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冰菜盐胁迫下的转录组分析 预览

Transcriptome Analysis of Mesembryanthemum crystallinum under Salt Stress
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摘要 为了解冰菜(Mesembryanthemum crystallinum)叶片抗盐相关基因组学,利用Illumina Hi-seq TM2500高通量测序技术研究冰菜叶片在400mmolL–1NaCl胁迫下转录组基因的差异表达。结果表明,从400mmolL^–1NaCl胁迫和对照的冰菜叶片中共获得13.01Gb Clean data,Q30碱基均大于90.08%。共获得123个差异表达基因(DEGs),包括73个上调基因,50个下调基因,其中功能注释的基因有96个。根据Unigene库序列进行GO、COG和KEGG注释,筛选出8个与抗盐性相关差异表达基因,植物激素代谢相关基因,脱落酸8'-羟基化酶、吲哚-3-乙酰酸酰胺合成酶和茉莉酮酸酯ZIM结构域蛋白基因均下调表达,生长素响应蛋白、细胞分裂素合酶基因则上调表达,糖代谢相关基因棉子糖合成酶基因上调表达,质膜H^+-ATPase基因上调表达,脱水蛋白基因下调表达。这为冰菜耐盐基因组学和分子生物学的研究奠定基础。 In order to understand the salt related genomics in leaves of Mesembryanthemum crystallinum, the expression of transcriptome genes was studied under 400 mmol L 1 NaCl stress by using Illumina Hiseq TM2500 high-throughput sequencing technology. The results showed that there were 13.01 Gb Clean Data from the leaves treated with 400 mmol L^-1 NaCl and untreated, the Q30 base was more than 90.08%. Among them, there were 123 differential expression genes (DEGs), including 73 up-regulated genes and 50 down-regulated genes, in which 96 genes were annotated. The eight salt-resistance DEGs of were screened out based on GO, COG and KEGG annotation. The plant hormone metabolism-related genes, including abscisic acid 8 -hydroxylase gene, indole-3-acetamide synthase gene, jasmonate ZIM structure domain protein gene were down-regulated, auxin responsive protein and cytokinin synthase genes were up-regulated. The sugar metabolism-related raffinose synthase genes was up-regulated, and plasma membrane H^+-ATPase and dehydrated protein genes were up and down-regulated, respectively. These would provide a foundation for studing on salt tolerance genomics and molecular biology of M. crystallinum.
作者 练冬梅 赖正锋 姚运法 林碧珍 洪建基 LIAN Dong-mei;LAI Zheng-feng;YAO Yun-fa;LIN Bi-zhen;HONG Jian-ji(Subtropical Agriculture Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou 363005,Fujian,China)
出处 《热带亚热带植物学报》 CAS CSCD 北大核心 2019年第3期279-284,共6页 Journal of Tropical and Subtropical Botany
基金 福建省公益类科研院所专项(2018R1101025-2)资助.
关键词 冰菜 盐胁迫 叶片 转录组 差异表达基因 Mesembryanthemum crystallinum Salt stress Leaf Transcriptome DEGs
作者简介 练冬梅,女,助理研究员,主要从事特色蔬菜研究。E-mail:woshildm1987@163.com;通信作者:洪建基,E-mail:76227508@qq.com.
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