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菜豆A18-1 60Co-γ射线诱变和突变体库的构建 预览

Mutagenesis of Snap Bean Cultivar A18-1 by 60Co-γ-rays and Mutant Library Construction
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摘要 分别用辐射剂量为0、50、100、150、200、250Gy的60Co-γ射线处理菜豆品种A18-1的成熟干种子,根据辐射剂量和种子的相对出苗率建立线性回归方程,研究60Co-γ射线对菜豆的辐射诱变效应。结果表明,辐射处理的半致死剂量为126Gy。用辐射剂量为126Gy的60Co-γ射线处理A18-1的干种子,对M2进行田间表型筛选,共筛选到279株突变株,占M2诱变群体总数的1.83%。构建了包括叶、茎、花和育性、荚、生长习性和株型、黄化、熟期等性状变异类型的突变体,可为今后菜豆遗传改良和功能基因组学研究提供新的材料。 The matured dry seeds of snap bean cultivars A18-1 were treated with 60Co-γ-rays at 0,50,100,150,200,250 Gy,respectively.According to the radiation dose and relative seed emergence rate,a linear regression equation was established to study the radiation mutagenesis of 60Co-γ-rays on snap bean.The result indicated that the semi-lethal dose was 126 Gy.Then the matured dry seeds of A18-1 were treated with radiation dose of 126 Gy,and M2 generation was screened by field phenotypic performance and a total of 279 mutant strains were screened,accounting for 1.83%of the total M2 mutagenized populations.Mutants including leaf,stem,flower,pod,growth habit,plant type and ripe stage,etc.character variation types have been constructed,which can provide new germplasm resources for snap bean genetic improvement and functional genomics research.
作者 陈其福 刘畅 斯琴图雅 赵弘韬 刘大军 冯国军 Chen Qi-fu;Liu Chang;SiQin Tu-ya;Zhao Hong-tao;Liu Da-jun;Feng Guo-jun(Crop Institute,Heilongjiang University,Harbin 150080,Heilongjiang,China;Technical Physics Institute,HeilongjiangAcademy of Sciences,Harbin 150086,Heilongjiang,China)
出处 《中国蔬菜》 北大核心 2019年第5期38-44,共7页 China Vegetables
基金 哈尔滨市应用技术研究与开发项目(2017RAGXJ003) 黑龙江省科学院科学研究基金项目(KY2018WL01).
关键词 60Co-γ射线 诱变 菜豆 突变体库 表型筛选 60Co-γ-rays Mutagenesis Snap bean Mutant library Phenotypic screening
作者简介 陈其福,男,硕士研究生,专业方向:寒区作物种质资源创新及利用,E-mail:chenqf63@163.com;通讯作者(Corresponding author):冯国军,研究员,硕士生导师,专业方向:园艺学,E-mail:feng998@126.com.
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