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SS-31对蛛网膜下腔出血大鼠模型早期脑损伤及NLRP3炎性小体的影响 预览

Effect of SS-31 on brain injury in subarachnoid hemorrhage of rats models and NLRP3 inflammatory corpuscles
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摘要 目的探讨线粒体靶向抗氧化肽SS-31对Nod样受体蛋白3(NLRP3)炎性小体的影响以及对蛛网膜下腔出血(SAH)早期脑损伤的作用。方法将120只成年雄性大鼠随机分为4组:假手术组(Sham组)、SAH组、SAH+载体组(SAH+V组)和SAH+SS-31组,对每组大鼠进行神经功能评估,脑含水量和活性氧(ROS)测量,蛋白质免疫印迹分析NLRP3、凋亡相关斑点样蛋白(ASC)和caspase-1的蛋白表达情况,采用退化神经元染色(Fluoro-Jade C)、末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)和DNA聚合酶I介导的生物素标记的(dATP)缺口平移(PANT)染色分析神经细胞死亡情况。结果 SS-31治疗可以增加大鼠神经功能评分,使SAH损伤后的脑水肿减少,ROS的含量显著降低,抑制SAH后大鼠NLRP3炎性小体,显著降低IL-1β和IL-18的表达,显著减少Fluoro-Jade C阳性细胞、PANT阳性细胞和TUNEL阳性细胞的数量。结论线粒体靶向抗氧化肽SS-31可抑制NLRP3炎性小体,并对蛛网膜下腔出血早期脑损伤具有保护作用。 Objective To investigate the effect of mitochondria-targeted antioxidant peptide SS-31 on NLRP3 inflammatory corpuscles and early brain injury in subarachnoid hemorrhage(SAH). Methods 120 adult male rats were randomly divided into 4 groups, including sham operation group(Sham group), SAH group, SAH+carrier group(SAH+V group) and SAH+SS-31 group. Neurological function was assessed, brain water content and reactive oxygen species(ROS) were measured, immunoblotting analysis of NLRP3, ASC and caspase-1 protein expression was performed. Fluoro-Jade C, TUNEL and PANT staining were used to analyze neuronal cell death. Results SS-31 treatment was shown to be able to increase the neurological function score of rats, reducing brain edema after SAH injury, significantly reducing ROS content, inhibit NLRP3 inflammatory body in rats after SAH, and significantly reducing the expression of IL-1β and IL-18. Meanwhile, the number of Fluoro-Jade C positive cells, PANT positive cells and TUNEL positive cells were observed to be significantly reduced. Conclusion Mitochondria-targeted antioxidant peptide SS-31 can inhibit NLRP3 inflammatory corpuscles and protect against early brain injury in subarachnoid hemorrhage.
作者 彭华 唐晓平 孙浩耕 赵龙 杨彬彬 张涛 PENG Hua;TANG Xiaoping;SUN Haogeng;ZHAO Long;YANG Binbin;ZHANG Tao(Department of Neurosurgery,Affiliated Hospital of North Sichuan Medical College,Nanchong Sichuan 637000,China)
出处 《新疆医科大学学报》 CAS 2019年第6期743-747,共5页 Journal of Xinjiang Medical University
基金 川北医学院科研项目(CBY17-A-ZD09).
关键词 SS-31 NLRP3 蛛网膜下腔出血 早期脑损伤 SS-31 NLRP3 subarachnoid hemorrhage early brain injury
作者简介 彭华(1978-),硕士,主治医师,研究方向:脑血管疾病,E-mail:ph9840@163.com.
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