Objective To investigate the effects of pentamer (PTX)3 on apoptosis and secretion of vasoactive substances in human umbilical vascular endothelial cells (HUVECs) stimulated by AngⅡ. Methods After stimulating HUVECs with 10 -7 mol/L AngⅡ for 0, 12, 24 and 48 hours in vitro, the expression of PTX3 protein in HUVECs was detected by Western blot. PTX3 siRNA was transfected into HUVECs by Lipofectamine 2000, and its transfection effect was detected by Western blot. HUVECs were randomly divided into blank group (without any treatment), AngⅡ group (10 -7 mol/L AngⅡ stimulation), AngⅡ+ siNC group (10 -7 mol/L AngⅡ stimulation after transfection of negative control sequence) and AngⅡ+ siPTX3 group 10 -7 mol/L AngⅡ stimulation after transfection of PTX3 siRNA). Cell proliferation was detected by MTT method, cell apoptosis was detected by flow cytometry, and the expression of endothelin (ET)-1 and inducible nitric oxide synthase (iNOS) mRNA in cells of each group were detected by RT-PCR. Results The expression of PTX3 protein was increased significantly with the prolongation of AngⅡ stimulation time. PTX3 siRNA was successfully transfected to down-regulate PTX3 expression induced by AngⅡ. AngⅡ stimulation induced HUVECs to decrease proliferation, increase apoptosis, and increase the expressions of ET-1 and iNOS. Down-regulation of PTX3 expression attenuated HUVECs injury induced by AngⅡ, promoted HUVECs proliferation, inhibited apoptosis, and down-regulated the expressions of ET-1 and iNOS. Conclusions The expression of PTX3 is increased in HUVECs stimulated by AngⅡ, and its down regulation can inhibit the apoptosis induced by AngⅡ and the secretion of vasoactive substances.
Chinese Journal of Gerontology