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动物源细胞外基质中糖胺聚糖物质的检测方法 预览

Methods for detecting glycosaminoglycan in the extracellular matrix of animal cells
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摘要 背景:作为每个组织和器官中的细胞分泌产物,细胞外基质凭借其独一无二的生物学特性成为了临床应用中一种独特的再生型生物材料。糖胺聚糖作为细胞外基质中的重要活性物质之一,具有诸多优良的生物学特性。目的:整理总结近年来国内外对细胞外基质中糖胺聚糖检测方法的研究进展,并对各方法进行了对比,为优化细胞外基质糖胺聚糖检测方法提供帮助和方法参考。方法:通过输入关键词“糖胺聚糖检测、细胞外基质、determination methods of glycosaminoglycan、Animal-derived extracellular matrix”,从PubMed、知网等文献搜索引擎共搜索出2 037篇相关文献,筛选并整理63篇文献,总结出常见的糖胺聚糖检测方法。根据阅读文献时总结的思路,提出基于现有提取检测糖胺聚糖方法的改进方案,并设计对比实验验证。结果与结论:糖胺聚糖对动物源细胞外基质生物学性能起着至关重要的作用,可赋予细胞外基质材料及产品优异的生物活性与促修复功能。检测材料中糖胺聚糖含量可指导细胞外基质材料的工艺优化,因此糖胺聚糖检测方法至关重要。目前糖胺聚糖的检测方法主要有高效液相色谱法、糖醛酸分析法、乙醣胺定量分析法与二甲基亚甲基蓝检测法,以上方法均存在一定的局限性。基于已有的研究成果,针对二甲基亚甲基蓝检测法中的样本消化、糖胺聚糖提取等步骤做了改进,大幅度提升了检测结果的准确度,为其他糖胺聚糖检测方法提供了思路和参考。 BACKGROUND: As a cell secretory product in every tissue and organ, the extracellular matrix, by virtue of its unique biological properties, has become a unique regenerative biomaterial in clinical applications. As one of the important active substances in the extracellular matrix, glycosaminoglycan has remarkable biological characteristics.OBJECTIVE: To review the research progress of the detection methods of glycosaminoglycan in extracellular matrix at home and abroad and compare these methods to provide reference information to optimize the methods of detecting glycosaminoglycan in the extracellular matrix. METHODS: A total of 2 037 publications were retrieved by searching CNKI database using search terms “glycosaminoglycan”,“extracellular matrix” and by searching PubMed using search terms “determination methods of glycosaminoglycan”“Animal-derived extracellular matrix”. Sixty-three literatures were screened and included in the final analysis. The common methods of detecting glycosaminoglycan were summarized. According to the ideas summarized during the literature review, the improved method for glycosaminoglycan extraction and detection was proposed and a comparative study was designed to validate the improved method. RESULTS AND CONCLUSION: Glycosaminoglycan plays a vital role in the biological properties of animal-derived extracellular matrix and can confer superior biological activity and prosthetic function to extracellular matrix materials and products. Detection of glycosaminoglycan content in the extracellular matrix material can help to optimize extracellular matrix material. Therefore, the method of detecting glycosaminoglycan content in the extracellular matrix is of great importance. The currently available methods of detecting glycosaminoglycan include high performance liquid chromatography, uronic acid analysis, quantitative analysis of acetaminophen, and dimethyl methylene blue detection. All of these methods have certain limitations. Based on the existing research results, we onl
作者 张伟 陈俊霖 胡康 Zhang Wei;Chen Junlin;Hu Kang(Guanhao Biotech Co.,Ltd.,National Engineering Laboratory for Regenerative Medical Implantable Devices,Guangzhou 510530,Guangdong Province,China;Guangzhou Keyue Biotech Co.,Ltd.,Guangzhou 510530,Guangdong Province,China)
出处 《中国组织工程研究》 CAS 北大核心 2019年第30期4861-4867,共7页 Journal of Clinical Rehabilitative Tissue Engineering Research
基金 国家重点研发计划资助(2016YFC1103200).
关键词 细胞外基质 糖胺聚糖 临床应用 检测方法 生物学特性 制作工艺 二甲基亚甲基蓝检测 高效液相色谱法 extracellular matrix glycosaminoglycan clinical application detection biological characteristics production process dimethyl methylene blue detection high performance liquid chromatography
作者简介 张伟,男,1979年生,山东省济宁市人,汉族,北京大学在读博士,主要从事生物医用材料与医疗器械研究。
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