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红树植物异色拟盘多毛孢内生真菌次级代谢产物及其生物活性 预览

The secondary metabolites of the endophytic fungi Pestalotiopsis versicolor from mangrove and their biological activities
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摘要 目的研究红树植物异色拟盘多毛孢内生真菌Pestalotiopsis versicolor的次级代谢产物及其生物活性。方法真菌固体发酵产物采用硅胶、Sephadex LH-20、制备薄层进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。PNPG法测定化合物对α-葡萄糖苷酶的抑制能力。结果从中分离得到8个化合物,分别鉴定为3-(4-甲苯氧基)丙酸(1)、对羟基苯乙醇(2)、2-苯乙醇(3)、3β,5α,9α-三羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(4)、麦角甾醇(5)、麦角甾4,6,8(14),22-四烯-3酮(6)、(1aS,3R,4R,4aR,6S,7R,8aS)-7-chloro-3,6-dihydroxy-3,4a,8,8-tetramethyl-octahydro-1aH-naphtho[1-b]oxirene-4-carboxylic acid(7)、cepharosporolides G(8)。化合物1在质量浓度为6.25 mg/mL时对α-葡萄糖苷酶具有较弱的抑制活性。结论化合物7为首次从拟盘多毛孢属内生真菌的固体发酵产物中分离得到,化合物5为首次从异色拟盘多毛孢内生真菌的固体发酵产物中分离得到。化合物1有较弱的生物活性。 AIM To study the secondary metabolites of the endophytic fungi Pestalotiopsis versicolor from mangrove and their biological activities.METHODS The solid fermentation was isolated and purified by silica,Sephadex LH-20 and preparative thin layer chromatography,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their inhibition againstα-glucosidase was determined by PNPG method.RESULTS Eight compounds were isolated and identified as 3-(p-tolyloxy)propanoic acid(1),4-(2-hydroxyethyl)phenol(2),2-phenylethanol(3),3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-diene-6-one(4),ergosterol(5),ergosta-4,6,8(14),22-tetraen-3-one(6),(1aS,3R,4R,4aR,6S,7R,8aS)-7-chloro-3,6-dihydroxy-3,4a,8,8-tetramethyl-octahydro-1aH-naphtho[1-b]oxirene-4-carboxylic acid(7),cepharosporolides G(8).Compound 1 showed weak inhibition againstα-glucosidase at the concentration of 6.25 mg/mL.CONCLUSION Compound 7 is isolated from the solid fermentation of Pestalotiopsis genus for the first time,while compound 5 is isolated from the solid fermentation of P.versicolor for the first time.Compound 1 has weak biological activities.
作者 李晓芳 田颖超 董乾 耿文跃 宋小妹 罗都强 LI Xiao-fang;TIAN Ying-chao;DONG Qian;GENG Wen-yue;SONG Xiao-mei;LUO Du-qiang(College of Life Science,Hebei University,Baoding 071002,China;College of Chemistry & Environmental Science,Hebei University,Baoding 071002,China)
出处 《中成药》 CAS CSCD 北大核心 2019年第5期1054-1058,共5页 Chinese Traditional Patent Medicine
基金 河北省人力资源和社会保障厅留学人员科技活动项目择优资助经费(C2012003027).
关键词 红树植物 异色拟盘多毛孢内生真菌 次级代谢产物 化学成分 分离鉴定 生物活性 mangrove Pestalotiopsis versicolor secondary metabolites chemical constituents isolation andidentification biological activities
作者简介 李晓芳(1993—),女,硕士生,主要从事天然产物开发及新药研究。E-mail:342292302@qq.com;通信作者:宋小妹(1984-),女,讲师,主要从事天然产物开发及新药研究。E-mail:songxiaomei@hbu.edu.cn;通信作者:罗都强(1965-),男,教授,主要从事天然产物开发及新药研究。E-mail:duqiangluo@163.com.
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