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Box-Behnken设计-响应面法优化三棱总黄酮的分离纯化工艺研究 预览

Study on the Separation and Purification Technology of Total Flavonoids from Sparganium stoloni by Box-Behnken Design-response Surface Methodology
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摘要 目的:优化三棱总黄酮的纯化工艺。方法:采用大孔吸附树脂分离纯化法,以上样液pH、洗脱液流速、洗脱液浓度为考察因素,总黄酮纯化率为评价指标,在单因素试验的基础上采用Box-Behnken 设计-响应面法优化三棱总黄酮的纯化工艺,并进行验证试验。结果:最优纯化工艺为上样液pH为4.8、洗脱液流速为2.0 BV/h、洗脱液浓度为72%;验证试验中3 批样品纯化后的总黄酮纯化率为72.34%(RSD=1.77%,n=3),与预测值(73.99%)的相对误差为2.13%。结论:优选的纯化工艺方法稳定、可行,可用于三棱总黄酮的纯化。 OBJECTIVE: To optimize the purification technology of total flavonoids from Sparganium stoloniferum. METHODS:Separation and purification by macroporus adsorption resin,using sample solution pH,flow rate and concentration of eluent,the purification rate of total flavonoids as evalution indexes,the purification technology of total flavonoids from S. stoloniferum were optimized by Box-Behnken design-response surface methodology based on single factor test. Validation test was conducted. RESULTS: The optimal purification technology was sample solution pH 4.8, flow rate of eluent 2.0 BV/h, concentration of eluent 72%. The purification rate of total flavonoids in 3 batches of samples was 72.34%(RSD=1.77%,n=3) in validation test,relative errors of which to predicted value(73.99%)was 2.13%. CONCLUSIONS:The optimal purification technology is stable and feasible,and can be used for the purification of total flavonoids from S. stoloniferum.
作者 匡宇 赵永艳 何沛煜 张军银 周燕 彭腾 余成浩 KUANG Yu;ZHAO Yongyan;HE Peiyu;ZHANG Junyin;ZHOU Yan;PENG Teng;YU Chenghao(School of Pharmacy,Chengdu University of TCM/National Key Lab Breeding Base of TCM Resource System Study and Development Utilization/Key Lab of TCM Standardization,Ministry of Education,Chengdu 611137,China;College of Basic Medicine,Chengdu University of TCM,Chengdu 611137,China)
出处 《中国药房》 CAS 北大核心 2019年第11期1502-1506,共5页 China Pharmacy
基金 国家自然科学基金资助项目(No.81673878).
关键词 三棱总黄酮 纯化工艺 大孔吸附树脂 Box-Behnken设计-响应面法 Total flavonoids of Sparganium stoloniferum Purification technology Macroporous adsorption resin Box-Behnken design-response surface methodology
作者简介 匡宇,硕士研究生。研究方向:药物化学。E-mail:605098083@qq.com;通信作者:彭腾,教授,博士。研究方向:药物化学。E-mail:56649923@qq.com.
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