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高效液相色谱-蒸发光散射法测定翘嘴红鲌肌肉中磷脂的组成 预览

Determination of Phospholipid Composition in the Muscle of Ergthroculter ilishaeformis by High Performance Liquid Chromatography with Evaporative Light Scattering Detection
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摘要 为建立快速、灵敏检测翘嘴红鲌(Ergthroculter ilishaeformis)肌肉中各磷脂组分的方法,采用氯仿-甲醇提取翘嘴红鲌肌肉脂肪,利用高效液相色谱-蒸发光散射测定方法,对翘嘴红鲌肌肉中磷脂组成进行检测。检测条件如下:以Chromolith■Performan-ce-Si型正相硅胶色谱分析柱(100 mm×4.6 mm)作为分离柱,正己烷-异丙醇-13%乙酸溶液为流动相体系,三元梯度洗脱,流速1.5 mL/min,柱温30℃,进样量20μL,蒸发光散射检测器漂移管温度70℃,雾化气(氮气)压力为320 kPa。在此条件下,翘嘴红鲌肌肉中各磷脂组分均能完全分离,且各磷脂组分在相应范围内峰面积与浓度线性关系良好。方法精密度高,变异系数均小于3.0%,平均回收率为88.38%~107.41%,相对标准偏差为0.40%~4.95%。本方法操作简单、分析速度快、检测灵敏度高、精密度好、结果准确可靠,适用于翘嘴红鲌等淡水鱼中磷脂含量的测定。 This study was undertaken in order to establish a rapid and sensitive method for detecting the content of phospholipids in the muscle of Ergthroculter ilishaeformis. Chloroform methanol was used to extract lipids, and the composition of phospholipids was detected by high performance liquid chromatography with evaporative light scattering detection(HPLC-ELSD). A Chromolith■ Performance-Si type positive phase silica gel column(100 mm × 4.6 mm) was used as the separation column, and gradient elution was performed using a ternary mobile phase consisting of n-hexaneisopropanol-13% acetic acid solution at a flow rate of 1.5 mL/min. The column temperature was set at 30 ℃, and sample volume at 20 μL. The ELSD drift tube temperature was 70 ℃, and atomization gas(nitrogen) pressure 320 kPa. All the phospholipid components were completely separated, and the peak area of each had a good linear relationship with its concentration in the tested ranges. The method had high precision with coefficient of variation(CV) less than 3%. The average recovery was 88.38%–107.41% with relative standard deviation(RSD) of 0.40%–4.95%. This method is simple,fast, sensitive, reproducible, accurate and reliable and can be suitable for the determination of phospholipid contents in freshwater fish such as E. ilishaeformis.
作者 韩迎雪 林婉玲 杨少玲 李来好 黄卉 杨贤庆 王锦旭 吴燕燕 翟红蕾 郝淑贤 HAN Yingxue;LIN Wanling;YANG Shaoling;LI Laihao;HUANG Hui;YANG Xianqing;WANG Jinxu;WU Yanyan;ZHAI Honglei;HAO Shuxian(Key Laboratory of Aquatie Product Processing,Ministry of Agriculture,National Research and Development Center for AquaticProduct Processing,South China Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China;Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300,China)
出处 《食品科学》 EI CAS CSCD 北大核心 2019年第14期223-228,共6页 Food Science
基金 “十三五”国家重点研发计划重点专项(2016YFD0400201-6) 现代农业产业技术体系建设专项(CARS-46) “扬帆计划”引进创新创业团队专项资助项目(2015YT02H109).
关键词 翘嘴红鲌 磷脂 高效液相色谱 蒸发光散射 Ergthroculter ilishaeformis phospholipid high performance liquid chromatography evaporative light scattering detection
作者简介 第一作者:韩迎雪(1993-)(ORCID:0000-0002-9792-3893),女,硕士研究生,研究方向为水产品加工。E-mail:15737935907@163.com;通信作者:李来好(1963-)(ORCID:0000-0001-9371-9230),男,研究员,博士,研究方向为水产品精深加工与质量安全。E-mail:laihaoli@163.com.
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