以纳塔栎母树2年生的嫩枝为插穗,采用不同植物生长调节剂对插穗进行处理,测定插穗生根率、平均生根数、根长和生根指数,选取生根效果最好的ABT处理用于测定插穗在生根过程中可溶性糖、可溶性蛋白含量、相关酶活性、MDA含量以及内源激素含量变化。结果表明:200mg/kg ABT处理可促进插穗生根,处理后的插穗生根率、平均生根数、根长及生根指数分别为33.30%、1.91条/株、2.71cm和1.693,均显著高于200mg/kg IAA及200mg/kg NAA处理。插穗在生根过程中,可溶性糖含量呈现先下降后上升再下降趋势,而可溶性蛋白总体上呈现先上升后下降趋势;POD和PPO活性在根原基诱导期和不定根伸长期出现2个峰值且较对照组峰值提前;SOD活性和MDA含量总体上呈现先上升后下降趋势;内源激素含量变化趋势不一但都高于对照组。植物生长调节剂处理能提高插穗内营养物质含量、相关酶活性及内源激素含量,对插穗生根有促进作用。
Softwood cuttings from 2a Quercus nuttallii mother plants were collected and treated with different plant growth regulators to determine the rooting rate, average number of new roots, length of new root and rooting index of cuttings. Cuttings treated with ABT solution which has the best rooting promoting efficiency was selected to determine the changes in soluble sugar content, soluble protein content, related enzymic activities, MDA content and endogenous hormones content during the rooting process of cuttings. The results showed that cuttings treated with 200 mg/kg ABT could promote rooting capacity. The rooting rate, average number of new roots, length of new root and rooting index of the cuttings treated with 200 mg/kg ABT were 33.30%, 1.91 per plant, 2.71 cm and 1.693 respectively, which were apparently higher than cuttings treated with 200 mg/kg IAA and 200 mg/kg NAA. During the rooting process, the content of soluble sugar decreased at first, then increased and then decreased, while the content of soluble protein increased at first and then decreased as a whole;There appeared 2 peaks in induction period of root primordium and elongation period of adventitious root in the activity of POD and PPO, being earlier than the control group;The activity of SOD and content of MDA both had a trend of increasing first and then decreasing;While the endogenous hormone content had a different trend but being higher than that of the control group. Plant growth regulators treatment can increase the nutrient content, related enzymic activities and endogenous hormones content in the cuttings, which can promote the rooting capacity of cuttings.
Journal of Southwest Forestry College
plant growth regulators