目的观察红景天苷治疗慢性肾衰竭大鼠肾功能减退的效果,并探讨其可能的机制。方法将实验大鼠随机分为模型组、红景天苷组及假手术组。模型组和红景天苷组大鼠采用切除5/6肾脏法建造慢性肾衰竭大鼠模型。红景天苷组给予红景天苷水溶液灌胃,模型组及假手术组给予蒸馏水灌胃。造模完成后通过HE染色法观察肾组织病理变化,全自动生化仪测定血清肌酐(serum creatinine,SCr)、尿素氮(blood urea nitrogen,BUN)水平以了解肾脏损伤的程度,试剂盒检测肾组织中丙二醛(superoxide dismutase,SOD)、超氧化物歧化酶(malondialdehyde,MDA)水平。结果模型组血浆SCr和BUN水平显著高于假手术组,肾组织SOD水平显著低于假手术组,MDA水平显著高于假手术组( P < 0.05);红景天苷组血浆SCr和BUN水平显著低于模型组,但仍高于假手术组,红景天苷组肾组织SOD水平高于模型组,但仍低于假手术组,红景天苷组肾组织MDA水平显著低于模型组,但仍高于假手术组( P < 0.05)。结论红景天苷可以有效缓解慢性肾衰竭大鼠的氧化应激水平,对肾脏有保护作用,可能是通过抑制抗氧化酶活性实现的。
Objective To investigate the effect of the Chinese herb Salidroside on chronic renal failure in rats, and to explore its possible mechanism. Methods Experimental animals were randomly assigned as chronic renal failure(CRF) group with 5/6 nephrectomy, Salidroside treatment group and sham-operated control group. Animals in the former two groups were subjected to 5/6 kidney resection to construct CRF rat models. Salidroside group received Salidroside for 4 weeks. The other two groups were given distilled water. At the end of the 4 weeks, kidney tissues were assessed by histopathologic analyses. The levels of serum creatinine(SCr) and blood urea nitrogen(BUN) were measured by automatic biochemical analyzer to estimate the renal function. Malondialdehyde(MDA) and superoxide dismutase(SOD) levels in the kidney tissues were measured by the kit. Results Compared with control group, the levels of serum BUN, SCr and MDA in the other two groups were significantly increased, whereas the level of SOD was declined( P <0.05). Compared with CRF group, the levels of serum BUN, SCr and MDA in the Salidroside group were obviously lower, whereas the level of SOD was higher( P < 0.05). Conclusion Salidroside supplementation can effectively alleviate the oxidative stress level and provide protective effect in CRF rats, which may be achieved by the suppression of the antioxidant enzyme activities.
Journal of Hebei Medical University