重要调控因子之一。采用H.E.染色法观察小鼠骨骼肌组织不同发育阶段的形态特征,通过免疫组织化学法观察在不同发育阶段因子是否表达,并通过实时荧光定量PCR技术对Myogenin在不同发育时期的表达量进行分析,旨在研究不同发育阶段小鼠骨骼肌中Myogenin的表达差异。结果表明,Myogenin在不同发育阶段小鼠骨骼肌的表达位置均为细胞质,且在4个阶段的表达量间存在显著差异,幼年时期表达量最高。 Myogenin is one of the important regulation factors in the growth and development of skeletal muscle.The purpose of this experiment was to study the expression of Myogenin in the skeletal muscle of mice at different stages of development.In this study,H.E.staining was used to observe the morphological characteristics of skeletal muscle tissue in mice at different development stages,immunohistochemical method was used to observe whether the factor was expressed at different development stages,and the expression of Myogenin in different development stages was analyzed by real-time fluorescence quantitative PCR.The results showed that the expression location of Myogenin in skeletal muscle of mice at different development stages was cytoplasm,and the expression was significantly different in the four stages,with the highest expression in the early stage.
作者 任华伟 薛霖莉 曹靖 李艺雷 冀云燕 罗小毛 于秀菊 赫晓燕 王海东 REN Huawei;XUE Linli;CAO Jing;LI Yilei;JI Yunyan;LUO Xiaomao;YU Xiuju;HE Xiaoyan;WANG Haidong(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China;College of Information,Shanxi Agricultural University,Taigu 030800,China;Department of AnimalHusbandry and Veterinary,Beijing Vocational College of Agriculture,Beijing 102442,China)
出处 《山西农业科学》 2019年第8期1485-1489,1500共6页 Journal of Shanxi Agricultural Sciences
基金 山西省重点研发项目(201803D221022-2) 山西省优秀人才科技创新项目(201805D211012) 国家自然科学基金项目(31772690).
关键词 不同发育阶段 小鼠 骨骼肌 MYOGENIN different developent stages mice skeletal muscle Myogenin
作者简介 任华伟(1993-),女,山西代县人,在读硕士,研究方向:动物肌肉发育及脂肪代谢;通信作者:王海东。
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