期刊文献+

兔源肺炎克雷伯氏菌重组酶聚合酶扩增检测方法的建立 预览

Establishment of a RPA Assay for Detecting Klebsiella pneumoniae of Rabbit Origin
在线阅读 下载PDF
收藏 分享 导出
摘要 为建立一种检测兔源肺炎克雷伯氏菌(Klebsiella pneumoniae)重组酶聚合酶扩增方法(recombinase polymerase amplification,RPA),根据肺炎克雷伯氏菌的phoE基因保守序列设计引物,扩增片段大小为277bp,并对反应条件进一步优化,最终建立了适宜于快速准确检测兔源肺炎克雷伯氏菌的重组酶聚合酶等温扩增方法。该方法可特异性检测出兔源肺炎克雷伯氏菌,最低检出细菌量为8.3×101CFU/mL,灵敏度比传统PCR高100倍。本研究建立的肺炎克雷伯氏菌RPA检测方法特异性强、操作简单,从而为生产中的兔源肺炎克雷伯氏菌现场快速检测提供了一种新方法。 In order to establish a recombinase polymerase amplification(RPA)assay for detecting Klebsiella pneumonia of rabbit origin,a pair of primers were designed based on the conservative sequence of PhoE gene of Klebsiella pneumoniae,and the fragment with the length of 277 bp was amplified,then the reaction conditions were further optimized,finally,the RPA assay was established,by which,the Klebsiella pneumoniae of rabbit origin could be specifically detected with the minimum bacteria of 8.3×10^1 CFU/mL,and its sensitivity was 100 times higher than that of traditional PCR. Therefore,the method established in the study had characteristics of strong specificity and simple operation,which would provide a new method for rapid detection of Klebsiella pneumoniae of rabbit origin.
作者 赵巧雅 马秀丽 刘存霞 史玉颖 胡峰 王贵升 兰邹然 黄兵 Zhao Qiaoya;Ma Xiuli;Liu Cunxia;Shi Yuying;Hu Feng;Wang Guisheng;Lan Zouran;Huang Bing(Poultry Institute,Shandong Academy of Agricultural Science,Jinan,Shandong 250023,China;Shandong Animal Disease Prevention and Control Center,Jinan,Shandong 250022,China)
出处 《中国动物检疫》 CAS 2019年第7期82-85,共4页 China Animal Health Inspection
基金 山东省现代农业产业技术体系项目(SDAIT-21-06) 山东省农业科学院农业科技创新工程(CXGC2016B14) 2019年山东省农业重大应用技术创新项目.
关键词 肺炎克雷伯氏菌 重组酶聚合酶扩增 快速检测 rabbit Klebsiella pneumoniae RPA rapid detection
作者简介 通信作者:兰邹然;通信作者:黄兵.
  • 相关文献

参考文献9

二级参考文献35

共引文献20

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈